← Back to Search
Oxidative Stress-dependent Toxicity Of Silver Nanoparticles In Human Hepatoma Cells.
Soohee Kim, J. Choi, Jinhee Choi, K. Chung, Kwangsik Park, Jongheop Yi, Doug-Young Ryu
Published 2009 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
Cytotoxicity induced by silver nanoparticles (AgNPs) and the role that oxidative stress plays in this process were demonstrated in human hepatoma cells. Toxicity induced by silver (Ag(+)) ions was studied in parallel using AgNO(3) as the Ag(+) ion source. Using cation exchange treatment, we confirmed that the AgNP solution contained a negligible amount of free Ag(+) ions. Metal-responsive metallothionein 1b (MT1b) mRNA expression was not induced in AgNP-treated cells, while it was induced in AgNO(3)-treated cells. These results indicate that AgNP-treated cells have limited exposure to Ag(+) ions, despite the potential release of Ag(+) ions from AgNPs in cell culture. AgNPs agglomerated in the cytoplasm and nuclei of treated cells, and induced intracellular oxidative stress. AgNPs exhibited cytotoxicity with a potency comparable to that of Ag(+) ions in in vitro cytotoxicity assays. However, the toxicity of AgNPs was prevented by use of the antioxidant N-acetylcysteine, and AgNP-induced DNA damage was also prevented by N-acetylcysteine. AgNO(3) treatment induced oxidative stress-related glutathione peroxidase 1 (GPx1) and catalase expression to a greater extent than AgNP exposure, but treatment with AgNO(3) and AgNPs induced comparable superoxide dismutase 1 (SOD1) expression levels. Our findings suggest that AgNP cytotoxicity is primarily the result of oxidative stress and is independent of the toxicity of Ag(+) ions.
This paper references
In vitro cytotoxicity of nanoparticles in mammalian germline stem cells.
L. Braydich-Stolle (2005)
Copper Sorption from Aqueous Solutions by Plasma Modified Polyacrylonitrile Beads
A. Simeonova (2006)
Phospholipid lung surfactant and nanoparticle surface toxicity: Lessons from diesel soots and silicate dusts
W. Wallace (2006)
Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria.
I. Sondi (2004)
Assessment of the Alamar Blue assay for cellular growth and viability in vitro.
G. R. Nakayama (1997)
Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria.
E. T. Hwang (2008)
Cytotoxicity of titanium dioxide nanoparticles in mouse fibroblast cells.
Cheng-Yu Jin (2008)
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
Toxic Potential of Materials at the Nanolevel
A. Nel (2006)
The role of Acticoat with nanocrystalline silver in the management of burns.
K. Dunn (2004)
Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses
K. Rothkamm (2003)
Silver-coated dressing acticoat caused raised liver enzymes and argyria-like symptoms in burn patient.
M. Trop (2006)
Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species.
C. Carlson (2008)
Characteristics of γ-H2AX foci at DNA double-strand breaks sites
D. Pilch (2003)
Insuffisance rénale aiguë après application topique de sulfadiazine argentique
G. Chaby (2005)
Evaluation of cytotoxicity in cultured cells by enzyme leakage
D. B. Mitchell (1980)
Measurement of Intracellular Fluorescence of Human Monocytes Relative to Oxidative Metabolism
J. P. Robinson (1988)
Toxicity of silver nanoparticles to Chlamydomonas reinhardtii.
E. Navarro (2008)
Toxicity of silver nanoparticles in zebrafish models.
P. Asharani (2008)
Assessment of Metal Nanoparticle Agglomeration, Uptake, and Interaction Using High-Illuminating System
Jeanne E Skebo (2007)
Cellular Toxicity of Various Inhalable Metal Nanoparticles on Human Alveolar Epithelial Cells
Seo-Young Park (2007)
Removal of heavy metals from aqueous solution by chelating resin in a multistage adsorption process.
S. H. Lin (2000)
Minimal In Vitro Antimicrobial Efficacy and Ocular Cell Toxicity from Silver Nanoparticles
Colleen M Santoro (2007)
Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm.
T. Xia (2006)
Structure and tissue-specific expression of the human metallothionein IB gene.
A. Heguy (1986)
Exposure of engineered nanoparticles to human lung epithelial cells: influence of chemical composition and catalytic activity on oxidative stress.
Ludwig K. Limbach (2007)
Biomaterials to prevent nosocomial infections: is silver the gold standard?
D. Stickler (2000)
The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells.
S. Al-Nasiry (2007)
Airborne Nanostructured Particles and Occupational Health
A. Maynard (2005)
Argyria from excessive use of topical silver sulphadiazine
ChristopherM. E. Rowland Payne (1992)
The interaction of manganese nanoparticles with PC-12 cells induces dopamine depletion.
S. Hussain (2006)
Kinetic aspects of the silver ion release from antimicrobial polyamide/silver nanocomposites
C. Damm (2008)
Cellular responses induced by silver nanoparticles: In vitro studies.
S. Arora (2008)
In vivo molecular probing of cellular compartments with gold nanoparticles and nanoaggregates.
J. Kneipp (2006)
Cytotoxic effects of aggregated nanomaterials.
K. Soto (2007)
A critical assessment of the use of microculture tetrazolium assays to measure cell growth and function.
