Online citations, reference lists, and bibliographies.
← Back to Search

Androgen Repression Of The Production Of A 29-kilodalton Protein And Its MRNA In The Rat Ventral Prostate.

A. Saltzman, R. Hiipakka, C. Chang, S. Liao
Published 1987 · Medicine, Biology

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The regression of the ventral prostate, after a rat is deprived of androgens by castration, is accompanied by a marked decrease in the prostate's ability to synthesize RNA and major proteins. Surprisingly, in vitro translation of prostate RNA, isolated from rats 2 days after castration, detects four proteins with Mr of approximately 29,000, 37,000, 46,000, and 49,000 whose message levels increased 4- to 12-fold compared to results from normal rats. According to cDNA dot hybridization analysis, the increase after castration in the level of the 29-kDa protein-mRNA (per unit amount of DNA) was reversed within 6 h by androgen treatment of castrated rats. In contrast, the level of a mRNA in male rat liver, which hybridized to a cloned probe for the prostate 29-kDa protein-mRNA was reduced by castration and increased by androgen treatment. During an in vitro incubation, the ventral prostates of normal rats were much less efficient than the prostates of rats castrated 2 days earlier in synthesizing a 29-kDa protein. Despite the fact that androgenic manipulation of rats induced very rapid and significant changes in the production of the 29-kDa protein and in the level of its mRNA, the cellular level of this protein in the prostate, as determined by radioimmunoassay, was maintained at near normal values throughout the 2-week experimental period. Thus, the prostate appears to have a mechanism, based on androgen repression of certain genes, to maintain the cellular levels of the 29-kDa protein and possibly other structurally or functionally important proteins during both the periods of androgen-dependent growth and the castration-induced regression. The loss of such a regulatory mechanism may result in androgen-independent abnormal prostate growth.
This paper references



This paper is referenced by
10.1210/MEND-2-12-1276
The rat androgen receptor: primary structure, autoregulation of its messenger ribonucleic acid, and immunocytochemical localization of the receptor protein.
J. Tan (1988)
10.1016/S0168-8278(96)80216-9
Inhibition of growth and induction of TGF-beta 1 in human hepatocellular carcinoma with androgen receptor by cyproterone acetate in male nude mice.
N. Nagasue (1996)
10.1016/0022-4731(89)90156-8
Mechanism of androgen action: recent observations on the domain structure of androgen receptors and the induction of EGF-receptors by androgens in prostate tumor cells.
E. Mulder (1989)
10.1007/BF00235188
Post-castration rebound of an androgen regulated prostatic gene
R. Sweetland (2004)
10.1293/TOX.14.267
Correlation between Prostatic Atrophy and Apoptosis in the Canine Spontaneous Benign Prostatic Hyperplasia (BPH) Following Chlormadinone Acetate (CMA) Administration
M. Murakoshi (2001)
10.1002/PROS.2990130203
Activation of a Ca2+−Mg2+‐dependent endonuclease as an early event in castration‐induced prostatic cell death
N. Kyprianou (1988)
10.1267/AHC.34.223
Chlormadinone Acetate(CMA) Induces Apoptosis on Canine Spontaneous Benign Prostatic Hyperplasia(BPH)
M. Murakoshi (2001)
10.1016/S0022-5347(17)39902-0
Proteins of the rat prostate. III. Effect of testosterone on protein synthesis by the ventral prostate of castrated rats.
J. Sensibar (1990)
10.1007/978-1-4612-3162-2_12
Growth Factors Affecting Normal and Malignant Prostatic Cells
J. Isaacs (1991)
10.1007/BF00048064
Active cell death in hormone-dependent tissues
M. Tenniswood (2004)
10.1007/978-3-642-79437-7_6
Regulation of apoptosis via steroid receptors.
M. Iwata (1995)
10.1111/J.1365-2605.1989.TB01307.X
Developmental pattern of androgen-regulated proteins in seminal vesicles from the mouse.
T. Normand (1989)
10.1002/JEZ.1402720508
Photoperiod-induced changes in the proteins secreted by the male genital tract of the rodent Octodon degus.
R. Carballada (1995)
10.3390/molecules21050602
Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ
Shu-wu Xie (2016)
10.1002/JEZ.1402500213
In vitro biosynthesis and secretion of rabbit epididymal secretory proteins: regulation by androgens.
F. Regalado (1989)
10.1007/978-1-4615-3704-5_4
Therapeutic Approaches to Activating Programmed Cell Death of Androgen-Independent Prostatic Cancer Cells
N. Kyprianou (1991)
10.1507/ENDOCRJ1954.38.619
Isolation and characterization of cDNA clones for castration-induced mRNAs in the rat ventral prostate.
Y. Hoshikawa (1991)
10.1016/J.BEEM.2005.09.001
Strengths and weaknesses of in vitro assays for estrogenic and androgenic activity.
A. Soto (2006)
Programmed Cell Death during Regression of PC-82 Human Prostate Cancer following Androgen Ablation 1
N. Kyprianou (2006)
10.1002/(SICI)1097-0045(199604)28:4<239::AID-PROS5>3.0.CO;2-8
Effects of castration on plasminogen activator activities and plasminogen activator inhibitor type I in the rat ventral prostate
M. Wilson (1996)
10.1080/014850199262689
Androgen-dependent synthesis/secretion of caltrin, calcium transport inhibitor protein of mammalian seminal vesicle.
M. Novella (1999)
Prostate Cancer following Androgen Ablation Programmed Cell Death during Regression of PC-82 Human Updated
N. Kyprianou (2006)
10.1002/PROS.2990130205
Treatment with antiandrogens induces an androgen‐repressed gene in the rat ventral prostate
Jocelyne G. Léger (1988)
10.1002/AR.1092290306
Immunocytochemical localization of cathepsin D in rat ventral prostate: Evidence for castration‐induced expression of cathepsin D in basal cells
M. Wilson (1991)
10.1210/ENDO-123-2-687
Testosterone suppression of ornithine decarboxylase activity in rat Sertoli cells.
T. Swift (1988)
10.1038/cr.1992.1
A protein in rat prostatic chromatin interacting with androgen regulated gene
Y. Xu (1992)
10.1210/MEND-5-10-1381
Isolation and characterization of transcripts induced by androgen withdrawal and apoptotic cell death in the rat ventral prostate.
M. Briehl (1991)
10.1002/JCP.1041540316
Elevated glutathione S‐transferase gene expression is an early event during steroid‐induced lymphocyte apoptosis
F. A. Flomerfelt (1993)
10.1007/978-1-4615-2926-2
The Underlying Molecular, Cellular and Immunological Factors in Cancer and Aging
S. S. Yang (1993)
Relationship between prostatic atrophy and apoptosis in the canine spontaneous benign prostatic hyperplasia (BPH) following chlormadinone acetate (CMA).
M. Murakoshi (2001)
10.1074/jbc.273.22.13675
Dynamics of Protein Kinase CK2 Association with Nucleosomes in Relation to Transcriptional Activity*
C. Guo (1998)
10.1210/ENDO.130.2.1370795
Androgens up-regulate the human prostate-specific antigen messenger ribonucleic acid (mRNA), but down-regulate the prostatic acid phosphatase mRNA in the LNCaP cell line.
P. Henttu (1992)
See more
Semantic Scholar Logo Some data provided by SemanticScholar