← Back to Search
Bioreduction Of Chromate In A Methane-Based Membrane Biofilm Reactor.
Chun-Yu Lai, L. Zhong, Y. Zhang, Jiaxian Chen, Li-lian Wen, Ling-Dong Shi, Y. Sun, Fang Ma, B. Rittmann, Chen Zhou, Y. Tang, P. Zheng, He-Ping Zhao
Published 2016 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
For the first time, we demonstrate chromate (Cr(VI)) bioreduction using methane (CH4) as the sole electron donor in a membrane biofilm reactor (MBfR). The experiments were divided into five stages lasting a total of 90 days, and each stage achieved a steady state for at least 15 days. Due to continued acclimation of the microbial community, the Cr(VI)-reducing capacity of the biofilm kept increasing. Cr(VI) removal at the end of the 90-day test reached 95% at an influent Cr(VI) concentration of 3 mg Cr/L and a surface loading of 0.37g of Cr m(-2) day(-1). Meiothermus (Deinococci), a potential Cr(VI)-reducing bacterium, was negligible in the inoculum but dominated the MBfR biofilm after Cr(VI) was added to the reactor, while Methylosinus, a type II methanotrophs, represented 11%-21% of the total bacterial DNA in the biofilm. Synergy within a microbial consortia likely was responsible for Cr(VI) reduction based on CH4 oxidation. In the synergy, methanotrophs fermented CH4 to produce metabolic intermediates that were used by the Cr(VI)-reducing bacteria as electron donors. Solid Cr(III) was the main product, accounting for more than 88% of the reduced Cr in most cases. Transmission electron microscope (TEM) and energy dispersive X-ray (EDS) analysis showed that Cr(III) accumulated inside and outside of some bacterial cells, implying that different Cr(VI)-reducing mechanisms were involved.
This paper references
Thermus ruber (Loginova et
T. silvanus Tenreiro (1984)
Complete perchlorate reduction using methane as the sole electron donor and carbon source.
Y. Luo (2015)
Microscopic investigations of the Cr(VI) uptake mechanism of living Ochrobactrum anthropi.
B. Li (2008)
Dissimilatory Metal Reduction by the Facultative Anaerobe Pantoea agglomerans SP1
C. A. Francis (2000)
Chromium(VI) resistance and removal by Acinetobacter haemolyticus
Quek Hsiao Pei (2009)
Highly efficient methane biocatalysis revealed in a methanotrophic bacterium.
M. Kalyuzhnaya (2013)
Bioremediation of metal contamination.
D. Lovley (1997)
Comparison of in vitro Cr(VI) reduction by CFEs of chromate resistant bacteria isolated from chromate contaminated soil.
A. Sarangi (2008)
Nitrate shaped the selenate-reducing microbial community in a hydrogen-based biofilm reactor.
Chun-Yu Lai (2014)
R. S. Hanson (1996)
Simultaneous bioreduction of nitrate and chromate using sulfur-based mixotrophic denitrification process.
E. Şahinkaya (2013)
Chromate efflux by means of the ChrA chromate resistance protein from Pseudomonas aeruginosa.
A. H. Álvarez (1999)
Interaction of ABC transport proteins with toxic metals at the level of gene and transport activity in the PLHC-1 fish cell line.
C. Della Torre (2012)
Purification to Homogeneity and Characterization of a Novel Pseudomonas putida Chromate Reductase
C. H. Park (2000)
Electron energy loss spectroscopy techniques for the study of microbial chromium(VI) reduction.
T. Daulton (2002)
Formation of soluble organo-chromium(III) complexes after chromate reduction in the presence of cellular organics.
G. Puzon (2005)
Global Molecular and Morphological Effects of 24-Hour Chromium(VI) Exposure on Shewanella oneidensis MR-1
K. Chourey (2006)
Hydrogen permeability of the hollow fibers used in H2-based membrane biofilm reactors
Y. Tang (2012)
Comparative metagenomics of microbial communities inhabiting deep-sea hydrothermal vent chimneys with contrasting chemistries
W. Xie (2011)
Analysis of Novel Soluble Chromate and Uranyl Reductases and Generation of an Improved Enzyme by Directed Evolution
Y. Barak (2006)
Mechanisms of chromium toxicity, carcinogenicity and allergenicity: Review of the literature from 1985 to 2000
A. Dayan (2001)
Aerobic Cr(VI) reduction by Thermus scotoductus strain SA‐01
D. Opperman (2007)
Investigation of Cr(VI) reduction and Cr(III) immobilization mechanism by planktonic cells and biofilms of Bacillus subtilis ATCC-6633.
X. Pan (2014)
Isolation and Characterization of an Enterobacter cloacae Strain That Reduces Hexavalent Chromium under Anaerobic Conditions.
P. C. Wang (1989)
Processes affecting the remediation of chromium-contaminated sites.
C. Palmer (1991)
Interactions between perchlorate and nitrate reductions in the biofilm of a hydrogen-based membrane biofilm reactor.
He-Ping Zhao (2011)
Chromium intake, absorption and excretion of subjects consuming self-selected diets.
R. Anderson (1985)
A novel chromate reductase from Thermus scotoductus SA-01 related to old yellow enzyme.
D. Opperman (2008)
Intracytoplasmic membranes in oxygen-limited chemostat cultures of Methylosinus trichosporium OB3b: Biocatalytic implications of physiologically balanced growth
D. Scott (2004)
Bio-reduction of soluble chromate using a hydrogen-based membrane biofilm reactor.
J. Chung (2006)
Effect of chromate action on morphology of basalt-inhabiting bacteria
Z. Lin (2006)
Chromium occurrence in the environment and methods of its speciation.
J. Kotas (2000)
Element fi xation in soil
J. Dragun (1991)
Hydrogen - Permeation coefficients of the fibers used in H 2 - based membrane biofilm reactors
Y. N. Tang (2012)
Chromium(VI) bioremoval by Pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination.
N. M. Doğan (2011)
Diet and dental health.
Shaw Jh (1985)
In situ stabilization of chromium(VI) in polluted soils using organic ligands: the role of galacturonic, glucuronic and alginic acids.
C. Kantar (2008)
Mechanism of chromate reduction by the Escherichia coli protein, NfsA, and the role of different chromate reductases in minimizing oxidative stress during chromate reduction.
D. Ackerley (2004)
Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater.
A. Zahoor (2009)
Chromate resistance and reduction in Pseudomonas fluorescens strain LB300
L. Bopp (2004)
Effect of Carbon and Energy Source on Bacterial Chromate Reduction
W. A. Smith (2002)
Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: a review.
H. Thatoi (2014)
Nitrogen removal from wastewater by coupling anammox and methane-dependent denitrification in a membrane biofilm reactor.
Ying Shi (2013)
Calorimetric study on the decomposition of hydroxylamine in the presence of transition metals.
M. Kumasaki (2004)
Chromium reduction in Pseudomonas putida.
Y. Ishibashi (1990)
Interactions between metal ions and capsular polymers
G. Geesey (1989)
Mechanism of hexavalent chromium detoxification by microorganisms and bioremediation application potential: A review
K. H. Cheung (2007)
Membrane-bound respiratory system of Enterobacter cloacae strain HO1 grown anaerobically with chromate.
P. C. Wang (1991)
Effect of Chromium and Organic Acids on Microbial Growth and Exopolymeric Substance Production by Pseudomonas Bacteria
N. M. Doğan (2014)
Remediation of chromium(VI) by a methane-oxidizing bacterium.
Abubakr Al Hasin (2010)
Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences
M. Langille (2013)
chilarophilus comb. nov., respectively, and emendation of the genus Thermus
Influence of heavy metals on methane oxidation in tropical rice soils.
S. Mohanty (2000)
Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent
S. Srivastava (2006)
Occurrences, uses, and properties of chromium.
J. Barnhart (1997)
Speciation Analysis of Chromium Using Cryptand Ethers
E. A. García (1997)
Denitrification with methane as external carbon source.
O. Modin (2007)
This paper is referenced by
NGS-based characterization of microbial diversity and functional profiling of solid tannery waste metagenomes.
S. Verma (2020)
Response of soil microbial communities and microbial interactions to long-term heavy metal contamination.
