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An Infrared Spectroscopic Study Of The Interactions Of Carbohydrates With Dried Proteins.

J. Carpenter, J. Crowe
Published 1989 · Chemistry, Medicine

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Fourier-transform infrared spectroscopy was used to characterize the interaction of stabilizing carbohydrates with dried proteins. Freeze-drying of trehalose, lactose, and myo-inositol with lysozyme resulted in substantial alterations of the infrared spectra of the dried carbohydrates. In the fingerprint region (900-1500 cm-1), there were large shifts in the frequencies of bands, a decrease in absorbance, and a loss of band splitting. These effects mimic those of water on hydrated trehalose. Bands assigned to hydroxyl stretching modes (around 3350 cm-1) were decreased in intensity and shifted to higher frequencies in the presence of the protein. In complementary experiments, it was found that dehydration-induced shifts in the positions of amide I and amide II bands for lysozyme could be partially and fully reversed, respectively, when the protein was freeze-dried in the presence of either trehalose or lactose. In addition, the carboxylate band, which was not detectable in the protein dried without the sugar, was apparent when these sugars were present. myo-Inositol was less effective at shifting the amide bands, and the carboxylate band was not detected in the presence of this carbohydrate. Also tested was the concentration dependency of the carbohydrates' influence on the position of the amide II band for dried lysozyme. The results showed that the ability of a given concentration of a carbohydrate to shift this band back toward the position noted with the hydrated protein coincided, at least in the extreme cases, with the capacity of that same level of carbohydrate to preserve the activity of rabbit skeletal muscle phosphofructokinase during freeze-drying.(ABSTRACT TRUNCATED AT 250 WORDS)
This paper references
10.1016/0040-4020(65)80053-9
Conformation and intramolecular hydrogen bonding in glucose and xylose derivatives
A. J. Michell (1965)
10.1071/CH9681451
The control of phosphate contamination in an acid alumina process
P. Davey (1968)
10.1071/CH9681257
Spectra in the O-H stretching region of mono- and oligo-saccharides at low temperatures
A. Michell (1968)
10.1016/S0008-6215(00)82690-7
Infrared and raman spectroscopy of carbohydrates. : Part II: Normal coordinate analysis of α-D-glucose.
P. D. Vasko (1972)
10.1016/S0008-6215(00)85035-1
Study of hydrogen bonds in cyrstalline monosaccharides the spectra of monosaccharides derivatives in the middle-and far-infrared regions
G. A. Kogan (1973)
10.1016/S0008-6215(00)82465-9
Infrared and raman spectroscopy of carbohydrates : Part IV. Identification of configuration- and conformation-sensitive modes for D-glucose by normal coordinate analysis
J. J. Cael (1974)
10.1016/0005-2795(75)90122-1
Infrared spectroscopy of the water vapor sorption process of caseins.
M. Rueegg (1975)
10.1002/BIP.1979.360180512
Lysozyme film hydration events: An IR and gravimetric study
G. Careri (1979)
10.1139/V79-428
The correlation between O—H stretching frequencies and hydrogen bond distances in a crystalline sugar monohydrate
J. Umemura (1979)
10.1038/284372A0
Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences
C. M. Rubin (1980)
10.1038/284572A0
Correlation of IR spectroscopic, heat capacity, diamagnetic susceptibility and enzymatic measurements on lysozyme powder
G. Careri (1980)
10.1093/OXFORDJOURNALS.JBCHEM.A133468
Preferential hydration of bovine serum albumin in polyhydric alcohol-water mixtures.
K. Gekko (1981)
10.1016/0003-9861(81)90209-5
Interactions of proteins with solvent components in 8 M urea.
V. Prakash (1981)
10.1016/s0021-9258(19)68947-7
The stabilization of proteins by sucrose.
J. Lee (1981)
10.1088/0022-3735/15/10/028
A direct difference infrared cell for studying the hydration of protein glasses.
P. Poole (1982)
10.1021/BI00268A034
Preferential interactions of proteins with salts in concentrated solutions.
T. Arakawa (1982)
10.1021/BI00268A033
Stabilization of protein structure by sugars.
T. Arakawa (1982)
10.1016/0003-9861(83)90201-1
Preferential interactions of proteins with solvent components in aqueous amino acid solutions.
T. Arakawa (1983)
10.1016/0141-8130(83)90047-8
Hydration-induced conformational and flexibility changes in lysozyme at low water content
P. Poole (1983)
10.1016/0065-227X(83)90008-4
Water and proteins. II. The location and dynamics of water in protein systems and its relation to their stability and properties.
J. Edsall (1983)
10.1126/science.223.4637.701
Preservation of Membranes in Anhydrobiotic Organisms: The Role of Trehalose
J. Crowe (1984)
10.1016/0003-9861(84)90555-1
Infrared spectroscopic studies on interactions of water and carbohydrates with a biological membrane.
J. Crowe (1984)
10.1002/BIP.360230904
Sequential hydration of dry proteins: A direct difference IR investigation of sequence homologs lysozyme and α‐lactalbumin
P. Poole (1984)
10.1021/BI00320A005
Protein stabilization and destabilization by guanidinium salts.
T. Arakawa (1984)
10.1139/V84-258
A Raman and infrared study of crystalline D-fructose, L-sorbose, and related carbohydrates. Hydrogen bonding and sweetness
W. Szarek (1984)
10.1016/0003-9861(85)90628-9
Interaction of carbohydrates with dry dipalmitoylphosphatidylcholine.
L. Crowe (1985)
10.1016/S0006-3495(85)83932-1
The stabilization of proteins by osmolytes.
T. Arakawa (1985)
10.1007/978-3-642-70613-4_39
Molecular Basis of the Biological Compatibility of Nature’s Osmolytes
P. Low (1985)
10.1016/0003-9861(86)90755-1
Cryoprotection of phosphofructokinase with organic solutes: characterization of enhanced protection in the presence of divalent cations.
J. Carpenter (1986)
10.1042/BJ2330025
Infra-red and Raman spectroscopic studies of enzyme structure and function.
C. Wharton (1986)
10.1042/BJ2420001
Stabilization of dry phospholipid bilayers and proteins by sugars.
J. Crowe (1987)
10.1016/0304-4165(87)90133-4
Stabilization of phosphofructokinase with sugars during freeze-drying: characterization of enhanced protection in the presence of divalent cations.
J. Carpenter (1987)
10.1016/0011-2240(87)90049-6
Stabilization of phosphofructokinase during air-drying with sugars and sugar/transition metal mixtures.
J. Carpenter (1987)
10.1016/0304-4157(88)90015-9
Interactions of sugars with membranes.
J. Crowe (1988)
10.1016/0011-2240(88)90054-5
Modes of stabilization of a protein by organic solutes during desiccation
J. F. Carpenter (1988)
10.1016/0011-2240(88)90032-6
The mechanism of cryoprotection of proteins by solutes.
J. Carpenter (1988)



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10.1007/s10989-021-10303-y
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10.1016/J.MOLLIQ.2021.116656
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Nidhi Katyal (2021)
10.3390/ph14030262
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L. Giovannelli (2021)
10.1016/j.bbabio.2021.148413
Trehalose matrix effects on electron transfer in Mn-depleted protein-pigment complexes of photosystem II.
M. Mamedov (2021)
10.1016/j.addr.2021.02.017
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Rachel Yoon Kyung Chang (2021)
10.1016/J.LWT.2021.112352
Physical and oxidative stability of emulsions treated with bitter almond gum–soy protein isolate Maillard conjugates
Negar Ghaedi (2021)
10.1021/acs.biomac.1c00871
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Yiping Wang (2021)
10.1016/J.POWTEC.2021.02.031
Designing enhanced spray dried particles for inhalation: A review of the impact of excipients and processing parameters on particle properties
N. Alhajj (2021)
10.1016/j.xphs.2021.03.017
A Review of Formulations of Commercially Available Antibodies.
R. Strickley (2021)
10.1021/acsabm.1c00544
Influence of Lysine and TRITC Conjugation on the Size and Structure of Dextran Nanoconjugates with Potential for Biomolecule Delivery to Neurons
Darya Zeini (2021)
10.1080/07373937.2021.1903032
Progress in spray-drying of protein pharmaceuticals: Literature analysis of trends in formulation and process attributes
J. Pinto (2021)
10.1007/s00216-021-03315-6
Automated, flow-based chemiluminescence microarray immunoassay for the rapid multiplex detection of IgG antibodies to SARS-CoV-2 in human serum and plasma (CoVRapid CL-MIA)
Julia Klüpfel (2021)
10.1016/j.molstruc.2020.129194
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J. G. Luna-Valdez (2021)
10.1016/j.ejpb.2021.05.024
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Eduard Trenkenschuh (2021)
10.1016/j.bbapap.2021.140642
Target enzymes are stabilized by AfrLEA6 and a gain of α-helix coincides with protection by a group 3 LEA protein during incremental drying.
Blase M LeBlanc (2021)
10.3390/biom11030414
Trehalose Restrains the Fibril Load towards α-Lactalbumin Aggregation and Halts Fibrillation in a Concentration-Dependent Manner
Sania Bashir (2021)
10.3390/s20020417
Halochromic Polystyrene Nanofibers Obtained by Solution Blow Spinning for Wine pH Sensing
K. Miranda (2020)
10.1002/btm2.10159
Inhalable bacteriophage powders: Glass transition temperature and bioactivity stabilization
R. Y. Chang (2020)
10.1021/acs.jpcb.0c05245
Effect of Relaxation Times in Preservative Solution on Protein Deterioration Rate.
Lin Wei (2020)
10.1002/pat.4846
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Ryosuke Funaki (2020)
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K. Petsong (2020)
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T. Durak (2020)
10.1080/07391102.2020.1828167
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