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Influence Of Cytotoxic Doses Of 4-hydroxynonenal On Selected Neurotransmitter Receptors In PC-12 Cells.

M. A. Siddiqui, G. Singh, M. P. Kashyap, V. Khanna, S. Yadav, D. Chandra, A. Pant
Published 2008 · Chemistry, Medicine

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Effect of 4-hydroxynonenal (HNE), a long-chain alpha, beta unsaturated aldehyde product, generated by the oxidation of omega-6 polyunsaturated fatty acids on the sensitivity of selected neurotransmitter receptors was studied in PC-12 cells. Cytotoxicity profiling was carried out at varying concentrations of HNE (0.1-50microM) for 30min to 24h. Trypan blue dye exclusion, MTT, LDH release and neutral red uptake (NRU) assays were carried out to assess the cytotoxicity of HNE. Cytotoxic response was found to be significant at 2h of exposure. Cytotoxicity of HNE at 50microM was exerted even at 90min. HNE 10-50microM was found to be cytotoxic, whereas, 2-5microM causes physiological stress only and 1-0.1microM non-cytotoxic. Effect on dopamine, cholinergic, serotonin and benzodiazepine receptors was studied at varying concentrations of HNE (1, 10, 25 and 50microM for 1-8h). A significant decrease in binding of 3H-QNB, 3H-Fluinitrazepam and 3H-Ketanserin, known to label cholinergic (muscarinic), benzodiazepine and serotonin (5HT(2A)) receptors respectively was observed at 1h exposure of PC-12 cells to HNE at 25 and 50microM concentrations. The decrease in the binding of (3)H-Spiperone, known to label dopamine (DA-D2) receptors was evident at 4h of exposure of PC-12 cells to HNE. The decrease in the binding with DA-D2 receptors continued till 8h. Effect on the binding of (3)H-Fluinitrazepam and 3H-Ketanserin appeared to be maximum at 25 and 50microM concentrations of HNE for 4h and 8h. The PC-12 cells appear to be vulnerable to cytotoxic concentrations of HNE. Experimental HNE exposure provides an intriguing model of toxicant-cell interactions involving neurotransmitter receptors in HNE neurotoxicity.
This paper references
Differential sensitivity to 4-hydroxynonenal for normal and malignant mesenchymal cells
S. Borović (2007)
Metabolism of lipid peroxidation product, 4-hydroxynonenal (HNE) in rat erythrocytes: role of aldose reductase.
S. Srivastava (2000)
A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90)
E. Borenfreund (1985)
Geldanamycin increases 4-hydroxynonenal (HNE)-induced cell death in human retinal pigment epithelial cells
K. Kaarniranta (2005)
Oligomerization of Escherichia coli enterotoxin b through its C-terminal hydrophobic α-helix
V. Labrie (2001)
Neuroprotection by tamoxifen in focal cerebral ischemia is not mediated by an agonist action at estrogen receptors but is associated with antioxidant activity
Yonghua Zhang (2007)
Trypan blue uptake by chinese hamster ovary cultured epithelial cells: a cellular model to study Escherichia coli STb enterotoxin.
H. Beausoleil (2002)
Role of oxidative stress in paraquat‐induced dopaminergic cell degeneration
A. McCormack (2005)
Activation of the canonical Wnt pathway by the antipsychotics haloperidol and clozapine involves dishevelled‐3
L. Sutton (2007)
In vitro cytotoxicity of polycyclic aromatic hydrocarbon residues arising through repeated fish fried oil in human hepatoma Hep G2 cell line.
M. Pandey (2006)
Adaptive response induced by lipid peroxidation products in cell cultures
Z. Chen (2006)
Alterations of metabolic activity in human osteoarthritic osteoblasts by lipid peroxidation end product 4-hydroxynonenal
Q. Shi (2006)
Effect of protein malnutrition on the neurobehavioural toxicity of styrene in young rats
V. Khanna (1994)
[The expression of 4-hydroxy-2-nonenal caused by cigarette smoke condensate exposure in human bronchial epithelial cells and the interventional effects of ginkgolide B].
