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Complete Identification Of Proteins Responsible For Human Blood Plasma Fouling On Poly(ethylene Glycol)-based Surfaces.

T. Riedel, Z. Riedelová-Reicheltová, P. Májek, C. Rodriguez-Emmenegger, M. Houška, Jan E. Dyr, E. Brynda
Published 2013 · Chemistry, Medicine

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The resistance of poly(ethylene glycol) (PEG) against protein adsorption is crucial and has been widely utilized in various biomedical applications. In this work, the complete protein composition of biofilms deposited on PEG-based surfaces from human blood plasma (BP) was identified for the first time using nanoLC-MS/MS, a powerful tool in protein analysis. The mass of deposited BP and the number of different proteins contained in the deposits on individual surfaces decreased in the order of self-assembling monolayers of oligo(ethylene glycol) alkanethiolates (SAM) > poly(ethylene glycol) end-grafted onto a SAM > poly(oligo(ethylene glycol) methacrylate) brushes prepared by surface initiated polymerization (poly(OEGMA)). The BP deposit on the poly(OEGMA) surface was composed only of apolipoprotein A-I, apolipoprotein B-100, complement C3, complement C4-A, complement C4-B, histidine-rich glycoprotein, Ig mu chain C region, fibrinogen (Fbg), and serum albumin (HSA). The total resistance of the surface to the Fbg and HSA adsorption from single protein solutions suggested that their deposition from BP was mediated by some of the other proteins. Current theories of protein resistance are not sufficient to explain the observed plasma fouling. The research focused on the identified proteins, and the experimental approach used in this work can provide the basis for the understanding and rational design of plasma-resistant surfaces.
This paper references
10.1016/j.colsurfb.2010.12.013
Polymeric nanocapsules ultra stable in complex biological media.
C. Rodriguez-Emmenegger (2011)
10.1016/J.BIOMATERIALS.2005.03.010
Polyethylene oxide surfaces of variable chain density by chemisorption of PEO-thiol on gold: adsorption of proteins from plasma studied by radiolabelling and immunoblotting.
L. Unsworth (2005)
10.1186/1479-5876-9-84
Plasma proteome changes in cardiovascular disease patients: novel isoforms of apolipoprotein A1
P. Májek (2011)
Structure of apolipoprotein B-100 in low density lipoproteins.
J. Segrest (2001)
10.1172/JCI118549
Molecular determinants of acute inflammatory responses to biomaterials.
L. Tang (1996)
10.1016/0093-6413(80)90013-0
On the
BENZYL PHTHALATE (1982)
10.1107/S0909049510032450
Protein surface shielding agents in protein crystallization
J. Hasek (2011)
10.1021/LA011365F
Surface-Initiated Polymerizations in Aqueous Media: Effect of Initiator Density
Darren M. Jones (2002)
10.1002/POLA.22180
A structural definition of polymer brushes
W. Brittain (2007)
10.1021/LA981356F
Polymer Brushes that Resist Adsorption of Model Proteins: Design Parameters
A. Halperin (1999)
10.1163/156856200743869
On the molecular basis of fouling resistance
M. Morra (2000)
10.1111/j.1440-1711.2005.01320.x
Histidine‐rich glycoprotein: A novel adaptor protein in plasma that modulates the immune, vascular and coagulation systems
A. Jones (2005)
10.1038/364362B0
Atomic structure and chemistry of human serum albumin
X. M. He (1993)
10.1021/bm101122g
High-resolution visualization of fibrinogen molecules and fibrin fibers with atomic force microscopy.
I. S. Yermolenko (2011)
10.1016/J.BIOMATERIALS.2004.01.023
Biomaterial-associated thrombosis: roles of coagulation factors, complement, platelets and leukocytes.
M. Gorbet (2004)
10.1002/mabi.201200171
Surfaces resistant to fouling from biological fluids: towards bioactive surfaces for real applications.
C. Rodriguez-Emmenegger (2012)
10.1163/156856292X00105
Is the Vroman effect of importance in the interaction of blood with artificial materials?
E. Leonard (1991)
10.1021/JA034820Y
Factors that determine the protein resistance of oligoether self-assembled monolayers --internal hydrophilicity, terminal hydrophilicity, and lateral packing density.
S. Herrwerth (2003)
10.1016/j.colsurfb.2007.11.017
Finding seconds count after contact with blood (and that is all I did).
L. Vroman (2008)
10.1021/LA950646I
Imaging Individual Chaperonin and Immunoglobulin G Molecules with Scanning Tunneling Microscopy
S. Tang (1996)
10.1002/JBM.10117
Adsorption of proteins from infant and adult plasma to biomaterial surfaces.
R. Cornelius (2002)
10.1021/JP972635Z
Molecular Conformation in Oligo(ethylene glycol)-Terminated Self-Assembled Monolayers on Gold and Silver Surfaces Determines Their Ability To Resist Protein Adsorption
P. Harder (1998)
10.1002/adma.200901407
Ultralow-fouling, functionalizable, and hydrolyzable zwitterionic materials and their derivatives for biological applications.
S. Jiang (2010)
Surfaces resistant to fouling from biological fluids : toward biosensors for real applications
C. Rodriguez-Emmenegger (2003)
10.1021/la702310t
Protein-resistant poly(ethylene oxide)-grafted surfaces: chain density-dependent multiple mechanisms of action.
L. Unsworth (2008)
10.1016/j.biomaterials.2010.02.066
Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale
J. Gautrot (2010)
10.1021/la9008569
Ternary protein adsorption onto brushes: strong versus weak.
A. Halperin (2009)
10.1016/S0005-2736(01)00330-3
Protein adsorption to polyethylene glycol modified liposomes from fibrinogen solution and from plasma.
M. E. Price (2001)
10.1161/01.CIR.102.12.1420
Functional Protection by Acute Phase Proteins &agr;1-Acid Glycoprotein and &agr;1-Antitrypsin Against Ischemia/Reperfusion Injury by Preventing Apoptosis and Inflammation
M. Daemen (2000)
10.1016/0021-9797(91)90044-9
Protein—surface interactions in the presence of polyethylene oxide
Seok Hee Jeon (1991)
10.1016/0079-6700(95)00011-4
Blood compatibility of polyethylene oxide surfaces
J. Lee (1995)
10.1021/bm2007086
Poly(ethylene oxide) layers grafted to dopamine-melanin anchoring layer: stability and resistance to protein adsorption.
O. Pop-Georgievski (2011)
10.1021/LA030417T
Interaction of bovine serum albumin and human blood plasma with PEO-tethered surfaces: influence of PEO chain length, grafting density, and temperature.
W. Norde (2004)
10.1021/LA047672D
Protein resistance of surfaces prepared by sorption of end-thiolated poly(ethylene glycol) to gold: effect of surface chain density.
L. Unsworth (2005)
10.1524/ZKSU.2006.SUPPL_23.613
Poly(ethylene glycol) interactions with proteins
J. Hasek (2006)
10.1016/j.biomaterials.2011.10.059
Protein adsorption in three dimensions.
E. Vogler (2012)
10.1002/ADMA.200305830
“Non‐Fouling” Oligo(ethylene glycol)‐ Functionalized Polymer Brushes Synthesized by Surface‐Initiated Atom Transfer Radical Polymerization
Hongwei Ma (2004)
10.1021/JP9716952
MOLECULAR CONFORMATION AND SOLVATION OF OLIGO(ETHYLENE GLYCOL)-TERMINATED SELF-ASSEMBLED MONOLAYERS AND THEIR RESISTANCE TO PROTEIN ADSORPTION
R. Wang (1997)
Blood compatibility of polyethylene oxide
J. H. Lee (1995)
10.1016/S0168-1605(02)00010-7
Spectral surface plasmon resonance biosensor for detection of staphylococcal enterotoxin B in milk.
J. Homola (2002)
10.1021/la900083s
Interaction of blood plasma with antifouling surfaces.
C. Rodriguez Emmenegger (2009)
10.1051/JPHYS:01977003808098300
Adsorption of chain molecules with a polar head a scaling description
S. Alexander (1977)
of biomaterials
A. V. Erwin (2009)
10.1021/LA000340Y
Computer Simulation of Water near the Surface of Oligo(ethylene glycol)-Terminated Alkanethiol Self-Assembled Monolayers†
Alexander J. Pertsin and (2000)
10.1002/3527603824
Polymer brushes : synthesis, characterization, applications
R. Advincula (2004)
10.1002/MABI.201100425
Controlled/living surface-initiated ATRP of antifouling polymer brushes from gold in PBS and blood sera as a model study for polymer modifications in complex biological media.
C. Rodriguez-Emmenegger (2012)
Surfaces resistant to fouling from biological fluids : toward biosensors for real applications
C. Rodriguez-Emmenegger (2003)
10.1021/bm101406m
Substrate-independent approach for the generation of functional protein resistant surfaces.
C. Rodriguez-Emmenegger (2011)
10.1021/LA701007J
Primary versus ternary adsorption of proteins onto PEG brushes.
A. Halperin (2007)
10.1186/1477-5956-9-64
Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia
P. Májek (2011)
10.1016/j.biomaterials.2009.08.016
Volumetric interpretation of protein adsorption: kinetics of protein-adsorption competition from binary solution.
Naris Barnthip (2009)
Computer simulation of water near the surface of oligo ( ethylene glycol ) - terminated alkanethiol self - assembled monolayers
A. J. Pertsin (1991)
10.1016/J.JCIS.2004.08.022
Chemisorption of thiolated poly(ethylene oxide) to gold: surface chain densities measured by ellipsometry and neutron reflectometry.
L. Unsworth (2005)
10.1021/ja2084338
Nanoparticle size and surface chemistry determine serum protein adsorption and macrophage uptake.
Carl D. Walkey (2012)
10.1016/J.JMB.2006.06.009
The structure of bovine complement component 3 reveals the basis for thioester function.
F. Fredslund (2006)
10.1007/978-1-4684-8610-0
Surface and Interfacial Aspects of Biomedical Polymers
J. Andrade (1985)
10.1002/ADMA.200900383
In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins
A. Hucknall (2009)
10.1016/j.biomaterials.2009.07.027
On the nature of biomaterials.
D. F. Williams (2009)
10.1016/0021-9797(91)90043-8
Protein—surface interactions in the presence of polyethylene oxide: I. Simplified theory
S. Jeon (1991)
Apolipoprotein A-I: structure-function relationships.
P. Frank (2000)
10.1002/marc.201100189
Polymer brushes showing non-fouling in blood plasma challenge the currently accepted design of protein resistant surfaces.
C. Rodriguez-Emmenegger (2011)



This paper is referenced by
10.1039/C5NJ01965F
One-pot preparation of boronic acid and PEG bi-functionalized silica particles for separation and purification of catecholamine from rat serum
Y. Wang (2015)
10.1016/J.PORGCOAT.2014.01.027
Recent advances in ATRP methods in relation to the synthesis of copolymer coating materials
P. Król (2014)
10.1021/ACS.MACROMOL.5B02305
Tension Amplification in Tethered Layers of Bottle-Brush Polymers.
Gary M Leuty (2016)
10.1002/mabi.201400470
Polymer brushes interfacing blood as a route toward high performance blood contacting devices.
F. Surman (2015)
10.1038/s41598-018-36597-5
Quantitative design rules for protein-resistant surface coatings using machine learning
Tu C. Le (2019)
Modification of Synthetic Polymeric Materials' Surface for Suppression of Biofilm Formation
Sumita Swar (2019)
10.1016/J.MSER.2019.06.002
The quest for blood-compatible materials: Recent advances and future technologies
Mohammadhasan Hedayati (2019)
10.1021/acs.langmuir.5b02688
Interactions of Apolipoproteins AI, AII, B and HDL, LDL, VLDL with Polyurethane and Polyurethane-PEO Surfaces.
R. Cornelius (2015)
10.1177/1535370216650293
Monocytes and macrophages in tissue repair: Implications for immunoregenerative biomaterial design
M. Ogle (2016)
10.1021/acs.langmuir.6b01852
Platelet Immobilization on Supported Phospholipid Bilayers for Single Platelet Studies.
E. Uhl (2016)
10.1039/C5PY00506J
Synthesis of non-fouling poly[ N -(2-hydroxypropyl)methacrylamide] brushes by photoinduced SET-LRP
M. Vorobii (2015)
10.1016/j.actbio.2018.02.026
The in vitro effects of macrophages on the osteogenic capabilities of MC3T3-E1 cells encapsulated in a biomimetic poly(ethylene glycol) hydrogel.
Leila S. Saleh (2018)
10.1021/acs.chemrev.7b00088
Synthesis, Assembly, and Applications of Hybrid Nanostructures for Biosensing.
S. Zhang (2017)
10.1101/195719
Zwitterionic hydrogels modulate the foreign body response in a modulus-dependent manner
Lauren E. Jansen (2017)
10.1007/s00216-015-8606-5
Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications
Hana Vaisocherová (2015)
10.1039/C3RA43093F
Use of pooled blood plasmas in the assessment of fouling resistance
A. Pereira (2014)
10.1016/j.biomaterials.2014.11.026
Linking the foreign body response and protein adsorption to PEG-based hydrogels using proteomics.
Mark D. Swartzlander (2015)
10.1021/acs.langmuir.7b03048
Neutron Reflectometry Elucidates Protein Adsorption from Human Blood Serum onto PEG Brushes.
Victoria M Latza (2017)
10.1016/J.POLYMER.2016.05.016
Multiple and terminal grafting of linear polyglycidol for surfaces of reduced protein adsorption
Alicja Utrata-Wesołek (2016)
10.1016/j.bios.2013.12.011
Diagnosis of Epstein-Barr virus infection in clinical serum samples by an SPR biosensor assay.
T. Riedel (2014)
10.1016/J.MEMSCI.2015.12.019
A zwitterionic zP(4VP-r-ODA) copolymer for providing polypropylene membranes with improved hemocompatibility
A. Venault (2016)
10.1016/J.SNB.2014.06.075
Hierarchical antifouling brushes for biosensing applications
A. Pereira (2014)
10.1016/j.bios.2016.05.014
Hepatitis B plasmonic biosensor for the analysis of clinical serum samples.
T. Riedel (2016)
10.1021/acsami.7b09725
Highly Specific Binding on Antifouling Zwitterionic Polymer-Coated Microbeads as Measured by Flow Cytometry
E. van Andel (2017)
10.1002/APP.44632
One simple and stable coating of mixed‐charge copolymers on poly(vinyl chloride) films to improve antifouling efficiency
L. Zhang (2017)
10.1021/acs.langmuir.6b02708
Thickness Dependence of Bovine Serum Albumin Adsorption on Thin Thermoresponsive Poly(diethylene glycol) Methyl Ether Methacrylate Brushes by Surface Plasmon Resonance Measurements.
E. Wassel (2016)
10.1002/APP.40518
Functionalization and Hemocompatibility of a Styrenic Thermoplastic Elastomer Based on Its Epoxidized Precursor
Shuaishuai Yuan (2014)
10.1021/ACS.MACROMOL.7B00450
50th Anniversary Perspective: Polymer Brushes: Novel Surfaces for Future Materials
W. Chen (2017)
10.1016/j.biomaterials.2014.12.041
Neutron reflectometry from poly (ethylene-glycol) brushes binding anti-PEG antibodies: evidence of ternary adsorption.
E. Schneck (2015)
10.1016/j.talanta.2020.121483
A new ultralow fouling surface for the analysis of human plasma samples with surface plasmon resonance.
R. D’Agata (2021)
Single and Competitive Protein Sorption at Soft Polymeric Interfaces
Erich Kästner (2016)
10.1021/ACS.ANALCHEM.6B02617
Copolymer Brush-Based Ultralow-Fouling Biorecognition Surface Platform for Food Safety.
Hana Vaisocherová-Lísalová (2016)
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