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High-Performance Three-Dimensional Tubular Nanomembrane Sensor For DNA Detection.

M. Medina-Sánchez, B. Ibarlucea, Nicolás Pérez, D. Karnaushenko, Sonja M. Weiz, L. Baraban, G. Cuniberti, O. Schmidt
Published 2016 · Materials Science, Medicine

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We report an ultrasensitive label-free DNA biosensor with fully on-chip integrated rolled-up nanomembrane electrodes. The hybridization of complementary DNA strands (avian influenza virus subtype H1N1) is selectively detected down to attomolar concentrations, an unprecedented level for miniaturized sensors without amplification. Impedimetric DNA detection with such a rolled-up biosensor shows 4 orders of magnitude sensitivity improvement over its planar counterpart. Furthermore, it is observed that the impedance response of the proposed device is contrary to the expected behavior due to its particular geometry. To further investigate this difference, a thorough model analysis of the measured signal and the electric field calculation is performed, revealing enhanced electron hopping/tunneling along the DNA chains due to an enriched electric field inside the tube. Likewise, conformational changes of DNA might also contribute to this effect. Accordingly, these highly integrated three-dimensional sensors provide a tool to study electrical properties of DNA under versatile experimental conditions and open a new avenue for novel biosensing applications (i.e., for protein, enzyme detection, or monitoring of cell behavior under in vivo like conditions).
This paper references
10.1039/c2lc40275k
Lab-in-a-tube: on-chip integration of glass optofluidic ring resonators for label-free sensing applications.
S. Harazim (2012)
10.1002/ELAN.200804268
DNA Stabilization and Hybridization Detection on Porous Silicon Surface by EIS and Total Reflection FT‐IR Spectroscopy
V. Vamvakaki (2008)
Anal. Chem
H.-F Cui
10.1007/S10404-012-1068-6
A novel method to construct 3D electrodes at the sidewall of microfluidic channel
Shunbo Li (2013)
10.1016/j.ab.2015.09.011
[Cu(phen)2](2+) acts as electrochemical indicator and anchor to immobilize probe DNA in electrochemical DNA biosensor.
L. Yang (2016)
Anal. Chem
G L Long (1983)
10.1021/bi702363u
Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations.
R. Owczarzy (2008)
10.1016/j.bios.2014.09.069
A controlled microfluidic electrochemical lab-on-a-chip for label-free diffusion-restricted DNA hybridization analysis.
H. Ben-Yoav (2015)
10.1021/ac980656z
Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).
D. C. Duffy (1998)
10.1021/MA8010087
Conformational Preconditioning by Electrophoresis of DNA through a Finite Obstacle Array
A. Balducci (2008)
10.1002/anie.201208357
Naturally rolled-up C/Si/C trilayer nanomembranes as stable anodes for lithium-ion batteries with remarkable cycling performance.
Junwen Deng (2013)
10.1007/s10008-015-3071-9
Poly(indole-5-carboxylic acid)-functionalized ZnO nanocomposite for electrochemical DNA hybridization detection
W. Zhang (2015)
10.1021/ac500618v
Multicolor Fluorescent Biosensor for Multiplexed Detection of DNA
Rong Hu (2014)
10.1016/j.aca.2013.03.030
A sensitive and label-free impedimetric biosensor based on an adjunct probe.
X. Zhang (2013)
10.1103/REVMODPHYS.76.195
Colloquium: The quest for high-conductance DNA
R. Endres (2004)
10.1039/c1an15061h
A novel fluorescent biosensor for sequence-specific recognition of double-stranded DNA with the platform of graphene oxide.
Chengke Wu (2011)
Lab Chip
S M Harazim
Sens. Actuators, B
S Bizid (2016)
10.1016/j.aca.2013.09.024
Signal-on impedimetric electrochemical DNA sensor using dithiothreitol modified gold nanoparticle tag for highly sensitive DNA detection.
C. Wang (2013)
10.1093/NAR/GNH003
Impedance-based detection of DNA sequences using a silicon transducer with PNA as the probe layer.
A. Macanovic (2004)
10.1021/am4038245
Patterned biochemical functionalization improves aptamer-based detection of unlabeled thrombin in a sandwich assay.
L. Römhildt (2013)
10.1088/0957-4484/19/37/375202
Modelling the effect of structure and base sequence on DNA molecular electronics.
M. Ramos (2008)
10.1073/PNAS.96.21.11713
Long-range charge hopping in DNA.
M. Bixon (1999)
N. B. Anal. Chim. Acta
X Y Zhang (2013)
10.1101/GR.6.10.986
Real time quantitative PCR.
C. A. Heid (1996)
Nat. Chem
D Muren
10.1002/adma.201503696
Biomimetic Microelectronics for Regenerative Neuronal Cuff Implants.
D. Karnaushenko (2015)
10.1002/AENM.201470043
Energy Storage: Large‐Area Rolled‐Up Nanomembrane Capacitor Arrays for Electrostatic Energy Storage (Adv. Energy Mater. 9/2014)
R. Sharma (2014)
C. Anal. Biochem
L Yang (2016)
10.4172/2155-9872.S7-016
Fundamentals and Applications of Impedimetric and Redox Capacitive Biosensors
A. Santos (2014)
10.1039/c2lc40063d
Nanomaterials and lab-on-a-chip technologies.
M. Medina-Sánchez (2012)
M. Anal. Chem
(1998)
10.1002/(SICI)1521-3773(19990401)38:7<941::AID-ANIE941>3.0.CO;2-7
Long-Range Electron Transfer through DNA Films.
S. Kelley (1999)
Genome Res
C Heid (1996)
10.1371/journal.pone.0035253
DNA Aptamers against the Lup an 1 Food Allergen
P. Nadal (2012)
ACS Appl. Mater. Interfaces
L Rö Mhildt
10.1021/nl303887b
Rolled-up nanomembranes as compact 3D architectures for field effect transistors and fluidic sensing applications.
Daniel Grimm (2013)
Chem. Commun
Y Hu (2011)
Nano Lett
10.1021/nn306024a
Quantifying Thiol Ligand Density of Self-Assembled Monolayers on Gold Nanoparticles by Inductively Coupled Plasma–Mass Spectrometry
Helmut Hinterwirth (2013)
10.1086/651132
Severity of Pneumonia Due to New H1N1 Influenza Virus in Ferrets Is Intermediate between That Due to Seasonal H1N1 Virus and Highly Pathogenic Avian Influenza H5N1 Virus
J. van den Brand (2010)
J. A. F. Anal. Methods
W K T Coltro
10.1007/s100190100124
Nanotechnology
J. Gilman (2001)
1192−1199. (8) Bonanni, A.; Pividori, M. I.; del Valle, M. Analyst 2010
T G Drummond (2003)
PLoS One
(2012)
Lab Chip
D Berdat (2008)
10.1038/nbt873
Electrochemical DNA sensors
T. G. Drummond (2003)
10.1016/j.jviromet.2014.01.024
Analysis of sensitivity and rapid hybridization of a multiplexed Microbial Detection Microarray.
J. Thissen (2014)
10.1038/35065525
Nanotechnology: Thin solid films roll up into nanotubes
O. Schmidt (2001)
10.1002/adma.201203458
Highly conductive and strain-released hybrid multilayer Ge/Ti nanomembranes with enhanced lithium-ion-storage capability.
C. Yan (2013)
10.1093/NAR/24.15.3031
Covalent attachment of synthetic DNA to self-assembled monolayer films.
L. Chrisey (1996)
J. Infect. Dis
J M A Van Den Brand (2010)
Biosens. Bioelectron
R.-M Kong (2014)
10.1109/14.42159
Assessing the moisture barrier properties of polymeric coatings using electrical and electrochemical methods
S. R. Taylor (1989)
J. Solid State Electrochem Talanta
W Zhang (2015)
Adv. Mater
B Koch (2015)
10.1021/ac501800q
Impedimetric DNA detection--steps forward to sensorial application.
