Online citations, reference lists, and bibliographies.
← Back to Search

Toxic Response Of Nickel Nanoparticles In Human Lung Epithelial A549 Cells.

M. Ahamed
Published 2011 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Nickel nanoparticle (Ni NP) is increasingly used in modern industries such as catalysts, sensors and electronic applications. Due to wide-spread industrial applications the inhalation is the primary source of exposure to Ni NPs. However, data demonstrating the effect of Ni NPs on the pulmonary system remain scarce. The present study was designed to examine the toxic effect of human lung epithelial A549 cells treated with well characterized Ni NPs at the concentrations of 0, 1, 2, 5, 10 and 25 μg/ml for 24 and 48 h. Mitochondrial function (MTT assay), membrane leakage of lactate dehydrogenase (LDH assay), reduced glutathione (GSH), reactive oxygen species (ROS), membrane lipid peroxidation (LPO) and caspase-3 activity were assessed as toxicity end points. Results showed that Ni NPs reduced mitochondrial function and induced the leakage of LDH in dose and time-dependent manner. Ni NPs were also found to induce oxidative stress in dose and time-dependent manner indicated by depletion of GSH and induction of ROS and LPO. Further, activity of caspase-3 enzyme, marker of apoptosis was significantly higher in treated cells with time and Ni NPs dosage. The results exhibited significant toxicity of Ni NPs in human lung epithelial A549 cells which is likely to be mediated through oxidative stress. This study warrants more careful assessment of Ni NPs before their industrial applications.
This paper references
10.3181/00379727-90-21985
Lactic Dehydrogenase Activity in Blood.∗
F. Wróblewski (1955)
10.1016/0003-9861(59)90090-6
Tissue sulfhydryl groups.
G. Ellman (1959)
10.1016/0003-2697(79)90738-3
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.
H. Ohkawa (1979)
10.1016/0022-1759(83)90303-4
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
10.1016/0003-2697(85)90442-7
Measurement of protein using bicinchoninic acid.
P. Smith (1985)
Molecular properties and clinical applications
A Meister (1989)
Molecular properties and clinical applications. In: Glutathione Centennial
A. Meister (1989)
[Oxidative stress and apoptosis].
J. Mathieu (1996)
10.1016/S0378-4274(98)80220-8
Nickel-induced oxidative stress, disruption of mitochondrial integrity and cytotoxicity in human lung fetal fibroblasts (MRC-5 cells)
Zhixiong Zhuang (1998)
10.1016/S0891-5849(99)00107-0
Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader.
Hongshun Wang (1999)
Quantifying cellular oxidative stress by dichlorofluorescin assay using microplate reader. Free Radic
H Wang (1999)
kidney cells. Toxicol
J. A. Joseph (1999)
10.1016/S0928-4680(00)00053-5
Oxidative stress and apoptosis.
Kannan (2000)
10.1016/S0887-2333(03)00138-3
Evaluation of oxidative damage and inhibition of DNA repair in an in vitro study of nickel exposure.
D. Cavallo (2003)
10.1016/S0041-008X(03)00086-3
Nickel induces oxidative stress and genotoxicity in human lymphocytes.
Chang-Yu Chen (2003)
10.1539/joh.45.23
Comparative Toxicity of Standard Nickel and Ultrafine Nickel in Lung after Intratracheal Instillation
Q. Zhang (2003)
10.1378/chest.54.6.527
Nickel carcinogenesis.
K. Kasprzak (2003)
10.1289/ehp.7021
Manufactured Nanomaterials (Fullerenes, C60) Induce Oxidative Stress in the Brain of Juvenile Largemouth Bass
E. Oberdörster (2004)
Manufactured nanomaterials (fullerenes
E. Oberdorster (2004)
10.1016/J.YRTPH.2005.06.014
Assessment of respiratory carcinogenicity associated with exposure to metallic nickel: a review.
Donna J Sivulka (2005)
10.1289/ehp.7339
Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles
G. Oberdörster (2005)
10.1016/J.MRGENTOX.2005.06.001
Nickel compound-induced DNA single-strand breaks in chromosomal and nuclear chromatin in human blood lymphocytes in vitro: role of oxidative stress and intracellular calcium.
Prosper M'bemba-meka (2005)
10.1016/J.TIV.2005.06.034
In vitro toxicity of nanoparticles in BRL 3A rat liver cells.
S. Hussain (2005)
10.1074/jbc.M413344200
Novel Role for Amphiregulin in Protection from Liver Injury*
C. Berasain (2005)
Assessment of respiratory carcinogenicity
Toxicol. Lett (2005)
Nanotoxicology: an emerging
Perspect (2005)
10.1016/J.SCITOTENV.2006.04.007
Role of oxidative stress, mitochondrial membrane potential, and calcium homeostasis in nickel subsulfide-induced human lymphocyte death in vitro.
