← Back to Search
Prediction, Mapping And Validation Of Tick Glutathione S-transferase B-cell Epitopes.
Charles Ndawula, Marina Amaral Xavier, B. Villavicencio, Fernanda Cortez Lopes, M. Juliano, L. F. Parizi, H. Verli, I. da Silva Vaz, R. Ligabue-Braun
Published 2020 · Medicine, Biology
Save to my Library
Download via 🐼 PaperPanda Download via oaDOI Download via OAB Download via LibKey Download via Google Google ScholarAnalyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
In search of ways to address the increasing incidence of global acaricide resistance, tick control through vaccination is regarded as a sustainable alternative approach. Recently, a novel cocktail antigen tick-vaccine was developed based on the recombinant glutathione S-transferase (rGST) anti-sera cross-reaction to glutathione S-transferases of Rhipicephalus appendiculatus (GST-Ra), Amblyomma variegatum (GST-Av), Haemaphysalis longicornis (GST-Hl), Rhipicephalus decoloratus (GST-Rd) and Rhipicephalus microplus (GST-Rm). Therefore, the current study aimed to predict the shared B-cell epitopes within the GST sequences of these tick species. Prediction of B-cell epitopes and proteasomal cleavage sites were performed using immunoinformatics algorithms. The conserved epitopes predicted within the sequences were mapped on the homodimers of the respective tick GSTs, and the corresponding peptides were independently used for rabbit immunization experiments. Based on the dot blot assay, the immunogenicity of the peptides and their potential to be recognized by corresponding rGST anti-sera raised by rabbit immunization in a previous work were investigated. This study revealed that the predicted conserved B-cell epitopes within the five tick GST sequences were localized on the surface of the respective GST homodimers. The epitopes of GST-Ra, GST-Rd, GST-Av, and GST-Hl were also shown to contain a seven residue-long peptide sequence with no proteasomal cleavage sites, whereas proteasomal digestion of GST-Rm was predicted to yield a 4-residue fragment. Given that a few proteasomal cleavage sites were found within the conserved epitope sequences of the four GSTs, the sequences could also contain a T-cell epitope. Finally, the peptide and rGST anti-sera reacted against the corresponding peptide, confirming their immunogenicity. These data support the claim that the rGSTs, used in the previous study, contain conserved B-cell epitopes, which elucidates why the rGST anti-sera cross-reacted to non-homologous tick GSTs. Taken together, the data suggest that the B-cell epitopes predicted in this study could be useful for constituting epitope-based GST tick vaccines.
This paper references
Antigenic competition and genetic control of the immune response. A hypothesis for intramolecular competition.
M. Taussig (1973)
The antigenic structure of proteins: a reappraisal.
D. Benjamin (1984)
Continuous and discontinuous protein antigenic determinants
D. Barlow (1986)
Cloning and expression of a protective antigen from the cattle tick Boophilus microplus.
K. Rand (1989)
Basic local alignment search tool.
S. Altschul (1990)
Variation in the expression of Mu-class glutathione S-transferase isoenzymes from human skeletal muscle. Evidence for the existence of heterodimers.
A. Hussey (1991)
Assessment of protein models with three-dimensional profiles
R. Lüthy (1992)
The three-dimensional structure of a glutathione S-transferase from the mu gene class. Structural analysis of the binary complex of isoenzyme 3-3 and glutathione at 2.2-A resolution.
X. Ji (1992)
Human Mu-class glutathione S-transferases present in liver, skeletal muscle and testicular tissue.
A. Hussey (1993)
Consensus motifs and peptide ligands of MHC class I molecules.
K. Falk (1993)
Commercialisation of a recombinant vaccine against Boophilus microplus.
P. Willadsen (1995)
The three-dimensional structure of peptide-MHC complexes.
D. Madden (1995)
Conjugation of Peptides to Carrier Proteins via Glutaraldehyde
J. M. Carter (1996)
Comparative vaccination of cattle against Boophilus microplus with recombinant antigen Bm86 alone or in combination with recombinant Bm91
P. Willadsen (1996)
Mapping Epitope Structure and Activity: From One-Dimensional Prediction to Four-Dimensional Description of Antigenic Specificity
M. V. Regenmortel (1996)
Errors in protein structures
R. Hooft (1996)
Protection against Boophilus annulatus infestations in cattle vaccinated with the B. microplus Bm86-containing vaccine Gavac. off.
H. Fragoso (1998)
Field studies and cost-effectiveness analysis of vaccination with Gavac against the cattle tick Boophilus microplus.
