Gold Nanoparticle-cytochrome C Complexes: The Effect Of Nanoparticle Ligand Charge On Protein Structure.
Marie-Eve Aubin-Tam, Kimberly Hamad-Schifferli
Published 2005 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
We report the effect of nanoparticle ligand charge on the structure of a covalently, site-specifically linked protein. Au nanoparticles with positive, negative, and neutral ligands were appended to a specific cysteine, C102, of Saccharomyces cerevisiae cytochrome c. Conjugates were purified by HPLC or gel electrophoresis. Circular dichroism spectroscopy shows that changing the nanoparticle ligand dramatically influences the attached cytochrome c structure. The protein retains its structure with neutral ligands but denatures in the presence of charged species. This is rationalized by the electrostatic interaction of amino acids in the local vicinity of C102 with the endgroups of the ligand.
This paper is referenced by
A comparative study of binding properties of different coumarin-based compounds with human serum albumin
Yuanyuan Yue (2018)
Nanoparticle-protein interactions: a thermodynamic and kinetic study of the adsorption of bovine serum albumin to gold nanoparticle surfaces.
Stefano P. Boulos (2013)
Modulating protein amyloid aggregation with nanomaterials.
Bo Wang (2017)
Dynamics of nanoparticle-protein corona: formation, evolution and insight on protein structure
Michele Vitali (2018)
Reversibly pH-responsive gold nanoparticles and their applications for photothermal cancer therapy
Sang-Hak Park (2019)
Molecular Imprinting of Macromolecules for Sensor Applications
Yeşeren Saylan (2017)
Probing the interaction of bovine haemoglobin with gold nanoparticles.
Weimin Yang (2012)
Protein-Nanoparticle Interaction-Induced Changes in Protein Structure and Aggregation.
Yuna Kim (2016)
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
Sebastian Ahlberg (2014)
Cellular uptake, intracellular trafficking, and cytotoxicity of nanomaterials.
Feng Zhao (2011)
Beauty is skin deep: a surface monolayer perspective on nanoparticle interactions with cells and bio-macromolecules.
Krishnendu Saha (2011)
Influence of the alanine side-chain methyl group on the peptide-gold nanoparticles interactions
Mohamed Taha (2020)
Quantifying the nanomachinery of the nanoparticle-biomolecule interface.
Helena de Puig (2011)
Oligothiol graft-copolymer coatings stabilize gold nanoparticles against harsh experimental conditions.
J. S. Kang (2012)
Characterization of lactase-conjugated magnetic nanoparticles
Joey N. Talbert (2013)
Recording and Analyzing Nucleic Acid Distance Distributions with X-Ray Scattering Interferometry (XSI).
Thomas Zettl (2018)
Engineered Protein Polymer-Gold Nanoparticle Hybrid Materials for Small Molecule Delivery
Min Dai (2016)
In silico modeling and experimental evidence of coagulant protein interaction with precursors for nanoparticle functionalization
Chuka Okoli (2013)
Interpreting the adsorption of serum albumin and lactoglobulin onto ZnS nanopaticles: effect of conformational rigidity of the proteins.
Jiban Saikia (2014)
Controlled assemble of erythrosine B/layered double hydroxide ultrathin film: Preparation, fluorescence properties, and photo-response to bovine serum albumin
Lan Jin (2014)
Spectral and molecular modeling studies on the influence of β-cyclodextrin and its derivatives on aripiprazole-human serum albumin binding.
Jin Yan (2015)
Study of the effects of protein corona on nanoparticle-membrane interactions
Abdullah Khan (2013)
Chemical modification of lactase for immobilization on carboxylic acid-functionalized microspheres
Joey N. Talbert (2012)
Highly Dispersed Iron Pyrite Quantum Dots in Volatile Solvent: A Facile Purification Method
Shin-ichi Tamura (2016)
Effect of the protein corona on nanoparticles for modulating cytotoxicity and immunotoxicity
Yeon Kyung Lee (2015)
Measuring the Accessible Surface Area within the Nanoparticle Corona using Molecular Probe Adsorption.
Minkyung Park (2019)
Granulocyte-Colony Stimulating Factor Nanocarriers for Stimulation of the Immune System (Part II): Dose-Dependent Biodistribution and In Vivo Antitumor Efficacy in Combination with Rituximab.
David Kryza (2018)
Electrochemical Analysis of Proteins and Cells
Genxi Li (2012)
β-Cyclodextrin and ultrasound-assisted enzyme renaturation for foam fractionation of laccase from fermentation broth of Trametes hirsuta 18
Yuran Zhang (2020)
Analytical Methods for Characterizing the Nanoparticle–Protein Corona
Anna Laura Capriotti (2014)
Protein-nanoparticle interactions: opportunities and challenges.
Morteza Mahmoudi (2011)
Gold nanorods: their potential for photothermal therapeutics and drug delivery, tempered by the complexity of their biological interactions.
Alaaldin AlKilany (2012)See more