Online citations, reference lists, and bibliographies.
← Back to Search

Bovine Pregnancy Associated Glycoproteins Can Alter Selected Transcripts In Bovine Endometrial Explants.

Rhianna M Wallace, Mariah Hart, T. Egen, Amanda L Schmelzle, M. F. Smith, K. Pohler, J. Green
Published 2019 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
This study was designed to examine changes in target transcript abundance in endometrial explants exposed to pregnancy-associated glycoproteins (PAGs). Endometrial explants from pregnant and non-pregnant heifers collected on day18 (day 0: day of insemination) were incubated in the absence or presence of PAGs (15 μg/ml). The PAGs represented a mixture comprised of approximately equal amounts of bovine PAGs 4, 6, and 9. Samples were harvested for RNA extraction after 24 h or 96 h of incubation. Transcript abundance for target genes related to prostaglandin synthesis (PTGES), a chemokine (CXCL5) and tissue remodeling (EMMPRIN; MMPs 1, 2, 3, 7, 8, and 9; PLAU; SPP1; TIMP1 and TIMP2) were analyzed by quantitative PCR. Changes in relative transcript abundance for MMP1, MMP3, MMP7, PLAU, EMMPRIN and SPP1 were observed after PAG exposure in both non-pregnant and pregnant endometrium (P < 0.05). However, some of the transcripts associated with tissue remodeling were altered only at certain time points (either 24 h or 96 h). The transcript for bovine CXCL5 was increased in non-pregnant endometrium four- and six-fold at 24 h and 96 h of PAG exposure, respectively (P < 0.05); in pregnant endometrium, only the 24 h incubation period exhibited an elevation in CXCL5 (P < 0.05). In non-pregnant endometrium, both PTGES and MMP9 were elevated after exposure to PAGs for 24 h (P < 0.05) but not in the other samples. Some interferon-responsive transcripts (IFI6, ISG15) were found to be more abundant (P < 0.05) in pregnant endometrium after 96 h exposure to PAGs compared to endometrium that had not been exposed to the PAGs. Likewise, ISG15 message was elevated (P = 0.06) in non-pregnant endometrium after 24 h incubation with PAGs. These results indicate that the PAGs used in this experiment were able to induce changes in endometrial transcripts encoding for proteins associated with matrix remodeling as well as chemokine production and prostaglandin release.
This paper references
10.3168/jds.2015-10192
Circulating concentrations of bovine pregnancy-associated glycoproteins and late embryonic mortality in lactating dairy herds.
K. Pohler (2016)
10.3168/JDS.2007-0276
Accuracy of a pregnancy-associated glycoprotein ELISA to determine pregnancy status of lactating dairy cows twenty-seven days after timed artificial insemination.
E. Silva (2007)
10.1023/A:1013908332232
Matrix Metalloproteinases and Cellular Fibrinolytic Activity
H. R. Lijnen (2004)
10.1051/RND:19930309
Peripheral concentrations of a 60-kDa pregnancy serum protein during gestation and after calving and in relationship to embryonic mortality in cattle.
M. Mialon (1993)
10.1016/S0165-2427(99)00031-8
In vitro effect of ketone bodies, glucocorticosteroids and bovine pregnancy-associated glycoprotein on cultures of bone marrow progenitor cells of cows and calves.
D. Hoeben (1999)
10.1042/BJ2780179
The purification of tissue inhibitor of metalloproteinases-2 from its 72 kDa progelatinase complex. Demonstration of the biochemical similarities of tissue inhibitor of metalloproteinases-2 and tissue inhibitor of metalloproteinases-1.
R. Ward (1991)
10.1177/10454411930040020401
Matrix metalloproteinases: a review.
H. Birkedal-Hansen (1993)
10.1093/MOLBEV/MSG217
Aspartic proteinase phylogeny and the origin of pregnancy-associated glycoproteins.
A. Hughes (2003)
10.1530/REP-14-0485
Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy.
Rhianna M Wallace (2015)
10.1016/bs.pmbts.2017.02.005
Biochemical and Biological Attributes of Matrix Metalloproteinases.
N. Cui (2017)
10.1095/BIOLREPROD53.2.302
Tissue inhibitors of metalloproteinases in endometrium of ovariectomized steroid-treated ewes and during the estrous cycle and early pregnancy.
A. Hampton (1995)
10.1530/BIOSCIPROCS.3.003
Matrix metalloproteinases and their tissue inhibitors at the ovine trophoblast-uterine interface.
L. Salamonsen (1995)
10.1002/jcp.25430
Intricate Functions of Matrix Metalloproteinases in Physiological and Pathological Conditions
R. Mittal (2016)
Alterations in bovine neutrophil function during the periparturient period.
M. Kehrli (1989)
10.1016/0092-8674(94)90007-8
Integrin αvβ3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels
P. Brooks (1994)
10.1530/JRF.0.0980583
Production of matrix metalloproteinase 1 (interstitial collagenase) and matrix metalloproteinase 2 (gelatinase A: 72 kDa gelatinase) by ovine endometrial cells in vitro: different regulation and preferential expression by stromal fibroblasts.
L. Salamonsen (1993)
10.1016/0090-6980(95)00108-5
Prostaglandin F2 alpha, progesterone and oxytocin production by cultured bovine luteal cells treated with prostaglandin E2 and pregnancy-specific protein B.
R. D. Del Vecchio (1995)
10.1530/REP-13-0143
Specific expression patterns and cell distribution of ancient and modern PAG in bovine placenta during pregnancy.
E. Touzard (2013)
10.1006/METH.2001.1262
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak (2001)
10.1210/JCEM.79.2.8045973
Matrix metalloproteinase production by cultured human endometrial stromal cells: identification of interstitial collagenase, gelatinase-A, gelatinase-B, and stromelysin-1 and their differential regulation by interleukin-1 alpha and tumor necrosis factor-alpha.
T. J. Rawdanowicz (1994)
10.1016/S0303-7207(02)00054-0
Ovarian tissue remodeling: role of matrix metalloproteinases and their inhibitors
M. Smith (2002)
10.1111/j.1749-6632.1994.tb24722.x
Regulation of Matrix Metalloproteinase Activity a
G. Murphy (1994)
10.1111/j.1699-0463.1999.tb01524.x
Mechanisms for pro matrix metalloproteinase activation
G. Murphy (1999)
10.1016/S0090-6980(98)00030-6
Effects of Luteinizing Hormone (LH), PGE2, 8-Epi-PGE1, 8-Epi-PGF2α, Trichosanthin and Pregnancy Specific Protein B (PSPB) on Secretion of Prostaglandin (PG) E (PGE) or F2α (PGF2α) In Vitro by Corpora Lutea (CL) From Nonpregnant and Pregnant Cows
Y. S. Weems (1998)
10.1016/0143-4004(92)90025-O
Current topic: the synepitheliochorial placenta of ruminants: binucleate cell fusions and hormone production.
F. Wooding (1992)
10.1095/BIOLREPROD45.1.1
Purification and characterization of a bovine pregnancy-associated glycoprotein.
A. Zoli (1991)
10.1095/BIOLREPROD35.4.936
Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation.
R. Sasser (1986)
10.1016/S0165-2427(98)00215-3
Pregnancy-associated glycoprotein and decreased polymorphonuclear leukocyte function in early post-partum dairy cows.
H. Dosogne (1999)
10.1073/PNAS.93.8.3509
Suppression of apoptosis by basement membrane requires three-dimensional tissue organization and withdrawal from the cell cycle.
N. Boudreau (1996)
10.1016/S0090-6980(98)00003-3
Effect of luteinizing hormone (LH), PGE2, 8-EPI-PGE1, 8-EPI-PGE2, trichosanthin, and pregnancy specific protein B (PSPB) on secretion of progesterone in vitro by corpora lutea (CL) from nonpregnant and pregnant cows.
Y. S. Weems (1998)
10.1095/biolreprod.112.102152
The Role of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) in the Regulation of Bovine Endometrial Cell Functions1
Birendra Mishra (2012)
10.1007/s00418-003-0507-6
In bovine binucleate trophoblast giant cells, pregnancy-associated glycoproteins and placental prolactin-related protein-I are conjugated to asparagine-linked N-acetylgalactosaminyl glycans
K. Klisch (2003)
10.1091/MBC.5.4.439
The extracellular matrix ligands fibronectin and tenascin collaborate in regulating collagenase gene expression in fibroblasts.
P. Tremble (1994)
10.2527/1995.7392662X
Effect of pregnancy-specific protein B on luteal cell progesterone, prostaglandin, and oxytocin production during two stages of the bovine estrous cycle.
R. D. Del Vecchio (1995)
10.1073/PNAS.94.24.12809
The diversity and evolutionary relationships of the pregnancy-associated glycoproteins, an aspartic proteinase subfamily consisting of many trophoblast-expressed genes.
S. Xie (1997)
10.1111/j.1582-4934.2005.tb00355.x
Matrix metalloproteinases and angiogenesis
J. Rundhaug (2005)
10.1095/BIOLREPROD26.5.925
Detection and partial characterization of two bovine pregnancy-specific proteins.
J. E. Butler (1982)
10.1126/science.7531366
Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix
N. Boudreau (1995)
10.1016/J.THERIOGENOLOGY.2004.07.011
The establishment of an ELISA for the detection of pregnancy-associated glycoproteins (PAGs) in the serum of pregnant cows and heifers.
J. Green (2005)
10.1016/S0092-8674(00)81235-0
Localization of Matrix Metalloproteinase MMP-2 to the Surface of Invasive Cells by Interaction with Integrin αvβ3
P. Brooks (1996)
The role of the binucleate cell in ruminant placental structure.
