Online citations, reference lists, and bibliographies.
← Back to Search

An Infrared Spectroscopic Study Of The Interactions Of Carbohydrates With Dried Proteins.

J. Carpenter, J. Crowe
Published 1989 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Fourier-transform infrared spectroscopy was used to characterize the interaction of stabilizing carbohydrates with dried proteins. Freeze-drying of trehalose, lactose, and myo-inositol with lysozyme resulted in substantial alterations of the infrared spectra of the dried carbohydrates. In the fingerprint region (900-1500 cm-1), there were large shifts in the frequencies of bands, a decrease in absorbance, and a loss of band splitting. These effects mimic those of water on hydrated trehalose. Bands assigned to hydroxyl stretching modes (around 3350 cm-1) were decreased in intensity and shifted to higher frequencies in the presence of the protein. In complementary experiments, it was found that dehydration-induced shifts in the positions of amide I and amide II bands for lysozyme could be partially and fully reversed, respectively, when the protein was freeze-dried in the presence of either trehalose or lactose. In addition, the carboxylate band, which was not detectable in the protein dried without the sugar, was apparent when these sugars were present. myo-Inositol was less effective at shifting the amide bands, and the carboxylate band was not detected in the presence of this carbohydrate. Also tested was the concentration dependency of the carbohydrates' influence on the position of the amide II band for dried lysozyme. The results showed that the ability of a given concentration of a carbohydrate to shift this band back toward the position noted with the hydrated protein coincided, at least in the extreme cases, with the capacity of that same level of carbohydrate to preserve the activity of rabbit skeletal muscle phosphofructokinase during freeze-drying.(ABSTRACT TRUNCATED AT 250 WORDS)
This paper references
10.1016/0003-9861(81)90209-5
Interactions of proteins with solvent components in 8 M urea.
V. Prakash (1981)
10.1126/science.223.4637.701
Preservation of Membranes in Anhydrobiotic Organisms: The Role of Trehalose
J. Crowe (1984)
10.1016/0304-4165(87)90133-4
Stabilization of phosphofructokinase with sugars during freeze-drying: characterization of enhanced protection in the presence of divalent cations.
J. Carpenter (1987)
10.1021/BI00320A005
Protein stabilization and destabilization by guanidinium salts.
T. Arakawa (1984)
10.1139/V84-258
A Raman and infrared study of crystalline D-fructose, L-sorbose, and related carbohydrates. Hydrogen bonding and sweetness
W. Szarek (1984)
10.1016/S0008-6215(00)82690-7
Infrared and raman spectroscopy of carbohydrates. : Part II: Normal coordinate analysis of α-D-glucose.
P. D. Vasko (1972)
10.1016/0040-4020(65)80053-9
Conformation and intramolecular hydrogen bonding in glucose and xylose derivatives
A. J. Michell (1965)
10.1016/0003-9861(84)90555-1
Infrared spectroscopic studies on interactions of water and carbohydrates with a biological membrane.
J. Crowe (1984)
10.1002/BIP.360230904
Sequential hydration of dry proteins: A direct difference IR investigation of sequence homologs lysozyme and α‐lactalbumin
P. Poole (1984)
10.1016/0011-2240(88)90054-5
Modes of stabilization of a protein by organic solutes during desiccation
J. F. Carpenter (1988)
10.1093/OXFORDJOURNALS.JBCHEM.A133468
Preferential hydration of bovine serum albumin in polyhydric alcohol-water mixtures.
K. Gekko (1981)
10.1016/0011-2240(88)90032-6
The mechanism of cryoprotection of proteins by solutes.
J. Carpenter (1988)
10.1016/0304-4157(88)90015-9
Interactions of sugars with membranes.
J. Crowe (1988)
10.1071/CH9681451
The control of phosphate contamination in an acid alumina process
P. Davey (1968)
10.1038/284372A0
Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences
C. M. Rubin (1980)
10.1139/V79-428
The correlation between O—H stretching frequencies and hydrogen bond distances in a crystalline sugar monohydrate
J. Umemura (1979)
10.1016/0003-9861(83)90201-1
Preferential interactions of proteins with solvent components in aqueous amino acid solutions.
T. Arakawa (1983)
10.1016/0003-9861(86)90755-1
Cryoprotection of phosphofructokinase with organic solutes: characterization of enhanced protection in the presence of divalent cations.
J. Carpenter (1986)
10.1021/BI00268A034
Preferential interactions of proteins with salts in concentrated solutions.
T. Arakawa (1982)
10.1016/0065-227X(83)90008-4
Water and proteins. II. The location and dynamics of water in protein systems and its relation to their stability and properties.
J. Edsall (1983)
10.1016/0011-2240(87)90049-6
Stabilization of phosphofructokinase during air-drying with sugars and sugar/transition metal mixtures.
J. Carpenter (1987)
10.1042/BJ2420001
Stabilization of dry phospholipid bilayers and proteins by sugars.
J. Crowe (1987)
10.1016/0005-2795(75)90122-1
Infrared spectroscopy of the water vapor sorption process of caseins.
M. Rueegg (1975)
10.1016/0141-8130(83)90047-8
Hydration-induced conformational and flexibility changes in lysozyme at low water content
P. Poole (1983)
10.1016/S0008-6215(00)82465-9
Infrared and raman spectroscopy of carbohydrates : Part IV. Identification of configuration- and conformation-sensitive modes for D-glucose by normal coordinate analysis
J. J. Cael (1974)
10.1038/284572A0
Correlation of IR spectroscopic, heat capacity, diamagnetic susceptibility and enzymatic measurements on lysozyme powder
G. Careri (1980)
10.1071/CH9681257
Spectra in the O-H stretching region of mono- and oligo-saccharides at low temperatures
A. Michell (1968)
10.1016/S0006-3495(85)83932-1
The stabilization of proteins by osmolytes.
T. Arakawa (1985)
10.1016/0003-9861(85)90628-9
Interaction of carbohydrates with dry dipalmitoylphosphatidylcholine.
L. Crowe (1985)
10.1002/BIP.1979.360180512
Lysozyme film hydration events: An IR and gravimetric study
G. Careri (1979)
The stabilization of proteins by sucrose.
J. Lee (1981)
10.1007/978-3-642-70613-4_39
Molecular Basis of the Biological Compatibility of Nature’s Osmolytes
P. S. Low (1985)
10.1016/S0008-6215(00)85035-1
Study of hydrogen bonds in cyrstalline monosaccharides the spectra of monosaccharides derivatives in the middle-and far-infrared regions
G. A. Kogan (1973)
10.1088/0022-3735/15/10/028
A direct difference infrared cell for studying the hydration of protein glasses.
P. Poole (1982)
10.1021/BI00268A033
Stabilization of protein structure by sugars.
T. Arakawa (1982)
10.1042/BJ2330025
Infra-red and Raman spectroscopic studies of enzyme structure and function.
C. Wharton (1986)



This paper is referenced by
10.1007/s13197-020-04705-x
Optimization of wall material for phage encapsulation via freeze-drying and antimicrobial efficacy of microencapsulated phage against Salmonella
K. Petsong (2020)
10.1016/0040-4039(90)80090-9
Accelerated esterification of aminoacids with lipoglycosylated α-chymotrypsin in polar solvents.
D. Cabaret (1990)
10.1111/J.1432-1033.1997.00024.X
Carbohydrate protection of enzyme structure and function against guanidinium chloride treatment depends on the nature of carbohydrate and enzyme.
M. Sola-Penna (1997)
10.1006/ABBI.1999.1175
Hydrogen bonding between sugar and protein is responsible for inhibition of dehydration-induced protein unfolding.
S. D. Allison (1999)
10.1063/1.1870872
Role of hydrogen bonds in the fast dynamics of binary glasses of trehalose and glycerol: a molecular dynamics simulation study.
T. Dirama (2005)
10.1002/CPHY.CP130220
Anhydrobiosis: Cellular Adaptation to Extreme Dehydration
J. Crowe (2011)
Development, characterisation and evaluation of sugar glass microneedles
C. Martin (2012)
10.1007/s00726-007-0506-3
Biotechnology applications of amino acids in protein purification and formulations
T. Arakawa (2007)
10.1016/J.VACCINE.2007.06.054
Rational design of an influenza subunit vaccine powder with sugar glass technology: preventing conformational changes of haemagglutinin during freezing and freeze-drying.
J-P. Amorij (2007)
10.1039/c5cc03274a
Room temperature freezing and orientational control of surface-immobilized peptides in air.
Y. Li (2015)
10.1586/14760584.2014.907528
Physicochemical and functional characterization of vaccine antigens and adjuvants
A. Dey (2014)
10.1016/J.VIBSPEC.2014.01.012
Studying the release of hGH from gamma-irradiated PLGA microparticles using ATR-FTIR imaging
H. Keleş (2014)
10.1016/J.JFOODENG.2009.08.012
Effect of protein on sorption characteristics of delactosed permeate
R. Bund (2010)
10.1016/j.ijpharm.2016.03.027
Development and potential application of an oral ColoPulse infliximab tablet with colon specific release: A feasibility study.
J. M. Maurer (2016)
10.1016/j.ab.2009.10.016
Recovery of tubulin functions after freeze-drying in the presence of trehalose.
E. Dráberová (2010)
10.1016/j.bbapap.2013.01.020
Unraveling protein stabilization mechanisms: vitrification and water replacement in a glass transition temperature controlled system.
N. Grasmeijer (2013)
10.1016/j.bbapap.2015.05.002
The intrinsically disordered protein LEA7 from Arabidopsis thaliana protects the isolated enzyme lactate dehydrogenase and enzymes in a soluble leaf proteome during freezing and drying.
A. V. Popova (2015)
OBTAINING DURABLE ENZYME POWDER VIA SPRAY DRYING
(2005)
Protein therapeutics in the solid state
Ukrit Angkawinitwong (2014)
Role of Lipids and Fatty Acids in Stress Tolerance in Cyanobacteria
S. Singh (2002)
10.1016/S1095-6433(01)00503-7
Lessons from nature: the role of sugars in anhydrobiosis.
L. Crowe (2002)
10.1016/S0008-6215(02)00284-7
Supercritical CO2 fluid extraction of crystal water from trehalose dihydrate. Efficient production of form II (Tα) phase
K. Akao (2002)
10.1023/A:1016043114998
Development of a Stable Freeze-dried Formulation of Recombinant Human Interleukin-1 Receptor Antagonist
B. Chang (2004)
10.1252/JCEJ.31.325
Influence of Protein on Phase Transition of Amorphous Sugar
K. Imamura (1998)
10.1016/S0006-3495(99)77208-5
Heme-solvent coupling: a Mössbauer study of myoglobin in sucrose.
H. Lichtenegger (1999)
10.1104/PP.120.1.153
Changed properties of the cytoplasmic matrix associated with desiccation tolerance of dried carrot somatic embryos. An In situ fourier transform infrared spectroscopic study
Wolkers (1999)
10.1016/S0378-5173(00)00423-3
Lyophilization and development of solid protein pharmaceuticals.
W. Wang (2000)
10.1016/S0264-410X(00)00240-1
Stabilisation of Salmonella vaccine vectors by the induction of trehalose biosynthesis.
H. Bullifent (2000)
10.1016/J.IJPHARM.2004.11.014
Protein aggregation and its inhibition in biopharmaceutics.
W. Wang (2005)
10.1016/j.ejpb.2007.12.003
The effects of cryoprotectants on the freeze-drying of ibuprofen-loaded solid lipid microparticles (SLM).
L. Zhang (2008)
10.1211/jpp.61.11.0016
The relation between moisture‐induced aggregation and structural changes in lyophilized insulin
Giselle M. Flores-Fernández (2009)
10.1002/9780470595886.CH22
Structural Analysis of Proteins in Dried Matrices
A. Hawe (2010)
See more
Semantic Scholar Logo Some data provided by SemanticScholar