M. Riedel (2014)
10.1038/ncomms3689
Attomolar DNA detection with chiral nanorod assemblies
W. Ma (2013)
J. Anal. Bioanal. Tech
A Santos
J. Micromech. Microeng
Y Yu
10.1002/adhm.201500128
Light Weight and Flexible High-Performance Diagnostic Platform.
D. Karnaushenko (2015)
10.1016/j.talanta.2015.01.035
Simple and label-free detection of DNA hybridization on a modified graphene nanosheets electrode.
A. Benvidi (2015)
10.1039/b712609c
Label-free detection of DNA with interdigitated micro-electrodes in a fluidic cell.
D. Berdat (2008)
Rev. Mod. Phys
R G Endres (2004)
10.1039/C1AY05364G
Capacitively coupled contactless conductivity detection on microfluidic systems—ten years of development
W. K. T. Coltro (2012)
10.1016/S0166-0934(96)02147-7
J. Virol. Methods
D. Gor (1996)
ACS Nano
H Hinterwirth (2013)
10.1021/AC00295A044
Measurements within the diffusion layer using a microelectrode probe
R. C. Engstrom (1986)
10.1038/AM.2015.53
Compact helical antenna for smart implant applications
D. Karnaushenko (2015)
Lab Chip
K Sugioka (2014)
844−848. (57) Bixon
R Engstrom (1986)
Anal. Biochem
J V Staros (1986)
10.1002/ADMI.201500700
Synthesis of Thin‐Layered Molybdenum Disulfide‐Based Polyaniline Nanointerfaces for Enhanced Direct Electrochemical DNA Detection
Tao Yang (2016)
10.1021/AC00258A001
Limit of detection. A closer look at the IUPAC definition
G. Long (1983)
10.1021/nl500795k
Ultracompact Three-Dimensional Tubular Conductivity Microsensors for Ionic and Biosensing Applications
C. Martinez-Cisneros (2014)
Nucleic Acids Res
(2004)
10.1016/j.medmal.2012.07.017
DNA microarrays for the diagnosis of infectious diseases
E. Donatin (2012)
10.1021/nn505825z
Ionic conductivity, structural deformation, and programmable anisotropy of DNA origami in electric field.
Chen-Yu Li (2015)
Lab Chip
M Medina-Sásánchez (1932)
10.1021/nl1036148
Lab-in-a-tube: detection of individual mouse cells for analysis in flexible split-wall microtube resonator sensors.
E. Smith (2011)
Nat. Chem
L Xiang
Angew. Chem., Int. Ed
S O Kelley (1999)
10.1016/J.SNB.2015.11.137
Investigations of poly(p-phenylene) modified with ferrocene and their application in electrochemical DNA sensing
S. Bizid (2016)
10.1088/0960-1317/24/1/015020
Parallel-plate lab-on-a-chip based on digital microfluidics for on-chip electrochemical analysis
Yuhua Yu (2014)
10.1021/la2004694
Enhancing the fluorescence intensity of DNA microarrays by using cationic surfactants.
Siok Lian Lai (2011)
10.1016/0003-2697(86)90176-4
Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions.
J. V. Staros (1986)
10.1016/j.bios.2010.07.108
DNA microarrays for hybridization detection by surface plasmon resonance spectroscopy.
A. Kick (2010)
10.1039/b820557b
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications.
D. Mark (2010)
10.1016/j.talanta.2015.10.043
Comparison of impedimetric detection of DNA hybridization on the various biosensors based on modified glassy carbon electrodes with PANHS and nanomaterials of RGO and MWCNTs.
A. Benvidi (2016)
10.1038/nchem.982
DNA charge transport over 34 nm.
J. Slinker (2011)
10.1002/ELPS.200600113
Fabrication and characterization of semicircular detection electrodes for contactless conductivity detector – CE microchips
Chia-Yen Lee (2006)
10.1529/biophysj.105.064014
Electronic transport in DNA.