L. Yu (2006)
Formation of trans-4-hydroxy-2-nonenal- and other enal-derived cyclic DNA adducts from ω-3 and ω-6 polyunsaturated fatty acids and their roles in DNA repair and human p53 gene mutation
F. Chung (2003)
4-Hydroxynonenal Induces Adaptive Response and Enhances PC12 Cell Tolerance Primarily through Induction of Thioredoxin Reductase 1 via Activation of Nrf2*
Z. Chen (2005)
N-Acetylcysteine and ebselen but not nifedipine protected cerebellar granule neurons against 4-hydroxynonenal-induced neuronal death
M. Arakawa (2007)
Insulin neuroprotection against oxidative stress in cortical neurons--involvement of uric acid and glutathione antioxidant defenses.
A. Duarte (2005)
Phosphatase inhibitors potentiate 4-hydroxynonenal-induced phospholipase D activation in vascular endothelial cells.
V. Natarajan (1997)
Possible roles of nitric oxide and redox cell signaling in metal-induced toxicity and carcinogenesis: a review.
G. Buzard (2000)
Mouse aldo-keto reductase AKR7A5 protects V79 cells against 4-hydroxynonenal-induced apoptosis.
D. Li (2006)
Differential coupling of α7 and non‐α7 nicotinic acetylcholine receptors to calcium‐induced calcium release and voltage‐operated calcium channels in PC12 cells
Jane A. Dickinson (2007)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
Dopamine D2 receptor gene expression and binding sites in adrenal medulla and pheochromocytoma [published erratum appears in J Clin Endocrinol Metab 1994 Oct;79(4):1165]
C. Pupilli (1994)
The involvement of lipid radical cycles and the adenine nucleotide translocator in neurodegenerative diseases.
L. Dmitriev (2007)
Glutathione ester protects against hydroxynonenal-induced loss of auditory hair cells
Jose W. Ruiz (2006)
Treatment of the budding yeast Saccharomyces cerevisiae with the lipid peroxidation product 4-HNE provokes a temporary cell cycle arrest in G1 phase.
W. Wonisch (1998)
An Orally Active Catalytic Metalloporphyrin Protects against 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine Neurotoxicity In Vivo
Li-ping Liang (2007)
Transfection of HepG2 cells with hGSTA4 provides protection against 4-hydroxynonenal-mediated oxidative injury.
E. Gallagher (2007)
Regulation of constitutive neutrophil apoptosis by the α,β-unsaturated aldehydes acrolein and 4-hydroxynonenal
E. Finkelstein (2005)
Cytotoxic effects of various stressors on PC12 cells: involvement of oxidative stress and effect of antioxidants.
R. Piga (2007)
The lipid peroxidation product 4-hydroxy-2,3-nonenal inhibits constitutive and inducible activity of nuclear factor kappa B in neurons.
S. Camandola (2000)
Dopamine D2 receptor gene expression and binding sites in adrenal medulla and pheochromocytoma.
C. Pupilli (1994)
Interaction of Escherichia coli heat-stable enterotoxin B with rat intestinal epithelial cells and membrane lipids.
K. Chao (1999)
Toxicity and detoxification of lipid-derived aldehydes in cultured retinal pigmented epithelial cells.
S. Choudhary (2005)
Role of 4-hydroxynonenal and its metabolites in signaling
S. Dwivedi (2007)
The role of dopamine in manganese-induced oxidative injury in rat pheochromocytoma cells
K. Seth (2002)
Selective cleavage of thioether linkage in proteins modified with 4-hydroxynonenal.
K. Uchida (1992)
Effect of NaCl on the Lipid Peroxidation-Derived Aldehyde, 4-Hydroxy-2-nonenal, Formation in Boiled Pork
T. Sakai (2006)
Involvement of PI3K/PKG/ERK1/2 signaling pathways in cortical neurons to trigger protection by cotreatment of acetyl-L-carnitine and alpha-lipoic acid against HNE-mediated oxidative stress and neurotoxicity: implications for Alzheimer's disease.
