Online citations, reference lists, and bibliographies.
← Back to Search

Enhanced Oral Uptake Of Tomato Lectin-Conjugated Nanoparticles In The Rat

N. Hussain, P. U. Jani, A. Florence
Published 2004 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
AbstractPurpose. To investigate the usefulness of a surface-conjugated, bioadhesive molecule, tomato lectin, to augment intestinal uptake of orally administered inert nanoparticles. Methods. Fluorescent 500 nm polystyrene nanoparticles with tomato lectin covalently surface coupled using a carbodiimide reaction were administered to female Wistar rats by oral gavage daily for 5 days. Results. Analysis of tissue extracted polymer by gel permeation chromatography revealed a 23% systemic uptake of tomato lectin conjugated nanoparticles compared to < 0.5% of TL nanoparticles blocked with N-acetylchitotetraose thus representing an increase of almost 50 fold across the intestine. Intestinal uptake of tomato lectin-conjugated nanoparticles via the villous tissue was 15 times higher than uptake by the gut-associated lymphoid tissue. Conclusions. The application of tomato lectin as a bioadhesive agent in vivo has been demonstrated to enhance subsequent intestinal transcytosis of colloidal particulates to which it is bound.
This paper references
Further histological evidence of the gastrointestinal absorption of polystyrene nanospheres in the rat
P. U. Jani (1992)
Virulence Mechanisms of Bacterial Pathogens
J. Roth (1995)
The Uptake and Translocation of Latex Nanospheres and Microspheres after Oral Administration to Rats
P. Jani (1989)
Obstacles to Polypeptide Delivery
L. A. Sternson (1987)
Nanoparticle Uptake by the Rat Gastrointestinal Mucosa: Quantitation and Particle Size Dependency
P. Jani (1990)
Cytochemical analysis of alkaline phosphatase and esterase activities and of lectin-binding and anionic sites in rat and mouse Peyer's patch M cells.
R. L. Owen (1983)
In vitro bioadhesion of carbohydrate-containing N-(2-hydroxypropyl) methacrylamide copolymers to the GI tract of guinea pigs
B. Říhová (1992)
The laboratory rat
M. Suckow (2006)
Uptake and translocation of fluorescent latex particles by rabbit Peyer's patch follicle epithelium: a quantitative model for M cell uptake.
J. Pappo (1989)
Tomato lectin resists digestion in the mammalian alimentary canal and binds to intestinal villi without deleterious effects
D. Kilpatrick (1985)
Preparation and characterization of lectin-latex conjugates for specific bioadhesion.
J. M. Irache (1994)
Uptake of Phosphatidylserine Liposomes by Rat Peyer's Patches Following Intraluminal Administration
H. Tomizawa (2004)
Monoclonal antibody-directed targeting of fluorescent polystyrene microspheres to Peyer's patch M cells.
J. Pappo (1991)
Rapid insorption of small particles in the gut.
W. Sass (1990)
Pinocytosis by epithelium associated with lymphoid follicles in the bursa of Fabricius, appendix, and Peyer's patches. An electron microscopic study.
D. Bockman (1973)
A study of particulate intestinal absorption and hepatocellular uptake. Use of polystyrene latex particles.
E. Sanders (1961)
Lectins in the U.S. Diet. Isolation and characterization of a lectin from the tomato (Lycopersicon esculentum).
M. Nachbar (1980)
Uptake of tomato lectin by the adult rat small intestine in vitro
B. Naisbett (1989)

This paper is referenced by
Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles
A. P. Walczak (2015)
In vitro assays for hazard identification of nanoparticles
I. Rietjens (2011)
Microparticles as vaccine adjuvants and delivery systems
D. O'hagan (2003)
Presence of microplastics and nanoplastics in food, with particular focus on seafood EFSA Panel on Contaminants in the Food Chain (CONTAM)
T. Schwerdtle (2016)
How to design the surface of peptide-loaded nanoparticles for efficient oral bioavailability?
H. Malhaire (2016)
Factors Affecting the Oral Bioavailability of Nanomaterials
Georgia K. Hinkley (2014)
Presence of microplastics and nanoplastics in food, with particular focus on seafood
Lap Hoogenboom (2016)
Chromium nanoparticle exhibits higher absorption efficiency than chromium picolinate and chromium chloride in Caco-2 cell monolayers.
L-Y Zha (2008)
Preparation and evaluation of lectin-conjugated PLGA nanoparticles for oral delivery of thymopentin.
Yashu Yin (2006)
Nanoparticles for Oral Delivery of Insulin
A. Ribeiro (2013)
Polymeric nanoparticle drug delivery technologies for oral delivery applications
Eric M. Pridgen (2015)
In vitro evaluation of biodegradable microspheres with surface-bound ligands.
Mark E. Keegan (2006)
Nanoparticles in Different Delivery Systems: A Brief Review
Anubhav Nagal (2013)
Lipid-core nanocapsules restrained the indomethacin ethyl ester hydrolysis in the gastrointestinal lumen and wall acting as mucoadhesive reservoirs.
V. Cattani (2010)
PEGylation for enhancing nanoparticle diffusion in mucus☆
Justin T Huckaby (2018)
Matrix liposomes: A solid liposomal formulation for oral administration
S. Pantze (2014)
Considering food matrix and gastrointestinal effects in enhancing bioactive peptide absorption and bioavailability
X. Sun (2020)
Bioavailability and biodistribution of differently charged polystyrene nanoparticles upon oral exposure in rats
A. P. Walczak (2015)
Bioadhesive microdevices with multiple reservoirs: a new platform for oral drug delivery.
A. Ahmed (2002)
Ligand-Mediated Gastrointestinal Targeting
J. Woodley (2002)
Lectins as Bioactive Plant Proteins: A Potential in Cancer Treatment
E. D. de Mejia (2005)
Nanotechnology as Emerging Tool for Enhancing Solubility of Poorly Water-Soluble Drugs
S. Kumar (2012)
Lectin-coated particulate delivery systems for oral administration
Catarina Pinto Reis (2016)
Binding and Uptake of Wheat Germ Agglutinin-Grafted PLGA-Nanospheres by Caco-2 Monolayers
A. Weissenboeck (2004)
Generation of improved mucosal vaccines by induction of innate immunity
E. Lavelle (2005)
Microplastics in fisheries and aquaculture
Amy Lusher (2017)
Systemic Absorption of Nanomaterials by Oral Exposure: Part of the ”Better control of nano” initiative 2012-2015
Mona-lise Binderup (2013)
Biopharmaceutical parameters to consider in order to alter the fate of nanocarriers after oral delivery.
E. Roger (2010)
Current methods for attaching targeting ligands to liposomes and nanoparticles.
Leila Nobs (2004)
Iron oxide/hydroxide nanoparticles with negatively charged shells show increased uptake in Caco-2 cells.
Markus R Jahn (2012)
Polymeric Nano/Microparticles for Oral Delivery of Proteins and Peptides
S. Sajeesh (2015)
Food Nanoparticles and Intestinal Inflammation: A Real Risk?
Alina Martirosyan (2012)
See more
Semantic Scholar Logo Some data provided by SemanticScholar