Online citations, reference lists, and bibliographies.
← Back to Search

Optical Nanosensors For Chemical Analysis Inside Single Living Cells. 2. Sensors For PH And Calcium And The Intracellular Application Of PEBBLE Sensors.

H. Clark, R. Kopelman, R. Tjalkens, M. Philbert
Published 1999 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced for intracellular measurements of pH and calcium. Five varieties of pH-sensitive sensors and three different calcium-selective sensors are presented and discussed. Each sensor combines an ion-selective fluorescent indicator and an ion-insensitive internal standard entrapped within an acrylamide polymeric matrix. Calibrations and linear ranges are presented for each sensor. The photobleaching of dyes incorporated into PEBBLEs is comparable to that of the respective free dye that is incorporated within the matrix. These PEBBLE sensors are fully reversible over many measurements. The leaching of fluorescent indicator from the polymer is less than 50% over a 48-h period (note that a typical application time is only a few hours). The PEBBLE sensors have also been applied to intracellular analysis of the calcium flux in the cytoplasm of neural cells during the mitochondrial permeability transition. Specifically, a distinct difference is noted between cells of different types (astrocyte vs neuron-derived cells) with respect to their response to the toxicant m-dinitrobenzene (DNB). Use of PEBBLE sensors permits the quantitative discrimination of subtle differences between the ability of human SY5Y neuroblastoma and C6 glioma to respond to challenge with DNB. Specifically, measurement of intracellular calcium, the precursor to cell death, has been achieved.



This paper is referenced by
10.1097/00006123-200110000-00002
2001: Things to Come
M. Apuzzo (2001)
10.1146/annurev.anchem.1.031207.112823
Nanoparticle PEBBLE sensors in live cells and in vivo.
Y. K. Lee (2009)
10.1007/978-1-4614-7061-8_5
Polymeric Food Additives
A. Akelah (2013)
10.1021/acs.analchem.5b03080
Development of an Optical Nanosensor Incorporating a pH-Sensitive Quencher Dye for Potassium Imaging.
A. Sahari (2015)
Nanobiosensors and fluorescence based biosensors: An overview
Koyeli Girigoswami (2019)
10.1002/marc.201000283
Multifunctional Poly(2-oxazoline) Nanoparticles for Biological Applications.
Kristian Kempe (2010)
10.1039/c1cc14627k
Dual colored mesoporous silica nanoparticles with pH activable rhodamine-lactam for ratiometric sensing of lysosomal acidity.
Shuqi Wu (2011)
10.1109/IEMBS.2005.1616701
Quenching Properties of a Self-Referenced Fluorescence Oxygen Nanosensor under a Wide-Field Intrinsic Optical Signal Imaging System
G. Zhang (2005)
10.1007/S00604-005-0418-4
A Novel Nano-Sensor Based on Rhodamine-β-Isothiocyanate – Doped Silica Nanoparticle for pH Measurement
F. Gao (2005)
10.15373/2249555X/JAN2014/16
Nanomedicines Targeting Cancer: Current Status and Future Prospects of the Therapeutic and Diagnostic Approaches
Foram Shukla (2011)
Integrated free radical sensor systems for investigation of cellular models of disease
S. Boulton (2012)
10.1155/2010/715295
DNA-Based Applications in Nanobiotechnology
K. Abu-Salah (2010)
10.1039/c2an35671f
Development of a pH-activatable fluorescent probe and its application for visualizing cellular pH change.
R. Huang (2012)
10.1039/C5RA19180G
Indole-based pH probe with ratiometric fluorescence behavior for intracellular imaging
Ming Nan (2015)
10.1117/12.472115
Use of steady-state fluorescence anisotropy with pebble nanosensors for chemical analysis
T. Horvath (2002)
10.1039/B501536G
Materials for fluorescence-based optical chemical sensors
O. S. Wolfbeis (2005)
10.1002/9780470185834.CH17
Clinical Applications of Micro‐ and Nanoscale Biosensors
D. G. Morrison (2007)
10.1007/S00706-009-0167-X
Design and characteristics of tin (II) optode based on immobilization of dithizone on a triacetylcellulose membrane and its application in canned foods
H. Tavallali (2009)
10.1016/S0167-7799(00)01477-3
The development of optical nanosensors for biological measurements.
B. Cullum (2000)
10.1021/ac203233q
Nanoparticles in measurement science.
F. P. Zamborini (2012)
CRITICAL ROLE OF AUTOMATION IN THE MANUFACTURE OF CELL & GENE THERAPIES
V. M. Chauhan (2018)
10.1039/c1an15046d
Two-photon nano-PEBBLE sensors: subcellular pH measurements.
A. Ray (2011)
10.1039/C4TB00446A
Cross-linked self-assembled micelle based nanosensor for intracellular pH measurements.
E. P. Kumar (2014)
10.2217/nnm.13.166
Hydrogel nanosensors for biophotonic imaging of chemical analytes.
A. Ray (2013)
10.1039/B608901A
K+-selective nanospheres: maximising response range and minimising response time.
M. J. Ruedas-Rama (2006)
10.1002/CJOC.200790068
A Novel Electrogenerated Chemiluminescence Reaction Scheme Using Core‐Shell Luminol‐Based SiO2 Nanoparticles as a Regulator and Its Analytical Application
L. Zhang (2007)
10.1039/b926231h
Arylethynyl receptors for neutral molecules and anions: emerging applications in cellular imaging.
Calden N. Carroll (2010)
10.1093/TOXSCI/KFI312
Research strategies for safety evaluation of nanomaterials, Part III: nanoscale technologies for assessing risk and improving public health.
D. Balshaw (2005)
10.3390/ECSA-2-B001
Polyaniline-Coated Polysulfone Membranes as Flexible Optical pH Sensors
Nedal Abu-Thabit (2015)
10.1039/B800276B
Biocompatible fluorescent nanoparticles for pH-sensoring.
S. Hornig (2008)
10.1007/978-3-642-15687-8_2
Smart Nano-systems for Tumour Cellular Diagnoses and Therapies
C. Francesco (2010)
10.1002/9781119441632.CH163
Calcium Phosphate Nanocomposites for Biomedical and Dental Applications: Recent Developments
A. H. Choi (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar