Online citations, reference lists, and bibliographies.
← Back to Search

Tumour Amplified Kinase STK15/BTAK Induces Centrosome Amplification, Aneuploidy And Transformation

H. Zhou, J. Kuang, L. Zhong, W. Kuo, J. Gray, A. Sahin, B. Brinkley, S. Sen
Published 1998 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
The centrosomes are thought to maintain genomic stability through the establishment of bipolar spindles during cell division, ensuring equal segregation of replicated chromosomes to two daughter cells. Deregulated duplication and distribution of centrosomes have been implicated in chromosome segregation abnormalities, leading to aneuploidy seen in many cancer cell types. Here, we report that STK15 (also known as BTAK and aurora2), encoding a centrosome-associated kinase, is amplified and overexpressed in multiple human tumour cell types, and is involved in the induction of centrosome duplication-distribution abnormalities and aneuploidy in mammalian cells. STK15 amplification has been previously detected in breast tumour cell lines and in colon tumours; here, we report its amplification in approximately 12% of primary breast tumours, as well as in breast, ovarian, colon, prostate, neuroblastoma and cervical cancer cell lines. Additionally, high expression of STK15 mRNA was detected in tumour cell lines without evidence of gene amplification. Ectopic expression of STK15 in mouse NIH 3T3 cells led to the appearance of abnormal centrosome number (amplification) and transformation in vitro. Finally, overexpression of STK15 in near diploid human breast epithelial cells revealed similar centrosome abnormality, as well as induction of aneuploidy. These findings suggest that STK15 is a critical kinase-encoding gene, whose overexpression leads to centrosome amplification, chromosomal instability and transformation in mammalian cells.
This paper references
The Origin of Malignant Tumours
R. DeFries (1929)
10.1073/PNAS.81.14.4439
Phosphoproteins are components of mitotic microtubule organizing centers.
D. Vandré (1984)
Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10.
Herbert D. Soule (1990)
10.1083/JCB.112.6.1177
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein
F. Verde (1991)
10.1083/JCB.116.6.1431
Regulation of the microtubule nucleating activity of centrosomes in Xenopus egg extracts: role of cyclin A-associated protein kinase
B. Buendía (1992)
10.1128/MCB.14.7.4731
Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation.
L. Francisco (1994)
10.1073/PNAS.91.6.2156
Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization.
A. Kallioniemi (1994)
10.1016/0092-8674(95)90142-6
Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo
A. Blangy (1995)
10.1096/fasebj.9.8.7768349
The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification 1
S. Hanks (1995)
Comparative genomic in situ hybridization of colon carcinomas with replication error.
J. Schlegel (1995)
10.1016/0092-8674(95)90374-7
Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles
D. Glover (1995)
10.1006/EXCR.1995.1373
Cell-cycle-regulated localization of tyrosine and threonine phosphoepitopes at the kinetochores of mitotic chromosomes.
S. Taagepera (1995)
Genetic analysis of benign, low-grade, and high-grade ovarian tumors.
H. Iwabuchi (1995)
10.1091/MBC.6.12.1673
CP60: a microtubule-associated protein that is localized to the centrosome in a cell cycle-specific manner.
D. Kellogg (1995)
Genetic aberrations detected by comparative genomic hybridization predict outcome in node-negative breast cancer.
J. Isola (1995)
FASEB Protein kinases 6 . The eukaryotic protein kinase superfamily : kinase ( catalytic ) domain structure and classification
D. M. Glover (1995)
a microtubule-associated protein that is localized to the centrosome in a cell cyclespecific manner
D. R. Kellogg (1995)
CP 60 : a microtubule - associated protein that is localized to the centrosome in a cell cyclespecific manner
D. D. Vandre (1995)
kinase (catalytic) domain structure and classification
S. K. Hanks (1995)
10.1126/science.271.5256.1744
Abnormal Centrosome Amplification in the Absence of p53
K. Fukasawa (1996)
Mapping of chromosomal imbalances in pancreatic carcinoma by comparative genomic hybridization.
S. Solinas-Toldo (1996)
10.1083/JCB.135.6.1701
Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plk1) in the functional maturation of mitotic centrosomes
H. Lane (1996)
10.1055/S-2007-997605
Genetisches Screening von Kopf-Hals-Karzinomen mittels der Komparativen Genomischen Hybridisierung (CGH)
U. Bockmuehl (1996)
[Genetic screening of head-neck carcinomas using comparative genomic hybridization (CGH)].
U. Bockmuehl (1996)
A molecular genetic model of human bladder cancer pathogenesis.
C. Reznikoff (1996)
10.1038/sj.onc.1201144
ayk1, a novel mammalian gene related to Drosophila aurora centrosome separation kinase, is specifically expressed during meiosis
A. Yanai (1997)
10.1038/sj.onc.1200862
Prognostic significance of polo-like kinase (PLK) expression in non-small cell lung cancer
G. Wolf (1997)
10.1083/JCB.138.3.643
A Novel Mammalian, Mitotic Spindle–associated Kinase Is Related to Yeast and Fly Chromosome Segregation Regulators
G. Gopalan (1997)
Gains, losses, and amplifications of DNA sequences evaluated by comparative genomic hybridization in chondrosarcomas.
M. Larramendy (1997)
10.1038/sj.onc.1201065
A putative serine/threonine kinase encoding gene BTAK on chromosome 20q13 is amplified and overexpressed in human breast cancer cell lines
S. Sen (1997)
10.1006/BBRC.1997.6633
Malignant transformation of mammalian cells initiated by constitutive expression of the polo-like kinase.
M. Smith (1997)
10.1074/JBC.272.21.13766
Cell Cycle-dependent Expression and Spindle Pole Localization of a Novel Human Protein Kinase, Aik, Related to Aurora of Drosophila and Yeast Ipl1*
M. Kimura (1997)
10.1038/32520
A checkpoint on the road to cancer
T. Orr-Weaver (1998)
10.1038/32688
Mutations of mitotic checkpoint genes in human cancers
D. Cahill (1998)
10.1038/1727
Trisomy 7-harbouring non-random duplication of the mutant MET allele in hereditary papillary renal carcinomas
Z. Zhuang (1998)
10.1038/sj.onc.1201890
Expression of a p53 mutant in the epidermis of transgenic mice accelerates chemical carcinogenesis
X. Wang (1998)
10.1093/emboj/17.2.470
A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators
Andrew M. Fry (1998)
10.1073/PNAS.95.6.2950
Centrosome hypertrophy in human breast tumors: implications for genomic stability and cell polarity.
W. Lingle (1998)
10.1093/emboj/17.11.3052
A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers
James R. Bischoff (1998)



This paper is referenced by
10.1016/j.neo.2021.05.003
The synergy of BET inhibitors with aurora A kinase inhibitors in MYCN-amplified neuroblastoma is heightened with functional TP53
Joanna S. Yi (2021)
10.1101/2021.03.21.436300
Identification of regulatory networks associated with anti-HIV/AIDS genes via integrated transcriptome, epigenome and proteome analyses
Gexin Liu (2021)
10.3390/cancers13081916
Targeting Post-Translational Modifications of the p73 Protein: A Promising Therapeutic Strategy for Tumors
Z. Omran (2021)
10.1016/j.cancergen.2021.01.011
TCGA dataset screening for genes implicated in endometrial cancer using RNA-seq profiling.
Xiaoli Fu (2021)
10.3390/ijms22041936
OTUD6A Is an Aurora Kinase A-Specific Deubiquitinase
H. J. Kim (2021)
10.1038/s41419-021-03894-3
ISL1 promoted tumorigenesis and EMT via Aurora kinase A-induced activation of PI3K/AKT signaling pathway in neuroblastoma
Mengzhen Li (2021)
10.1038/s41388-021-01766-w
Nuclear localisation of Aurora-A: its regulation and significance for Aurora-A functions in cancer
F. D. Naso (2021)
10.1002/1878-0261.13025
Reversing tozasertib resistance in glioma through inhibition of pyruvate dehydrogenase kinases.
E. Jane (2021)
10.7150/ijms.58516
The impact of Aurora kinase A genetic polymorphisms on cervical cancer progression and clinicopathologic characteristics
Pei-Ju Wu (2021)
10.1101/2021.03.10.433854
A Focused Small-Molecule Screen Identifies PHA-680626 as an Amphosteric Inhibitor Disrupting the Interaction between Aurora-A and N-Myc
Fani Souvalidou (2021)
10.7150/ijms.61969
Simultaneous silencing Aurora-A and UHRF1 inhibits colorectal cancer cell growth through regulating expression of DNMT1 and STAT1
Jing Han (2021)
10.1177/1535370221991094
Mitotic kinases as drivers of the epithelial-to-mesenchymal transition and as therapeutic targets against breast cancers
Stephanie Colón-Marrero (2021)
10.1016/j.biochi.2020.12.024
Heteroarene-fused anthraquinone derivatives as potential modulators for human aurora kinase B.
Mandeep Singh (2021)
10.1007/978-3-030-64668-4_7
Diagnosis of Colorectal Cancer Using Molecular Techniques
Unnati Bhalerao (2021)
10.3389/fonc.2021.643620
Cytoskeleton Dynamics in Peripheral T Cell Lymphomas: An Intricate Network Sustaining Lymphomagenesis
V. Fragliasso (2021)
10.1186/s12863-020-00843-5
Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse
Bei Li (2020)
10.1038/s41375-020-0921-y
Genome instability in multiple myeloma
C. Neuse (2020)
The role of Aurora-A in human cancers and future therapeutics.
Xinrong Lin (2020)
10.1038/s41388-020-1296-2
Genome-wide CRISPR screen uncovers a synergistic effect of combining Haspin and Aurora kinase B inhibition
M. Huang (2020)
10.3389/fonc.2020.01040
AURKA rs2273535 T>A Polymorphism Associated With Cancer Risk: A Systematic Review With Meta-Analysis
Shujie Wang (2020)
10.1038/s41589-020-00652-y
PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase
B. Adhikari (2020)
10.1186/s12860-020-00327-5
The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture
A. Bresch (2020)
10.1007/s00109-020-01895-x
The therapeutic potential of Aurora kinases targeting in glioblastoma: from preclinical research to translational oncology
Taciani de Almeida Magalhães (2020)
10.1080/07391102.2020.1772109
Targeting the protein-protein interface pocket of Aurora-A-TPX2 complex: rational drug design and validation
V. Bhardwaj (2020)
10.34172/AJMB.2020.15
Identifying Potential Biomarkers in Colorectal Cancer and Developing Non-invasive Diagnostic Models Using Bioinformatics Approaches
M. Saidijam (2020)
10.1007/978-981-15-8179-3_2
Epigenetic Regulator Enzymes and Their Implications in Distinct Malignancies
Shabir Ahmad Ganai (2020)
10.1016/j.bmc.2020.115740
Design and synthesis of Coenzyme A analogues as Aurora kinase A inhibitors: An exploration of the roles of the pyrophosphate and pantetheine moieties.
F. Bellany (2020)
10.1016/j.semcdb.2020.04.019
Centrosome dysfunction in human diseases.
S. Jaiswal (2020)
10.3892/or.2020.7526
Identification of lung adenocarcinoma biomarkers based on bioinformatic analysis and human samples
Siyuan Dong (2020)
10.1093/nar/gkaa570
A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation
Estrella Guarino Almeida (2020)
10.3164/jcbn.19-80
Radiation-induced abnormal centrosome amplification and mitotic catastrophe in human cervical tumor HeLa cells and murine mammary tumor EMT6 cells
Masaki Fujimoto (2020)
10.1155/2020/8916729
AURKA Increase the Chemosensitivity of Colon Cancer Cells to Oxaliplatin by Inhibiting the TP53-Mediated DNA Damage Response Genes
B. Shan (2020)
See more
Semantic Scholar Logo Some data provided by SemanticScholar