N. J. Marshall (1995)
Copper oxide nanoparticles are highly toxic: a comparison between metal oxide nanoparticles and carbon nanotubes.
H. Karlsson (2008)
In vitro analysis of a nanocrystalline silver-coated surgical mesh.
M. Cohen (2007)
Metallothionein induction in human peripheral blood lymphocytes by heavy metals.
H. Yamada (1991)
In vitro investigation of oxide nanoparticle and carbon nanotube toxicity and intracellular accumulation in A549 human pneumocytes.
A. Simon-Deckers (2008)
The potential environmental impact of engineered nanomaterials
V. Colvin (2003)
In vitro toxicity of nanoparticles in BRL 3A rat liver cells.
S. Hussain (2005)
This paper is referenced by
Cytotoxicity of Bacteriostatic Reduced Graphene Oxide-Based Copper Oxide Nanocomposites
X. Xu (2019)
Analytical methods for nano-bio interface interactions
J. Wang (2016)
Tissue Reaction and Biocompatibility of Implanted Mineral Trioxide Aggregate with Silver Nanoparticles in a Rat Model
V. Zand (2016)
Proteomics study of silver nanoparticles on Caco-2 cells
S. Gioria (2018)
Silver Nanoparticles for the Therapy of Tuberculosis
Alexandru-Flaviu Tǎbǎran (2020)
Study of the nucleation and growth of antibiotic labeled Au NPs and blue luminescent Au8 quantum clusters for Hg(2+) ion sensing, cellular imaging and antibacterial applications.
Puneet Khandelwal (2015)
A Rapid and Sensitive Colorimetric Sensor for Detection of Silver Ions Based on the Non-aggregation of Gold Nanoparticles in the Presence of Ascorbic Acid
Arumugam Selva Sharma (2018)
Evaluation of enhanced darkfield microscopy and hyperspectral analysis to analyse the fate of silver nanoparticles in wastewaters
Trevor Théoret (2017)
Biological testing of the chemically synthesized silver nano-particles for nitrate, chloride, potassium and sodium contents, and some physiological and biochemical characteristics of tomato plants
Saeed Karami Mehrian (2016)
In vitro toxicity assessment of silver nanoparticles in the presence of phenolic compounds – preventive agents against the harmful effect?
A. Martirosyan (2014)
Silver nanoparticles induce apoptotic cell death in cultured cerebral cortical neurons
S. Kim (2014)
Cytotoxicity and cellular uptake of tri-block copolymer nanoparticles with different size and surface characteristics
Sourav Bhattacharjee (2012)
Silver Nanoparticles: Synthetic Routes, In Vitro Toxicity and Theranostic Applications for Cancer Disease
Valeria de Matteis (2018)
Silver nanoparticles promote osteogenic differentiation of human urine-derived stem cells at noncytotoxic concentrations
H. Qin (2014)
The effects of silver nanoparticles on antimicrobial activity of ProRoot mineral trioxide aggregate (MTA) and calcium enriched mixture (CEM)
N. Jonaidi-Jafari (2016)
Induction of 8-hydroxydeoxyguanosine and ultrastructure alterations by silver nanoparticles attributing to placental transfer in pregnant rats and fetuses
EI Salim (2019)
Effect of Neonatal Exposure to Poly(Ethylene Glycol)-block-Poly(Lactic Acid) Nanoparticles on Oxidative State in Infantile and Adult Female Rats
M. Dvořáková (2017)
Elucidating the genetic basis for Escherichia coli defense against silver toxicity using mutant arrays.
Zongming Xiu (2014)
Influence of pH on the transport of silver nanoparticles in saturated porous media: laboratory experiments and modeling
Jason R Flory (2013)
Interaction of nanosilver particles with human lymphocyte cells
A. Zhornik (2015)
Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential
M. Stevanović (2012)
Size-Dependent Cytotoxicity and Genotoxicity of Silver Nanoparticles in Cochlear Cells In Vitro
M. Perde-Schrepler (2019)
Sonochemically-Produced Metal-Containing Polydopamine Nanoparticles and Their Antibacterial and Antibiofilm Activity.
Gil Yeroslavsky (2016)
Apoptotic effect of silver nanoparticles in rat
I. Ashour (2017)
THE PROTECTIVE ROLE OF TANNIC ACID AGAINST POSSIBLE HEPATO-NEPHROTOXICITY INDUCED BY SILVER NANOPARTICLES ON MALE RATS
Israa F. Mosa (2019)
Silver Nanocoatings for Reducing the Exogenous Microbial Colonization of Wound Dressings
M. Rădulescu (2016)
Evaluation of Colloidal Stability and Ecotoxicity of Metal-based Nanoparticles in the Aquatic and Terrestrial Systems
L. Pokhrel (2013)
Biogenic silver nanoparticles based on trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity
Mariana Guilger (2017)
Immunomodulatory effect of gelatin-coated silver nanoparticles in mice: Ultrastructural evaluation
O. Ahmed (2016)
Phytotoxicity of silver nanoparticles to cucumber (Cucumis sativus) and wheat (Triticum aestivum)
Di Cui (2014)
Bio-distribution and Toxicity of Noble Metal Nanoparticles in Humans
I. Gupta (2017)
C. elegans-on-a-chip for in situ and in vivo Ag nanoparticles’ uptake and toxicity assay
J. Kim (2017)See more