Online citations, reference lists, and bibliographies.
← Back to Search

Real-Time Imaging Of Endocytosis And Intracellular Trafficking Of Semiconducting Polymer Dots.

Y. Han, X. Li, Haobin Chen, Xingjie Hu, Yao Luo, Ting Wang, Zejun Wang, Q. Li, Chunhai Fan, Jiye Shi, Lihua Wang, Y. Zhao, C. Wu, N. Chen
Published 2017 · Materials Science, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Semiconducting polymer dots (Pdots) have shown great promise in biomedical applications, including biosensing, drug delivery, and live imaging of cells and biomolecules. Insight into the mechanism and regulation of cellular uptake and intracellular metabolism of Pdots is important for the development of superior Pdots-based theranostic nanoconjugates. Herein, we performed real-time imaging of endocytosis and intracellular trafficking of a type of fluorescent Pdots that showed excellent biocompatibility in various types of cells. The endocytic routes and kinetics of Pdots were differently regulated in distinct cell types. Following endocytosis, Pdots were transported in vesicles along microtubule and destined for lysosomes. Furthermore, our results revealed exosome-mediated extracellular release of Pdots and have tracked the dynamic process at the single particle level. These results provide new insight into the design of more effective and selective imaging probes as well as drug carriers.
This paper references
10.1039/c1cc10991j
Conjugated polymer nanoparticles for small interfering RNA delivery.
J. H. Moon (2011)
10.1021/am5012595
Uniform small graphene oxide as an efficient cellular nanocarrier for immunostimulatory CpG oligonucleotides.
Jinli Sun (2014)
10.1038/nnano.2011.141
Gold nanoparticles for high-throughput genotyping of long-range haplotypes.
P. Chen (2011)
10.1111/febs.12110
Tetraspanin protein CD9 interacts with metalloprotease CD10 and enhances its release via exosomes
D. Mazurov (2013)
10.1038/531026a
The nanolight revolution is coming
X. Lim (2016)
10.1021/acsami.6b00811
Super Resolution Imaging of Nanoparticles Cellular Uptake and Trafficking.
D. van der Zwaag (2016)
10.1371/journal.pone.0024438
Effects of Transport Inhibitors on the Cellular Uptake of Carboxylated Polystyrene Nanoparticles in Different Cell Lines
T. dos Santos (2011)
10.1021/ar400324n
Physical and biochemical insights on DNA structures in artificial and living systems.
N. Chen (2014)
10.1038/nmat3819
A Broad Nanoparticle-Based Strategy for Tumor Imaging by Nonlinear Amplification of Microenvironment Signals
Y. Wang (2014)
10.1002/anie.201007461
Design of highly emissive polymer dot bioconjugates for in vivo tumor targeting.
C. Wu (2011)
10.1021/am508266p
Bifunctional magnetic-fluorescent nanoparticles: synthesis, characterization, and cell imaging.
Yanjiao Lu (2015)
10.1021/ja107196s
Bioconjugation of ultrabright semiconducting polymer dots for specific cellular targeting.
C. Wu (2010)
10.1021/acsnano.6b02386
In Vivo Dynamic Monitoring of Small Molecules with Implantable Polymer-Dot Transducer.
K. Sun (2016)
10.1038/icb.2011.20
Macropinocytosis: an endocytic pathway for internalising large gulps
J. P. Lim (2011)
10.1007/s11426-016-5596-x
Hetero-assembly of gold nanoparticles on a DNA origami template
J. Chao (2016)
10.1002/anie.201202356
Reconfigurable three-dimensional DNA nanostructures for the construction of intracellular logic sensors.
H. Pei (2012)
10.1126/SCIENCE.1104274
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
X. Michalet (2005)
10.1074/JBC.M407167200
Macropinocytosis Is the Endocytic Pathway That Mediates Macrophage Foam Cell Formation with Native Low Density Lipoprotein*
H. Kruth (2005)
10.1021/JA074936K
Imaging and tracking of tat peptide-conjugated quantum dots in living cells: new insights into nanoparticle uptake, intracellular transport, and vesicle shedding.
G. Ruan (2007)
Impair Embryonic Development in Zebrafish
E. Granger (2014)
10.1021/am100435k
Conjugated polymer nanoparticles for drug delivery and imaging.
Xuli Feng (2010)
10.1038/nmat3149
Tuning upconversion through energy migration in core-shell nanoparticles.
Feng Wang (2011)
10.1038/nature12354
Ultra-sensitive fluorescent proteins for imaging neuronal activity
Tsai-Wen Chen (2013)
10.1039/B105159H
Aggregation-induced emission of 1-methyl-1,2,3,4,5-pentaphenylsilole.
J. Luo (2001)
10.1021/nn101277w
Endo- and exocytosis of zwitterionic quantum dot nanoparticles by live HeLa cells.
X. Jiang (2010)
10.1002/adma.201400451
A bubble-mediated intelligent microscale electrochemical device for single-step quantitative bioassays.
F. Yang (2014)
10.1021/am4033857
Trafficking of gold nanorods in breast cancer cells: uptake, lysosome maturation, and elimination.
W. Zhang (2013)
10.1021/am506577r
Near-infrared fluorescent semiconducting polymer dots with high brightness and pronounced effect of positioning alkyl chains on the comonomers.
Chuan-Pin Chen (2014)
10.1038/NMAT1390
Quantum dot bioconjugates for imaging, labelling and sensing
Igor L. Medintz (2005)
10.1039/c3cs60309a
Nanoscale optical probes for cellular imaging.
H. Xu (2014)
10.1007/s11426-016-0020-9
Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope
S. Wang (2016)
10.1073/pnas.1605841113
Theranostic near-infrared fluorescent nanoplatform for imaging and systemic siRNA delivery to metastatic anaplastic thyroid cancer
Y. Liu (2016)
10.1021/ar400023s
Functional surface engineering of C-dots for fluorescent biosensing and in vivo bioimaging.
Changqin Ding (2014)
10.7150/thno.16127
Silica Nanoparticles Target a Wnt Signal Transducer for Degradation and Impair Embryonic Development in Zebrafish
Hongyang Yi (2016)
10.1021/nn800590n
Multicolor conjugated polymer dots for biological fluorescence imaging.
C. Wu (2008)
10.1002/adma.201604850
Small Photoblinking Semiconductor Polymer Dots for Fluorescence Nanoscopy.
X. Chen (2017)
10.1016/j.ccr.2013.01.008
Role of macrophage targeting in the antitumor activity of trabectedin.
G. Germano (2013)
10.1002/anie.201410895
Gold-nanoparticle-mediated jigsaw-puzzle-like assembly of supersized plasmonic DNA origami.
Guangbao Yao (2015)
Uptake, Lysosome Maturation, and Elimination
D. Mazurov (2013)
Plasmonic DNA Origami
J. Sun (2015)
10.1021/acsami.5b01786
Dual-responsive polymer coated superparamagnetic nanoparticle for targeted drug delivery and hyperthermia treatment.
S. Patra (2015)
10.1016/j.biomaterials.2011.10.070
The cytotoxicity of cadmium-based quantum dots.
N. Chen (2012)
10.1126/science.1127344
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
E. Betzig (2006)
10.1002/CHIN.201105276
Functional Nanoprobes for Ultrasensitive Detection of Biomolecules
Shiping Song (2011)
10.1021/nn204448x
High-speed imaging of Rab family small GTPases reveals rare events in nanoparticle trafficking in living cells.
Peter Sandin (2012)
10.1002/CHIN.201327238
Highly Fluorescent Semiconducting Polymer Dots for Biology and Medicine.
C. Wu (2013)
10.1021/bm1007103
Mechanism of cellular uptake of highly fluorescent conjugated polymer nanoparticles.
Lawrence P. Fernando (2010)
10.1002/anie.201403236
Single-particle tracking and modulation of cell entry pathways of a tetrahedral DNA nanostructure in live cells.
L. Liang (2014)
10.1021/nn401800u
Particles on the move: intracellular trafficking and asymmetric mitotic partitioning of nanoporous polymer particles.
Yan Yan (2013)
10.1021/am502733n
Size-dependent property and cell labeling of semiconducting polymer dots.
K. Sun (2014)
10.1016/j.semcdb.2014.04.011
The role of the cytoskeleton and molecular motors in endosomal dynamics
Elizabeth Granger (2014)
10.1088/0957-4484/20/42/425103
The biocompatibility of fluorescent nanodiamonds and their mechanism of cellular uptake.
V. Vaijayanthimala (2009)
Cell Formation with Native Low Density Lipoprotein
C. Wu (2005)
10.1038/nrm2728
Rab GTPases as coordinators of vesicle traffic
H. Stenmark (2009)
10.1021/acsami.5b04766
Highly Enhanced Fluorescence of CdSeTe Quantum Dots Coated with Polyanilines via In-Situ Polymerization and Cell Imaging Application.
J. Xue (2015)
10.1002/adhm.201300294
Autophagy-sensitized cytotoxicity of quantum dots in PC12 cells.
X. Li (2014)
10.1016/j.molcel.2015.02.033
Imaging live-cell dynamics and structure at the single-molecule level.
Z. Liu (2015)
10.1021/nn301308w
Importance of having low-density functional groups for generating high-performance semiconducting polymer dots.
Xuanjun Zhang (2012)



This paper is referenced by
10.1021/ACSANM.7B00078
Poly(thymine)-Templated Selective Formation of Copper Nanoparticles for Alkaline Phosphatase Analysis Aided by Alkyne–Azide Cycloaddition “Click” Reaction
D. Yang (2017)
10.1039/C8RA06665E
Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
Lijuan Chen (2018)
10.1039/C9NJ03277K
Conjugated polymer dots for biocompatible siRNA delivery
Fei Wang (2019)
10.2217/nnm-2020-0106
Trojan-horse monocyte-mediated delivery of conjugated polymer nanoparticles for improved photodynamic therapy of glioblastoma.
Luis E Ibarra (2020)
10.1039/C9QI00424F
Click reactions and intramolecular condensation reactions on azido-adamantyl-functionalized tin sulfide clusters
Annikka Engel (2019)
10.2217/nnm-2017-0292
Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells.
Luis E Ibarra (2018)
10.1039/c8sc00367j
Guiding protein delivery into live cells using DNA-programmed membrane fusion† †Electronic supplementary information (ESI) available: Experimental sections, supporting tables and figures. See DOI: 10.1039/c8sc00367j
L. Sun (2018)
10.1039/C9MH00388F
Reprogramming of cancer invasiveness and macrophage education via a nanostructured antagonist of the TGFβ receptor
Chang Liu (2019)
10.1111/cpr.12556
Live‐cell imaging of octaarginine‐modified polymer dots via single particle tracking
Y. Luo (2019)
10.1021/acs.nanolett.9b01320
An Intelligent DNA Nanorobot with in Vitro Enhanced Protein Lysosomal Degradation of HER2.
Wenjuan Ma (2019)
10.1039/d0bm01451f
NIR/photoacoustic imaging of multitype gallbladder cancer using carboxyl/amino functionalized polymer dots.
Yidian Yang (2020)
10.1039/c8sc03510e
Molecular design of near-infrared fluorescent Pdots for tumor targeting: aggregation-induced emission versus anti-aggregation-caused quenching† †Electronic supplementary information (ESI) available: Experimental section, NMR spectra, and additional information as noted in the text. See DOI: 10.1039/
Wei-Kai Tsai (2019)
Semantic Scholar Logo Some data provided by SemanticScholar