Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

A Procedure For The Rapid, Large-scall Purification Of Escherichia Coli DNA-dependent RNA Polymerase Involving Polymin P Precipitation And DNA-cellulose Chromatography.

R. Burgess, J. Jendrisak
Published 1975 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
An improved method is described for the purification of the DNA-dependent RNA polymerase [ribonucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6] from Escherichia coli. The method involves lysozyme-sodium deoxycholate lysis, low-speed centrifugation, precipitation with Polymin P, elution from the Polymin P precipitate, ammonium sulfate precipitation, and chromatography on DNA-cellulose and Bio-Gel A 5m. RNA polymerase is purified to electrophoretic homogeneity in 2 days with a recovery of 45%, resulting in a yield of 250 mg of holoenzyme from 500 g of cells.
This paper references
10.1021/JA01503A008
Effect of Light Scattering on Ultraviolet Difference Spectra1
S. Leach (1960)
10.1042/BJ1330201
A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
P. Humphries (1973)
10.1016/0926-6550(63)90342-6
Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174.
G. Guthrie (1963)
10.1016/S0076-6879(71)21013-2
[11] DNA-cellulose chromatography
B. Alberts (1971)
10.1038/227680A0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
10.1128/JB.97.3.1437-1443.1969
Characterization of polynucleotide phosphorylase mutants of Escherichia coli.
A. M. Reiner (1969)
10.1016/S0006-291X(72)80040-8
Chromatography of rNA polymerase from escherichia coli on single stranded DNA-agarose columns.
C. Nüsslein (1972)
10.1016/0006-291X(73)90899-1
Separation of RNA polymerase holoenzyme from core enzyme on DNA-cellulose column.
R. Mukai (1973)
10.1038/NEWBIO234051A0
Effects of protease inhibitors on protein breakdown and enzyme induction in starving Escherichia coli.
A. Goldberg (1971)
10.1016/s0021-9258(18)63520-3
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
R. Burgess (1969)
10.1016/S0076-6879(71)21044-2
[42] Purification of the RNA polymerase sigma factor☆
R. Burgess (1971)
10.1515/BCHM2.1970.351.1.221
A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.
W. Zillig (1970)
The reversible denaturation of deoxyribonucleic acid-dependent ribonucleic acid polymerase of Escherichia coli.
L. Yarbrough (1974)
10.1073/PNAS.55.6.1616
Some physical properties of RNA polymerase.
John P. Richardson (1966)
10.1016/0006-291X(69)90292-7
DNA-dependent RNA polymerase from E. coli: Studies on the role of σ in chain initiation
J. Dunn (1969)
10.1016/s0021-9258(18)91873-9
Properties of chromatographically purified bovine pancreatic deoxyribonuclease.
P. Price (1969)
10.1042/BJ0960671
The sedimentation behaviour of ribonuclease-active and -inactive ribosomes from bacteria.
K. A. Cammack (1965)
10.1016/s0021-9258(19)52451-6
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)



