← Back to Search
Polysaccharide-oligoamine Based Conjugates For Gene Delivery.
T. Azzam, H. Eliyahu, L. Shapira, M. Linial, Y. Barenholz, A. Domb
Published 2002 · Chemistry, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
This work describes a versatile and universal polycation system based on oligoamines grafted on natural polysaccharides that is capable of complexing various plasmids and administering them into various cells in high yield to produce a desired protein. These polycations are expected to better meet the requirements for effective complexation and delivery of plasmid or an antisense and to biodegrade into nontoxic components at a controlled rate. The developed biodegradable polycations are based on spermine, a natural tetramine, conjugated to dextran or arabinogalactan. These polycations were prepared by reductive amination of oxidized polysaccharides with the desired oligoamines. The Schiff base conjugates thus obtained were reduced to the stable amine conjugates by sodium borohydride. Over 300 different polycations were prepared starting from various polysaccharides and oligoamines, mainly oligoamines of two to four amino groups. Although most of these conjugates formed stable complexes with various plasmids as determined by turbidity experiments, only a few polycations were found to be active in transfecting cells. This work indicates that the structure of the polycation plays a significant role in the transfection activity of polycations.
This paper references
Dextran prodrugs; Villadsen and Christesen
C Larsen (1990)
Human gene therapy.
W. Anderson (1992)
Gene therapy of cancer.
C. Mullen (1996)
Synthesis and evaluation of poly(ethylene glycol)-polylysine copolymers as carriers for gene delivery Optimized galenics improve in-vitro gene transfer with cationic molecules up to a 1000-fold
S Vanderkerken (1996)
Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold.
O. Boussif (1996)
Nonviral gene therapy: the promise of genes as pharmaceutical products.
F. Ledley (1995)
Pharmaceutical and biological properties of poly(amino acid)/DNA polyplexes.
P. Lucas (1999)
DNA-polycation nanospheres as non-viral gene delivery vehicles.
K. Leong (1998)
Gene therapy for cancer: what have we done and where are we going?
J. Roth (1997)
Structure properties of dextran. 2. Dilute solution
Catalina E. Ioan (2000)
Effect of in situ retroviral interleukin-4 transfer on established intracranial tumors.
M. Saleh (1999)
Polyelectrolyte vectors for gene delivery: influence of cationic polymer on biophysical properties of complexes formed with DNA.
M. A. Wolfert (1999)
Liposome-mediated in vivo gene transfer of antisense K-ras construct inhibits pancreatic tumor dissemination in the murine peritoneal cavity.
K. Aoki (1995)
Cationic Polymer Based Gene
S. C. De Smedt (2000)
The TMC Worldwide Gene Therapy Enrollment Report (June 1996).
T. Marcel (1996)
Transfer of Genes to Humans: Early Lessons and Obstacles to Success
R. Crystal (1995)
Structures properties of dextran
C. E. Ioan (2000)
Nonviral vectors for gene therapy
L. Huang (1999)
Synthesis of some poly(4‐alkyl‐4‐azaheptamethylene‐D‐glucaramides)
A. Carter (2000)
A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: polyethylenimine.
B. Abdallah (1996)
Determination of Degree of Substitution of Formyl Groups in Polyaldehyde Dextran by the Hydroxylamine Hydrochloride Method
H. Zhao (2004)
Arabinogalactan for hepatic drug delivery.
E. Groman (1994)
In Polymers in Gene Therapy: Frontiers in Biological Polymer Application
A J Domb (1999)
The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes
M. Tang (1997)
The TMC Worldwide Gene Therapy Enrollment Report, end 1996.
T. Marcel (1996)
Cationic Polymer Based Gene Delivery Systems
S. D. De Smedt (2004)
Effect of Cation Content of Polycation-type Gene Carriers on in vitro Gene Transfer
T. Yamaoka (1998)
An improved 2,4,6-trinitrobenzenesulfonic acid method for the determination of amines.
S. Snyder (1975)
Polymers in Gene Therapy: Frontiers in Biological Polymer Application
A. J. Domb (1999)
Eradication of established intracranial rat gliomas by transforming growth factor beta antisense gene therapy.
H. Fakhrai (1996)
Gene therapy of maliganant brain tumors: A rat glioma line bearing the herpes simplex virus type 1‐thymidine kinase gene and wild type retrovirus kills other tumor cells
Y. Takamiya (1992)
Polymers in gene therapy: antisense delivery systems
Z. Israel (1998)
Regulation of in vitro gene expression using antisense oligonucleotides or antisense expression plasmids transfected using starburst PAMAM dendrimers.
A. Bielinska (1996)
Targeting gene therapy vectors to CNS malignancies.
M. Spear (1998)
Fluorescent neoglycoproteins: antibody-aminodextran-phycobiliprotein conjugates.
O. Siiman (1999)
binogalactan for hepatic drug delivery
E. V. Groman (1994)
A powerful nonviral vector for in-vivo gene transfer into the adult mammalian brain: Polyethyleneimine. Hum. Gene Ther
B Abdllah (1996)
An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery.
I. Koltover (1998)
Synthesis and Evaluation of Poly(Ethylene Glycol)-Polylysine Block Copolymers as Carriers for Gene Delivery
S. Vanderkerken (2000)
This paper is referenced by
Polycation-based gene therapy: current knowledge and new perspectives.
Marcio J Tiera (2011)
Struktur-Wirkungs-Beziehung peptidischer Guanidiniocarbonylpyrrol-Liganden zur nicht-viralen Gentransfektion – ein Balanceakt über einen Hindernisparcours
Sandra Junghänel (2017)
Promoted adipogenesis of rat mesenchymal stem cells by transfection of small interfering RNA complexed with a cationized dextran.
K. Nagane (2010)
POLYMERIC CORE‐SHELL NANOPARTICLES FOR THERAPEUTICS
Y. Yang (2006)
Polysaccharide gene transfection agents.
Wahid Khan (2012)
Poly(methyl methacrylate)-graft-oligoamines as low cytotoxic and efficient nonviral gene vectors.
Yong-Qiang Wang (2010)
Polyhydroxyethylaspartamide-spermine copolymers: efficient vectors for gene delivery.
G. Cavallaro (2008)
6-amino-6-deoxychitosan. preparation and application as plasmid vector in COS-1 cells
T. Satoh (2004)
A low-toxic and efficient gene vector: carboxymethyl dextran-graft-polyethylenimine.
Yun-xia Sun (2008)
Relationships between chemical composition, physical properties and transfection efficiency of polysaccharide-spermine conjugates.
H. Eliyahu (2006)
Dextran-graft-linear poly(ethylene imine)s for gene delivery: importance of the linking strategy.
Sofia Ochrimenko (2014)
Giant amino acids designed on the polysaccharide scaffold and their protein-like structural interconversion.
Shun-Ichi Tamaru (2014)
Structural transformations of arabinogalactan from the Siberian larch during mechanochemical processing and the biological properties of the products
E. N. Medvedeva (2010)
Modified pectin-based carrier for gene delivery: cellular barriers in gene delivery course.
Tali Katav (2008)
Ultrasound enhances in vivo tumor expression of plasmid DNA by PEG-introduced cationized dextran.
H. Hosseinkhani (2005)
Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan.
M. Mizu (2004)
An eco-friendly technology for polysaccharide production from logging and sawing waste
V. A. Babkin (2012)
Efficient gene delivery to mesenchymal stem cells by an ethylenediamine-modified polysaccharide from mulberry leaves.
W. Deng (2012)
Effect of lipopolysaccharide addition on the gene transfection of spermine-introduced pullulan-plasmid DNA complexes for human mesenchymal stem cells
Masumi Ueda (2019)
Glucan Particles for Macrophage Targeted Delivery of Nanoparticles
E. Soto (2012)
Recent advances in the development of gene delivery systems
Yk Sung (2019)
Surface Derivatization of Glucan Particles for Drug Delivery
Melissa K. Castle (2011)
Polysaccharide-Based Conjugates for Biomedical Applications This paper was originally submitted for the “ Biofunctional Biomaterials : The Third Generation of Medical Devices
Arijit Basu (2015)
Engineering of magnetic DNA nanoparticles for tumor-targeted therapy
H. Hosseinkhani (2013)
Degradable Piezoelectric Biomaterials for Wearable and Implantable Bioelectronics.
J. Li (2020)
Acid-degradable cationic dextran particles for the delivery of siRNA therapeutics.
J. Cohen (2011)
Dextran–spermine polycation: an efficient nonviral vector for in vitro and in vivo gene transfection
H. Hosseinkhani (2004)
Blood hot in Boston.
A. C. Hunter (2002)
Mechanical Composites of Rimantadine and Larch Arabinogalactan
V. Babkin (2014)
Preparation of Cationized Polysaccharides as Gene Transfection Carrier for Bone Marrow-Derived Mesenchymal Stem Cells
Jun-ichiro Jo (2010)
Decreased solute adsorption onto cracked surfaces of mechanically injured articular cartilage: towards the design of cartilage-specific functional contrast agents.
M. Moeini (2014)
A review on versatile applications of blends and composites of pullulan with natural and synthetic polymers.
S. Tabasum (2018)See more