F. Wooding (1982)
10.1210/ENDO.140.1.6608
Pregnancy-specific protein B induces release of an alpha chemokine in bovine endometrium.
K. J. Austin (1999)
Structure and function of placental binucleate ('giant') cells.
F. Wooding (1982)
10.1095/biolreprod.102.013573
Osteopontin Is Synthesized by Uterine Glands and a 45-kDa Cleavage Fragment Is Localized at the Uterine-Placental Interface Throughout Ovine Pregnancy1
G. Johnson (2003)
10.1530/JRF.0.1070131
Bovine luteal cell production in vitro of prostaglandin E2, oxytocin and progesterone in response to pregnancy-specific protein B and prostaglandin F2 alpha.
R. D. Del Vecchio (1996)
10.1186/1471-2164-10-185
Characterization of the bovine pregnancy-associated glycoprotein gene family – analysis of gene sequences, regulatory regions within the promoter and expression of selected genes
B. P. V. Telugu (2008)
10.1159/000468617
Cooperation between matrix metalloproteinases and the plasminogen activator-plasmin system in tumor progression.
Y. DeClerck (1996)
10.1051/RND:19940107
Detection of pregnancy by radioimmunoassay of a pregnancy serum protein (PSP60) in cattle.
M. Mialon (1994)
10.1530/JRF.0.0620015
Localization of ovine placental lactogen in sheep placentomes by electron microscope immunocytochemistry.
F. B. Wooding (1981)
10.1002/MRD.20017
Quantitative analysis throughout pregnancy of placentomal and interplacentomal expression of pregnancy‐associated glycoproteins‐1 and ‐9 in the cow
O. Patel (2004)
10.1073/PNAS.88.22.10247
Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family.
S. Xie (1991)
10.1016/S0093-691X(03)00217-6
Involvement of the plasminogen activation system in cow endometritis.
S. Moraitis (2004)
10.1095/biolreprod62.6.1624
Pregnancy-Associated Bovine and Ovine Glycoproteins Exhibit Spatially and Temporally Distinct Expression Patterns During Pregnancy1
J. Green (2000)
10.1186/1477-7827-8-60
Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle
Birendra Mishra (2010)
10.1016/0739-7240(96)00048-3
Plasma bovine placental lactogen concentration throughout pregnancy in the cow; relationship to stage of pregnancy, fetal mass, number and postpartum milk yield.
O. Patel (1996)
10.1007/BF02409011
Phagocytosis and intracellular digestion of collagen, its role in turnover and remodelling
V. Everts (2005)
10.1095/BIOLREPROD46.1.83
Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis.
A. Zoli (1992)
10.1530/JRF.0.0590425
Binucleate cell migration in the bovine placentome.
F. Wooding (1980)
10.1016/j.mce.2010.09.009
Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy
S. Ulbrich (2011)
10.1016/0093-691X(90)90644-9
Partial characterization of the immunosuppressive properties of pregnancy-specific protein B (PSPB)
M. M. Dunbar (1990)
10.1017/S0022029900004052
Chemiluminescence of bovine polymorphonuclear leucocytes during the periparturient period and relation with metabolic markers and bovine pregnancy-associated glycoprotein.
D. Hoeben (2000)
10.1002/MRD.20119
Temporospatial expression of placental lactogen and prolactin‐related protein‐1 genes in the bovine placenta and uterus during pregnancy
O. Patel (2004)
10.1038/nprot.2008.73
Analyzing real-time PCR data by the comparative CT method
Thomas D. Schmittgen (2008)
10.1002/JEZ.1402660609
Comparative placentation in ungulates.
G. King (1993)
10.1053/PLAC.2001.0633
Matrix metalloproteinases (MMP-2 and MMP-9) and tissue inhibitor-2 of matrix metalloproteinases (TIMP-2) in the placenta and interplacental uterine wall in normal cows and in cattle with retention of fetal membranes.
I. Walter (2001)
10.1016/J.PROSTAGLANDINS.2007.08.002
Effect of luteinizing hormone (LH), pregnancy-specific protein B (PSPB), or arachidonic acid (AA) on secretion of progesterone and prostaglandins (PG) E (PGE; PGE(1) and PGE(2)) and F(2alpha) (PGF(2alpha)) by ovine corpora lutea of the estrous cycle or pregnancy in vitro.
Y. S. Weems (2007)
10.1002/AJA.1001590305
An electron microscopic study of implantation in the cow.
D. Wathes (1980)
10.2527/jas.2013-6348
Circulating bovine pregnancy associated glycoproteins are associated with late embryonic/fetal survival but not ovulatory follicle size in suckled beef cows.
K. Pohler (2013)
10.1016/J.PLACENTA.2004.10.014
Light and electron microscope immunocytochemical studies of the distribution of pregnancy associated glycoproteins (PAGs) throughout pregnancy in the cow: possible functional implications.
F. Wooding (2005)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar