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Protective Polymer Coatings For High-Throughput, High-Purity Cellular Isolation
G. Romero, Jacob J. Lilly, N. S. Abraham, H. Shin, V. Balasubramaniam, Tadahide Izumi, Brad J Berron
Published 2015 · Materials Science, Medicine
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Cell-based therapies are emerging as the next frontier of medicine, offering a plausible path forward in the treatment of many devastating diseases. Critically, current methods for antigen positive cell sorting lack a high throughput method for delivering ultrahigh purity populations, prohibiting the application of some cell-based therapies to widespread diseases. Here we show the first use of targeted, protective polymer coatings on cells for the high speed enrichment of cells. Individual, antigen-positive cells are coated with a biocompatible hydrogel which protects the cells from a surfactant solution, while uncoated cells are immediately lysed. After lysis, the polymer coating is removed through orthogonal photochemistry, and the isolate has >50% yield of viable cells and these cells proliferate at rates comparable to control cells. Minority cell populations are enriched from erythrocyte-depleted blood to >99% purity, whereas the entire batch process requires 1 h and <$2000 in equipment. Batch scale-up is only contingent on irradiation area for the coating photopolymerization, as surfactant-based lysis can be easily achieved on any scale.
This paper references
Fluorescent polymeric nanocomposite films generated by surface-mediated photoinitiation of polymerization
H. Avens (2011)
Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
April M Kloxin (2009)
Quantification of non‐specific binding of magnetic micro‐ and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection
J. Chalmers (2010)
Systematic study of fluorescein-functionalized macrophotoinitiators for colorimetric bioassays.
J. Lee (2012)
Histone H2AX in DNA damage and repair.
O. Sedelnikova (2003)
Circulating tumor cells: approaches to isolation and characterization
M. Yu (2011)
Bioseparations Science and Engineering
R. Harrison (2002)
便秘 INTERFACE 精神科編
秀樹 石津 (2017)
Sensitive Immunofluorescent Staining of Cells via Generation of Fluorescent Nanoscale Polymer Films in Response to Biorecognition
H. Avens (2011)
Teratomas from pluripotent stem cells: A clinical hurdle
Chui-Yee Fong (2010)
Harnessing the potential of induced pluripotent stem cells for regenerative medicine
S. Wu (2011)
Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing beta-cell function
C C Lin (2011)
Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
S. Stott (2010)
Cell–cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing β-cell function
Chien-Chi Lin (2011)
Antigen-responsive, microfluidic valves for single use diagnostics.
Brad J Berron (2012)
Quantitative evaluation of oligonucleotide surface concentrations using polymerization-based amplification
R. R. Hansen (2008)
ACS Applied Materials & Interfaces Letter DOI: 10.1021/acsami
Interfacial Polymerization for Colorimetric Labeling of Protein Expression in Cells
Jacob L. Lilly (2014)
Cell - cell communication mimicry with poly ( ethylene glycol ) hydrogels for enhancing betacell function
C. C. Lin (2011)
Characterization of molecular transport in ultrathin hydrogel coatings for cellular immunoprotection.
Jacob L. Lilly (2015)
DNA damage responses to oxidative stress.
A. Barzilai (2004)
Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
Basak E Uygun (2010)
DNA Double-stranded Breaks Induce Histone H2AX Phosphorylation on Serine 139*
E. Rogakou (1998)
Current techniques for single-cell lysis
R. B. Brown (2008)
Thermodynamics of Micelle Formation of Ionic Surfactants: A Critical Assessment for Sodium Dodecyl Sulfate, Cetyl Pyridinium Chloride and Dioctyl Sulfosuccinate (Na Salt) by Microcalorimetric, Conductometric, and Tensiometric Measurements
A. Chatterjee (2001)
Practical Issues in High‐Speed Cell Sorting
L. Arnold (2010)
Biopolymer system for cell recovery from microfluidic cell capture devices.
A. Shah (2012)
Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation.
M. Brittberg (1994)
Concise Review: Circulating Endothelial Progenitor Cells for Vascular Medicine
T. Asahara (2011)
Induced pluripotent stem cells: current progress and potential for regenerative medicine.
G. Amabile (2009)
Histone H 2 AX in DNA damage and repair
O. A. Sedelnikova (2003)
The efficiency of magnetic-activated cell sorting and fluorescence-activated cell sorting in the decontamination of testicular cell suspensions in cancer patients.
M. Geens (2007)
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Hydrogel Patches on Live Cells through Surface-Mediated Polymerization.
Pei-Jung Wu (2017)
Comparison of eosin and fluorescein conjugates for the photoinitiation of cell-compatible polymer coatings
Jacob L. Lilly (2018)
SURFACE FUNCTIONALIZATION VIA PHOTOINITIATED RADICAL POLYMERIZATION FOR RARE CELL ISOLATION AND MECHANICAL PROTECTION
Calvin F. Cahall (2018)
The Role of Surface Receptor Density in Surface-Initiated Polymerizations for Cancer Cell Isolation.
Jacob L. Lilly (2016)
The heavy-atom effect on xanthene dyes for photopolymerization by visible light
Ji-eun Yoon (2019)
Coatings on mammalian cells: interfacing cells with their environment
Kara A. Davis (2019)
Genetically Encoded Stimuli-Responsive Cytoprotective Hydrogel Capsules for Single Cells Provide Novel Genotype–Phenotype Linkage
Rosario Vanella (2019)
Gelatin Based Polymer Cell Coating Improves Bone Marrow-Derived Cell Retention in the Heart after Myocardial Infarction
Anuhya Gottipati (2018)
Low-cost plug and play photochemistry reactor
David Dellal (2018)
SURFACE-INITIATED POLYMERIZATIONS FOR THE RAPID SORTING OF RARE CANCER CELLS
Jacob L. Lilly (2016)
Enzyme- and affinity biomolecule-mediated polymerization systems for biological signal amplification and cell screening.
Klara H. Malinowska (2016)
Aerobic Radical Polymerization Mediated by Microbial Metabolism
Gang Fan (2020)
Increased Yield of Gelatin Coated Therapeutic Cells Through Cholesterol Insertion.
Kara Davis (2020)
Polymeric Biomaterials: Diverse Functions Enabled by Advances in Macromolecular Chemistry.
Yingkai Liang (2017)
Cell Death Persists in Rapid Extrusion of Lysis-Resistant Coated Cardiac Myoblasts.
Calvin F. Cahall (2020)
Single-Cell Nanoencapsulation: From Passive to Active Shells.
Wongu Youn (2020)