Online citations, reference lists, and bibliographies.
← Back to Search

Immunoassay With Multicomponent Protein Microarrays Fabricated By Electrospray Deposition.

N. V. Avseenko, T. Morozova, Fasoil I. Ataullakhanov, V. Morozov
Published 2002 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Two new techniques were recently developed in these laboratories for fabrication of protein microarrays: electrospray deposition of dry proteins and covalent linking of proteins from dry deposits to a dextran-grafted surface. Here we apply these techniques to simultaneously fabricate 1200 identical microarrays. Each microarray, 0.6 x 0.6 mm2 in size, consists of 28 different protein antigens and allergens deposited as spots, 30-40 microm in diameter. The ability of the microarrays to detect IgG antibodies in plasma samples from mice immunized with different sets of antigens and IgE antibodies in human plasmas was examined using ELISA. Comparison of the microarray-based ELISAs with standard ELISAs in microtiter plates established that both techniques provided identical responses in 88% of all the antibody/antigen interactions tested. Both techniques showed similar antibody detecting sensitivity defined by the maximum dilution of serum at which a reliable signal distinguishable from the background was obtained.

This paper is referenced by
A new time-resolved fluorometric microarray detection system using core–shell-type fluorescent nanosphere and its application to allergen microarray
T. Matsuya (2006)
Optimization of electrohydrodynamic writing technique to print collagen
H. Kim (2007)
Optical technologies for the read out and quality control of DNA and protein microarrays
M. Schaeferling (2006)
Functional protein microarray
Zhi Xie (2010)
Upconverting diagnostics: Multiplex assays utilizing upconversion luminescence technology
M. Ylihärsilä (2014)
Electrospray deposition of biomolecules: applications, challenges, and recommendations
Shalinee Kavadiya (2018)
Fluorescence Analysis in Microarray Technology
S. Nagl (2005)
Particle Lithography Enables Fabrication of Multicomponent Nanostructures.
Wei-feng Lin (2013)
Atomic Force Microscope Lithography on Biomimetic Surfaces
Marcus A. Kramer (2010)
Minimizing nonspecific protein adsorption in liquid crystal immunoassays by using surfactants.
Wei Zhang (2011)
In vitro responses to electrosprayed alkaline phosphatase/calcium phosphate composite coatings.
L. D. de Jonge (2009)
Force differentiation in recognition of cross-reactive antigens by magnetic beads.
T. Morozova (2008)
BioMEMS: state-of-the-art in detection, opportunities and prospects.
R. Bashir (2004)
Bioactive Paper Through Inkjet Printing
Sabina Di Risio (2010)
State of the art and recent advances in immunoanalytical systems.
C. Marquette (2006)
Protein and Chemical Microarrays—Powerful Tools for Proteomics
Qingchai Xu (2003)
Immobilization of antibodies and enzymes on 3-aminopropyltriethoxysilane-functionalized bioanalytical platforms for biosensors and diagnostics.
S. K. Vashist (2014)
Arrangement of Ferritin Molecules on a Gold Disk Array Fabricated on Highly Ordered Anodic Porous Alumina Substrate
H. Masuda (2004)
Collagen Nanofiber on Titanium or Partially Stabilized Zirconia by Electrospray Deposition
Tohru Hayakawa (2010)
Chapter 3.3 – Separation Systems
David Wild (2013)
Application of Electrosprays to Synthesis and Measurement of Nanoparticles
W. Lenggoro (2005)
Current perspectives in protein array technology
L. Kricka (2006)
Self-Limiting Electrospray Deposition for the Surface Modification of Additively-Manufactured Parts.
Dylan A Kovacevich (2020)
Strategies and Methods in Monitoring and Targeting Protein–Protein Interactions
A. Loregian (2010)
Demonstration of multi-analyte patterning using piezoelectric inkjet printing of multiple layers.
Melissa S. Hasenbank (2008)
Active bead-linked immunoassay on protein microarrays.
V. Morozov (2006)
Direct pattern formation of bacterial cells using micro-droplets generated by electrohydrodynamic forces
Ju-han Kim (2009)
13 – Detecting proteins with allergenic potential
R. Krska (2004)
Comparative and Functional Genomics Vol 3 No 4
Functional protein microarray technology
Shaohui Hu (2011)
Protein microarrays and proteomics
G. MacBeath (2002)
Poly(ethylene oxide) thin films produced by electrospray deposition: morphology control and additive effects of alcohols on nanostructure.
K. Morota (2004)
See more
Semantic Scholar Logo Some data provided by SemanticScholar