Online citations, reference lists, and bibliographies.
← Back to Search

Site-specific Immobilization And Micrometer And Nanometer Scale Photopatterning Of Yellow Fluorescent Protein On Glass Surfaces.

N. Reynolds, J. Tucker, Paul A. Davison, John A. Timney, C. Hunter, G. Leggett
Published 2009 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
A simple method is described for the site-specific attachment of yellow fluorescent protein (YFP) to glass surfaces on length scales ranging from tens of micrometers to ca. 200 nm. 3-Mercaptopropyl(triethoxy silane) is adsorbed onto a glass substrate and subsequently derivatized using a maleimide-functionalized oligomer of ethylene glycol. The resulting protein-resistant surface is patterned by exposure to UV light, causing photochemical degradation of the oligo(ethylene glycol) units to yield aldehyde groups in exposed regions. These are covalently bound to N-(5-amino-1-carboxypentyl)iminoacetic acid, yielding a nitrilotriacetic acid (NTA)-functionalized surface, which following complexation with Ni(2+), is coupled to His-tagged YFP. Using scanning near-field photolithography, in which a UV laser coupled to a scanning near-field optical microscope is utilized as the light source for photolithography, it is possible to fabricate lines of protein smaller than 200 nm, in which the biomolecules remain strongly optically active, facilitating the acquisition of diffraction-limited fluorescence images by confocal microscopy.
This paper references
Protein nanostructures formed via direct-write dip-pen nanolithography.
K. Lee (2003)
One-step photochemical introduction of nanopatterned protein-binding functionalities to oligo(ethylene glycol)-terminated self-assembled monolayers.
R. E. Ducker (2007)
Direct Patterning of Modified Oligonucleotides on Metals and Insulators by Dip-Pen Nanolithography
L. Demers (2002)
Molecular Recognition-Mediated Fabrication of Protein Nanostructures by Dip-Pen Lithography
J. Hyun (2002)
Protein Nanoarrays Generated By Dip-Pen Nanolithography
K. Lee (2002)
“Constructive Nanolithography”: Inert Monolayers as Patternable Templates for In‐Situ Nanofabrication of Metal–Semiconductor–Organic Surface Structures—A Generic Approach
R. Maoz (2000)
Fabrication of biomolecular nanostructures by scanning near-field photolithography of oligo(ethylene glycol)-terminated self-assembled monolayers.
Matthew Montague (2007)
Constructive Microlithography: Electrochemical Printing of Monolayer Template Patterns Extends Constructive Nanolithography to the Micrometer−Millimeter Dimension Range
S. Hoeppener (2003)
Fabrication of biological nanostructures by scanning near-field photolithography of chloromethylphenylsiloxane monolayers.
S. Sun (2006)
Directed formation of micro- and nanoscale patterns of functional light-harvesting LH2 complexes.
Nicholas P Reynolds (2007)
Bioactive protein nanoarrays on nickel oxide surfaces formed by dip-pen nanolithography.
J. Nam (2004)
"Dip-Pen" nanolithography
Piner (1999)
Positioning protein molecules on surfaces: A nanoengineering approach to supramolecular chemistry
G. Liu (2002)

This paper is referenced by
Nanofabrication of bioinspired architectures with light harvesting proteins
M. E. Marun (2009)
A Biotin Micropatterned Surface Generated by Photodestruction Serves as a Novel Platform for Microtubule Organisation and DNA Hybridisation
A. Biswas (2013)
DNA nanoarchitectonics: assembled DNA at interfaces.
S. Howorka (2013)
Surface modification of biomaterials by peptide functionalisation
L. Birchall (2011)
Micrometer and Nanometer Scale Photopatterning of Proteins on Glass Surfaces by Photo-degradation of Films Formed from Oligo(Ethylene Glycol) Terminated Silanes
G. Tizazu (2012)
The Effect of Image Potential on the Current-Voltage Characteristics of a Ferritin-layer
E. Bang (2010)
Controlled Modification of Protein-Repelling Self-Assembled Monolayers by Ultraviolet Light: The Effect of the Wavelength
Y. L. Jeyachandran (2012)
Painting with biomolecules at the nanoscale: biofunctionalization with tunable surface densities.
Robert Schlapak (2012)
Native laser lithography of His-tagged proteins by uncaging of multivalent chelators.
Maniraj Bhagawati (2010)
Oriented Protein Nanoarrays on Block Copolymer Template.
L. Shen (2016)
Controlled immobilization of His-tagged proteins for protein-ligand interaction experiments using Ni²⁺-NTA layer on glass surfaces.
C. Cherkouk (2015)
Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device
C. Johnson (2010)
Immobilization of chiral Rh catalyst on glass and application to asymmetric transfer hydrogenation of aryl ketones in aqueous media
Tanyu Cheng (2014)
From Monochrome to Technicolor: Simple Generic Approaches to Multicomponent Protein Nanopatterning Using Siloxanes with Photoremovable Protein-Resistant Protecting Groups
Osama el Zubir (2017)
Preparation of Biomolecule Microstructures and Microarrays by Thiol–ene Photoimmobilization
Dirk Weinrich (2010)
Maskless Ultraviolet Projection Lithography with a Biorepelling Monomolecular Resist
Y. L. Jeyachandran (2015)
Surface nanopatterning of functional polymers by parallel scanning probe lithography
Z. Xie (2015)
Photo-induced crosslinking of water-soluble polymers with a new photobase generator
C. Cao (2010)
Direct write tethered protein arrays
W. E. Schofield (2011)
Fabrication of Nanometer- and Micrometer-Scale Protein Structures by Site-Specific Immobilization of Histidine-Tagged Proteins to Aminosiloxane Films with Photoremovable Protein-Resistant Protecting Groups
Sijing Xia (2016)
Arrays of Individual DNA Molecules on Nanopatterned Substrates
R. Hager (2017)
Channel surface patterning of alternating biomimetic protein combinations for enhanced microfluidic tumor cell isolation.
C. Launiere (2012)
Photocatalytic Lithography with Atomic Layer–Deposited TiO2 Films to Tailor Biointerface Properties
Sofie S T Vandenbroucke (2019)
Site-Selective Oxidative Coupling Reactions for the Attachment of Enzymes to Glass Surfaces through DNA-Directed Immobilization.
Kanwal S Palla (2017)
Modifying biomaterial surfaces to control stem cell growth and differentiation
K. H. Smith (2011)
Fabrication of molecular nanopatterns at aluminium oxide surfaces by nanoshaving of self-assembled monolayers of alkylphosphonates.
Osama el Zubir (2013)
Co- and distinct existence of Tris-NTA and biotin functionalities on individual and adjacent micropatterned surfaces generated by photo-destruction.
A. Biswas (2014)
Smart polymer brush nanostructures guide the self-assembly of pore-spanning lipid bilayers with integrated membrane proteins.
G. D. de Groot (2014)
ESCORTing proteins directly from whole cell-lysate for single-molecule studies
Shwetha Srinivasan (2017)
Chemoselective Immobilization of Proteins by Microcontact Printing and Bio‐orthogonal Click Reactions
Zachary P. Tolstyka (2013)
Light-directed nanosynthesis: near-field optical approaches to integration of the top-down and bottom-up fabrication paradigms.
G. Leggett (2012)
Immobilization of Enzymes to Create Heterogeneous-Enzyme Hybrid Catalysts
Tyler J. Hurlburt (2018)
See more
Semantic Scholar Logo Some data provided by SemanticScholar