Online citations, reference lists, and bibliographies.

Cytochrome C Mutants For Superoxide Biosensors.

Franziska Wegerich, Paola Turano, Marco Allegrozzi, Helmut Moehwald, Fred Lisdat
Published 2009 · Chemistry, Medicine
Cite This
Download PDF
Analyze on Scholarcy
Share
The effect of introducing positive charges (lysines) in human cytochrome c (cyt c) on the redox properties and reaction rates of cyt c with superoxide radicals was studied. The mutated forms of this electron-transfer protein are used as sensorial recognition elements for the amperometric detection of the reactive oxygen radical. The proteins were prepared by site-directed mutagenesis focusing on amino acids near the heme edge. The 11 mutants of human cyt c expressed in the course of this research have been characterized by UV-vis spectroscopy, circular dichroism, and NMR spectroscopy to verify overall structure integrity as well as axial coordination of the heme iron. The mutants are investigated voltammetrically using promoter-modified gold electrodes with respect to redox activity and formal redox potential. The rate constants for the reaction with superoxide have been determined spectrophotometrically. Four mutants show a higher reaction rate with the radical as compared to the wild type. These mutants are used for the construction of superoxide sensors based on thiol-modified gold electrodes and covalently fixed proteins. We found that the E66K mutant-based electrode has a clearly higher sensitivity in comparison with the wild-type-based sensor while retaining the high selectivity and showing a good storage stability.



This paper is referenced by
10.1007/s00775-012-0946-4
Versatility of non-native forms of human cytochrome c: pH and micellar concentration dependence
Matthieu Simon (2012)
10.1039/c4nr05301j
Study of cytochrome c-DNA interaction--evaluation of binding sites on the redox protein.
Christoph Wettstein (2014)
10.1039/C7NJ02741A
The cytochrome c–cyclo[6]aramide complex as a supramolecular catalyst in methanol
Wang Pan (2018)
10.1016/j.jinorgbio.2014.02.015
Nitrate as a probe of cytochrome c surface: crystallographic identification of crucial "hot spots" for protein-protein recognition.
Matteo De March (2014)
10.1016/J.ACA.2019.04.035
Nanotoxicity assessment: A challenging application for cutting edge electroanalytical tools.
Francesca Bettazzi (2019)
10.1089/ars.2010.3356
High-mobility group box 1, oxidative stress, and disease.
Daolin Tang (2011)
10.1007/s00775-012-0958-0
Elucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin
Andrej Musatov (2012)
10.1016/S1872-2040(13)60701-X
Progress on Electrochemical Determination of Superoxide Anion
Zhen Wang (2014)
10.1021/cr400518y
How biology handles nitrite.
Luisa B Maia (2014)
10.1039/c1an15380c
Superoxide radical biosensor based on a nano-composite containing cytochrome c.
Parvaneh Rahimi (2011)
10.1007/s00775-012-0915-y
Site-specific covalent attachment of heme proteins on self-assembled monolayers
Sohini Mukherjee (2012)
10.1021/ac300255n
Simultaneous determination of reactive oxygen and nitrogen species in mitochondrial compartments of apoptotic HepG2 cells and PC12 cells based on microchip electrophoresis-laser-induced fluorescence.
Zhenzhen Chen (2012)
10.1039/c0cc00850h
Bidirectional immobilization of affinity-tagged cytochrome c on electrode surfaces.
Florian Schroeper (2010)
10.3390/ijms21062134
Distal Unfolding of Ferricytochrome c Induced by the F82K Mutation
Daniela Lalli (2020)
10.1021/ac301086m
Stability and sensitivity enhanced electrochemical in vivo superoxide microbiosensor based on covalently co-immobilized lipid and cytochrome c.
Md Aminur Rahman (2012)
10.1039/c0cp00793e
Insights into the formation and operation of polyaniline sulfonate/cytochrome c multilayer electrodes: contributions of polyelectrolytes' properties.
David Sarauli (2010)
10.1021/BK-2015-1200.CH013
Electrochemical Biosensors for Real-Time Monitoring of Reactive Oxygen and Nitrogen Species
Xiaobo Liu (2015)
10.1021/ja5112628
Single gold@silver nanoprobes for real-time tracing the entire autophagy process at single-cell level.
Zixuan Chen (2015)
10.1007/s00216-013-6789-1
Quantum dots on electrodes—new tools for bioelectroanalysis
Fred Lisdat (2013)
10.1007/s00775-013-1026-0
Cytochrome c and superoxide: a reply
Franziska Wegerich (2013)
10.1016/j.ab.2011.08.048
Pyrogallol red oxidation induced by superoxide radicals: application to evaluate redox cycling of nitro compounds.
Mario A Faúndez (2011)
10.1016/B978-0-444-53794-2.00011-2
Detection of Biomarkers for Different Diseases on Biosensor Surfaces Part II
Ajit Sadana (2015)
10.1002/ELAN.201200481
Cytochrome c‐Based Amperometric Sensors for Superoxide Detection: Where Their Signal Comes From?
Szilveszter Gáspár (2013)
10.1016/J.BIOELECHEM.2019.04.021
Fabrication of a κ-carrageenan-based electroactive cytochrome c multilayer thin film by an electrostatic layer-by-layer assembly.
Zülfikar Temoçin (2019)
10.1021/acsomega.6b00213
Insights into Interprotein Electron Transfer of Human Cytochrome c Variants Arranged in Multilayer Architectures by Means of an Artificial Silica Nanoparticle Matrix
Sven Christian Feifel (2016)
10.1002/ELAN.201300526
Nanohybrid Materials Consisting of Poly[(3‐aminobenzoic acid)‐co‐(3‐aminobenzenesulfonic acid)‐co‐aniline] and Multiwalled Carbon Nanotubes for Immobilization of Redox Active Cytochrome c
Johannes Tanne (2014)
10.1016/j.bbagen.2018.03.010
Polyethylene glycol promotes autoxidation of cytochrome c.
Wataru Sato (2018)
10.1073/pnas.1618008114
Structural basis of mitochondrial dysfunction in response to cytochrome c phosphorylation at tyrosine 48
Blas Moreno-Beltrán (2017)
10.1007/s11051-012-1321-7
Controlled adsorption of cytochrome c to nanostructured gold surfaces
Inês Gomes (2012)
10.1142/S1088424612501404
Electron self-exchange of cytochrome c measured via13C detected protonless NMR
Stefano Cacciatore (2013)
Micron-scale flexible electronics for detection of reactive oxygen species for in vivo applications
Rebekah C. Wilson (2010)
10.1021/acs.analchem.5b03664
Consecutive Gated Injection-Based Microchip Electrophoresis for Simultaneous Quantitation of Superoxide Anion and Nitric Oxide in Single PC-12 Cells.
Lu Li (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar