← Back to Search
Versatile Conjugated Polymer Nanoparticles For High-Resolution O2 Imaging In Cells And 3D Tissue Models.
Ruslan I. Dmitriev, Sergey M. Borisov, Heiko Duessmann, Shiwen Sun, B. Mueller, J. Prehn, Vladimir P. Baklaushev, I. Klimant, D. Papkovsky
Published 2015 · Materials Science, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
High brightness, chemical and photostability, tunable characteristics, and spectral and surface properties are important attributes for nanoparticle probes designed for live cell imaging. We describe a class of nanoparticles for high-resolution imaging of O2 that consists of a substituted conjugated polymer (polyfluorene or poly(fluorene-alt-benzothiadiazole)) acting as a FRET antenna and a fluorescent reference with covalently bound phosphorescent metalloporphyrin (PtTFPP, PtTPTBPF). The nanoparticles prepared from such copolymers by precipitation method display stability, enhanced (>5-10 times) brightness under one- and two-photon excitation, compatibility with ratiometric and lifetime-based imaging modes, and low toxicity for cells. Their cell-staining properties can be modulated with positively and negatively charged groups grafted to the backbone. The "zwitter-ionic" nanoparticles show high cell-staining efficiency, while their cell entry mechanisms differ for the different 3D models. When injected in the bloodstream, the cationic and anionic nanoparticles show similar distribution in vivo. These features and tunable properties make the conjugated polymer based phosphorescent nanoparticles a versatile tool for quantitative O2 imaging with a broad range of cell and 3D tissue models.
This paper references
Referenced luminescent sensing and imaging with digital color cameras: A comparative study
R. Meier (2013)
Fluorescence lifetime imaging – techniques and applications
W. Becker (2012)
Indicators for optical oxygen sensors
M. Quaranta (2012)
Donor-acceptor-donor fluorene derivatives for two-photon fluorescence lysosomal imaging.
S. Yao (2010)
Two-photon nano-PEBBLE sensors: subcellular pH measurements.
A. Ray (2011)
Effects of particle size and surface charge on cellular uptake and biodistribution of polymeric nanoparticles.
Chunbai He (2010)
Understanding biophysicochemical interactions at the nano-bio interface.
A. Nel (2009)
Kinetic analysis of local oxygenation and respiratory responses of mammalian cells using intracellular oxygen-sensitive probes and time-resolved fluorometry.
A. Zhdanov (2014)
A ratiometric luminescent oxygen sensor based on a chemically functionalized quantum dot.
M. Amelia (2011)
Photophysics and photochemistry of oxygen sensors based on luminescent transition-metal complexes
E. Carraway (1991)
Multi-parametric O₂ imaging in three-dimensional neural cell models with the phosphorescent probes.
R. Dmitriev (2015)
Design of Metalloporphyrin-Based Dendritic Nanoprobes for Two-Photon Microscopy of Oxygen.
A. Y. Lebedev (2008)
In vivo imaging of brain metabolism activity using a phosphorescent oxygen-sensitive probe
V. Tsytsarev (2013)
Reaction-based two-photon probes for mercury ions: fluorescence imaging with dual optical windows.
A. S. Rao (2012)
Fluorescent nanoparticles for intracellular sensing: a review.
M. J. Ruedas-Rama (2012)
Two-Photon Antenna-Core Oxygen Probe with Enhanced Performance
Emmanuel Roussakis (2014)
Click-assembled, oxygen-sensing nanoconjugates for depth-resolved, near-infrared imaging in a 3D cancer model.
A. J. Nichols (2014)
Ratiometric single-nanoparticle oxygen sensors for biological imaging.
C. Wu (2009)
Targeted nanoparticles in imaging: paving the way for personalized medicine in the battle against cancer.
S. J. Shin (2013)
The Unique Role of Nanoparticles in Nanomedicine: Imaging, Drug Delivery and Therapy
Tennyson L. Doane (2012)
New NIR-emitting complexes of platinum(II) and palladium(II) with fluorinated benzoporphyrins
S. Borisov (2009)
Synthesis of Phosphorescent Asymmetrically π-Extended Porphyrins for Two-Photon Applications
T. Esipova (2014)
Ratiometric molecular sensor for monitoring oxygen levels in living cells.
T. Yoshihara (2012)
Targetable phosphorescent oxygen nanosensors for the assessment of tumor mitochondrial dysfunction by monitoring the respiratory activity.
X. Wang (2014)
Fiber‐optic oxygen microsensors, a new tool in aquatic biology
I. Klimant (1995)
Imaging oxygen in neural cell and tissue models by means of anionic cell-permeable phosphorescent nanoparticles
Ruslan I. Dmitriev (2014)
Frontiers in Optical Imaging of Cerebral Blood Flow and Metabolism
A. Devor (2012)
π-Conjugated Polymers for Organic Electronics and Photovoltaic Cell Applications†
A. Facchetti (2011)
O. S. Wolfbeis (2008)
The third dimension bridges the gap between cell culture and live tissue
F. Pampaloni (2007)
Oxygen microscopy by two-photon-excited phosphorescence.
Olga S. Finikova (2008)
Optical methods for sensing and imaging oxygen: materials, spectroscopies and applications.
Xu-Dong Wang (2014)
Intracellular probes for imaging oxygen concentration: how good are they?
R. Dmitriev (2015)
Comparative bioenergetic assessment of transformed cells using a cell energy budget platform.
A. Zhdanov (2011)
Planar optrodes: a new tool for fine scale measurements of two-dimensional O2 distribution in benthic communities
R. N. Glud (1996)
Precipitation as a simple and versatile method for preparation of optical nanochemosensors.
S. Borisov (2009)
Absorption and emission spectroscopic characterization of platinum-octaethyl-porphyrin (PtOEP)
A. Bansal (2006)
Oxygen dynamics in the rhizosphere of Zostera marina: A two‐dimensional planar optode study
M. Frederiksen (2006)
A dual-emissive-materials design concept enables tumour hypoxia imaging.
G. Zhang (2009)
Polymer-encapsulated organic nanoparticles for fluorescence and photoacoustic imaging.
Kai Li (2014)
Self-referenced RGB colour imaging of intracellular oxygen
Xu-Dong Wang (2011)
Rational Design of High Performance Conjugated Polymers for Organic Solar Cells
Hua-xing Zhou (2012)
pH-sensitive perylene bisimide probes for live cell fluorescence lifetime imaging.
D. Aigner (2014)
Optical nanosensors--an enabling technology for intracellular measurements.
J. Aylott (2003)
Water-soluble conjugated polymers for imaging, diagnosis, and therapy.
Chunlei Zhu (2012)
Conjugated Polymer‐Based Chemical Sensors
D. Mcquade (2000)
Click - Assembled , OxygenSensing Nanoconjugates for DepthResolved , Near - Infrared Imaging in a 3 D Cancer Model
A. J. Nichols (2014)
Conjugated polymer dots for multiphoton fluorescence imaging.
C. Wu (2007)
Multiphoton absorbing materials: molecular designs, characterizations, and applications.
G. He (2008)
Synthesis of light-emitting conjugated polymers for applications in electroluminescent devices.
A. Grimsdale (2009)
Correlation between intraluminal oxygen gradient and radial partitioning of intestinal microbiota.
L. Albenberg (2014)
Two-photon absorption and the design of two-photon dyes.
M. Pawlicki (2009)
Pharmacological inhibition of endocytic pathways: is it specific enough to be useful?
A. Ivanov (2008)
Measurement of photoluminescence quantum yields. Review
G. Crosby (1971)
A Phosphorescent Nanoparticle‐Based Probe for Sensing and Imaging of (Intra)Cellular Oxygen in Multiple Detection Modalities
A. Kondrashina (2012)
Correction to “Electron-Exchange Assisted Photon Energy Up-Conversion in Thin Films of π-Conjugated Polymeric Composites”
P. Keivanidis (2011)
Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment
P. Provenzano (2008)
Small molecule phosphorescent probes for O2 imaging in 3D tissue models.
R. Dmitriev (2014)
Biological detection by optical oxygen sensing.
D. Papkovsky (2013)
Nanoparticle PEBBLE sensors in live cells and in vivo.
Y. K. Lee (2009)
Direct measurement of local oxygen concentration in the bone marrow of live animals
J. Spencer (2014)
A MODULAR LUMINESCENCE LIFETIME IMAGING SYSTEM FOR MAPPING OXYGEN DISTRIBUTION IN BIOLOGICAL SAMPLES
G. Holst (1998)
Endocytosis at the nanoscale.
I. Cantón (2012)
Intracellular O2 sensing probe based on cell-penetrating phosphorescent nanoparticles.
A. Fercher (2011)
Optical nanosensors--smart tools in bioanalytics.
S. Borisov (2008)
Ratiometric optical oxygen sensing: a review in respect of material design.
