Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Co-localization Of Corticotropin-releasing Factor And Vasopressin In Median Eminence Neurosecretory Vesicles

M. Whitnall, E. Mezey, H. Gainer
Published 1985 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Vasopressin (VP) potentiates the effect of corticotropin-releasing factor (CRF) on the secretion of adrenocorticotropic hormone (ACTH) from anterior pituitary cells in vitro1–3, and both CRF4–7 and VP6,8 have been found in portal blood. These data support the hypothesis that VP acts synergistically with CRF to cause the secretion of ACTH in vivo1–4,8,9 but the origin of the CRF and VP, and the physiology of their release, have not been precisely defined. Parvocellular cell bodies in the paraventricular nucleus (PVN) which project to the external zone of the median eminence can be stained for both CRF and VP after adrenalectomy10–12, and there is light microscopic imunocytochemical evidence that neurophysin (NP) may be located within some of the CRF-containing axons13. Electron microscopic immunocytochemical studies have demonstrated the presence of CRF14–16, VP17,18 and its ‘carrier’ protein, VP-associated neurophysin (NP-VP)17,18 in 100-nm neurosecretory vesicles (NSVs) in axons terminating near the portal capillary plexus in the external zone of the median eminence. If these peptides are extensively co-localized in the same NSVs in the median eminence, then coordinate secretion of CRF and VP in vivo is obligatory, at least in some physiological circumstances. We demonstrate in this report, using post-embedding electron microscopic immunocytochemistry on serial ultrathin sections, that CRF, VP and NP-VP are contained not only in the same axons and terminals, but in the same 100-nm NSVs in the median eminence of both normal and adrenalectomized rats. In addition, in the normal rat median eminence 44% of the CRF-positive axons and terminals stained strongly for VP and NP-VP, whereas in the adrenalectomized rat virtually all the CRF-positive structures in the median eminence showed strong staining for VP and NP-VP, indicating a transformation of one subpopulation of CRF-positive axons and terminals by adrenalectomy.
This paper references
10.1523/JNEUROSCI.05-01-00081.1985
Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies
Y. Ben-Barak (1985)
10.1111/j.1749-6632.1977.tb41872.x
VASOPRESSIN AND CORTICOTROPIN‐RELEASING FACTOR: AN AXONAL PATHWAY TO PORTAL CAPILLARIES IN THE ZONA EXTERNA OF THE MEDIAN EMINENCE CONTAINING VASOPRESSIN AND ITS INTERACTION WITH ADRENAL CORTICOIDS *
E. Zimmerman (1977)
10.1016/0196-9781(82)90037-7
In vitro potentiation of the activity of synthetic ovine corticotropin-releasing factor by arginine vasopressin
C. Turkelson (1982)
10.1210/ENDO-115-4-1639
Central modulation of immunoreactive corticotropin-releasing factor secretion by arginine vasopressin.
P. Plotsky (1984)
10.1210/ENDO-101-1-42
The effects of adrenalectomy and glucocorticoid replacement on vasopressin and vasopressin-neurophysin in the zona externa of the median eminence of the rat.
M. A. Stillman (1977)
10.1159/000123517
Comparative immunocytochemical localization of corticotropin releasing factor (CRF-41) and neurohypophysial peptides in the brain of Brattleboro and Long-Evans rats.
A. Burlet (1983)
10.1210/ENDO-111-4-1418
Presence of corticotropin releasing factor-like immunoreactivity in hypophysial portal blood.
Daniel M. Gibbs (1982)
10.1016/0006-8993(80)90791-X
[35S]cysteine labeled peptides transported to the neurohypophyses of adrenalectomized, lactating, and Brattleboro rats
J. Russell (1980)
10.1523/JNEUROSCI.05-01-00098.1985
Neurophysin in the hypothalamo-neurohypophysial system. II. Immunocytochemical studies of the ontogeny of oxytocinergic and vasopressinergic neurons
M. Whitnall (1985)
10.1038/315059A0
Co-localization of corticotropin releasing factor and vasopressin mRNA in neurones after adrenalectomy
B. Wolfson (1985)
10.1073/PNAS.81.6.1883
Co-expression of corticotropin-releasing factor and vasopressin immunoreactivity in parvocellular neurosecretory neurons of the adrenalectomized rat.
P. Sawchenko (1984)
10.1016/0006-8993(83)91189-7
Effect of the serotonin reuptake inhibitor fluoxetine on corticotropin-releasing factor and vasopressin secretion into hypophysial portal blood
Daniel M. Gibbs (1983)
10.1159/000123993
Comparative immunoelectron microscopic localization of corticotropin-releasing factor (CRF-41) and oxytocin in the rat median eminence.
F. Dreyfuss (1984)
10.1016/0196-9781(85)90047-6
Immunocytochemical identification of dynorphin-containing vesicles in brattleboro rats
M. Whitnall (1985)
10.1016/S0074-7696(08)62760-6
Neuronal secretory systems.
M. Castel (1984)
10.1038/299355A0
Corticotropin releasing activity of the new CRF is potentiated several times by vasopressin
G. Gillies (1982)
10.1523/JNEUROSCI.04-04-01118.1984
Corticotropin-releasing factor: co-expression within distinct subsets of oxytocin-, vasopressin-, and neurotensin-immunoreactive neurons in the hypothalamus of the male rat
P. Sawchenko (1984)
10.1210/ENDO-88-1-3
Potentiation by vasopressin of corticotropin release induced by corticotropin-releasing factor.
F. Yates (1971)
10.1002/AJA.1001470109
Electron microscopic immunohistochemical localization of vasopressin and neurophysin in the median eminence of normal and adrenalectomized rats.
D. Dubé (1976)
10.1073/PNAS.81.6.1854
Corticotropin-releasing factor-immunoreactive neurons of the paraventricular nucleus become vasopressin positive after adrenalectomy.
J. Kiss (1984)
10.1038/295299A0
Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin–neurophysin II precursor
H. Land (1982)
10.1159/000123973
Pituitary receptors for corticotropin-releasing factor: no effect of vasopressin on binding or activation of adenylate cyclase.
M. Holmes (1984)
10.1016/0167-0115(83)90011-3
Ultrastructural demonstration of ovine CRF-like immunoreactivity (oCRF-LI) in the rat hypothalamus: processes of magnocellular neurons establish membrane specializations with parvocellular neurons containing oCRF-LI
C. Léránth (1983)
10.1159/000123595
Ability of the CRF immunoreactive neurons of the paraventricular nucleus to produce a vasopressin-like material. Immunohistochemical demonstration in adrenalectomized guinea pigs and rats.
G. Tramu (1983)
10.1177/31.1.6187796
Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique.
M. Bendayan (1983)
10.1159/000123414
Immunoelectron microscopic localization of corticotropin-releasing factor in the rat hypothalamus.
G. Pelletier (1982)
10.1210/ENDO-113-3-1121
Effects of synthetic ovine corticotropin-releasing factor, glucocorticoids, catecholamines, neurohypophysial peptides, and other substances on cultured corticotropic cells.
W. Vale (1983)



This paper is referenced by
10.1007/978-1-4684-5131-3_17
Oxytocin and Vasopressin Secretion: New Perspectives
D. Lincoln (1986)
10.1016/0361-9230(88)90080-9
Dynamism of chemoarchitecture in the hypothalamic paraventricular nucleus
J. Kiss (1988)
Effects of Sex and Prenatal Stress on Vulnerability to Drugs.
M. B. Thomas (2012)
10.1016/0303-7207(87)90170-5
Evidence that AVP receptors in AtT-20 corticotrophs are not coupled to secretion of POMC-derived peptides
B. Lutz-Bucher (1987)
10.1007/978-81-322-2803-5_40
The Role of Vasopressin in Anxiety and Depression
J. C. Morales-Medina (2016)
10.1007/s00213-018-5099-x
Vasopressin and alcohol: a multifaceted relationship
Kathryn M Harper (2018)
10.1523/JNEUROSCI.19-13-05464.1999
Differential Regulation of Corticotropin-Releasing Hormone and Vasopressin Gene Transcription in the Hypothalamus by Norepinephrine
K. Itoi (1999)
10.1210/ENDO-126-4-1989
Vasopressin Mediates α1-Adrenergic Stimulation of Adrenocorticotropin Secretion*
S. Al-Damluji (1990)
10.2169/NAIKA.76.1856
A case of isolated adrenocorticotropic hormone (ACTH) deficiency associated with severe hyponatremia and an empty sella
淳 中川 (1987)
10.1007/978-1-4615-8129-1_16
Aging of Rodent Vasopressin Systems
P. Aravich (1987)
10.1078/0171-9335-00163
Differential sorting of constitutively co-secreted proteins in the ovarian follicle cells of Drosophila.