This paper is referenced by
10.1016/S0076-6879(03)70003-2
Determination of Escherichia coli RNA polymerase structure by single particle cryoelectron microscopy.
P. Ray (2003)
10.1111/j.1365-2958.2010.07073.x
Identification and characterization of E. coli CRISPR‐cas promoters and their silencing by H‐NS
Ümit Pul (2010)
10.1016/0014-5793(82)80446-8
Purification involving polymin P fractionation of Escherichia coli DNA polymerase III which shows a high sedimentation constant
Y. Kobayashi (1982)
10.1128/JB.01753-14
Transcription start site sequence and spacing between the -10 region and the start site affect reiterative transcription-mediated regulation of gene expression in Escherichia coli.
X. Han (2014)
10.1016/0014-5793(77)80162-2
The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine
A. H. Bingham (1977)
10.1016/0022-2836(78)90054-2
Effect of the SP01 DNA-binding protein, TF1, on promoter competition.
E. Geiduschek (1978)
10.1016/0378-1119(87)90002-3
RNA polymerase makes important contacts upstream from base pair -49 at the Escherichia coli galactose operon P1 promoter.
S. Busby (1987)
10.1111/J.1574-6968.1986.TB01528.X
DNA‐dependent RNA polymerase from Spirochaeta aurantia
B. Allan (1986)
10.18388/ABP.2009_2504
Evaluation of mixed-salt effects on thermodynamic and kinetic parameters of RNA polymerase-promoter DNA complexes in terms of equivalent salt concentrations. General applicability to DNA complexes.
Tomasz Łoziński (2009)
10.1016/0022-2836(84)90434-0
Control of RNA polymerase binding to chromatin by variations in linker histone composition.
R. Hannon (1984)
10.1111/j.1365-2958.1995.tb02441.x
Characterization of heat‐shock response of the marine bacterium Vibrio harveyi
G. Klein (1995)
10.1016/0022-0248(91)90891-8
Preparation of crystals of T7 RNA polymerase suitable for high-resolution X-ray structure analysis
R. Sousa (1991)
10.1016/0162-0134(86)80079-4
Zinc metalloproteins involved in replication and transcription.
D. Giedroc (1986)
10.1016/0022-2836(92)90388-Z
DNA supercoiling response of the σ54-dependent Klebsiella pneumoniae nifL promoter in vitro
S. Whitehall (1992)
10.1016/J.JMB.2005.03.042
A quantitative description of the binding states and in vitro function of antitermination protein N of bacteriophage lambda.
C. R. Conant (2005)
Reiterative Transcription Occurs in Part through UTP-Sensitive coli Escherichia Expression in carAB Regulation of
Xiaosi Han (1998)
10.1021/BI00549A021
Functional heterogeneity of Escherichia coli ribonucleic acid polymerase holoenzyme.
A. Travers (1980)
10.1016/S0006-291X(82)80009-0
Effects of sigma subunit and DNA template on the accessibility of rifamycin bound to RNA polymerase.
L. S. Rice (1982)
10.1021/BI00075A003
Promoter recognition by Escherichia coli RNA polymerase. Effects of single base pair deletions and insertions in the spacer DNA separating the -10 and -35 regions are dependent on spacer DNA sequence.
S. Warne (1993)
10.1021/BI00634A027
Effect of proteolysis of transcriptional fidelity of reconstituted chromatin.
R. A. Gadski (1977)
10.1002/j.1460-2075.1995.tb00190.x
Both ambient temperature and the DnaK chaperone machine modulate the heat shock response in Escherichia coli by regulating the switch between sigma 70 and sigma 32 factors assembled with RNA polymerase.
A. Blaszczak (1995)
10.1021/BI00265A051
Chloroacetaldehyde-treated ribo- and deoxyribopolynucleotides. 2. Errors in transcription by different polymerases resulting from ethenocytosine and its hydrated intermediate.
J. Kuśmierek (1982)
10.1128/JB.00902-09
Mechanism of transcriptional activation by Pseudomonas aeruginosa ExsA.
Christopher A Vakulskas (2009)
10.1038/302074A0
Functional interrelationship between two tandem E. coli ribosomal RNA promoters
G. Glaser (1983)
10.1016/0005-2787(81)90194-5
An RNA-dependent ATPase from Chlamydomonas reinhardII.
A. Chu (1981)
10.1016/j.ymeth.2008.10.018
Analysis of RNA polymerase-promoter complex formation.
W. Ross (2009)
10.1016/j.bbagrm.2012.11.008
Characterization of the transcriptional stimulatory properties of the Pseudomonas putida RapA protein.
Ewa Stec-Dziedzic (2013)
10.1016/S0092-8674(00)80873-9
6S RNA Regulates E. coli RNA Polymerase Activity
K. Wassarman (2000)
10.1074/JBC.275.5.3661
Identification of RNA Polymerase β′ Subunit Segment Contacting the Melted Region of the lacUV5 Promoter*
K. Brodolin (2000)
10.1074/jbc.273.24.15157
Disruption of Escherichia coli HepA, an RNA Polymerase-associated Protein, Causes UV Sensitivity*
O. Muzzin (1998)
10.1073/PNAS.94.25.13548
Escherichia coli RNA polymerase terminates transcription efficiently at rho-independent terminators on single-stranded DNA templates.
S. M. Uptain (1997)
10.1101/GAD.7.1.161
Elongation factor NusG interacts with termination factor rho to regulate termination and antitermination of transcription.
J. Li (1993)
See more
Semantic Scholar Logo Some data provided by SemanticScholar