Online citations, reference lists, and bibliographies.
← Back to Search

Layer By Layer Buildup Of Polysaccharide Films: Physical Chemistry And Cellular Adhesion Aspects.

L. Richert, P. Lavalle, E. Payan, X. Z. Shu, G. Prestwich, J. Stoltz, P. Schaaf, J. Voegel, C. Picart
Published 2004 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
The formation ofpolysaccharide films based on the alternate deposition of chitosan (CHI) and hyaluronan (HA) was investigated by several techniques. The multilayer buildup takes place in two stages: during the first stage, the surface is covered by isolated islets that grow and coalesce as the construction goes on. After several deposition steps, a continuous film is formed and the second stage of the buildup process takes place. The whole process is characterized by an exponential increase of the mass and thickness of the film with the number of deposition steps. This exponential growth mechanism is related to the ability of the polycation to diffuse "in" and "out" of the whole film at each deposition step. Using confocal laser microscopy and fluorescently labeled CHI, we show that such a diffusion behavior, already observed with poly(L-lysine) as a polycation, is also found with CHI, a polycation presenting a large persistence length. We also analyze the effect of the molecular weight (MW) of the diffusing polyelectrolyte (CHI) on the buildup process and observe a faster growth for low MW chitosan. The influence of the salt concentration during buildup is also investigated. Whereas the CHI/HA films grow rapidly at high salt concentration (0.15 M NaCl) with the formation of a uniform film after only a few deposition steps, it is very difficult to build the film at 10(-4) M NaCl. In this latter case, the deposited mass increases linearly with the number of deposition steps and the first deposition stage, where the surface is covered by islets, lasts at least up to 50 bilayer deposition steps. However, even at these low salt concentrations and in the islet configuration, CHI chains seem to diffuse in and out of the CHI/HA complexes. The linear mass increase of the film with the number of deposition steps despite the CHI diffusion is explained by a partial redissolution of the CHI/HA complexes forming the film during different steps of the buildup process. Finally, the uniform films built at high salt concentrations were also found to be chondrocyte resistant and, more interestingly, bacterial resistant. Therefore, the (CHI/HA) films may be used as an antimicrobial coating.



This paper is referenced by
10.1002/ADFM.200400588
Controlled Degradability of Polysaccharide Multilayer Films In Vitro and In Vivo
C. Picart (2005)
10.32657/10356/43517
Layer-by-layer self-assembled weak polyelectrolyte-based novel multilayered film : fabrication, unique properties and applications.
Wei-yong Yuan (2010)
10.1016/j.colsurfb.2010.01.006
Layer-by-layer assembly of polysaccharide-based nanostructured surfaces containing polyelectrolyte complex nanoparticles.
Soheil Boddohi (2010)
NANODIAMOND PARTICLES FOR BIOMACROMOLECULE IMMOBILIZATION AND DYE CONTAMINANT ADSORPTION
Hai-dong Wang (2011)
10.3390/POLYM6051502
Polyelectrolyte Multilayers: Towards Single Cell Studies
D. Volodkin (2014)
10.1016/J.FOODHYD.2019.04.037
From beta-cyclodextrin polyelectrolyte to layer-by-layer self-assembly microcapsules: From inhibition of bacterial growth to bactericidal effect
S. Belbekhouche (2019)
10.1002/mabi.201000298
Development of a new polypropylene-based suture: plasma grafting, surface treatment, characterization, and biocompatibility studies.
Shalini Saxena (2011)
10.1021/am404268z
Fabrication of ultrathin membrane via layer-by-layer self-assembly driven by hydrophobic interaction towards high separation performance.
Jing Zhao (2013)
10.1021/bm200681m
Nanoparticle-based biocompatible and targeted drug delivery: characterization and in vitro studies.
Xiao Yu (2011)
The Effect of Various Concentrations of Adlay Seed Extract Polyelectrolyte Multilayers Film Coated on Titanium Surface to Hydrophilicity and Osteoblast Cell Viability and In Vitro Calcification
(2018)
10.1016/J.JCIS.2007.01.086
The salivary mucin MUC5B and lactoperoxidase can be used for layer-by-layer film formation.
L. Lindh (2007)
10.1002/MASY.201050812
Biogenic Polyelectrolyte Multilayers on Poly(L-lactide) Films for Control of Osteoblast Adhesion
Zhen-Mei Liu (2010)
10.1002/adma.201001747
Engineering the extracellular environment: Strategies for building 2D and 3D cellular structures.
Orane Guillame-Gentil (2010)
10.1002/ADMI.201400237
“Smart” Surface Capsules for Delivery Devices
E. Skorb (2014)
10.1021/bm501751v
Targeted release of tobramycin from a pH-responsive grafted bilayer challenged with S. aureus.
Hyun-Su Lee (2015)
10.1116/1.4943046
What is really driving cell-surface interactions? Layer-by-layer assembled films may help to answer questions concerning cell attachment and response to biomaterials.
Y. Sergeeva (2016)
10.1016/j.colsurfb.2016.02.028
Hyaluronan/chitosan nanofilms assembled layer-by-layer and their antibacterial effect: A study using Staphylococcus aureus and Pseudomonas aeruginosa.
J. Hernández-Montelongo (2016)
10.1016/j.carbpol.2019.115162
Biodegradable free-standing films from lauroyl derivatives of hyaluronan.
Josef Chmelař (2019)
10.1115/SBC2012-80206
Polyelectrolyte Multilayer Nanoshells With Hydrophobic Nanodomains for Delivery of Paclitaxel
T. Boudou (2012)
10.1021/CM4019925
Cyclodextrin/Paclitaxel Complex in Biodegradable Capsules for Breast Cancer Treatment
J. Jing (2013)
10.1016/j.biomaterials.2009.05.026
Effect of genipin cross-linking on the cellular adhesion properties of layer-by-layer assembled polyelectrolyte films.
Anna L Hillberg (2009)
10.1002/JBM.A.31102
Layer-by-layer assembly of biomacromolecules on poly(ethylene terephthalate) films and fiber fabrics to promote endothelial cell growth.
Y. Liu (2007)
10.1016/J.JCIS.2007.07.071
Alternating bioactivity of multilayer thin films assembled from charged derivatives of chitosan.
Somruethai Channasanon (2007)
Interactions of Mucins with Biopolymers and Drug Delivery Particles
O. Svensson (2008)
Modulatingthereleasekinetics throughthecontrolofthe permeabilityofthelayer-by-layer assembly:areview
Sania Mansouri (2009)
10.1002/MACP.200900053
Microstructure and Molecular Interaction in Glycerol Plasticized Chitosan/Poly(vinyl alcohol) Blending Films
S. Liang (2009)
10.1002/adma.201001215
Antifouling coatings: recent developments in the design of surfaces that prevent fouling by proteins, bacteria, and marine organisms.
I. Banerjee (2011)
10.1002/9781118229347.CH10
Quartz Crystal Microbalance with Dissipation
Boce Zhang (2012)
10.1081/e-escs3-120045329
Drug Delivery: Polyelectrolyte Multilayers
Dmitry Volodkin (2015)
10.2147/MDER.S13962
Ionically cross-linked hyaluronic acid: wetting, lubrication, and viscoelasticity of a modified adhesion barrier gel
Katherine Vorvolakos (2011)
10.1088/1748-6041/2/1/S07
Elasticity, biodegradability and cell adhesive properties of chitosan/hyaluronan multilayer films.
Aurore Schneider (2007)
10.1016/J.COCIS.2010.07.006
Swelling of electrochemically active polyelectrolyte multilayers
Raphael Zahn (2010)
See more
Semantic Scholar Logo Some data provided by SemanticScholar