Online citations, reference lists, and bibliographies.
← Back to Search

Detection Of Peptidases In Trypanosoma Cruzi Epimastigotes Using Chromogenic And Fluorogenic Substrates.

N. Healy, S. Greig, H. Enahoro, H. Roberts, L. Drake, E. Shaw, F. Ashall
Published 1992 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Detergent extracts of Trypanosoma cruzi epimastigotes catalysed the hydrolysis of a range of amino-acyl and peptidyl p-nitro-anilides and aminomethylcoumarins. At least three enzymes were detected that cleave Z-Phe-Arg-MCA. Two of these were optimally active at alkaline pH, the other at pH 4.0. Of the two enzymes with alkaline pH optima, one was a cysteine peptidase and was unable to cleave Bz-Arg-MCA readily, whilst the other cleaved Bz-Arg-MCA and was inhibited by diisopropyl fluorophosphate. The acidic enzyme was similar to cathespin L of other eukaryotes with respect to its pH profile, substrate-specificity and inhibitor-sensitivity. Evidence was presented that epimastigotes contain a cysteine-type dipeptidyl aminopeptidase, one or more aminopeptidases, and a serine peptidase that cleaves Boc-Ala-Ala-pNA. Digitonin solubilization of the activities from cells supports the hypothesis that the cathespin L-like enzyme and the dipeptidyl aminopeptidase are lysosomal, whilst the Bz-Arg-MCA hydrolase, the aminopeptidases and the Boc-Ala-Ala-pNA serine peptidase are cytosolic.
This paper references
10.1016/0003-9861(61)90145-X
The preparation and properties of two new chromogenic substrates of trypsin.
B. Erlanger (1961)
10.1016/S0006-291X(67)80055-X
On the size of the active site in proteases. I. Papain.
I. Schechter (1967)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1111/J.1550-7408.1977.TB01021.X
Proteolytic activites in cell extracts of Trypanosoma cruzi.
S. Itow (1977)
10.1016/0014-4894(78)90039-5
Trypanosoma cruzi: isolation and characterization of a protease.
V. Bongertz (1978)
10.1016/0014-4894(81)90075-8
Trypanosoma cruzi: isolation and characterization of a proteinase.
H. Rangel (1981)
10.1016/S0076-6879(81)80043-2
[41] Cathepsin B, cathepsin H, and cathepsin L
A. Barrett (1981)
10.1099/00221287-129-2-285
A minimal medium for the cultivation of infective Trypanosoma cruzi epimastigotes.
J. L. Ávila (1983)
10.1016/0300-9629(84)90170-1
Dynamics of oocyte development and related changes in serum estradiol-17β, yolk precursor, and lipid levels in the teleostean fish, Leptocottus armatus
V. Devlaming (1984)
10.1016/0166-6851(85)90034-9
The effect of proteolytic enzymes and protease inhibitors on the interaction Trypanosoma cruzi-fibroblasts.
M. Piras (1985)
10.1042/BJ2390633
The synthesis of peptidylfluoromethanes and their properties as inhibitors of serine proteinases and cysteine proteinases.
P. Rauber (1986)
10.1016/s0021-9258(18)67621-5
The major surface protein of Leishmania promastigotes is a protease.
R. Etges (1986)
10.1016/0003-9861(87)90129-9
Hormonal regulation of dipeptidyl-aminopeptidase I activity in cultured human fibroblasts.
M. Davis (1987)
10.1111/J.1432-1033.1987.TB13634.X
Lysosomal and non-lysosomal peptidyl hydrolases of the bloodstream forms of Trypanosoma brucei brucei.
J. Lonsdale-Eccles (1987)
A novel dipeptidyl aminopeptidase in rat brain membranes. Its isolation, purification, and characterization.
K. Hui (1988)
10.1021/BI00407A042
Mechanism-based isocoumarin inhibitors for trypsin and blood coagulation serine proteases: new anticoagulants.
C. Kam (1988)
10.1016/0014-5793(88)80600-8
Active center differences between cathepsins L and B: The S1 binding region
H. Kirschke (1988)
10.1016/0166-6851(89)90040-6
Subcellular localization of a cysteine proteinase from Trypanosoma cruzi.
E. Bontempi (1989)
10.1016/0006-291X(90)92179-4
Lysis of trypanosomes by peptidyl fluoromethyl ketones.
F. Ashall (1990)
10.1016/0304-4165(90)90091-A
Substrate specificity and inhibitor sensitivity of a trypanosomatid alkaline peptidase.
F. Ashall (1990)
10.1016/0166-6851(90)90005-7
Electrophoretic detection of Trypanosoma cruzi peptidases.
S. Greig (1990)
10.1016/0166-6851(90)90207-3
Characterisation of an alkaline peptidase of Trypanosoma cruzi and other trypanosomatids.
F. Ashall (1990)
10.1016/0167-4838(90)90166-D
Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi.
J. Cazzulo (1990)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar