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Three-Dimensional Photopatterning Of Hydrogels Containing Living Cells

Valerie A. Liu, S. Bhatia
Published 2002 · Materials Science

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Recent advances in tissue engineering have leveraged progress in both polymer chemistry and cell biology. For example, photopolymerizable biomaterials have been developed that can be used to photoencapsulate cells in peptide-derivatized hydrogel networks. While these materials have been useful in bone, cartilage and vascular tissue engineering, they have limited applicability to more complex tissues that are characterized by precise cell and tissue organization (e.g., liver, kidney). Typically, the tissue shape has been defined solely by the container used for photopolymerization. In this paper, we describe the use of photolithographic techniques to broaden the capability of photopolymerizable PEG-based biomaterials by inclusion of structural features within the cell/hydrogel network. Specifically, we describe the development of a photopatterning technique that allows localized photoencapsulation of live mammalian cells to control the tissue architecture. In this study, we optimized the effect of ultraviolet (UV) exposure and photoinitiator concentration on both photopatterning resolution and cell viability. With regard to photopatterning resolution, we found that increased UV exposure broadens feature size, while photoinitiator concentration had no significant effect on patterning resolution. Cell viability was characterized using HepG2 cells, a human hepatoma cell line. We observed that UV exposure itself did not cause cell death over the doses and time scale studied, while the photoinitiator 2,2-dimethoxy-2-phenyl-acetophenone was itself cytotoxic in a dose-dependent manner. Furthermore, the combination of UV and photoinitiator was the least biocompatible condition presumably due to formation of toxic free radicals. The utility of this method was demonstrated by photopatterning hydrogels containing live cells in various single layer structures, patterns of multiple cellular domains in a single “hybrid” hydrogel layer, and patterns of multiple cell types in multiple layers simulating use in a tissue engineering application. The combination of microfabrication approaches with photopolymerizable biomaterials will have implications in tissue engineering, elucidating fundamental structure–function relationships of tissues, and formation of immobilized cell arrays for biotechnological applications.
This paper references
Biomed. Mater. Res
J H Ward (2001)
Characterization of permeability and network structure of interfacially photopolymerized poly(ethylene glycol) diacrylate hydrogels.
G. Cruise (1998)
Cell migration through defined, synthetic extracellular matrix analogues
A. Gobin (2002)
Release of protein from highly cross-linked hydrogels of poly(ethylene glycol) diacrylate fabricated by UV polymerization.
M. Mellott (2001)
pH-sensitive thin hydrogel microfabricated by photolithography
G. Chen (1998)
Eur. J. Pharm. Biopharm
N A Peppas (2000)
Journal of Biomedical Materials Research
D L Hern (1998)
Novel cell immobilization method utilizing centrifugal force to achieve high-density hepatocyte culture in porous scaffold.
T. H. Yang (2001)
Ann. N.Y. Acad. Sci
L G Grif®th (1997)
Micropatterning of biomedical polymer surfaces by novel UV polymerization techniques.
J. H. Ward (2001)
Hydrogels in pharmaceutical formulations.
N. Peppas (2000)
Functional hydrogel structures for autonomous flow control inside microfluidic channels
D. Beebe (2000)
In Vitro Organogenesis of Liver Tissue a
L. Griffith (1997)
Microtubular architecture of biodegradable polymer scaffolds.
P. Ma (2001)
G Chen (1998)
Biomed. Mater. Res
T H Yang (2001)
Fundamentals of microfabrication
M. Madou (1997)
Photoencapsulation of chondrocytes in poly(ethylene oxide)-based semi-interpenetrating networks.
J. Elisseeff (2000)
Tethered-TGF-β increases extracellular matrix production of vascular smooth muscle cells
B. Mann (2001)
Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing.
D. Hern (1998)
Faseb J
A S Gobin (2002)
Biomedical Microdevices
S N Bhatia (1999)
Controlled growth factor release from synthetic extracellular matrices
K. Lee (2000)
A sensitivity study of the key parameters in the interfacial photopolymerization of poly(ethylene glycol) diacrylate upon porcine islets.
G. Cruise (1998)
Exp. Hematol
S F Badylak (2001)
Biomed. Mater. Res
S J Bryant (2002)
Tissue engineering : Frontiers in biotechnology
R. Langer (1993)
Marrow-derived cells populate scaffolds composed of xenogeneic extracellular matrix.
S. Badylak (2001)
Microfabrication of hydrogels as polymer scaffolds for tissue engineering applications
T.Yu Tyu (2000)
Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering.
B. Mann (2001)
Effect of cell–cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells
S. Bhatia (1999)
Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro
S. Bryant (2000)
Faseb Journal
S N Bhatia (1999)
Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels.
S. Bryant (2002)
J. Biomater. Sci. Polym. Ed
S J Bryant (2000)
Biomed. Mater. Res
P X Ma (2001)
Polymer Preprints
T Yu (2000)
Journal of Biomedical Materials Research
J Elisseeff (2000)

