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A Super-Resolution Probe To Monitor HNO Levels In The Endoplasmic Reticulum Of Cells.
F. Ali, Sreejesh Sreedharan, Anila Hoskere Ashoka, H. Saeed, C. Smythe, J. Thomas, A. Das
Published 2017 · Chemistry, Medicine
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Selective detection of nitroxyl (HNO), which has recently been identified as a reactive nitrogen species, is a challenging task. We report a BODIPY-based luminescence ON reagent for detection of HNO in aqueous solution and in live RAW 264.7 cells, based on the soft nucleophilicity of the phosphine oxide functionality toward HNO. The probe shows high selectivity to HNO over other reactive oxygen/nitrogen and sulfur species. Luminescence properties of the BODIPY-based chemodosimetric reagent make it an ideal candidate for use as a reagent for super-resolution structured illumination microscopy. The viability of the reagent for biological in vivo imaging application was also confirmed using Artemia as a model.
This paper references
Metal-based optical probes for live cell imaging of nitroxyl (HNO).
Pablo Rivera-Fuentes (2015)
Formation of free nitric oxide from l-arginine by nitric oxide synthase: direct enhancement of generation by superoxide dismutase.
A. Hobbs (1994)
The emergence of nitroxyl (HNO) as a pharmacological agent.
C. Switzer (2009)
The physiological chemistry and biological activity of nitroxyl (HNO): the neglected, misunderstood, and enigmatic nitrogen oxide.
J. Fukuto (2005)
The chemistry of nitroxyl (HNO) and implications in biology
K. Miranda (2005)
The pharmacology of nitroxyl (HNO) and its therapeutic potential: not just the Janus face of NO.
N. Paolocci (2007)
A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
Alexandra T. Wrobel (2014)
A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells.
Huatang Zhang (2015)
Visualization of nitroxyl (HNO) in vivo via a lysosome-targetable near-infrared fluorescent probe.
X. Jing (2014)
Cytotoxicity of nitroxyl (HNO/NO-) against normal and cancer human cells.
Aleksandra Augustyniak (2013)
Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison
E. Wegel (2016)
Donors of HNO.
K. Miranda (2005)
Reductive phosphine-mediated ligation of nitroxyl (HNO).
J. Reisz (2009)
A biochemical rationale for the discrete behavior of nitroxyl and nitric oxide in the cardiovascular system
K. Miranda (2003)
Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level.
S. Suárez (2013)
A targetable fluorescent probe for imaging exogenous and intracellularly formed nitroxyl in mitochondria in living cells.
Mingguang Ren (2017)
Rapid and selective nitroxyl (HNO) trapping by phosphines: kinetics and new aqueous ligations for HNO detection and quantitation.
J. Reisz (2011)
A fast-response, highly sensitive and selective fluorescent probe for the ratiometric imaging of nitroxyl in living cells.
C. Liu (2014)
A Near-Infrared Fluorescent Probe for Detection of Nitroxyl in Living Cells
P. Liu (2015)
A fluorescent probe for specific detection of cysteine in the lipid dense region of cells.
F. Ali (2015)
Reactivity of C-terminal cysteines with HNO.
G. Keceli (2014)
On the acidity and reactivity of HNO in aqueous solution and biological systems
M. D. Bartberger (2001)
A reductant-resistant and metal-free fluorescent probe for nitroxyl applicable to living cells.
Kodai Kawai (2013)
Live-cell super-resolution imaging with synthetic fluorophores.
S. van de Linde (2012)
Nitroxyl and its anion in aqueous solutions: Spin states, protic equilibria, and reactivities toward oxygen and nitric oxide
V. Shafirovich (2002)
Development of green to near-infrared turn-on fluorescent probes for the multicolour imaging of nitroxyl in living systems.
Baoli Dong (2016)
A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo.
P. Liu (2015)
A bioorthogonal 'turn-on' fluorescent probe for tracking mitochondrial nitroxyl formation.
Kyoung Sunwoo (2017)
Two-photon red-emissive fluorescent probe for imaging nitroxyl (HNO) in living cells and tissues.
Baoli Dong (2017)
Visualization of nitroxyl in living cells by a chelated copper(II) coumarin complex.
Y. Zhou (2011)
Direct detection of nitroxyl in aqueous solution using a tripodal copper(II) BODIPY complex.
Joel Rosenthal (2010)
Positive inotropic and lusitropic effects of HNO/NO− in failing hearts: Independence from β-adrenergic signaling
N. Paolocci (2003)
A two-photon fluorescent turn-on probe for nitroxyl (HNO) and its bioimaging application in living tissues.
K. Zheng (2015)
Nitroxyl anion exerts redox-sensitive positive cardiac inotropy in vivo by calcitonin gene-related peptide signaling
N. Paolocci (2001)
A highly sensitive and reductant-resistant fluorescent probe for nitroxyl in aqueous solution and serum.
Guo-jiang Mao (2014)
The nitroxyl anion (HNO) is a potent dilator of rat coronary vasculature.
J. Favaloro (2007)
Optical imaging probes for biomolecules: an introductory perspective.
J. Thomas (2015)
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M. Xian (2020)
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Xiaolong Zhao (2019)
The reaction between GSNO and H2S: On the generation of NO, HNO and N2O.
M. R. Kumar (2018)
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Mengdi Liu (2019)
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Fluorescent probes for the detection of nitroxyl (HNO)
Renata Smulik-Izydorczyk (2018)
Recent Advances in Fluorescent Probes for Detection of HOCl and HNO
Anila Hoskere Ashoka (2020)
Polysulfide-triggered fluorescent indicator suitable for super-resolution microscopy and application in imaging.
A. A (2018)
A highly specific rhodamine B based turn-on fluorescent probe for nitric oxide and application in living cells
Yuanyuan Liu (2020)
Development of large Stokes shift, near-infrared fluorescence probe for rapid and bioorthogonal imaging of nitroxyl (HNO) in living cells.
Chun-xia Zhang (2019)
Oxygen Sensing, Hypoxia Tracing and in Vivo Imaging with Functional Metalloprobes for the Early Detection of Non-communicable Diseases
V. Mirabello (2018)
A 6-acetyl-2-naphthol based two-photon fluorescent probe for the selective detection of nitroxyl and imaging in living cells
Pingzhu Zhang (2019)
A caged 2-hydroxyethyl luciferin for bioluminescence imaging of nitroxyl in living cells.
Y. Ju (2020)
Revealing the redox status in endoplasmic reticulum by a selenium fluorescence probe.
Shunping Zang (2020)
A fluorescent probe with low-background for visualization of nitroxyl in living cells and zebrafish
Changli Zhang (2021)
A highly sensitive and selective fluorescent probe for nitroxyl based on a naphthalene derivative
Q. Ma (2019)
ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite.
Luling Wu (2018)