Online citations, reference lists, and bibliographies.
← Back to Search

Biocompatible And Mucoadhesive Bacterial Cellulose-g-poly(acrylic Acid) Hydrogels For Oral Protein Delivery.

N. Ahmad, M. Amin, S. M. Mahali, I. Ismail, V. Chuang
Published 2014 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Stimuli-responsive bacterial cellulose-g-poly(acrylic acid) hydrogels were investigated for their potential use as an oral delivery system for proteins. These hydrogels were synthesized using electron beam irradiation without any cross-linking agents, thereby eliminating any potential toxic effects associated with cross-linkers. Bovine serum albumin (BSA), a model protein drug, was loaded into the hydrogels, and the release profile in simulated gastrointestinal fluids was investigated. Cumulative release of less than 10% in simulated gastric fluid (SGF) demonstrated the potential of these hydrogels to protect BSA from the acidic environment of the stomach. Subsequent conformational stability analyses of released BSA by SDS-PAGE, circular dichroism, and an esterase activity assay indicated that the structural integrity and bioactivity of BSA was maintained and preserved by the hydrogels. Furthermore, an increase in BSA penetration across intestinal mucosa tissue was observed in an ex vivo penetration experiment. Our fabricated hydrogels exhibited excellent cytocompatibility and showed no sign of toxicity, indicating the safety of these hydrogels for in vivo applications.
This paper references
10.1038/nrd2399
Protein therapeutics: a summary and pharmacological classification
B. Leader (2008)
10.1021/la803797x
Physicochemical and conformational studies on BSA-surfactant interaction in aqueous medium.
T. Chakraborty (2009)
10.1002/BIP.20853
Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases.
L. Whitmore (2008)
10.1016/j.carbpol.2013.01.076
Bacterial cellulose and bacterial cellulose-chitosan membranes for wound dressing applications.
W. Lin (2013)
Crosslinked nanofibrillated
S. Spoljaric
10.1155/2013/154856
1-Aryl-3-[4-(thieno[3,2-d]pyrimidin-4-yloxy)phenyl]ureas as VEGFR-2 Tyrosine Kinase Inhibitors: Synthesis, Biological Evaluation, and Molecular Modelling Studies
P. Soares (2013)
10.3109/03639045.2013.819882
Stimuli-responsive bacterial cellulose-g-poly(acrylic acid-co-acrylamide) hydrogels for oral controlled release drug delivery
M. C. I. Mohd Amin (2014)
10.1016/j.carbpol.2013.08.041
Purification, characterization and comparative studies of spray-dried bacterial cellulose microparticles.
M. Amin (2014)
10.1016/J.CARBPOL.2011.04.011
A novel pH-sensitive hydrogel based on dual crosslinked alginate/N-α-glutaric acid chitosan for oral delivery of protein
R. Gong (2011)
10.3934/JIMO.2009.5.127
An optimization approach to the estimation of effective drug diffusivity: From a planar disc into a finite external volume
S. Wang (2008)
Recent advances in bacterial
Y. Huang (2014)
10.1016/j.biomaterials.2011.08.087
A review of the prospects for polymeric nanoparticle platforms in oral insulin delivery.
M. Chen (2011)
Mechanism of solute and drug transport in relaxing , swellable , hydrophilic glassy
R. W. Korsmeyer (1999)
10.1007/s10570-013-0088-z
Recent advances in bacterial cellulose
Y. Huang (2013)
10.1016/J.CARBPOL.2011.12.022
Synthesis and characterization of thermo- and pH-responsive bacterial cellulose/acrylic acid hydrogels for drug delivery
M. Amin (2012)
10.1016/S0939-6411(00)00090-4
Hydrogels in pharmaceutical formulations.
N. Peppas (2000)
10.1155/2013/698141
Biocompatible Bacterial Cellulose-Poly(2-hydroxyethyl methacrylate) Nanocomposite Films
Andrea G. P. R. Figueiredo (2013)
10.1021/MA802429G
Synthesis and Spontaneous Polymerization of Oligo(ethylene glycol)-Conjugated Benzofulvene Macromonomers. A Polymer Brush Forming a Physical Hydrogel
A. Cappelli (2009)
10.1016/j.ijpharm.2012.03.056
Oral delivery of therapeutic protein/peptide for diabetes--future perspectives.
M. Rekha (2013)
10.1002/jps.23385
The biopolymer bacterial nanocellulose as drug delivery system: investigation of drug loading and release using the model protein albumin.
A. Müller (2013)
10.1517/14712598.4.6.881
Hydrogels for oral delivery of therapeutic proteins
N. Peppas (2004)
10.1021/BM060620D
The future prospects of microbial cellulose in biomedical applications.
Wojciech K Czaja (2007)
10.1016/J.ENZMICTEC.2007.10.007
Esterase activity of bovine serum albumin up to 160 °C: A new benchmark for biocatalysis
J. Córdova (2008)
10.2174/138920109788488860
Immunogenicity assessment of therapeutic proteins and peptides.
A. Kaliyaperumal (2009)
10.1016/j.jconrel.2011.01.027
Advances in oral transmucosal drug delivery.
V. F. Patel (2011)
10.1016/j.jconrel.2012.11.005
Polymeric hydrogels for oral insulin delivery.
K. Chaturvedi (2013)
10.1517/14712598.4.1.1
Stem cell therapy for muscular dystrophy
R. Sohn (2004)
10.1016/J.JCONREL.2003.12.022
Oral insulin delivery using P(MAA-g-EG) hydrogels: effects of network morphology on insulin delivery characteristics.
K. Nakamura (2004)
10.1002/JBM.A.31362
Synthesis, characterization, and hydrolytic degradation behavior of a novel biodegradable pH-sensitive hydrogel based on polycaprolactone, methacrylic acid, and poly(ethylene glycol).
Guo Tao Chao (2008)
10.3109/02652048.2011.646329
Novel alginate gel microspheres produced by impinging aerosols for oral delivery of proteins
D. Hariyadi (2012)
10.1016/J.REACTFUNCTPOLYM.2010.10.002
Oral protein delivery: Current status and future prospect
K. Park (2011)
10.1016/j.colsurfb.2011.12.020
Comparison of in vitro methods of measuring mucoadhesion: ellipsometry, tensile strength and rheological measurements.
David Ivarsson (2012)
10.1016/J.POLYMER.2010.12.048
Thermo- and pH-responsive HPC-g-AA/AA hydrogels for controlled drug delivery applications
Z. Zhang (2011)
10.1007/s10570-013-0061-x
Crosslinked nanofibrillated cellulose: poly(acrylic acid) nanocomposite films; enhanced mechanical performance in aqueous environments
S. Spoljaric (2013)
10.1093/nar/gkh371
DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data
L. Whitmore (2004)
10.1016/j.jconrel.2008.11.014
Complexation hydrogels for intestinal delivery of interferon beta and calcitonin.
N. Kamei (2009)
10.1021/cr200157d
Hydrogels for protein delivery.
T. Vermonden (2012)
10.1016/j.biomaterials.2012.02.030
Sustained release of proteins from high water content supramolecular polymer hydrogels.
Eric A. Appel (2012)
10.1007/s11095-009-0045-6
Stability of Protein Pharmaceuticals: An Update
M. Manning (2009)
10.1021/bm2015834
Development of a hybrid dextrin hydrogel encapsulating dextrin nanogel as protein delivery system.
Maria Molinos (2012)
10.1016/j.jconrel.2010.08.007
Cyclodextrin complexed insulin encapsulated hydrogel microparticles: An oral delivery system for insulin.
S. Sajeesh (2010)
10.1021/bm3019664
Thermoresponsive bacterial cellulose whisker/poly(NIPAM-co-BMA) nanogel complexes: synthesis, characterization, and biological evaluation.
Lei Wu (2013)
10.1002/APP.31857
Unique stimuli responsive characteristics of electron beam synthesized bacterial cellulose/acrylic acid composite
N. Halib (2010)
10.1016/j.addr.2012.09.009
Novel crosslinking methods to design hydrogels.
W. Hennink (2002)
Advances in oral transmucosal drug
V. F. Patel (2011)
Hydrogel Drug Delivery: Diffusion Models
Frank Bierbrauer (2005)
10.1016/J.CARBPOL.2010.01.034
Enhanced pH-responsive carrier system based on alginate and chemically modified carboxymethyl chitosan for oral delivery of protein drugs: Preparation and in-vitro assessment
I. ElSherbiny (2010)
10.1016/S0032-3861(98)00546-1
Mechanisms of solute and drug transport in relaxing, swellable, hydrophilic glassy polymers
C. Brazel (1999)
10.1016/0168-3659(87)90035-6
A simple equation for description of solute release II. Fickian and anomalous release from swellable devices
P. L. Ritger (1987)
10.1021/mp400493b
Penetratin derivative-based nanocomplexes for enhanced intestinal insulin delivery.
Xi Zhu (2014)
10.1016/j.drudis.2012.03.019
An overview of natural polymers for oral insulin delivery.
T. Sonia (2012)
10.1016/j.ijpharm.2013.02.030
Approaches for enhancing oral bioavailability of peptides and proteins.
J. Renukuntla (2013)
10.1111/j.1440-1746.2006.04449.x
Gastric emptying time of fluids and solids in healthy subjects determined by 13C breath tests: influence of age, sex and body mass index
S. Hellmig (2006)
10.1016/0378-5173(83)90064-9
Mechanisms of solute release from porous hydrophilic polymers
R. Korsmeyer (1983)
10.1021/bm401580k
Improved stability and antidiabetic potential of insulin containing folic acid functionalized polymer stabilized multilayered liposomes following oral administration.
A. Agrawal (2014)



This paper is referenced by
10.1080/03639045.2016.1257017
Evaluation of superabsorbent linseed-polysaccharides as a novel stimuli-responsive oral sustained release drug delivery system
M. T. Haseeb (2017)
10.2174/1573413714666180723145038
Nanocellulose-based Hydrogels for Biomedical Applications
Amalnath John and Wen Zhong (2019)
10.1016/J.JDDST.2019.101137
In-situ mineralization of calcium carbonate in pectin based edible hydrogel for the delivery of protein at colon
Meenakshi Gautam (2019)
10.1177/0883911519877427
Biocompatibility and adhesive strength properties of poly(methyl acrylate-co-acrylic acid) as a function of acrylic acid content
Chie Yahata (2019)
10.1007/s13346-016-0341-8
Microwaved bacterial cellulose-based hydrogel microparticles for the healing of partial thickness burn wounds
M. Pandey (2016)
10.1517/17425247.2016.1160889
Enhancement of oral insulin bioavailability: in vitro and in vivo assessment of nanoporous stimuli-responsive hydrogel microparticles
N. Ahmad (2016)
10.1016/J.COLSURFA.2019.06.027
Pectin/PEG food grade hydrogel blend for the targeted oral co-delivery of nutrients
Meenakshi Gautam (2019)
10.1016/J.MATTOD.2018.02.001
Nanocellulose as a natural source for groundbreaking applications in materials science: Today’s state
D. Klemm (2018)
10.1007/978-3-030-12919-4_17
Nanocellulose Composite Biomaterials in Industry and Medicine
O. Shoseyov (2019)
10.1016/j.carbpol.2016.05.029
Advances in biomedical and pharmaceutical applications of functional bacterial cellulose-based nanocomposites.
H. Ullah (2016)
10.1016/j.ijbiomac.2020.01.280
Pinus residue/pectin-based composite hydrogels for the immobilization of β-D-galactosidase.
M. A. Cargnin (2020)
10.1002/CPLU.201600112
Biodegradable Polysaccharides for Controlled Drug Delivery.
Janarthanan Pushpamalar (2016)
10.1007/s10570-017-1202-4
Fabrication, characterization and evaluation of bacterial cellulose-based capsule shells for oral drug delivery
H. Ullah (2017)
10.1007/s00253-019-09966-x
Microbial gums: introducing a novel functional component of edible coatings and packaging
Mahmood Alizadeh-Sani (2019)
10.1039/C6RA16746B
Physically cross-linked pH-responsive chitosan-based hydrogels with enhanced mechanical performance for controlled drug delivery
Yuju Che (2016)
10.1177/0885328220912843
Fabrication and characterization of a novel semi-interpenetrating network hydrogel based on sodium carboxymethyl cellulose and poly(methacrylic acid) for oral insulin delivery
Shun-ying Li (2020)
10.1016/J.CARBPOL.2019.115210
Polysaccharides constructed hydrogels as vehicles for proteins and peptides. A review.
E. Dragan (2019)
10.1080/17425247.2018.1504017
Recent advances in oral delivery of biologics: nanomedicine and physical modes of delivery
D. Vllasaliu (2018)
10.1007/s13273-017-0028-3
The role of bacterial cellulose in artificial blood vessels
S. Lee (2017)
10.1007/978-3-319-25322-0_1
Polymeric Hydrogels: A Review of Recent Developments
S. Mishra (2016)
10.1016/j.jcis.2019.10.118
Protective properties of mesocellular silica foams against aggregation and enzymatic hydrolysis of loaded proteins for oral protein delivery.
Ye He (2019)
10.1517/17425247.2015.997707
Recent advances in the role of supramolecular hydrogels in drug delivery
M. Amin (2015)
10.3390/molecules25071539
New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers
C. Vasile (2020)
10.1016/j.ijpharm.2017.12.036
Microparticles, microcapsules and microspheres: A review of recent developments and prospects for oral delivery of insulin.
Chun Y Wong (2018)
10.1201/9781315152271-8
Porous Hydrogels as Carrier for Delivery of Macromolecular Drugs
Ecaterina Stela Dragan (2017)
10.3389/fchem.2020.00050
Chitosan/Xanthan Gum Based Hydrogels as Potential Carrier for an Antiviral Drug: Fabrication, Characterization, and Safety Evaluation
Nadia Malik (2020)
10.1007/s13346-017-0450-z
Pluronic-F127 composite film loaded with erythromycin for wound application: formulation, physicomechanical and in vitro evaluations
Taradokht Alavi (2017)
10.3390/pr8050624
Bacterial Cellulose as a Versatile Platform for Research and Development of Biomedical Materials
S. Gorgieva (2020)
10.1007/s10570-016-0986-y
Applications of bacterial cellulose in food, cosmetics and drug delivery
H. Ullah (2016)
10.1039/C8TB02331J
Multifunctional cellulose-based hydrogels for biomedical applications.
Lian-Hua Fu (2019)
10.1016/j.molliq.2020.112865
Characterization of the interaction of diosgenin with human serum albumin and α1-acid glycoprotein using biophysical and bioinformatic tools
Khairul Azreena Bakar (2020)
10.1039/C4RA16103C
Modified biopolymer-dextrin based crosslinked hydrogels: application in controlled drug delivery
D. Das (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar