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Chimaeras Of Myc Oncoprotein And Steroid Receptors Cause Hormone-dependent Transformation Of Cells

M. Eilers, D. Picard, K. R. Yamamoto, J. Bishop
Published 1989 · Biology, Medicine

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THE human proto-oncogene myc encodes a nuclear phosphoprotein whose primary biochemical function is still unknown1,2. To facilitate further study of that function, we have created conditional alleles of myc by fusing the hormone-binding domain of the human oestrogen receptor gene to the 5' or the 3' end of human myc. The two chimaeric genes, designated mycer and ermyc, encode proteins that bind oestrogen with high affinity. Expression of one of the genes, mycer, transforms a rat fibroblast cell line in a tightly oestrogen-dependent manner. Transformation is dependent on the presence of a functional myc gene in the chimaera and is reversible upon removal of the hormone. The chimaeric genes will be useful tools to study the mechanisms by which Myc affects cellular phenotype. Recently, chimaeras between the adenovirus El A protein and the hormone binding domain of the rat glucocorticoid receptor were shown to activate transcription in a manner characteristic for El A, but in a hormone regulated manner10. We there-fore asked whether the same strategy could be applied to the product of myc.
This paper references

This paper is referenced by
Molecular genetics of acute promyelocytic leukemia: the t(15,17) translocation
H. Thé (2009)
Hormone-induced apoptosis by Fas-nuclear receptor fusion proteins: novel biological tools for controlling apoptosis in vivo.
H. Takebayashi (1996)
Inhibition of the Raf-1 kinase by cyclic AMP agonists causes apoptosis of v-abl-transformed cells.
E. Weissinger (1997)
Apoptosis and a re-investigation of the biologic basis for cancer therapy.
A. D'Amico (1994)
Steroid-binding domains for regulating the functions of heterologous proteins in cis.
D. Picard (1993)
Plant transcription factors: present knowledge and future challenges.
F. Katagiri (1992)
myc family oncogenes in the development of normal and neoplastic cells.
R. Depinho (1991)
The Myc Gene
L. Soucek (2013)
Identification of a large Myc-binding protein that contains RCC1-like repeats.
Q. Guo (1998)
LIF/STAT3 controls ES cell self-renewal and pluripotency by a Myc-dependent mechanism
P. Cartwright (2005)
Induction of TNF‐sensitive cellular phenotype by c‐Myc involves p53 and impaired NF‐κB activation
J. Klefstrom (1997)
Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis
Jean-Paul Herman (2012)
A yeast sensor of ligand binding
C. Tucker (2001)
ID2 expression is not associated with MYCN amplification or expression in human neuroblastomas.
Q. Wang (2003)
c-Myc-induced genomic instability.
S. Mai (2003)
Targeting c-Myc as a novel approach for hepatocellular carcinoma.
Che-Pin Lin (2010)
N-Myc shares cellular functions wiht c-Myc.
S. Aubry (2000)
Cdc25 cell-cycle phosphatase as a target of c-myc
K. Galaktionov (1996)
Deregulation of Scribble Promotes Mammary Tumorigenesis and Reveals a Role for Cell Polarity in Carcinoma
L. Zhan (2008)
Chemically Regulated Zinc Finger Transcription Factors*
R. Beerli (2000)
Transcriptional activation by Nur77, a growth factor-inducible member of the steroid hormone receptor superfamily.
I. Davis (1991)
Activation of an inducible c-FosER fusion protein causes loss of epithelial polarity and triggers epithelial-fibroblastoid cell conversion
E. Reichmann (1992)
Cell size regulation by the human TSC tumor suppressor proteins depends on PI3K and FKBP38
M. Rosner (2003)
Regulation of MKL1 via actin cytoskeleton dynamics drives adipocyte differentiation.
H. Nobusue (2014)
Modular structure of transcription factors: Implications for gene regulation
A. Frankel (1991)
Characterization of the Minimalist Hybrid Protein Inhibitor ME47 as a Potential Anti-tumour Agent Designed to Target Myc Activity in Cancer
L. Lustig (2013)
Steroid Hormones and Hormone Receptors in Neoplastic Diseases
C. Distelhorst (1991)
Inhibitory effects of emodin on the proliferation of cultured rat vascular smooth muscle cell‐induced by angiotensin II
S. Wang (2008)
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C. Lavau (2006)
Myc: a single gene controls both proliferation and apoptosis in mammalian cells
L. Desbarats (2005)
An integrated database of genes responsive to the Myc oncogenic transcription factor: identification of direct genomic targets
K. Zeller (2003)
Protein and Nucleotide Biosynthesis Are Coupled by a Single Rate-Limiting Enzyme, PRPS2, to Drive Cancer
J. T. Cunningham (2014)
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