I. Trougakos (2001)
10.1016/S0079-6123(08)64586-0
The role of corticotropin-releasing hormone in the pathogenesis of Cushing's disease, anorexia nervosa, alcoholism, affective disorders and dementia.
F. Holsboer (1992)
10.1016/0301-0082(93)90035-Q
Regulation of the hypothalamic corticotropin-releasing hormone neurosecretory system
M. Whitnall (1993)
10.1210/EDRV-10-2-182
Maturation and activation of hypothalamic-pituitary adrenal function in fetal sheep.
J. Challis (1989)
10.1002/AJA.1001750211
Postembedding immunogold labeling for electron microscopy using "LR White" resin.
B. Timms (1986)
10.1002/CNE.903520403
Physiological regulation of peptide messenger RNA colocalization in rat hypothalamic paraventricular medial parvicellular neurons
A. Watts (1995)
10.1038/s41598-019-44776-1
Viral rescue of magnocellular vasopressin cells in adolescent Brattleboro rats ameliorates diabetes insipidus, but not the hypoaroused phenotype
K. Schatz (2019)
10.1016/j.alcohol.2015.04.004
Divergent regulation of distinct glucocorticoid systems in alcohol dependence.
Scott Edwards (2015)
10.1210/ENDO-120-5-2180
Vasopressin-containing and vasopressin-deficient subpopulations of corticotropin-releasing factor axons are differentially affected by adrenalectomy.
M. Whitnall (1987)
10.1007/978-1-4471-0931-0_2
Signals from the hypothalamus to the pituitary during chronic immune responses
M. Harbuz (1997)
10.1267/AHC.21.231
APPLICATION OF IMMUNOHISTOCHEMISTRY IN THE STUDY OF FUNCTIONAL MORPHOLOGY
S. Daikoku (1988)
10.1016/J.MOLBRAINRES.2005.03.016
Pituitary adenylate cyclase-activating polypeptide (PACAP) mimics neuroendocrine and behavioral manifestations of stress: Evidence for PKA-mediated expression of the corticotropin-releasing hormone (CRH) gene.
A. Agarwal (2005)
10.1111/j.1749-6632.1987.tb24948.x
The CRH Motoneuron: Differential Peptide Regulation in Neurons with Possible Synaptic, Paracrine, and Endocrine Outputs
L. Swanson (1987)
10.1210/ENDO-123-2-713
The effects of cortisol, vasopressin (AVP), and corticotropin-releasing factor administration on pulsatile adrenocorticotropin, alpha-melanocyte-stimulating hormone, and AVP secretion in the pituitary venous effluent of the horse.
J. Livesey (1988)
10.1007/978-1-4612-3954-3_8
ACTH: Normal Physiology
R. Dorin (1997)
10.1159/000082876
Stress-Induced Sensitization of the Hypothalamic-Pituitary Adrenal Axis Is Associated with Alterations of Hypothalamic and Pituitary Gene Expression
K. O'connor (2004)
10.1016/0197-4580(94)00159-6
Hypothalamic-pituitary-adrenal system function in patients with alzheimer's disease
M. Hatzinger (1995)
10.1111/j.1749-6632.1997.tb48393.x
The Hypothalamic‐Pituitary‐Adrenal Axis in Autoimmunity
M. Harbuz (1997)
10.1002/CNE.902750103
Distributions of pro‐vasopressin expressing and pro‐vasopressin deficient CRH neurons in the paraventricular hypothalamic nucleus of colchicine‐treated normal and adrenalectomized rats
M. Whitnall (1988)
10.1002/JNR.490190404
Demonstration of glucocorticoid receptor‐like immunoreactivity in glucocorticoid‐sensitive vasopressin and corticotropin‐releasing factor neurons in the hypothalamic paraventricular nucleus
R. M. Uth (1988)
10.3389/fendo.2016.00137
Paraventricular Hypothalamic Mechanisms of Chronic Stress Adaptation
J. Herman (2016)
10.1210/ENDO-125-4-1921
Kinetic actions and interactions of arginine vasopressin, angiotensin-II, and oxytocin on adrenocorticotropin secretion by rat anterior pituitary cells in the microperifusion system.
T. Watanabe (1989)
See more
Semantic Scholar Logo Some data provided by SemanticScholar