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Inhibition Of Human Cell Apoptosis By Silkworm Hemolymph

S. Choi, W. J. Rhee, T. Park
Published 2002 · Biology, Medicine

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Many studies on preventing apoptosis have been carried out from the viewpoint of anti‐apoptotic cloned‐gene expressions inside cells, whereas in this study, we investigated the inhibition of apoptosis by the addition of silkworm hemolymph, a natural compound, from outside of the cells. In a previous study, we reported the inhibition effect of silkworm hemolymph on the baculovirus‐induced insect cell apoptosis. Using the vaccinia virus‐HeLa cell system as a model system in this study, we found that silkworm hemolymph, the insect serum, inhibits apoptosis not only in the insect cell system but also in the human cell system. The vaccinia virus‐induced HeLa cell apoptosis was analyzed using DNA electrophoresis, TUNEL, and flow cytometry, and the resulting data confirmed that silkworm hemolymph inhibits human cell apoptosis. The inhibition of apoptosis due to silkworm hemolymph was not caused by an inhibition of virus binding and internalization steps, nor did silkworm hemolymph interfere with the virus production. The inhibition of apoptosis by silkworm hemolymph decreased the cell detachment from an adhering surface. With these characteristics, silkworm hemolymph can be effectively used to minimize cell death in commercial animal cell culture.
This paper references
10.1006/BBRC.2001.5148
Isolation and characterization of an apoptosis-inhibiting component from the hemolymph of Bombyx mori.
E. Kim (2001)
10.1007/978-3-642-75608-5_2
Poxvirus expression vectors.
B. Moss (1992)
10.1159/000150024
Differences in the susceptibility of human blood cell lines to vaccinia virus.
B. Pogo (1988)
10.1023/A:1018484309194
Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph
S. Ha (2004)
10.1099/0022-1317-67-10-2067
Vaccinia virus expression vectors.
M. Mackett (1986)
African Swine Fever Virus Gene A 179 L , a Viral Homologue of bcl - 2 , Protects Cells from Programmed Cell Death
A. Brun (1996)
10.1007/BF02931888
Quantitative measurement of general odorant using electroantennogram of male silkworm moth,Bombyx mori
Eung Sik Yun (2000)
10.1038/348334A0
Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death
D. Hockenbery (1990)
10.1016/0092-8674(91)90007-L
Induction of bcl-2 expression by epstein-barr virus latent membrane protein 1 protects infected B cells from programmed cell death
S. Henderson (1991)
Cold Spring Harbor Laboratory: Cold Spring Harbor
L D Tomei (1991)
10.1128/MCB.5.12.3403
Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.
S. Chakrabarti (1985)
10.1006/VIRO.1998.9077
Inhibition of ICE-like proteases inhibits apoptosis and increases virus production during adenovirus infection.
S. Chiou (1998)
10.1021/bp990093s
Kinetic Effect of Silkworm Hemolymph on the Delayed Host Cell Death in an Insect Cell‐Baculovirus System
Won Jong Rhee (1999)
10.1038/311067A0
Live recombinant vaccinia virus protects chimpanzees against hepatitis B
B. Moss (1984)
10.1006/VIRO.1997.8985
Apoptosis induced by a postbinding step of vaccinia virus entry into Chinese hamster ovary cells.
A. Ramsey-Ewing (1998)
10.1111/j.1365-3083.1991.tb01585.x
Highly Efficient Expression of Proteins Encoded by Recombinant Vaccinia Virus in Lymphocytes
J. Alonso (1991)
10.1128/JVI.69.1.517-522.1995
Isolation of a monoclonal antibody which blocks vaccinia virus infection.
W. Chang (1995)
Apoptosis: The Molecular Basis of Cell Death
L. D. Tomei (1991)
Flow cytometric analysis of the effect of silkworm hemolymph on the baculovirus-induced insect cell apoptosis
Rhee Won Jong (2001)
10.1038/352345A0
Wild-type p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interleukin-6
E. Yonish-Rouach (1991)
10.1007/BF00174223
Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture
S. Ha (1996)
10.1006/BBRC.2000.2592
Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.
W. J. Rhee (2000)
10.1006/VIRO.1998.9395
Rubella virus induces apoptosis in culture cells.
K. Pugachev (1998)
10.1007/BF02931887
High-efficient expression of porcine IL-2 with recombinant baculovirus infected silkworm,Bombyx mori
S. Inumaru (2000)



This paper is referenced by
10.1007/S11814-010-0278-4
Cellular engineering for the high-level production of recombinant proteins in mammalian cell systems
J. Park (2010)
10.1109/ICBBE.2011.5781430
Notice of RetractionApoptosis Induced by Actinomycin D in Spodoptera litura Cells and the Effect of Two Insect Hemolymph on Apoptosis
R. Zhang (2011)
The Mulberry Silkworm — A New Source of Bioactive Proteins
Wojska Polskiego (2016)
Silkworm (Bombyx mori) and its constituents: A fascinating insect in science and research
M. Soumya (2017)
10.15429/JKOMOR.2018.18.2.83
Optimization of Mixing Ratio of Mulberry Leaf, Mulberry Fruit, and Silkworm for Amelioration of Metabolic Syndrome
M. Kim (2018)
10.4163/JNH.2018.51.3.201
고지방식이 비만 유도 mouse에서 누에체액의 항비만 및 LDL-cholesterol 저하효과
You Ree Nam (2018)
10.1016/J.PROCBIO.2012.02.001
Expression of anti-apoptotic 30Kc6 gene inhibiting hyperosmotic pressure-induced apoptosis in antibody-producing Chinese hamster ovary cells
Zesong Wang (2012)
10.1002/BIT.20550
Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production.
S. Choi (2005)
10.1002/biot.201400253
Dimerization of 30Kc19 protein in the presence of amphiphilic moiety and importance of Cys-57 during cell penetration
Hee Ho Park (2014)
10.1007/s00253-016-7689-z
α-Galactosidase delivery using 30Kc19-human serum albumin nanoparticles for effective treatment of Fabry disease
Hong Jai Lee (2016)
10.1016/J.PROCBIO.2014.05.008
Identification and characterization of a novel cell-penetrating peptide of 30Kc19 protein derived from Bombyx mori
Hee Ho Park (2014)
10.1021/bp034130y
Inhibition of Apoptosis by a Bombyx mori Gene
E. J. Kim (2004)
10.1590/S1516-89132005000400011
Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph
T. Raffoul (2005)
PHOTOPERIOD INDUCED INSTAR-SPECIFIC CLOCK-SHIFTING IN THE CIRCADIAN PROTEIN AND AMINO ACID RHYTHMS IN THE LARVAL HAEMOLYMPH OF THE SILKWORM, BOMBYX MORI
B. Sailaja (2011)
10.1016/S0006-291X(03)01425-6
Inhibition of apoptosis by recombinant 30K protein originating from silkworm hemolymph.
E. J. Kim (2003)
10.11606/D.87.2011.TDE-10022012-120107
ESTUDO DO EFEITO DE UMA PROTEÍNA ANTIAPOPTÓTICA OBTIDA DA HEMOLINFA DE LONOMIA OBLIQUA SOBRE AS MITOCÔNDRIAS DE CÉLULAS SF-9.
L. M. Martins (2011)
10.1007/s00253-012-4203-0
Enhancement of recombinant human EPO production and glycosylation in serum-free suspension culture of CHO cells through expression and supplementation of 30Kc19
J. Park (2012)
10.1007/s12257-016-0588-4
Protective effects of silkworm hemolymph extract and its fractions on UV-induced photoaging
J. Park (2017)
10.1002/biot.201600040
Protein‐stabilizing and cell‐penetrating properties of α‐helix domain of 30Kc19 protein
Jina Ryu (2016)
10.1016/J.PROCBIO.2015.03.008
Enhancement of human erythropoietin production in Chinese hamster ovary cells through supplementation of 30Kc19-30Kc6 fusion protein
Hee Ho Park (2015)
10.3390/ijms12074456
Silkworm Hemolymph Down-Regulates the Expression of Endoplasmic Reticulum Chaperones under Radiation-Irradiation
K. Lee (2011)
10.1111/1744-7917.12119
Antiapoptotic activity of 30 kDa lipoproteins family from fat body tissue of silkworm, Bombyx mori
Britto Cathrin Pakkianathan (2015)
10.1007/BF02931854
Enhanced production of recombinant protein inEscherichia coli using silkworm hemolymph
J. Kim (2005)
10.4014/mbl.1901.01015
Silk Protein as a Fetal Bovine Serum Substitute for Animal Cell Culture
You-Young Jo (2019)
10.1002/BIT.21023
Enhancement of recombinant protein production in Chinese hamster ovary cells through anti‐apoptosis engineering using 30Kc6 gene
S. Choi (2006)
10.1002/9783527811410.ch11
Current Considerations and Future Advances in Chemically Defined Medium Development for the Production of Protein Therapeutics in CHO Cells
W. L. Ling (2019)
10.1371/journal.pone.0108761
Crystal Structure of Bombyx mori Lipoprotein 6: Comparative Structural Analysis of the 30-kDa Lipoprotein Family
A. Pietrzyk (2014)
10.1016/J.PROCBIO.2017.08.021
Enzyme delivery using protein-stabilizing and cell-penetrating 30Kc19α protein nanoparticles
Hee Ho Park (2017)
10.1021/bp0702065
Inhibition of HeLa Cell Apoptosis by Storage‐Protein 2
W. J. Rhee (2007)
SYNERGETIC EFFECT OF ASCORBIC ACID AND LEMON JUICE ON THE GROWTH AND PROTEIN SYNTHESIS IN THE SILKWORM , BOMBYX MORI AND ITS INFLUENCE ON ECONOMIC TRAITS OF SERICULTURE
N. Thulasi (2013)
10.4162/nrp.2011.5.3.214
High glucose diets shorten lifespan of Caenorhabditis elegans via ectopic apoptosis induction
S. Choi (2011)
DETERMINATION OF MINIMUM EFFECTIVE DOSE OF ZINC CHLORIDE FOR MODULATION OF METABOLISM AND SILK PRODUCTION IN THE SILKWORM, BOMBYX MORI
S. Kavitha (2011)
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