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Capillary Gas Chromatographic Determination Of Polycyclic Aromatic Compounds In Vertebrate Fish Tissue.

D. Vassilaros, P. Stoker, G. Booth, M. Lee
Published 1982 · Chemistry, Medicine

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Polycyclic aromatic compound fractions were resolved from vertebrate fish tissue and analyzed by capillary column gas chromatography. The analytical procedure includes the following steps: aqueous alkaline digestion, acidification of the digestate with glacial acetic acid, extraction with methylene chloride, liquid-liquid partitioning with water and then a 10% KOH solution, adsorption chromatography on basic alumina, using hexane, benzene, and chloroform sequentially, gel permeation chromatography on BioBeads with methylene chloride, and capillary gas chromatography and gas chromatography/mass spectrometry. Examples of chromatograms of the polycyclic aromatic compound fractions from fish from polluted and pristine waterways are given. The limit of detection is less than 0.2 ppb (ng/g wet tissue), and average recovery is 72% of spiked /sup 14/C-labeled anthracene.
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