Prosper M'bemba-meka (2006)
10.1016/J.PLANTSCI.2005.08.014
Involvement of plasma-membrane NADPH oxidase in nickel-induced oxidative stress in roots of wheat seedlings
Fushun Hao (2006)
10.1126/SCIENCE.1114397
Toxic Potential of Materials at the Nanolevel
A. Nel (2006)
Nanotoxicology: signs of stress
V. Stone (2006)
10.1002/JPS.20874
Oxidative stress and apoptosis: impact on cancer therapy.
T. Ozben (2007)
10.1016/J.CCA.2007.04.024
Low level lead exposure and oxidative stress: current opinions.
M. Ahamed (2007)
10.1007/springerreference_224261
[Oxidative stress].
J. Haleng (2007)
thiobarbituric acid reaction
E. J. Park (2007)
10.1016/j.toxlet.2008.04.015
The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells.
Yi-Hong Hsin (2008)
10.1166/JNN.2008.311
In vitro cellular uptake and cytotoxic effect of functionalized nickel nanoparticles on leukemia cancer cells.
Dadong Guo (2008)
10.1021/nn800511k
Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties.
T. Xia (2008)
10.1016/j.taap.2008.09.015
DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells.
M. Ahamed (2008)
10.1016/j.jinorgbio.2008.12.017
Toxicological effects of inorganic nanoparticles on human lung cancer A549 cells.
S. Choi (2009)
10.1021/ES9010543
Toxicity and developmental defects of different sizes and shape nickel nanoparticles in zebrafish.
Cristina Ispas (2009)
10.1186/1477-3155-7-2
Metallic nickel nano- and fine particles induce JB6 cell apoptosis through a caspase-8/AIF mediated cytochrome c-independent pathway
J. Zhao (2009)
10.1016/J.TOXLET.2009.01.008
DNA damaging potential of zinc oxide nanoparticles in human epidermal cells.
V. Sharma (2009)
10.1016/j.tiv.2009.04.009
Oxidative stress contributes to silica nanoparticle-induced cytotoxicity in human embryonic kidney cells.
F. Wang (2009)
10.1021/nn800596w
Cytotoxicity and genotoxicity of silver nanoparticles in human cells.
P V AshaRani (2009)
Metallic nickel nanoand fine particles induce JB 6 cell apoptosis through a caspase8 / AIF mediated cytochrome cindependent pathway
Z. X. Zhuang (2009)
tracheal instillation
L. Bowman (2009)
10.1016/j.tiv.2009.12.001
Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism.
E. Park (2010)
10.1007/S11051-009-9740-9
Toxicity of zinc oxide nanoparticles to zebrafish embryo: a physicochemical study of toxicity mechanism
W. Bai (2010)
10.1016/j.bbrc.2010.04.156
Genotoxic potential of copper oxide nanoparticles in human lung epithelial cells.
M. Ahamed (2010)
10.1016/j.aquatox.2009.11.016
Silver nanospheres are cytotoxic and genotoxic to fish cells.
J. P. Wise (2010)
10.1016/j.cca.2010.08.016
Silver nanoparticle applications and human health.
M. Ahamed (2010)
10.1016/j.taap.2010.04.012
Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in human colon carcinoma cells.
B. De Berardis (2010)
10.1016/S1001-0742(09)60137-6
Nickel accumulation and its effect on biomass, protein content and antioxidative enzymes in roots and leaves of watercress (Nasturtium officinale R. Br.).
F. Duman (2010)
10.1016/j.taap.2009.10.016
Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster.
M. Ahamed (2010)
10.1016/j.tox.2010.07.010
Nanotoxicity of pure silica mediated through oxidant generation rather than glutathione depletion in human lung epithelial cells.
M. Akhtar (2010)
10.1016/j.tiv.2010.03.002
The primary role of iron-mediated lipid peroxidation in the differential cytotoxicity caused by two varieties of talc nanoparticles on A549 cells and lipid peroxidation inhibitory effect exerted by ascorbic acid.
M. Akhtar (2010)
10.1002/ajim.20855
Pulmonary and systemic toxicity following exposure to nickel nanoparticles.
J. I. Phillips (2010)
Silver nanospheres are cytotoxic
J. P. 612–616. Wise (2010)
cytotoxicity by a Trojan-horse type mechanism. Toxicol
J.I (2010)



This paper is referenced by
10.1016/J.MATPR.2021.04.403
Ovalbumin mediated eco-friendly synthesis of silver oxide nanoparticles and their antibacterial and antifungal studies
Indresh Kumar (2021)
10.1021/ACS.IECR.0C06203
Biomimetic Catalysis of CO2 Hydration: A Materials Perspective
Manju Verma (2021)
10.1016/J.MATPR.2021.03.735
Environmental benign synthesis and characterization of nickel oxide nanoparticles using chicken egg white as template and evaluations of their antibacterial/antifungal activities
Indresh Kumar (2021)
10.2174/1871530320666200122155804
Mechanisms of Nickel-Induced Cell Damage in Allergic Contact Dermatitis and Nutritional Intervention Strategies.
D. Filatova (2020)
10.1016/j.dyepig.2019.108092
A dual-responsive anthrapyridone-triazole-based probe for selective detection of Ni2+ and Cu2+: A mimetic system for molecular logic gates based on color change
Ashwani Kumar (2020)
10.3390/nano10091816
Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape
Laura Rueda-Gensini (2020)
10.4274/tjps.galenos.2019.76376
Cytotoxic, Genotoxic, and Apoptotic Effects of Nickel Oxide Nanoparticles in Intestinal Epithelial Cells.
M. Abudayyak (2020)
10.1016/j.chemosphere.2020.126913
Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles.
Yongya Wu (2020)
10.1007/s10653-019-00491-4
Advance on toxicity of metal nickel nanoparticles
Yongya Wu (2020)
10.1007/978-981-15-5511-4_23
Toxicological Study of Nanoparticles: An Attempt to Relate Physicochemical Characters with Toxicity
A. Seenivasan (2020)
10.1002/adma.202001114
Biocompatible Magnetic Micro- and Nanodevices: Fabrication of FePt Nanopropellers and Cell Transfection.
V. Kadiri (2020)
10.2174/1871530320666200214123118
Pathogenic Mechanisms and Therapeutic Implication in Nickel-induced Cell Damage.
A. Salimi (2020)
10.1039/C9EN00628A
Insights into short- and long-term effects of loading nickel nanoparticles on anaerobic digestion with flocculent sludge
Chuan-Shu He (2019)
10.1016/J.APSUSC.2019.03.255
Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells
Chekuri Poornavaishnavi (2019)
10.1016/j.envpol.2018.10.123
High-throughput transcriptomics: Insights into the pathways involved in (nano) nickel toxicity in a key invertebrate test species.
Susana I. L. Gomes (2019)
10.1007/978-3-030-12461-8_11
Nanocarriers and immune cells
Lorna Moll (2019)
10.1039/C9EN00190E
Progressive stress response of the anaerobic granular sludge to nickel nanoparticles: experimental investigations and mathematic modelling
Chuan-Shu He (2019)
10.1016/j.ecoenv.2019.109760
Biochemical and behavioral responses of zebrafish embryos to magnetic graphene/nickel nanocomposites.
A. R. Almeida (2019)
10.2131/jts.44.737
A comparative study of nickel nanoparticle and ionic nickel toxicities in zebrafish: histopathological changes and oxidative stress.
Shohei Yokota (2019)
10.1088/1361-6528/ab0ed0
Protein corona formation and its constitutional changes on magnetic nanoparticles in serum featuring a polydehydroalanine coating: effects of charge and incubation conditions.
C. Gräfe (2019)
Human lung cell responses caused by roadside particle types
X. Jia (2019)
AS A POTENTIAL ANTIMICROBIAL AND ANTICANCER DRUG
Preetha Bhadra (2019)
10.1016/j.chemosphere.2018.11.128
Mechanisms underlying nickel nanoparticle induced reproductive toxicity and chemo-protective effects of vitamin C in male rats.
L. Kong (2019)
10.5897/SRE2019.6604
Nanomedicines: Challenges and perspectives for future nanotechnology in the healthcare system
Ajazuddin (2019)
10.1016/j.toxrep.2018.03.012
NiO nanoparticles induce cytotoxicity mediated through ROS generation and impairing the antioxidant defense in the human lung epithelial cells (A549): Preventive effect of Pistacia lentiscus essential oil
K. Mohamed (2018)
10.1002/jat.3557
Graphene oxide induces cytotoxicity and oxidative stress in bluegill sunfish cells
K. Srikanth (2018)
10.1016/J.JALLCOM.2018.04.093
Burgeoning tool of biomedical applications - Superparamagnetic nanoparticles
L. Khanna (2018)
10.1155/2018/7278036
Genotoxicity of Copper and Nickel Nanoparticles in Somatic Cells of Drosophila melanogaster
Erico R. Carmona (2018)
10.3390/ma11112081
Cationic Substitutions in Hydroxyapatite: Current Status of the Derived Biofunctional Effects and Their In Vitro Interrogation Methods
T. Tite (2018)
10.1002/jat.3505
Assessment of genotoxicity and biodistribution of nano‐ and micron‐sized yttrium oxide in rats after acute oral treatment
Archana Panyala (2017)
10.1093/mutage/gex007
Genotoxicity study of nickel oxide nanoparticles in female Wistar rats after acute oral exposure
Naresh Dumala (2017)
10.1002/jat.3485
Iron oxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in lymphocytes
Usha Singh Gaharwar (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar