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Antibacterial Activity Of Glutathione-coated Silver Nanoparticles Against Gram Positive And Gram Negative Bacteria.

A. Taglietti, Y. Diaz Fernandez, Elvio Amato, L. Cucca, G. Dacarro, P. Grisoli, V. Necchi, P. Pallavicini, L. Pasotti, M. Patrini
Published 2012 · Medicine, Chemistry
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In the present paper, we study the mechanism of antibacterial activity of glutathione (GSH) coated silver nanoparticles (Ag NPs) on model Gram negative and Gram positive bacterial strains. Interference in bacterial cell replication is observed for both cellular strains when exposed to GSH stabilized colloidal silver in solution, and microbicidal activity was studied when GSH coated Ag NPs are (i) dispersed in colloidal suspensions or (ii) grafted on thiol-functionalized glass surfaces. The obtained results confirm that the effect of dispersed GSH capped Ag NPs (GSH Ag NPs) on Escherichia coli is more intense because it can be associated with the penetration of the colloid into the cytoplasm, with the subsequent local interaction of silver with cell components causing damages to the cells. Conversely, for Staphylococcus aureus, since the thick peptidoglycan layer of the cell wall prevents the penetration of the NPs inside the cytoplasm, the antimicrobial effect is limited and seems related to the interaction with the bacterial surfaces. Experiments on GSH Ag NPs grafted on glass allowed us to elucidate more precisely the antibacterial mechanism, showing that the action is reduced because of GSH coating and the limitation of the translational freedom of NPs.
This paper references
The bactericidal effect of silver nanoparticles
Kateřina Piksová (2010)
10.1016/j.cis.2011.05.008
Silver polymeric nanocomposites as advanced antimicrobial agents: classification, synthetic paths, applications, and perspectives.
Panagiotis Dallas (2011)
10.1039/b604038c
The plasmon band in noble metal nanoparticles: an introduction to theory and applications
Audrey Moores (2006)
10.1177/039139880502801103
Biofilm in Implant Infections: Its Production and Regulation
John William Costerton (2005)
Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically : approved standard
Mary Jane Ferraro (2000)
Resonance (LSPR) of Gold Colloids
M. E. Stewart (2004)
10.1021/la201200r
Synthesis, characterization and antibacterial activity against Gram positive and Gram negative bacteria of biomimetically coated silver nanoparticles.
Elvio Amato (2011)
10.1038/nmat2099
Silver-nanoparticle-embedded antimicrobial paints based on vegetable oil.
A. Kumar (2008)
10.1002/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO;2-3
A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus.
Qing-ling Feng (2000)
10.1016/j.nano.2007.02.001
Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli.
Ahmad Reza Shahverdi (2007)
10.1557/mrs2005.96
Shape-controlled synthesis and surface plasmonic properties of metallic nanostructures
Younan Xia (2005)
10.1016/S0079-6468(08)70024-9
Antimicrobial activity and action of silver.
A. Denver Russell (1994)
10.1021/la104903z
Counterion-induced modulation in the antimicrobial activity and biocompatibility of amphiphilic hydrogelators: influence of in-situ-synthesized Ag-nanoparticle on the bactericidal property.
Sounak Dutta (2011)
10.1021/ES801785M
Toxicity of silver nanoparticles to Chlamydomonas reinhardtii.
Enrique Navarro (2008)
10.1039/B811829A
A direct method for the preparation of glycolipid–metal nanoparticle conjugates: sophorolipids as reducing and capping agents for the synthesis of water re-dispersible silver nanoparticles and their antibacterial activity
Sanjay Singh (2009)
10.1016/j.jcis.2009.07.052
Silver nanoparticle-E. coli colloidal interaction in water and effect on E. coli survival.
Avital Dror-Ehre (2009)
10.1021/bi036231a
Real-time probing of membrane transport in living microbial cells using single nanoparticle optics and living cell imaging.
X. Nancy Xu (2004)
10.1021/bm900620w
Antimicrobial properties of nanostructured hydrogel webs containing silver.