D. Klotsa (2005)
疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
宁北芳 (2005)
10.1016/j.bios.2013.11.041
A label-free electrochemical biosensor for highly sensitive and selective detection of DNA via a dual-amplified strategy.
Rongmei Kong (2014)
10.6013/jbrewsocjapan1988.86.425
基礎講座 電気泳動(Electrophoresis)
大房 健 (2005)
Adv. Mater. Interfaces C. Anal. Chim. Acta
T Yang (2013)
10.1038/nchem.2183
Intermediate tunnelling-hopping regime in DNA charge transport.
L. Xiang (2015)
Lab Chip
E J Smith (2012)
Nucleic Acids Res
L Chrisey (1996)
Electr. Insul. Nat. Commun. Biosens. Bioelectron
S R Taylor (1989)
10.1039/c4lc00548a
Femtosecond laser 3D micromachining: a powerful tool for the fabrication of microfluidic, optofluidic, and electrofluidic devices based on glass.
K. Sugioka (2014)
J. Phys. Chem. B
H Gu (2005)
10.1039/c2lc21175k
Lab-in-a-tube: ultracompact components for on-chip capture and detection of individual micro-/nanoorganisms.
E. Smith (2012)
10.1002/pro.5560030516
Macromolecules
C. Tanford (1948)
10.1073/pnas.1105547108
Compression and self-entanglement of single DNA molecules under uniform electric field
J. Tang (2011)
10.1021/ac504206n
Hairpin DNA as a biobarcode modified on gold nanoparticles for electrochemical DNA detection.
Hui-Fang Cui (2015)
10.1016/j.bios.2012.12.044
Real-time, in situ DNA hybridization detection with attomolar sensitivity without amplification using (pb(Mg1/3Nb2/3)O3)0.65-(PbTiO3)0.35 piezoelectric plate sensors.
W. Wu (2013)
10.1021/JP050625P
Electrochemical impedance sensing of DNA hybridization on conducting polymer film-modified diamond.
Huiru Gu (2005)
Biophys. J
D Klotsa (2005)
Mertig, M. Biosens. Bioelectron
A Kick (2010)
10.3390/s140609505
A Compact Microelectrode Array Chip with Multiple Measuring Sites for Electrochemical Applications
M. Dimaki (2014)
10.1038/nature08157
Emergence and pandemic potential of swine-origin H1N1 influenza virus
G. Neumann (2009)
10.1136/bmj.1.4959.155-a
Biochemistry
F. G. Young (1955)
10.1039/c0cc04514d
Label-free electrochemical impedance sensing of DNA hybridization based on functionalized graphene sheets.
Yuwei Hu (2011)
10.1039/c000532k
Impedimetric detection of influenza A (H1N1) DNA sequence using carbon nanotubes platform and gold nanoparticles amplification.
A. Bonanni (2010)
10.1016/j.otohns.2009.05.016
Nature
R. Rosenfeld (2009)



This paper is referenced by
10.1016/j.bios.2019.03.055
A review of microfabricated electrochemical biosensors for DNA detection.
E. Blair (2019)
10.1038/AM.2015.53
Compact helical antenna for smart implant applications
D. Karnaushenko (2015)
10.1039/d0ta05796g
Highly efficient electrochemical energy conversion in a 3D hollow microenvironment: towards on-a-chip sensor applications
Letícia M. M. Ferro (2020)
10.1039/c6lc01586g
A fully disposable and integrated paper-based device for nucleic acid extraction, amplification and detection.
Ruihua Tang (2017)
10.1016/j.bios.2017.11.052
A multi-functional gold/iron-oxide nanoparticle-CNT hybrid nanomaterial as virus DNA sensing platform.
J. Lee (2018)
10.1016/j.bios.2020.112049
Nucleic acid amplification free biosensors for pathogen detection.
Y. Chen (2020)
10.1002/admi.201902048
Highly Symmetric and Extremely Compact Multiple Winding Microtubes by a Dry Rolling Mechanism
S. Moradi (2020)
10.1016/J.SNB.2017.04.080
Chemiresistive biosensors based on carbon nanotubes for label-free detection of DNA sequences derived from avian influenza virus H5N1
Y. Fu (2017)
10.1016/j.bios.2018.04.016
CIP2A immunosensor comprised of vertically-aligned carbon nanotube interdigitated electrodes towards point-of-care oral cancer screening.
Shaowei Ding (2018)
10.1002/smll.201703665
Assembly and Self-Assembly of Nanomembrane Materials-From 2D to 3D.
G. Huang (2018)
10.1002/9781119611103.ch23
Enzymatic Electrode–Electrolyte Interface Study During Electrochemical Sensing of Biomolecules
A. Kumar (2019)
10.1109/MARSS.2019.8860943
Optoacoustic detection of 3D microstructures in deep tissue-mimicking phantoms
Azaam Aziz (2019)
10.1039/c8lc00371h
A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood.
W. Liu (2018)
10.1016/j.talo.2020.100007
Biosensors for the detection of respiratory viruses: A review
Brayan Viana Ribeiro (2020)
10.1016/j.snr.2020.100008
Biologically modified microelectrode sensors provide enhanced sensitivity for detection of nucleic acid sequences from Mycobacterium tuberculosis
E. Blair (2020)
10.1016/J.ENSM.2018.05.002
3D Ag/NiO-Fe2O3/Ag nanomembranes as carbon-free cathode materials for Li-O2 batteries
Xueyi Lu (2019)
10.1088/1361-6463/AA66EC
Challenges and trends in magnetic sensor integration with microfluidics for biomedical applications
S. Cardoso (2017)
10.1002/pssa.201900825
Whispering Gallery Mode Optical Microresonators: Structures and Sensing Applications
Lu Cai (2020)
10.1002/ELAN.201700654
Electro‐oxidized Monolayer CVD Graphene Film Transducer for Ultrasensitive Impedimetric DNA Biosensor
A. Benvidi (2018)
10.3390/biomimetics5020024
Biomimetic Nanomembranes: An Overview
Z. Jakšić (2020)
10.1016/J.SNB.2018.02.174
Scalable fabrication and application of nanoscale IDE-arrays as multi-electrode platform for label-free biosensing
Lotta E. Delle (2018)
10.1002/admt.201901138
Silicon‐Based Integrated Label‐Free Optofluidic Biosensors: Latest Advances and Roadmap
Jiawei Wang (2020)
10.3390/TECHNOLOGIES7020035
Flexible Sensors—From Materials to Applications
Julio C. Costa (2019)
10.1007/5346_2017_18
Biosensorial Application of Impedance Spectroscopy with Focus on DNA Detection
M. Riedel (2017)
10.1039/C9TA03302E
Artificial electrode interfaces enable stable operation of freestanding anodes for high-performance flexible lithium ion batteries
Lixiang Liu (2019)
10.3390/CHEMOSENSORS6040043
Microfluidic Devices for Label-Free DNA Detection
Gorachand Dutta (2018)
10.1016/J.TRAC.2017.07.013
Point-of-care-testing of nucleic acids by microfluidics
Lu Zhang (2017)
10.1002/AELM.201800298
Rolled-up self-assembly of compact magnetic inductors, transformers and resonators
D. Karnaushenko (2018)
10.1002/adma.201902994
3D Self-Assembled Microelectronic Devices: Concepts, Materials, Applications.
D. Karnaushenko (2019)
10.1007/s12274-017-1720-2
Ultrasensitive detection of Ebola matrix protein in a memristor mode
B. Ibarlucea (2017)
10.1002/jmv.25422
A fluorescence method for homogeneous detection of influenza A DNA sequence based on guanine‐quadruplex‐N‐methylmesoporphyrin IX complex and assistance‐DNA inhibition
Y. Li (2019)
10.1002/adbi.201700193
Microsystems for Single‐Cell Analysis
Sonja M. Weiz (2018)
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