X. Li (2017)
Effects of redox potential on soil cadmium solubility: Insight into microbial community.
Delong Meng (2019)
Effects of sludge lysate for Cr(VI) bioreduction and analysis of bioaugmentation mechanism of sludge humic acid
Hongling Chen (2019)
Resilient biological hexavalent chromium removal with a two‐stage, fixed‐bed biotreatment system
G. Upadhyaya (2019)
Oxygen exposure deprives antimonate-reducing capability of a methane fed biofilm.
Chun-Yu Lai (2018)
Formation of nanoscale Te0 and its effect on TeO32- reduction in CH4-based membrane biofilm reactor.
Ling-Dong Shi (2019)
The role of natural Fe(II)-bearing minerals in chemoautotrophic chromium (VI) bio-reduction in groundwater.
Jian-ping Lu (2019)
Bioremediation of selenium species in solution bymethanotrophic bacteria
Abdurrahman S. Eswyah (2018)
A mixed consortium of methanotrophic archaea and bacteria boosts methane-dependent selenate reduction.
Ling-Dong Shi (2020)
Syntrophic metabolism of phenol in the anodic degradation within a Phenol-Cr(VI) coupled microbial electrolysis cell.
Cheng Sun (2020)
Simultaneous sorption and reduction of Cr(VI) in aquatic system by microbial extracellular polymeric substances from Klebsiella sp. J1
W. Wei (2018)
Detoxification and immobilization of chromite ore processing residue using the alkali-activated cementitious materials mixed with ascorbic acid.
M. Xia (2020)
Enhanced removal of Cr(VI) in the Fe(III)/natural polyphenols system: role of the in situ generated Fe(II).
Yu Wang (2019)
Perchlorate bioreduction linked to methane oxidation in a membrane biofilm reactor: Performance and microbial community structure.
T. Xie (2018)
Enhancement of the biodegradability of activated sludge by the electric-coagulation multistage A/O membrane bioreactor treating low C/N industrial wastewater
Guangsheng Qian (2017)
Metagenomic Evidence for a Methylocystis Species Capable of Bioremediation of Diverse Heavy Metals
Ling-Dong Shi (2019)
Microbial transformations of selenite by methane-oxidizing bacteria
Abdurrahman S. Eswayah (2017)
Microbial chromate reduction coupled with anaerobic oxidation of methane in a membrane biofilm reactor.
Jing-Huan Luo (2019)
Methane oxidation coupled to vanadate reduction in a membrane biofilm batch reactor under hypoxic condition
Zheng Wang (2019)
Nitrate effects on chromate reduction in a methane-based biofilm.
L. Zhong (2017)
Enhanced Microbial Chromate Reduction Using Hydrogen and Methane as Joint Electron Donors.
Chao He (2020)
Hydrogenotrophic Microbial Reduction of Oxyanions With the Membrane Biofilm Reactor
Chen Zhou (2019)
Degradation of organic pollutants by anaerobic methane-oxidizing microorganisms using methyl orange as example.
L. Fu (2019)
Heavy metal reduction coupled to methane oxidation：mechanisms, recent advances and future perspectives
Cheng-Cheng Dang (2020)
Chromium isotope fractionation during Cr(VI) reduction in a methane-based hollow-fiber membrane biofilm reactor.
Yong-ze Lu (2018)
Characterization of Product and Potential Mechanism of Cr(VI) Reduction by Anaerobic Activated Sludge in a Sequencing Batch Reactor
Ruofei Jin (2017)
Hypoxic methane oxidation coupled to denitrification in a membrane biofilm
Wael Alrashed (2018)
Managing microbial communities in membrane biofilm reactors
A. Ontiveros-Valencia (2018)
The effect of light intensity and shear stress on microbial biostabilization and the community composition of natural biofilms
H. Schmidt (2018)
High-rate anaerobic decolorization of methyl orange from synthetic azo dye wastewater in a methane-based hollow fiber membrane bioreactor.
Ya-Nan Bai (2019)
Enhancement of synchronous bio-reductions of vanadium (V) and chromium (VI) by mixed anaerobic culture.
S. Wang (2018)See more