J. Wu (2009)
10.1016/j.tibtech.2005.05.004
Surpassing nature: rational design of sterile-surface materials.
Kim Lewis (2005)
10.1016/J.TSF.2009.03.195
Noble metal nanoparticles for water purification: A critical review
Thalappil Pradeep (2009)
10.1021/es903187s
Binding of silver nanoparticles to bacterial proteins depends on surface modifications and inhibits enzymatic activity.
Nicholas S. Wigginton (2010)
10.5301/IJAO.2011.8784
New Trends in Diagnosis and Control Strategies for Implant Infections
Carla Renata Arciola (2011)
10.1016/j.tibtech.2010.07.006
Nanosilver as a new generation of nanoproduct in biomedical applications.
Karla Chaloupka (2010)
10.1021/cr030063a
Chemistry and properties of nanocrystals of different shapes.
Clemens Burda (2005)
10.1021/cr068126n
Nanostructured plasmonic sensors.
Matthew E. Stewart (2008)
10.1002/cphc.200500113
Synthesis and optical properties of silver nanoparticles and arrays.
David D. Evanoff (2005)
10.1007/s00253-009-2159-5
Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli
Wen-ru Li (2009)
10.1021/jp063826h
Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity.
Aleš Panáček (2006)
10.1016/j.colsurfb.2010.12.020
Comparison of bactericidal activities of silver nanoparticles with common chemical disinfectants.
Karthik R Chamakura (2011)
10.1021/jp0306646
Flashy Silver Nanoparticles
Peter C. Andersen (2004)
10.1021/es1034188
Surface charge-dependent toxicity of silver nanoparticles.
Amro M El Badawy (2011)
10.1016/j.nano.2010.10.005
Recent progress in inorganic and composite coatings with bactericidal capability for orthopaedic applications.
Arash Simchi (2011)
10.1007/s10534-010-9381-6
Antibacterial effect of silver nanoparticles on Staphylococcus aureus
Wen-ru Li (2010)
10.1016/j.jcis.2004.02.012
Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria.
Ivan Sondi (2004)
10.1016/j.jcis.2010.06.019
Self-assembled monolayers of silver nanoparticles firmly grafted on glass surfaces: low Ag+ release for an efficient antibacterial activity.
Piersandro Pallavicini (2010)
10.1021/jp047021q
Solvent and Ligand Effects on the Localized Surface Plasmon Resonance (LSPR) of Gold Colloids
Sujit K Ghosh (2004)
10.1016/S1074-5521(02)00192-8
Antibiofilm approaches: prevention of catheter colonization.
Paul N. Danese (2002)
10.1007/s10646-008-0217-x
What can be inferred from bacterium–nanoparticle interactions about the potential consequences of environmental exposure to nanoparticles?
Andrew L. Neal (2008)
10.1128/aem.02218-06
Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli
Sukdeb Pal (2007)
10.1016/J.APSUSC.2011.02.129
The preparation and antibacterial effects of dopa-cotton/AgNPs
Hong Xu (2011)
10.1016/j.jcis.2008.05.063
Facile preparation and characterization of highly antimicrobial colloid Ag or Au nanoparticles.
Yongwen Zhang (2008)
10.1091/mbc.8.8.1501
High-resolution calcium mapping of the endoplasmic reticulum-Golgi-exocytic membrane system. Electron energy loss imaging analysis of quick frozen-freeze dried PC12 cells.
R Pezzati (1997)
10.1007/S11051-010-9900-Y
A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment
Catalina Marambio-Jones (2010)
10.1128/AEM.71.11.7589-7593.2005
Bactericidal Actions of a Silver Ion Solution on Escherichia coli, Studied by Energy-Filtering Transmission Electron Microscopy and Proteomic Analysis
Mikihiro Yamanaka (2005)
10.1021/ic8002228
Biomolecule-assisted synthesis of water-soluble silver nanoparticles and their biomedical applications.
Qingzhi Wu (2008)
10.1039/b703034g
A practical procedure for producing silver nanocoated fabric and its antibacterial evaluation for biomedical applications.
Hyang Yeon Lee (2007)
10.1128/jb.179.19.6127-6132.1997
Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins.
Xiao-hong Li (1997)
10.1016/S0168-6445(03)00047-0
Bacterial silver resistance: molecular biology and uses and misuses of silver compounds.
Simon Silver (2003)
10.1016/j.cis.2008.09.002
Silver nanoparticles: green synthesis and their antimicrobial activities.
Virender K. Sharma (2009)
10.1021/bm034003q
Biomimetic mineralization of noble metal nanoclusters.
Joseph M. Slocik (2003)
10.1021/mp900056g
Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use.
Jaya Jain (2009)
10.1021/ar7000974
Synthesis of silver nanostructures with controlled shapes and properties.
Benjamin J Wiley (2007)
10.1007/s10295-006-0139-7
Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds
Simon Silver (2006)
10.1021/ac0346609
Biosensing based on light absorption of nanoscaled gold and silver particles.
Filip Frederix (2003)
10.1007/s00253-011-3291-6
Functionalization of biomolecules on nanoparticles: specialized for antibacterial applications
Murugan Veerapandian (2011)
10.1021/bi0508542
Interaction of silver(I) ions with the respiratory chain of Escherichia coli: an electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag+.
Katherine B Holt (2005)
10.1016/j.saa.2008.12.004
Spectroscopic investigation of S-Ag interaction in omega-mercaptoundecanoic acid capped silver nanoparticles.
Suraj Kumar Tripathy (2009)
10.1021/la010536d
Studies on the Reversible Aggregation of Cysteine-Capped Colloidal Silver Particles Interconnected via Hydrogen Bonds
Saikat Mandal (2001)
10.1002/CHIN.200804226
Synthesis of Silver Nanostructures with Controlled Shapes and Properties
Benjamin J Wiley (2008)
10.1007/S11051-008-9428-6
Synthesis and antibacterial activity of silver nanoparticles with different sizes
Gabriel Alejandro Martínez-Castañón (2008)
10.1007/BF00281096
Simultaneous glutaraldehyde-osmium tetroxide fixation with postosmication
Werner W Franke (2004)
A monolayer of a Cu 2 +-tetraazamacrocyclic complex on glass as the adhesive layer for silver nanoparticles grafting , in the preparation of surface-active antibacterial materials w z
Piersandro Pallavicini (2011)
10.1016/j.biotechadv.2008.09.002
Silver nanoparticles as a new generation of antimicrobials.
Mahendra K. Rai (2009)



This paper is referenced by
10.3762/bjnano.11.49
Silver-decorated gel-shell nanobeads: physicochemical characterization and evaluation of antibacterial properties
Marta Bartel (2020)
10.1039/c3dt52242c
Ultrafine silver nanoparticles obtained from ethylene glycol at room temperature: catalyzed by tungstate ions.
J. Li (2014)
10.1039/C7RA00485K
Shell thickness-dependent antibacterial activity and biocompatibility of gold@silver core–shell nanoparticles
Longping Yang (2017)
10.1016/j.msec.2019.110299
Bioreduction mechanism of silver nanoparticles.
Shital Vishnu Sable (2020)
10.3390/ijms20020449
Bactericidal and Cytotoxic Properties of Silver Nanoparticles
Chengzhu Liao (2019)
10.1002/JCTB.5860
New insights into the antimicrobial treatment of water on Ag‐supported solids
Stathis P Theofilou (2019)
10.1002/cyto.a.23055
Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces.
Yuting Guo (2017)
10.1049/iet-nbt.2017.0084
Synthesis of silver nanoparticles from a Desmodium adscendens extract and its antibacterial evaluation on wound dressing material.
Jaya R. Lakkakula (2017)
10.1016/j.jconrel.2017.10.018
In vivo comparisons of silver nanoparticle and silver ion transport after intranasal delivery in mice
J. Falconer (2018)
10.1016/j.msec.2012.12.016
Preparation of AgBr@SiO2 core@shell hybrid nanoparticles and their bactericidal activity.
Yuanyuan Li (2013)
Ecotoxicity of silver nanoparticles on estuarine and coastal bacterial communities
Virginia Echavarri-Bravo (2015)
10.4028/www.scientific.net/JNanoR.25.8
Antioxidant and Antimicrobial Activity of Tellurium Dioxide Nanoparticles Sols
C. L. Zhong (2013)
10.1177/0885328218793488
Tailored antimicrobial activity and long-term cytocompatibility of plasma polymer silver nanocomposites
Steffi Wiesenmueller (2018)
10.1007/978-981-10-6913-0_3
Self-assembled Nanomaterials for Bacterial Infection Diagnosis and Therapy
L. Li (2018)
10.1016/j.ijbiomac.2016.09.052
Antimicrobial and cytotoxicity evaluation of colloidal chitosan - silver nanoparticles - fluoride nanocomposites.
Priscila L L Freire (2016)
10.1186/1477-3155-11-42
Synthesis of well–dispersed silver nanorods of different aspect ratios and their antimicrobial properties against gram positive and negative bacterial strains
Animesh K. Ojha (2013)
10.1002/EJIC.201800709
Self‐Assembled Monolayers of Silver Nanoparticles: From Intrinsic to Switchable Inorganic Antibacterial Surfaces
Piersandro Pallavicini (2018)
10.2147/IJN.S154748
Antimicrobial hydrogels: promising materials for medical application
Kerong Yang (2018)
10.3390/molecules19056737
Synthesis of Silver Nanoparticles by Green Method Stabilized to Synthetic Human Stomach Fluid
Ayman M Atta (2014)
10.1039/c4nr04561k
Bio-NCs--the marriage of ultrasmall metal nanoclusters with biomolecules.
Nirmal Goswami (2014)
10.1007/978-981-10-1008-8_1
Introduction to Nanotheranostics
Subramanian Tamil Selvan (2016)
10.1002/anie.201602965
Engineering Gram Selectivity of Mixed-Charge Gold Nanoparticles by Tuning the Balance of Surface Charges.
Pramod P Pillai (2016)
10.1080/00958972.2020.1739273
Photodynamic antimicrobial chemotherapy of asymmetric porphyrin-silver conjugates towards photoinactivation of Staphylococcus aureus
Samuel M. Shabangu (2020)
10.1016/j.colsurfa.2019.124081
Synthesis and Characterization of Silver Nanoparticles-doped Hydroxyapatite/Alginate Microparticles with Promising Cytocompatibility and Antibacterial Properties
Qiuju Zhou (2020)
Mixing thiols on the surface of silver nanoparticles: preserving antibacterial properties while introducing SERS activity
Amaia Nazabal Ruiz Diaz (2013)
10.1016/j.jcis.2015.12.049
Inhibition of bacterial surface colonization by immobilized silver nanoparticles depends critically on the planktonic bacterial concentration.
Stacy M. Wirth (2016)
10.1039/C7RA13127E
A sensitive LSPR sensor based on glutathione-functionalized gold nanoparticles on a substrate for the detection of Pb2+ ions
Bingbing Feng (2018)
Towards fine-tuning the surface corona of inorganic and organic nanomaterials to control their properties at nano-bio interface
Hemant Kumar Daima (2013)
10.2217/nnm.13.186
Gold nanostars for superficial diseases: a promising tool for localized hyperthermia?
Giuseppe Chirico (2014)
10.1038/s41598-020-64127-9
Antimicrobial activity of a silver-microfibrillated cellulose biocomposite against susceptible and resistant bacteria
J. A. Garza-Cervantes (2020)
10.1016/j.micpath.2016.11.012
Tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against MDR bacteria.
Madhuree Kumari (2017)
10.3390/ijms20061502
Levan-Capped Silver Nanoparticles for Bactericidal Formulations: Release and Activity Modelling
Álvaro González-Garcinuño (2019)
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