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Ratiometric Visualization Of NO/H2S Cross-Talk In Living Cells And Tissues Using A Nitroxyl-Responsive Two-Photon Fluorescence Probe.

Y. Zhou, Xiufang Zhang, Sheng Yang, Y. Li, Z. Qing, J. Zheng, J. Li, R. Yang
Published 2017 · Chemistry, Medicine

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It is of scientific significance to explore the intricate relationship between two crucial gasotransmitters nitric oxide (NO) and hydrogen sulfide (H2S) because they exert similar and interdependent biological actions within the living organisms. Nevertheless, visualization of the NO/H2S crosstalk using effective molecular imaging tools remains challenging. To address this issue, and given that nitroxyl (HNO) has been implicated as the interdependent production of NO and H2S via a network of cascading chemical reactions, we herein design a ratiometric two-photon fluorescent probe for HNO, termed TP-Rho-HNO, which consists of benzo[h]chromene-rhodol scaffold as two-photon energy transfer cassette with phosphine moiety as specific HNO recognition unit. The newly proposed probe has been successfully applied in ratiometric two-photon bioimaging of endogenous HNO derived from NO and H2S interaction in the human umbilical vein cells (HUVECs) and as well as in rat brain tissues. Intriguingly, the imaging results consistently demonstrate that the mutually dependent upgeneration of H2S and NO are present in living biosystems, indicating that this molecular probe would provide a powerful approach to elucidate the chemical foundation for the anfractuous cross-talk between the NO and H2S signaling pathways in biology.
This paper references
10.1021/ac503806w
Toward a selective, sensitive, fast-responsive, and biocompatible two-photon probe for hydrogen sulfide in live cells.
S. Singha (2015)
10.1021/jo201504h
Two-photon Lysotrackers for in vivo imaging.
J. H. Son (2011)
10.1021/ja909148v
Direct detection of nitroxyl in aqueous solution using a tripodal copper(II) BODIPY complex.
Joel Rosenthal (2010)
10.1021/ol202706k
Development of FRET-based ratiometric fluorescent Cu2+ chemodosimeters and the applications for living cell imaging.
L. Yuan (2012)
10.1016/j.vph.2015.03.004
Gender specific generation of nitroxyl (HNO) from rat endothelium.
K. Hamilton (2015)
10.1016/j.pharmthera.2009.05.005
Actions and interactions of nitric oxide, carbon monoxide and hydrogen sulphide in the cardiovascular system and in inflammation--a tale of three gases!
L. Li (2009)
10.1039/C3SC50369K
A near-infrared ratiometric fluorescent probe for rapid and highly sensitive imaging of endogenous hydrogen sulfide in living cells
X. Wang (2013)
10.1093/emboj/20.21.6008
The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener
W. Zhao (2001)
10.1021/OL702558P
Metal ion induced FRET OFF-ON in tren/dansyl-appended rhodamine.
M. H. Lee (2008)
10.1089/ars.2010.3572
Regulation of heart function by endogenous gaseous mediators-crosstalk between nitric oxide and hydrogen sulfide.
Qian-Chen Yong (2011)
10.1021/ja404757s
A reductant-resistant and metal-free fluorescent probe for nitroxyl applicable to living cells.
Kodai Kawai (2013)
10.1016/S0891-5849(00)00247-1
Nitric oxide (NO)-related pharmaceuticals: contemporary approaches to therapeutic NO modulation.
D. Janero (2000)
10.1152/physrev.00014.2008
Positive and negative regulation of insulin signaling by reactive oxygen and nitrogen species.
N. Bashan (2009)
10.1021/ac501263d
Design of a simultaneous target and location-activatable fluorescent probe for visualizing hydrogen sulfide in lysosomes.
S. Yang (2014)
10.1021/ar300273v
FRET-based small-molecule fluorescent probes: rational design and bioimaging applications.
L. Yuan (2013)
10.1039/c5cc08695g
A FRET-based ratiometric two-photon fluorescent probe for dual-channel imaging of nitroxyl in living cells and tissues.
X. Zhu (2016)
10.1523/JNEUROSCI.16-03-01066.1996
The possible role of hydrogen sulfide as an endogenous neuromodulator
K. Abe (1996)
10.1021/ja500315x
A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
Alexandra T. Wrobel (2014)
10.1093/cvr/cvq248
Hydrogen sulfide interacts with nitric oxide in the heart: possible involvement of nitroxyl.
Qian-Chen Yong (2010)
10.1021/acs.analchem.6b00521
Target-Activated Modulation of Dual-Color and Two-Photon Fluorescence of Graphene Quantum Dots for in Vivo Imaging of Hydrogen Peroxide.
Wenjie Zhao (2016)
10.1002/adma.201602939
Simultaneous Near-Infrared and Two-Photon In Vivo Imaging of H2 O2 Using a Ratiometric Fluorescent Probe based on the Unique Oxidative Rearrangement of Oxonium.
Baoli Dong (2016)
10.1016/J.TETLET.2007.01.176
Design of molecular two-photon probes for in vivo imaging. 2H-Benzo[h]chromene-2-one derivatives
H. Kim (2007)
10.1073/pnas.1509277112
Key bioactive reaction products of the NO/H2S interaction are S/N-hybrid species, polysulfides, and nitroxyl
M. Cortese-Krott (2015)
10.1039/c4cc07561g
Visualization of nitroxyl (HNO) in vivo via a lysosome-targetable near-infrared fluorescent probe.
X. Jing (2014)
10.1016/S0300-483X(02)00060-4
Lipoic acid increases glutathione production and enhances the effect of mercury in human cell lines.
B. Hultberg (2002)
10.1039/c4cc01440e
A highly sensitive and reductant-resistant fluorescent probe for nitroxyl in aqueous solution and serum.
Guo-jiang Mao (2014)
10.1039/c4cc03312d
Fluorescent probes for hydrogen sulfide detection and bioimaging.
Fabiao Yu (2014)
10.1111/jcmm.12077
Crosstalk between hydrogen sulfide and nitric oxide in endothelial cells
Zaid Altaany (2013)
10.1073/pnas.1202916109
Hydrogen sulfide and nitric oxide are mutually dependent in the regulation of angiogenesis and endothelium-dependent vasorelaxation
Ciro Coletta (2012)
10.1039/c5sc04415d
Engineering a nanolab for the determination of lysosomal nitric oxide by the rational design of a pH-activatable fluorescent probe† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc04415d
Yinhui Li (2016)
10.1073/pnas.0709010104
A new gaseous signaling molecule emerges: Cardioprotective role of hydrogen sulfide
D. Lefer (2007)
10.1021/ac403226h
Quinoline-based two-photon fluorescent probe for nitric oxide in live cells and tissues.
X. Dong (2014)
10.1038/nchem.1500
Reaction-based small-molecule fluorescent probes for chemoselective bioimaging.
J. Chan (2012)
10.1038/nrd2425
Hydrogen sulphide and its therapeutic potential
C. Szabo (2007)
10.1038/ncomms5381
H2S and NO cooperatively regulate vascular tone by activating a neuroendocrine HNO–TRPA1–CGRP signalling pathway
Mirjam Eberhardt (2014)
10.1021/ac5000015
Two-photon graphene oxide/aptamer nanosensing conjugate for in vitro or in vivo molecular probing.
M. Yi (2014)
10.1039/c1cs15037e
Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species.
Xiaoqiang Chen (2011)
10.1039/c4cc10382c
A two-photon fluorescent turn-on probe for nitroxyl (HNO) and its bioimaging application in living tissues.
K. Zheng (2015)
10.1155/2016/6904327
Interaction of Hydrogen Sulfide with Nitric Oxide in the Cardiovascular System
B. V. Nagpure (2016)
10.1021/am508987v
A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells.