Y. Feng (2012)
Ultrasmall Phosphorescent Polymer Dots for Ratiometric Oxygen Sensing and Photodynamic Cancer Therapy
H. Shi (2014)
Tunable fluorescent/phosphorescent platinum(II) porphyrin-fluorene copolymers for ratiometric dual emissive oxygen sensing.
H. Xiang (2012)
Imaging of neurosphere oxygenation with phosphorescent probes.
R. Dmitriev (2013)
This paper is referenced by
Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes
D. Papkovsky (2015)
Recent advances in fluorescent probes for cellular antioxidants: Detection of NADH, hNQO1, H2S, and other redox biomolecules
S. Park (2021)
Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity
Hannu Välimäki (2020)
Nanoparticle-based fluoroionophore for analysis of potassium ion dynamics in 3D tissue models and in vivo.
B. Müller (2018)
Thiophene-derived polymer dots for imaging endocytic compartments in live cells and broad-spectrum bacterial killing
Kenath Priyanka Prasad (2017)
Facile synthesis of a ratiometric oxygen nanosensor for cellular imaging.
Sisi Lu (2016)
Rapid Polymer Conjugation Strategies for the Generation of pH-Responsive, Cancer Targeting, Polymeric Nanoparticles.
Weishuo Li (2018)
Intestinal Stem Cells: Methods and Protocols
Paloma Ordóñez-Morán (2020)
Use of Fluorescence Lifetime Imaging Microscopy (FLIM) as a Timer of Cell Cycle S Phase
I. A. Okkelman (2016)
Multi-parametric imaging of tumor spheroids with ultra-bright and tunable nanoparticle O2 probes
R. Dmitriev (2015)
Exploring Π-conjugated carbon nanomaterials for biomedical applications
Kenath Priyanka Prasad (2016)
Conjugated Polymer Nanoparticles toward In Vivo Theranostics – Focus on Targeting, Imaging, Therapy, and the Importance of Clearance
A. Kuehne (2017)
Simultaneous imaging of intracellular pH and O2 using functionalized semiconducting polymer dots.
W. Xu (2016)
Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
H. Wang (2017)
Phosphorescent ion-paired iridium(III) complex for ratiometric and time-resolved luminescence imaging of intracellular biothiols.
S. Liu (2016)
Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications
Andreas Steinegger (2020)
Multiparametric Optical Bioimaging Reveals the Fate of Epoxy Crosslinked Biomeshes in the Mouse Subcutaneous Implantation Model
V. Elagin (2020)
Amphiphilic Fluorine-Containing Block Copolymers as Carriers for Hydrophobic PtTFPP for Dissolved Oxygen Sensing, Cell Respiration Monitoring and In Vivo Hypoxia Imaging with High Quantum Efficiency and Long Lifetime
Jiaze Li (2018)
Time-resolved FRET in AgInS2/ZnS-CdSe/ZnS quantum dot systems.
Roman V Evstigneev (2019)
Phosphorescence Lifetime Imaging (PLIM): State of the Art and Perspectives
Pavel S. Chelushkin (2019)
Multi-Parametric Imaging of Hypoxia and Cell Cycle in Intestinal Organoid Culture.
I. A. Okkelman (2017)
Conjugated Polymers for Two‐Photon Live Cell Imaging
Shuang Li (2018)
Quick and simple integration of optical oxygen sensors into glass-based microfluidic devices
L. C. Lasave (2015)
Amplified singlet oxygen generation in metallated-porphyrin doped conjugated polymer nanoparticles
Ramiro M. Spada (2018)
Highly Luminescent Ln-MOFs Based on 1,3-Adamantanediacetic Acid as Bifunctional Sensor
K. Zheng (2018)
Phosphorescent Polymeric Thermometers for In Vitro and In Vivo Temperature Sensing with Minimized Background Interference
Zejing Chen (2016)
Generation of Fluorescent and Stable Conjugated Polymer Nanoparticles with Hydrophobically Modified Poly(acrylate)s
L. Yan (2016)
Ratiometric optical nanoprobes enable accurate molecular detection and imaging.
X. Huang (2018)
Conjugated Polymer Nanoparticles for Fluorescence Imaging and Sensing of Neurotransmitter Dopamine in Living Cells and the Brains of Zebrafish Larvae.
Chenggen Qian (2015)
Versatile Polymer Nanoparticles as Two‐Photon‐Triggered Photosensitizers for Simultaneous Cellular, Deep‐Tissue Imaging, and Photodynamic Therapy
Liang Guo (2017)
Luminescent Conjugated Polymer Dots for Biomedical Applications
G. Li (2021)
Rational design of semiconducting polymer brushes as cancer theranostics
Zhen Yang (2020)See more