Online citations, reference lists, and bibliographies.
← Back to Search

Role Of The Protein Corona Derived From Human Plasma In Cellular Interactions Between Nanoporous Human Serum Albumin Particles And Endothelial Cells.

M. Zyuzin, Y. Yan, R. Hartmann, K. Gause, Moritz Nazarenus, J. Cui, F. Caruso, W. Parak
Published 2017 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The presence of a protein corona on various synthetic nanomaterials has been shown to strongly influence how they interact with cells. However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle-cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that various serum proteins adsorbed on the particles, when exposed to human blood plasma, forming a corona. In human umbilical vein endothelial cells (HUVECs), the corona was shown to decrease particle binding to the cell membrane, increase the residence time of particles in early endosomes, and reduce the amount of internalized particles within the first hours of exposure to particles. These findings reveal important information regarding the mechanisms used by vascular endothelial cells to internalize protein-based particulate materials exposed to blood plasma. The ability to control the cellular recognition of these organic particles is expected to aid the advancement of HSA-based materials for intravenous drug delivery.
This paper references
10.1016/j.biomaterials.2013.11.001
Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles.
Hong Jai Lee (2014)
10.1002/jcb.25061
Caveolae May Enable Albumin to Enter Human Renal Glomerular Endothelial Cells
T. Moriyama (2015)
10.1021/nn3059295
Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge.
Dominik Hühn (2013)
10.1039/c5nr07308a
Combined image guided monitoring the pharmacokinetics of rapamycin loaded human serum albumin nanoparticles with a split luciferase reporter.
Fu Wang (2016)
10.1002/smll.201503232
Influence of Temperature on the Colloidal Stability of Polymer-Coated Gold Nanoparticles in Cell Culture Media.
M. Zyuzin (2016)
10.1016/J.IJPHARM.2007.06.028
Human serum albumin (HSA) nanoparticles: reproducibility of preparation process and kinetics of enzymatic degradation.
K. Langer (2008)
10.3762/bjnano.6.88
Protein corona – from molecular adsorption to physiological complexity
L. Treuel (2015)
10.1021/acs.molpharmaceut.5b00153
Ligand-Modified Human Serum Albumin Nanoparticles for Enhanced Gene Delivery.
Jennifer Look (2015)
10.1016/j.copbio.2017.02.009
Techniques for the experimental investigation of the protein corona.
C. Carrillo-Carrión (2017)
10.1021/nn404481f
Differential roles of the protein corona in the cellular uptake of nanoporous polymer particles by monocyte and macrophage cell lines.
Yan Yan (2013)
10.1021/jacs.6b11302
Modulated Fragmentation of Proapoptotic Peptide Nanoparticles Regulates Cytotoxicity.
T. Suma (2017)
10.1021/acsnano.5b00393
Physicochemical and immunological assessment of engineered pure protein particles with different redox states.
K. Gause (2015)
10.1111/j.1365-2818.1993.tb03313.x
Measurement of co‐localization of objects in dual‐colour confocal images
E. M. Manders (1993)
10.1021/nn401800u
Particles on the move: intracellular trafficking and asymmetric mitotic partitioning of nanoporous polymer particles.
Yan Yan (2013)
10.1002/ADFM.201401664
Back to Basics: Exploiting the Innate Physico‐chemical Characteristics of Nanomaterials for Biomedical Applications
C. Y. Tay (2014)
10.3109/02652049409040455
Phagocytosis and degradation of human serum albumin microspheres and nanoparticles in human macrophages.
V. Schaefer (1994)
10.1016/j.biocel.2015.12.015
Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance.
Jonas Hühn (2016)
10.1038/nnano.2012.207
Biomolecular coronas provide the biological identity of nanosized materials.
M. Monopoli (2012)
10.1083/jcb.200302028
Caveolae/raft-dependent endocytosis
I. Nabi (2003)
10.1039/c6nr03369e
Synthesis and functionalization of monodisperse near-ultraviolet and visible excitable multifunctional Eu(3+), Bi(3+):REVO4 nanophosphors for bioimaging and biosensing applications.
A. Escudero (2016)
10.1021/nn204951s
Reversible versus irreversible binding of transferrin to polystyrene nanoparticles: soft and hard corona.
S. Milani (2012)
10.1098/rsif.2009.0272.focus
Quantitative analysis of the protein corona on FePt nanoparticles formed by transferrin binding
X. Jiang (2009)
10.1016/J.BBRC.2004.04.067
Incorporation of biodegradable nanoparticles into human airway epithelium cells-in vitro study of the suitability as a vehicle for drug or gene delivery in pulmonary diseases.
M. Brzoska (2004)
10.2147/IJN.S10443
Human serum albumin nanoparticles as an efficient noscapine drug delivery system for potential use in breast cancer: preparation and in vitro analysis
S. Sebak (2010)
10.1021/acs.accounts.6b00088
Nanoengineered Templated Polymer Particles: Navigating the Biological Realm.
J. Cui (2016)
10.1021/ACS.LANGMUIR.6B01634
A Framework to Account for Sedimentation and Diffusion in Particle-Cell Interactions.
J. Cui (2016)
10.1002/ADFM.201201191
Templated Assembly of pH-Labile Polymer-Drug Particles for Intracellular Drug Delivery
J. Cui (2012)
10.1016/j.biomaterials.2013.08.085
Self-crosslinked human serum albumin nanocarriers for systemic delivery of polymerized siRNA to tumors.
Sohee Son (2013)
10.1021/acs.bioconjchem.6b00657
Comprehensive and Systematic Analysis of the Immunocompatibility of Polyelectrolyte Capsules.
M. Zyuzin (2017)
10.1016/j.scitotenv.2016.05.213
Quantitative uptake of colloidal particles by cell cultures.
Neus Feliu (2016)
10.3109/02652041003681406
Adsorption of obidoxime onto human serum albumin nanoparticles: Drug loading, particle size and drug release
Jürgen Kufleitner (2010)
10.1038/nnano.2012.237
Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface.
A. Salvati (2013)
10.1002/adma.201300981
Mechanically tunable, self-adjuvanting nanoengineered polypeptide particles.
J. Cui (2013)
10.1038/nrm2122
The multiple faces of caveolae
R. Parton (2007)
10.3762/bjnano.5.161
In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?
Moritz Nazarenus (2014)
10.1034/j.1600-0854.2003.00128.x
Lipid Rafts and Caveolae as Portals for Endocytosis: New Insights and Common Mechanisms
R. Parton (2003)
10.1007/s00418-001-0367-x
Vesicle formation and trafficking in endothelial cells and regulation of endothelial barrier function
Richard D. Minshall (2001)
10.1074/JBC.C100613200
Caveolae-deficient Endothelial Cells Show Defects in the Uptake and Transport of Albumin in Vivo *
W. Schubert (2001)
10.1016/j.addr.2007.08.044
Protein nanoparticles as drug carriers in clinical medicine.
M. Hawkins (2008)
10.1002/smll.201000528
Quantitative evaluation of cellular uptake and trafficking of plain and polyethylene glycol-coated gold nanoparticles.
C. Brandenberger (2010)
10.1039/c5cs00217f
The nanoparticle biomolecule corona: lessons learned - challenge accepted?
D. Docter (2015)
10.1021/nn306032k
Multiple internalization pathways of polyelectrolyte multilayer capsules into mammalian cells.
Lena Kastl (2013)
10.1021/ACS.CHEMMATER.6B04738
Selected Standard Protocols for the Synthesis, Phase Transfer, and Characterization of Inorganic Colloidal Nanoparticles
Jonas Hühn (2017)
10.1002/anie.201409693
Stiffness-dependent in vitro uptake and lysosomal acidification of colloidal particles.
R. Hartmann (2015)
10.1021/mp500394v
Doxorubicin-loaded glycyrrhetinic acid modified recombinant human serum albumin nanoparticles for targeting liver tumor chemotherapy.
Wen-Wen Qi (2015)
10.1016/J.CIS.2007.04.021
The nanoparticle-protein complex as a biological entity; a complex fluids and surface science challenge for the 21st century.
I. Lynch (2007)
10.1021/nn5061578
Engineering poly(ethylene glycol) particles for improved biodistribution.
J. Cui (2015)
10.3109/02652048.2010.501395
Incorporation of obidoxime into human serum albumin nanoparticles: optimisation of preparation parameters for the development of a stable formulation
Jürgen Kufleitner (2010)
10.1016/J.NANO.2007.10.021
Nanoparticle albumin bound (nab) technology: targeting tumors through the endothelial gp60 receptor and SPARC
N. Desai (2007)
10.2217/nnm.15.163
Nanomedicine delivery: does protein corona route to the target or off road?
D. Maiolo (2015)
10.1002/smll.200800596
A novel flow-cytometry-based assay for cellular uptake studies of polyelectrolyte microcapsules.
Maximilian Semmling (2008)
10.1016/J.JCONREL.2004.01.029
Tumour cell delivery of antisense oligonuclceotides by human serum albumin nanoparticles.
Heidrun Wartlick (2004)
10.1021/nn300223w
Effects of the presence or absence of a protein corona on silica nanoparticle uptake and impact on cells.
A. Leśniak (2012)
10.1016/S1359-6349(10)70002-1
Nanoparticle albumin-bound (nab™)-paclitaxel: improving efficacy and tolerability by targeted drug delivery in metastatic breast cancer
J. Cortés (2010)
10.1039/C3MH00106G
Protein corona formation around nanoparticles – from the past to the future
P. Pino (2014)
10.1002/btm2.10010
Engineering of nanoparticle size via electrohydrodynamic jetting
S. Rahmani (2016)



This paper is referenced by
10.1038/s41598-020-75685-3
A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM
Sandeep Sharma (2020)
cRole of the protein corona derived from human plasma in cellular interactions between nanoporous human serum albumin particles and endothelial cells
M. Zyuzin (2017)
10.1039/c9na00537d
Isolation methods for particle protein corona complexes from protein-rich matrices
Linda Böhmert (2020)
10.1515/nanoph-2019-0423
Optically responsive delivery platforms: from the design considerations to biomedical applications
I. Koryakina (2020)
10.1016/j.ijbiomac.2020.03.118
Dynamic molecular exchange and conformational transitions of alpha-synuclein at the nano-bio interface.
Roberto Tira (2020)
10.1039/c8bm00128f
Detailed investigation on how the protein corona modulates the physicochemical properties and gene delivery of polyethylenimine (PEI) polyplexes.
Dingcheng Zhu (2018)
10.1002/smll.202002861
Cellular Interactions of Liposomes and PISA Nanoparticles during Human Blood Flow in a Microvascular Network
Mai N. Vu (2020)
10.1016/j.ijpharm.2018.10.011
The impact of protein corona on the behavior and targeting capability of nanoparticle‐based delivery system
W. Xiao (2018)
10.1155/2019/8036863
Interaction of Mitoxantrone-Loaded Cholesterol Modified Pullulan Nanoparticles with Human Serum Albumin and Effect on Drug Release
Li-Ming Yuan (2019)
10.2147/IJN.S220082
A Protein Corona Adsorbed to a Bacterial Magnetosome Affects Its Cellular Uptake
W. Lai (2020)
10.1186/s11671-018-2457-x
Dependence of Nanoparticle Toxicity on Their Physical and Chemical Properties
A. Sukhanova (2018)
10.1016/j.addr.2019.02.008
Nanoformulation properties, characterization, and behavior in complex biological matrices: Challenges and opportunities for brain-targeted drug delivery applications and enhanced translational potential.
Vibhuti Agrahari (2019)
10.1002/ANGE.201906303
Analyse quantitativer Partikelaufnahme von Zellen über verschiedene Messmethoden
Sumaira Ashraf (2020)
10.1038/s41565-018-0171-6
Pre-adsorption of antibodies enables targeting of nanocarriers despite a biomolecular corona
Manuel Tonigold (2018)
10.1039/c9bm00926d
Biomimetic drug delivery platforms based on mesenchymal stem cells impregnated with light-responsive submicron sized carriers.
A. Muslimov (2019)
10.1002/anie.201906303
Quantitative Particle Uptake by Cells as Analyzed by Different Methods
Sumaira Ashraf (2019)
Semantic Scholar Logo Some data provided by SemanticScholar