H. Abdul (2007)
In vitro cytotoxicity evaluation of plastic biomedical devices
A. Pant (2001)

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Cytotoxicity assessments of Portulaca oleracea and Petroselinum sativum seed extracts on human hepatocellular carcinoma cells (HepG2).
N. N. Farshori (2014)
Coumarin centered copper(II) complex with appended-imidazole as cancer chemotherapeutic agents against lung cancer: molecular insight via DFT-based vibrational analysis
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Cytotoxicity of Nigella sativa seed oil and extract against human lung cancer cell line.
E. Al-sheddi (2014)
4-Hydroxynonenal induces mitochondrial-mediated apoptosis and oxidative stress in SH-SY5Y human neuronal cells.
S. Abarikwu (2012)
4-Oxo-2-nonenal as a pro-oxidant during the autoxidation of methyl linoleate in bulk phase
F. Saito (2011)
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E. Al-sheddi (2015)
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M. Khan (2016)
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M. A. Siddiqui (2013)
In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell lines.
M. Al-Oqail (2013)
Caspase cascade regulated mitochondria mediated apoptosis in monocrotophos exposed PC12 cells.
M. P. Kashyap (2010)
Assessment of prevalence of hydatidosis in slaughtered Sawakny sheep in Riyadh city, Saudi Arabia
Esam Almalki (2017)
Kolaviron protects apoptotic cell death in PC12 cells exposed to Atrazine
S. Abarikwu (2011)
Protective potential of 17β-estradiol against co-exposure of 4-hydroxynonenal and 6-hydroxydopamine in PC12 cells
M. A. Siddiqui (2011)
The Role of Mitochondrial Aldehyde Dehydrogenase 2 (ALDH2) in Neuropathology and Neurodegeneration.
C. Chen (2016)
Distribution of 4-Hydroxynonenal-Protein Conjugates as a Marker of Lipid Peroxidation and Parameter of Malignancy in Astrocytic and Ependymal Tumors of the Brain
G. Juric-Sekhar (2009)
Copper(ii) l/d-valine-(1,10-phen) complexes target human telomeric G-quadruplex motifs and promote site-specific DNA cleavage and cellular cytotoxicity.
F. Arjmand (2020)
Nucleation temperature-controlled synthesis and in vitro toxicity evaluation of L-cysteine-capped Mn:ZnS quantum dots for intracellular imaging.
V. Pandey (2016)
Association of dopamine DA-D2 receptor in rotenone-induced cytotoxicity in PC12 cells
M. Siddiqui (2010)
Effect of Trans-resveratrol on Rotenone-induced Cytotoxicity in Human Breast Adenocarcinoma Cells
M. A. Siddiqui (2011)
Titanium Dioxide Nanoparticles As Guardian against Environmental Carcinogen Benzo[alpha]Pyrene
Anupam Dhasmana (2014)
Cyto-genotoxicity Assessment of Potential Anti-tubercular Drug Candidate Molecule-trans-cyclohexane-1, 4-diamine Derivative-9u in Human Lung Epithelial Cells A549
E. Kapoor (2014)
Petroselinum sativum protects HepG2 cells from cytotoxicity and oxidative stress induced by hydrogen peroxide
M. Al-Oqail (2020)
Synthesis, characterization of α-amino acid Schiff base derived Ru/Pt complexes: Induces cytotoxicity in HepG2 cell via protein binding and ROS generation.
A. Alsalme (2016)
In Vitro Cytotoxicity of Mesoporous SiO2@Eu(OH)3 Core-Shell Nanospheres in MCF-7
M. Atif (2016)
Copper(II) complexes as potential anticancer and Nonsteroidal anti-inflammatory agents: In vitro and in vivo studies
A. Hussain (2019)
Evaluation of cytotoxicity and oxidative stress induced by alcoholic extract and oil of Lepidium Sativum seeds in human liver cell line HepG2
E. Al-sheddi (2013)
ZnO Q-dots as a potent therapeutic nanomedicine for in vitro cytotoxicity evaluation of mouth KB44, breast MCF7, colon HT29 and HeLa cancer cell lines, mouse ear swelling tests in vivo and its side effects using the animal model
Zahra Fakhroueian (2018)
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S. Goel (2019)
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