Huatang Zhang (2015)
Solid-phase synthesis provides a modular, lysine-based platform for fluorescent discrimination of nitroxyl and biological thiols
Andrei Loas (2015)
10.1016/j.aca.2015.05.040
Sensitive and rapid detection of endogenous hydrogen sulfide distributing in different mouse viscera via a two-photon fluorescent probe.
Q. Chen (2015)
10.1021/ic302793w
Detection of nitric oxide and nitroxyl with benzoresorufin-based fluorescent sensors.
Ulf-Peter Apfel (2013)
10.1021/ol103077q
Visualization of nitroxyl in living cells by a chelated copper(II) coumarin complex.
Y. Zhou (2011)
10.1039/C5TB02073E
Development of green to near-infrared turn-on fluorescent probes for the multicolour imaging of nitroxyl in living systems.
Baoli Dong (2016)
10.1021/acs.analchem.5b03664
Consecutive Gated Injection-Based Microchip Electrophoresis for Simultaneous Quantitation of Superoxide Anion and Nitric Oxide in Single PC-12 Cells.
L. Li (2016)
10.1038/306174A0
Endothelium-dependent relaxation in rat aorta may be mediated through cyclic GMP-dependent protein phosphorylation
R. Rapoport (1983)
10.1039/c3cc46095a
A FRET-based ratiometric fluorescent and colorimetric probe for the facile detection of organophosphonate nerve agent mimic DCP.
Weimin Xuan (2013)
10.1039/c6cc05254a
A TP-FRET-based two-photon fluorescent probe for ratiometric visualization of endogenous sulfur dioxide derivatives in mitochondria of living cells and tissues.
X. Yang (2016)
10.1016/J.FREERADBIOMED.2012.11.005
Potential biological chemistry of hydrogen sulfide (H2S) with the nitrogen oxides.
S. King (2013)
10.1039/c4cc00980k
A fast-response, highly sensitive and selective fluorescent probe for the ratiometric imaging of nitroxyl in living cells.
C. Liu (2014)
10.1002/ADFM.201603495
Development of a Unique Class of Spiro-Type Two-Photon Functional Fluorescent Dyes and Their Applications for Sensing and Bioimaging
Hua Chen (2016)



This paper is referenced by
10.1016/J.DYEPIG.2019.03.013
Near-infrared fluorescent probe for imaging nitroxyl in living cells and zebrafish model
Tianlin Wang (2019)
10.1016/j.foodchem.2018.02.130
Highly selective and rapidly responsive fluorescent probe for hydrogen sulfide detection in wine.
Hao Wang (2018)
10.1002/BKCS.11321
A Ratiometric Fluorescent Probe for Selective Detection of Hypochlorite Anion
Zhijian Ou (2017)
10.1039/c9cc04060a
A nitroxyl-responsive near-infrared fluorescent chemosensor for visualizing H2S/NO crosstalk in biological systems.
Mingwang Yang (2019)
10.1016/J.TALANTA.2019.120116
Au-Ag alloy/porous-SiO2 core/shell nanoparticle-based surface-enhanced Raman scattering nanoprobe for ratiometric imaging analysis of nitric oxide in living cells.
Lu-Lu Li (2019)
10.1111/1750-3841.14015
A Reaction-Based Novel Fluorescent Probe for Detection of Hydrogen Sulfide and Its Application in Wine.
Hao Wang (2018)
10.1039/c9cs00166b
Rhodols - synthesis, photophysical properties and applications as fluorescent probes.
Yevgen M Poronik (2019)
10.1039/c9nr00880b
A spherical nucleic acid-based two-photon nanoprobe for RNase H activity assay in living cells and tissues.
Ningning Wang (2019)
10.1016/j.freeradbiomed.2018.04.564
Fluorescent probes for the detection of nitroxyl (HNO)
Renata Smulik-Izydorczyk (2018)
10.1016/j.dyepig.2019.108132
G-quadruplex-lighted EVCP for the construction of ratiometric fluorescent sensors: Detection of hydrogen peroxide and oxidase substrate
N. Wang (2020)
10.1039/C8AY02819B
A 6-acetyl-2-naphthol based two-photon fluorescent probe for the selective detection of nitroxyl and imaging in living cells
Pingzhu Zhang (2019)
10.1021/acs.analchem.7b02649
Improved Aromatic Substitution-Rearrangement-Based Ratiometric Fluorescent Cysteine-Specific Probe and Its Application of Real-Time Imaging under Oxidative Stress in Living Zebrafish.
L. He (2017)
10.1016/j.talanta.2020.120884
A bond energy transfer based difunctional fluorescent sensor for Cys and bisulfite.
Fangfang Zhao (2020)
10.1039/d0pp00201a
Dihydropyridine-coumarin-based fluorescent probe for imaging nitric oxide in living cells.
Sufang Ma (2020)
10.1016/j.snb.2020.128211
A two-photon fluorescent probe for HNO rapid visualization in endoplasmic reticulum
Peng Shuxin (2020)
10.1039/c9an01767d
A highly selective ratiometric fluorescent probe based on naphthalimide for detection and imaging of CYP1A1 in living cells and zebrafish.
Hefang Ji (2019)
10.1016/j.jlumin.2019.117018
Facile construction of AIE-based FRET nanoprobe for ratiometric imaging of hypochlorite in live cells
K. Nie (2020)
10.1016/j.biomaterials.2020.119910
Developing a ratiometric two-photon probe with baseline resolved emissions by through band energy transfer strategy: Tracking mitochondrial SO2 during neuroinflammation.
W. Hu (2020)
10.3390/molecules23092241
Salivary Hydrogen Sulfide Measured with a New Highly Sensitive Self-Immolative Coumarin-Based Fluorescent Probe
Ewelina Zaorska (2018)
10.1016/j.saa.2020.118680
A near-infrared fluorescent probe for imaging of nitroxyl in living cells.
Z. Liu (2020)
10.1002/chem.201900246
Donor and Ring-Fusing Engineering for Far-Red to Near-Infrared Triphenylpyrylium Fluorophores with Enhanced Fluorescence Performance for Sensing and Imaging.
Si-Yu Wen (2019)
10.1016/j.aca.2018.05.064
A highly selective and ultrasensitive ratiometric fluorescent probe for peroxynitrite and its two-photon bioimaging applications.
Zuokai Wang (2019)
10.1039/C9AY00285E
A nucleic acid cleavage-based fluorescent probe for the detection of copper ions in pure aqueous solution
Qirong Deng (2019)
10.1002/ADFM.201800515
Au/AgI Dimeric Nanoparticles for Highly Selective and Sensitive Colorimetric Detection of Hydrogen Sulfide
Jingbin Zeng (2018)
10.1016/J.DYEPIG.2020.108889
A fluorescent probe with low-background for visualization of nitroxyl in living cells and zebrafish
Changli Zhang (2021)
10.1021/acs.analchem.7b04211
Ratiometric Detection of Intracellular Lysine and pH with One-Pot Synthesized Dual Emissive Carbon Dots.
W. Song (2017)
10.1002/SLCT.201901803
Metal‐Free Synthesis of Coumarin‐fused Pyrimidines from 4‐Aminocoumarins via Pseudo Four‐component Reaction
C. Jing (2019)
10.1039/c9sc01652j
Fluorescent probes for organelle-targeted bioactive species imaging
Peng Gao (2019)
10.1002/anie.201811257
A Chemiluminescent Probe for HNO Quantification and Real-Time Monitoring in Living Cells.
Weiwei An (2019)
10.1021/acschembio.7b00901
Reaction-Based Fluorescent Probes for the Imaging of Nitroxyl (HNO) in Biological Systems.
Baoli Dong (2018)
10.1021/acs.chemrev.7b00205
Chemical Biology of H2S Signaling through Persulfidation.
M. Filipovic (2018)
10.1039/c8ob02351d
Catalytic dehydrogenative aromatization of cyclohexanones and cyclohexenones.
Xueli Liu (2018)
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