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Nucleolin Targeting AS1411 Modified Protein Nanoparticle For Antitumor Drugs Delivery.
J. Wu, Chenchen Song, Chenxiao Jiang, Xin Shen, Q. Qiao, Y. Hu
Published 2013 · Chemistry, Medicine
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Over recent years, cell surface nucleolin as an anticancer target has attracted many researchers' attentions. To improve the antitumor efficacy, we developed a nucleolin targeted protein nanoparticle (NTPN) delivery system in which human serum albumin (HSA) was used as drug carrier and a DNA aptamer named AS1411, which had high affinity to nucleolin, was used as a bullet. The HSA nanoparticles (NPs-PTX) were fabricated by a novel self-assembly method and then modified with AS1411 (Apt-NPs-PTX). The resulted Apt-NPs-PTX were spherical. Compared with NPs-PTX, the uptake of Apt-NPs-PTX displayed a significant increase in MCF-7 cells while there was a decrease in nontumor cell lines such as MCF-10A and 3T3 cells. In a cytotoxic study, Apt-NPs-PTX displayed an enhanced cytotoxicity in MCF-7 tumor cells while there was almost no cytotoxicity in MCF-10A cells. Endostatin, a nucleolin inhibitor, could significantly decrease the internalization of Apt-NPs-PTX, suggesting nucleolin mediates the transmembrane process of Apt-NPs-PTX. Therefore, the AS1411 modified NTPN delivery system might be a promising targeted drug delivery system.
This paper references
Molecular switch for the assembly of lipophilic drug incorporated plasma protein nanoparticles and in vivo image.
Guangming Gong (2012)
Paclitaxel in cancer treatment: perspectives and prospects of its delivery challenges.
S. Singh (2009)
In vivo tumor targeting of tumor necrosis factor-alpha-loaded stealth nanoparticles: effect of MePEG molecular weight and particle size.
Chao Fang (2006)
Taxol stabilizes microtubules in mouse fibroblast cells.
P. Schiff (1980)
Development of aptamer therapeutics.
D. Bunka (2010)
Antiproliferative activity of G-quartet-forming oligonucleotides with backbone and sugar modifications.
Virna Dapić (2002)
Nucleolin is a receptor that mediates antiangiogenic and antitumor activity of endostatin.
Hubing Shi (2007)
The amino terminus of mammalian nucleolin specifically recognizes SV40 T-antigen type nuclear localization sequences.
Z. Xue (1993)
Influence of Preparation Methods on Solid State Supersaturation of Amorphous Solid Dispersions: A Case Study with Itraconazole and Eudragit E100
Sandrien Janssens (2010)
Evaluation of two polymeric blends (EVA/PLA and EVA/PEG) as coating film materials for paclitaxel-eluting stent application
X. Liu (2011)
Discovery and development of anticancer aptamers
C. Ireson (2006)
Discovery and development of the G-rich oligonucleotide AS1411 as a novel treatment for cancer.
P. Bates (2009)
Enhanced antitumor efficacy of cisplatin by tirapazamine-transferrin conjugate.
L. Wu (2012)
Relating the phagocytosis of microparticles by alveolar macrophages to surface chemistry: the effect of 1,2-dipalmitoylphosphatidylcholine.
C. Évora (1998)
Effect of surface properties on nanoparticle-cell interactions.
Ayush Verma (2010)
Sheddable Coatings for Long-Circulating Nanoparticles
B. Romberg (2007)
Antiproliferative Activity of G-rich Oligonucleotides Correlates with Protein Binding*
P. Bates (1999)
Aptamer-functionalized PEG-PLGA nanoparticles for enhanced anti-glioma drug delivery.
Jianwei Guo (2011)
Molecular dissection of nucleolin's role in growth and cell proliferation: new insights
M. Srivastava (1999)
Delivering cargoes into cancer cells using DNA aptamers targeting internalized surface portals.
E. Orava (2010)
The cell-surface-expressed nucleolin is associated with the actin cytoskeleton.
A. Hovanessian (2000)
Intraarterial chemotherapy with polyoxyethylated castor oil free paclitaxel, incorporated in albumin nanoparticles (ABI‐007)
B. Damascelli (2001)
Inhibition of DNA Replication and Induction of S Phase Cell Cycle Arrest by G-rich Oligonucleotides*
X. Xu (2001)
Albumin-based nanoparticles as potential controlled release drug delivery systems.
Ahmed O Elzoghby (2012)
Protein nanoparticles as drug carriers in clinical medicine.
M. Hawkins (2008)
Normalization of tumour blood vessels improves the delivery of nanomedicines in a size-dependent manner
Vikash P. Chauhan (2012)
Nonlinear pharmacokinetics and metabolism of paclitaxel and its pharmacokinetic/pharmacodynamic relationships in humans.
L. Gianni (1995)
Phase I trial of cremophor EL with bolus doxorubicin.
MichaelJ. Millward (1998)
Calculation of protein conformation from circular dichroism.
J. Yang (1986)
Taxol: a novel investigational antimicrotubule agent.
E. Rowinsky (1990)
Reversible cell-specific drug delivery with aptamer-functionalized liposomes.
Z. Cao (2009)
Aptamer applications for targeted cancer therapy.
A. Barbas (2010)
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Drug delivery mediated by confined nanosystems: structure-activity relations and factors responsible for the efficacy of formulations
L. Y. Zakharova (2017)
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Shu Han (2019)
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Islam A Hassanin (2020)
Polypod-like structured guanine-rich oligonucleotide aptamer as a selective and cytotoxic nanostructured DNA to cancer cells.
Kohta Mohri (2020)
Organic additives stabilize RNA aptamer binding of malachite green.
Y. Zhou (2016)
Fabrication of aptamer modified TiO2 nanofibers for specific capture of circulating tumor cells.
H. Liu (2020)
AS1411 aptamer-targeted gold nanoclusters effect on the enhancement of radiation therapy efficacy in breast tumor-bearing mice.
Fatemeh Ghahremani (2018)
Aptamers as Therapeutics.
Shahid M. Nimjee (2017)
Targeting Transcription Factor NF-kappa B by Dual Functional Oligodeoxynucleotide Complex for Inhibition of Neuroinflammation
J. Hu (2015)
Aptamer-Mediated Selective Protein Affinity to Improve Scaffold Biocompatibility
L. Parisi (2016)
Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging
Setareh Taki (2019)
Meeting the needs of breast cancer: A nucleolin's perspective.
Ana C. Gregório (2018)
Delivery of thymoquinone to cancer cells with as1411-conjugated nanodroplets
E. M. Murphy (2020)
Combinatorial delivery of superparamagnetic iron oxide nanoparticles (γFe2O3) and doxorubicin using folate conjugated redox sensitive multiblock polymeric nanocarriers for enhancing the chemotherapeutic efficacy in cancer cells.
Chetan Nehate (2017)
Recent advances on the development of pharmacotherapeutic agents on the basis of human serum albumin.
F. Liu (2015)
T1–T2 molecular magnetic resonance imaging of renal carcinoma cells based on nano-contrast agents
J. Li (2018)
Noncovalent tethering of nucleic acid aptamer on DNA nanostructure for targeted photo/chemo/gene therapies.
G. Shim (2019)
Nucleolin-based targeting strategies for cancer therapy: from targeted drug delivery to cytotoxic ligands.
S. Romano (2019)
Multifunctional ATRP based pH responsive polymeric nanoparticles for improved doxorubicin chemotherapy in breast cancer by proton sponge effect/endo-lysosomal escape
Shantanu V. Lale (2015)
One-Step Self-Assembling Method to Prepare Dual-Functional Transferrin Nanoparticles for Antitumor Drug Delivery.
Kaikai Wang (2016)
Applications of Aptamers in Cancer Therapy
Ajda Coker-Gurkan (2018)
The influencing factors and functions of DNA G‐quadruplexes
Wen-Fang Yuan (2020)
Dual-selective photodynamic therapy with a mitochondria-targeted photosensitizer and fiber optic cannula for malignant brain tumors.
Ji Hee Kang (2019)
Relighting Photosensitizers by Synergistic Integration of Albumin and Perfluorocarbon for Enhanced Photodynamic Therapy.
H. Ren (2017)
Investigation of ultrafine gold nanoparticles (AuNPs) based nanoformulation as single conjugates target delivery for improved methotrexate chemotherapy in breast cancer.
Farhat Naz (2019)
Synthesis, characterization and biocompatibility evaluation of iron oxide incorporated magnetic mesoporous silica.
Sakthivel Gandhi (2012)
Nucleolin-targeted selenium nanocomposites with enhanced theranostic efficacy to antagonize glioblastoma.
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