J. de la Fuente (1998)
Field studies and cost-effectiveness analysis of vaccination with Gavac? against the cattle tick Boophilus microplus*1
J. Delafuente (1998)
Vaccination against ticks (Boophilus spp.): the experience with the Bm86-based vaccine Gavac.
J. de la Fuente (1999)
Degradation of cell proteins and the generation of MHC class I-presented peptides.
K. Rock (1999)
Distinctive structure of the human GSTM3 gene-inverted orientation relative to the mu class glutathione transferase gene cluster.
Y. Patskovsky (1999)
Control of ticks resistant to immunization with Bm86 in cattle vaccinated with the recombinant antigen Bm95 isolated from the cattle tick, Boophilus microplus.
J. C. Garcia-Garcia (2000)
The Protein Data Bank
H. Berman (2000)
Comparative protein structure modeling of genes and genomes.
M. Martí-Renom (2000)
Immunization of cattle with synthetic peptides derived from the Boophilus microplus gut protein (Bm86).
J. Patarroyo (2002)
From immunome to vaccine: epitope mapping and vaccine design tools.
A. D. De Groot (2003)
Predicting proteasomal cleavage sites: a comparison of available methods.
Patricia Saxová (2003)
Immunoinformatics: Bioinformatic Strategies for Better Understanding of Immune Function
G. Bock (2003)
Mapping T cell epitopes by flow cytometry.
B. Hoffmeister (2003)
Sequence Variations in the Boophilus Microplus Bm86 Locus and Implications for Immunoprotection in Cattle Vaccinated with this Antigen
J. C. Garcia-Garcia (2004)
Evidence for the Utility of the Bm86 Antigen from Boophilus Microplus in Vaccination Against Other Tick Species
S. De Vos (2004)
MHC ligands and peptide motifs: first listing
H. Rammensee (2004)
Integrated control of Boophilus microplus ticks in Cuba based on vaccination with the anti-tick vaccine Gavac TM
M. R. Valle (2004)
Integrated control of Boophilus microplus ticks in Cuba based on vaccination with the anti-tick vaccine Gavac TM
M. R. Valle (2004)
Internally quenched fluorogenic protease substrates: Solid-phase synthesis and fluorescence spectroscopy of peptides containing ortho-aminobenzoyl/dinitrophenyl groups as donor-acceptor pairs
I. Hirata (2004)
A cross-reactive tick cement antigen is a candidate broad-spectrum tick vaccine.
A. Trimnell (2005)
Pcleavage: an SVM based method for prediction of constitutive proteasome and immunoproteasome cleavage sites in antigenic sequences
M. Bhasin (2005)
The role of the proteasome in generating cytotoxic T-cell epitopes: insights obtained from improved predictions of proteasomal cleavage
M. Nielsen (2005)
SCRATCH: a protein structure and structural feature prediction server
Jianlin Cheng (2005)
Improved method for predicting linear B-cell epitopes
Jens Erik Pontoppidan Larsen (2006)
Prediction of linear B-cell epitopes using amino acid pair antigenicity scale
J. Chen (2006)
Prediction of continuous B‐cell epitopes in an antigen using recurrent neural network
S. Saha (2006)
Differential expression of two glutathione S‐transferases identified from the American dog tick, Dermacentor variabilis
S. M. Dreher-Lesnick (2006)
Performance of two Bm86 antigen vaccin formulation against tick using crossbreed bovines in stall test.
R. Andreotti (2006)
Strategies for development of vaccines for control of ixodid tick species
J. de la Fuente (2006)
Exposed and concealed antigens as vaccine targets for controlling ticks and tick‐borne diseases
P. Nuttall (2006)
Two serine protease inhibitors (serpins) that induce a bovine protective immune response against Rhipicephalus appendiculatus ticks.
S. Imamura (2006)
Upcoming and future strategies of tick control : a review
S. Ghosha (2007)
A ten-year review of commercial vaccine performance for control of tick infestations on cattle
J. de la Fuente (2007)
The Aedes aegypti glutathione transferase family.
N. Lumjuan (2007)
ElliPro: a new structure-based tool for the prediction of antibody epitopes
J. Ponomarenko (2008)
Effects of anti-tick cocktail vaccine against Rhipicephalus appendiculatus.
S. Imamura (2008)
Measuring elastase, proteinase 3 and cathepsin G activities at the surface of human neutrophils with fluorescence resonance energy transfer substrates
B. Korkmaz (2008)
Antigen cocktails: valid hypothesis or unsubstantiated hope?
P. Willadsen (2008)
The known unknowns of antigen processing and presentation
J. Vyas (2008)
Predicting linear B‐cell epitopes using string kernels
Y. El-Manzalawy (2008)
Identification of conformational B-cell Epitopes in an antigen from its primary sequence
H. Ansari (2010)
Mapping protective epitopes in the tick and mosquito subolesin ortholog proteins.
