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Assessment Of Fluorescence Resonance Energy Transfer For Two-color DNA Microarray Platforms.

J. Zhu, Y. Lu, Cheng Deng, G. Huang, Shengyi Chen, Shukuan Xu, Yi Lv, K. Mitchelson, J. Cheng
Published 2010 · Chemistry, Medicine

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Two-color DNA microarray platforms are widely used for determining differential amounts of target sequences in parallel between sample pairs. However, the fluorescence (or Forster) resonance energy transfer (FRET) between two fluorophores can potentially result in the distortions of the measured fluorescence signals. Here we assessed the influence of FRET on the two-color DNA microarray platform and developed a reliable and convenient method for the correction of FRET distortion. Compared to current methods of normalization based on the statistical analysis and the hypothesis that only a small part of target sequences are differentially presented between sample pairs, our FRET correction method can recover the undistorted signals by the compensation of fluorescence emission, without considering the number of target sequences differentially presented. The correction method was validated with samples at different target ratios and with microarrays spotted in different probe concentrations. We also applied the FRET correction method to gene expression profiling arrays, and the results show that FRET was present when the content of target sequence was beyond a threshold amount and that the process incorporating our FRET correction method can improve the reliability of the gene expression profiling microarray platform in comparison with the current process without FRET correction.



This paper is referenced by
10.1039/c2an15902c
General and reliable quantitative measurement of fluorescence resonance energy transfer using three fluorescence channels.
Fengbo Xie (2012)
10.1021/MZ500378C
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Chen Xu (2014)
10.1021/acs.analchem.6b04596
Label-Free Method Using a Weighted-Phase Algorithm To Quantitate Nanoscale Interactions between Molecules on DNA Microarrays.
Q. Li (2017)
10.1016/j.talanta.2011.11.041
FRET detection of DNA sequence via electrostatic interaction of polycationic phenyleneethynylene dendrimer with DNA/PNA hybrid.
Paitoon Rashatasakhon (2012)
10.1117/12.2068706
Screening mTOR siRNA based on bioinformatics and detecting the transcription by the gold nanoparticle beacon
Caiping Tian (2014)
10.1002/cpch.87
Three‐Color Imaging Enables Simultaneous Screening of Multiple RNA Targets on Small Molecule Microarrays
Deondre Jordan (2020)
10.1039/C6RA28270A
Identification of DNA single-base mismatches by resistivity of poly(N-isopropylacrylamide)-block-ssDNA copolymer brush films at dual temperatures
Y. Liu (2017)
10.1016/j.bios.2014.10.029
Labeling-free fluorescent detection of DNA hybridization through FRET from pyrene excimer to DNA intercalator SYBR green I.
R. Zhou (2015)
10.1021/ac300551e
Microchip device with 64-site electrode array for multiplexed immunoassay of cell surface antigens based on electrochemiluminescence resonance energy transfer.
Meisheng Wu (2012)
10.1002/9783527656028.CH07
In Vitro FRET Sensing, Diagnostics, and Personalized Medicine
S. Spindel (2013)
DNA single-base mismatches by resistivity of poly ( N-isopropylacrylamide )-block-ssDNA copolymer brush fi lms at dual temperatures †
Y. K. Liu (2017)
10.1016/j.bios.2013.10.032
Label-free DNA detection using two-dimensional periodic relief grating as a visualized platform for diagnosis of breast cancer recurrence after surgery.
J. Chen (2014)
10.1016/j.bios.2012.06.062
Visual detection of STAT5B gene expression in living cell using the hairpin DNA modified gold nanoparticle beacon.
Jianpeng Xue (2013)
10.1016/j.talanta.2017.12.066
An interferometric imaging biosensor using weighted spectrum analysis to confirm DNA monolayer films with attogram sensitivity.
Rongxin Fu (2018)
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