This paper is referenced by
Tissue engineering: current state and perspectives
E. Lavik (2004)
2 3-D Fabrication Technology for Tissue Engineering
A. A. Chen (2007)
Chapter 14 – Scaffold design and fabrication
D. Hutmacher (2008)
Microscale Strategies for Generating Cell-Encapsulating Hydrogels.
Šeila Selimović (2012)
Microscopic origin of the rheological and surface properties of embryonic cell aggregates
Tomita Vasilica Stirbat (2012)
Effect of the glass surface modification on the strength of methacrylate monolith attachment.
Jana Vidič (2005)
Multilayer vascular grafts based on collagen-mimetic proteins.
M. B. Browning (2012)
Image-predicated sorting of adherent cells using photopatterned hydrogels.
J. Kovac (2013)
Hydrogels for in vivo‐like three‐dimensional cellular studies
R. Devolder (2012)
An extracellular matrix microarray for probing cellular differentiation
C. Flaim (2005)
Rapid 3D printing of anatomically accurate and mechanically heterogeneous aortic valve hydrogel scaffolds.
L. Hockaday (2012)
Generation of spatially aligned collagen fiber networks through microtransfer molding.
N. Naik (2014)
Fabrication of PEG Hydrogel Micropatterns by Soft-Photolithography and PEG Hydrogel as Guided Bone Regeneration Membrane in Dental Implantology
K. Subramani (2012)
A photolithographic method to create cellular micropatterns.
J. Karp (2006)
3D Printed Hydrogel Micro-Environments And Bioreactor Conditioning To Develop Native Heterogeneity In Tissue Engineered Heart Valves
L. Hockaday (2014)
A Simple Pipetting-based Method for Encapsulating Live Cells into Multi-layered Hydrogel Droplets
J. Yeon (2018)
Fabrication of three-dimensional tissues.
V. Tsang (2007)
Stem cell bioprocessing: fundamentals and principles
Mark R Placzek (2008)
A tunable hydrogel system and pulsatile flow bioreactor for the development of tissue engineered vascular grafts
Melissa K. McHale (2009)
Smart systems for tissue engineering: combining natural and synthetic origin polymers
J. Magalhães (2011)
Low-Dose, Long-Wave UV Light Does Not Affect Gene Expression of Human Mesenchymal Stem Cells
Darice Y. Wong (2015)
Photocrosslinkable Materials for the Fabrication of Tissue-Engineered Constructs by Stereolithography
R. Pereira (2014)
Microsoft Word - Table of Contents_nicu
Engineering tissues for in vitro applications.
S. Khetani (2006)
Microengineered hydrogels for tissue engineering.
A. Khademhosseini (2007)
Mechanical feasibility of ABS/HIPS-based multi-material structures primed by low-cost polymer printer
S. Singh (2019)
A Simple Three-dimensional Hydrogel Platform Enables Ex Vivo Cell Culture of Patient and PDX Tumors for Assaying Their Response to Clinically Relevant Therapies
K. Hribar (2019)
Tissue regeneration: from synthetic scaffolds to self-organizing morphogenesis.
T. Chen (2014)
Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network
S. Morelli (2012)
Laser-Guided Assembly of Heterotypic 3 D Living Cell Microarrays
G. M. Akselrod (2005)
Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications
M. Pinto (2012)
Design and fabrication of an artificial cornea based on a photolithographically patterned hydrogel construct
D. Myung (2007)
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