C. Prudencio (2010)
Bm86 midgut protein sequence variation in South Texas cattle fever ticks
Jeanne M. Freeman (2010)
Recent advances in B-cell epitope prediction methods
Y. El-Manzalawy (2010)
In silico analysis of glutathione S-transferase supergene family revealed hitherto unreported insect specific δ- and ɛ-GSTs and mammalian specific μ-GSTs in Ixodes scapularis (Acari: Ixodidae)
B. P. Reddy (2011)
Cross immunity with Haemaphysalis longicornis glutathione S-transferase reduces an experimental Rhipicephalus (Boophilus) microplus infestation.
L. F. Parizi (2011)
Reliable B Cell Epitope Predictions: Impacts of Method Development and Improved Benchmarking
J. Kringelum (2012)
Vaccines: From Empirical Development to Rational Design
C. Rueckert (2012)
Multi-antigenic vaccine against the cattle tick Rhipicephalus (Boophilus) microplus: a field evaluation.
L. F. Parizi (2012)
J. Gershoni (2012)
Control of multiple arthropod vector infestations with subolesin/akirin vaccines.
J. A. Moreno-Cid (2013)
In silico models for B-cell epitope recognition and signaling.
H. Ansari (2013)
Subolesin/Akirin vaccines for the control of arthropod vectors and vectorborne pathogens.
J. de la Fuente (2013)
Tick vaccines and the control of tick-borne pathogens
O. Merino (2013)
Computational prediction of B cell epitopes from antigen sequences.
Jianzhao Gao (2014)
B‐cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis
L. M. Assis (2014)
Economic importance of ticks and their effective control strategies
H. V. Manjunathachar (2014)
Phylogenetic analysis of mitochondrial genome sequences indicates that the cattle tick, Rhipicephalus (Boophilus) microplus, contains a cryptic species.
Thomas D. Burger (2014)
Trade-offs in antibody repertoires to complex antigens
L. Childs (2015)
An overview of bioinformatics tools for epitope prediction: Implications on vaccine development
R. Soria-Guerra (2015)
Tick vaccines: current status and future directions
J. de la Fuente (2015)
An Introduction to B-Cell Epitope Mapping and In Silico Epitope Prediction
L. Potocnakova (2016)
Cattle tick vaccine researchers join forces in CATVAC
T. Schetters (2016)
Strategies for new and improved vaccines against ticks and tick‐borne diseases
J. Fuente (2016)
B-cell epitope mapping for the design of vaccines and effective diagnostics
Tarek Adnan Ahmad (2016)
Optimal immunization cocktails can promote induction of broadly neutralizing Abs against highly mutable pathogens
J. Shaffer (2016)
Design of the ATAQ peptide and its evaluation as an immunogen to develop a Rhipicephalus vaccine.
A. A. R. Aguirre (2016)
Functional characterization of candidate antigens of Hyalomma anatolicum and evaluation of its cross-protective efficacy against Rhipicephalus microplus.
B. Kumar (2017)
The development of veterinary vaccines: a review of traditional methods and modern biotechnology approaches
Sérgio Jorge (2017)
Issues in vaccinology: Present challenges and future directions
Dylan Sheerin (2017)
BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes
M. C. Jespersen (2017)
Fundamentals and Methods for T- and B-Cell Epitope Prediction
Jose L Sanchez-Trincado (2017)
Effect of recombinant glutathione S-transferase as vaccine antigen against Rhipicephalus appendiculatus and Rhipicephalus sanguineus infestation.
G. Sabadin (2017)
Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding
E. Hernandez (2018)
Anti-tick vaccines in the omics era.
M. R. Valle (2018)
Exploitation of reverse vaccinology and immunoinformatics as promising platform for genome-wide screening of new effective vaccine candidates against Plasmodium falciparum
Manisha Pritam (2018)
Analysis of Bm86 conserved epitopes: is a global vaccine against Cattle Tick Rhipicephalus microplus possible?
I. M. Z. Blecha (2018)
Functional Evolution of Subolesin/Akirin
Sara Artigas-Jerónimo (2018)
Advances in In-silico B-cell Epitope Prediction.
Pingping Sun (2019)
Constituting a glutathione S-transferase-cocktail vaccine against tick infestation.
Charles Ndawula (2019)
A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model
Sara Artigas-Jerónimo (2020)
This paper is referenced by
Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes
Cocktail Anti-Tick Vaccines: The Unforeseen Constraints and Approaches toward Enhanced Efficacies
Charles Ndawula (2020)