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Muscle Progenitor Cells Failing To Respond To Positional Cues Adopt Non-myogenic Fates In Myf-5 Null Mice

S. Tajbakhsh, D. Rocancourt, M. Buckingham
Published 1996 · Biology, Medicine

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MICE that have mutations in both myogenic transcription factors Myf-5 and MyoD totally lack skeletal muscle fibres and their precursor myoblasts1, whereas with either mutation alone, muscle is present2,3. Skeletal muscle in the vertebrate body is derived from epithelial somites that respond to environmental signals to form the dorsal epithelial dermomyotome (dermis, muscle) and ventral mesenchymal sclerotome (axial skeleton, ribs) 4,5. The first muscle, the myotome, forms centrally in the somite, when only myf-5 is programming myogenesis. By targeting the nlacZ reporter gene into the myf-5 locus, we demonstrate that β-galactosidase muscle progenitor cells are present in the dermomyotome of myf-5 null embryos, and that they undergo a normal epithelial-mesenchymal transition; however, they migrate aberrantly. Dorsally, they accumulate under the ectoderm and express a non-muscle dermal marker, Dermo-1. Ventrally, β-galactosidase+ cells also fail to localize correctly, express a cartilage marker scleraxis, and are subsequently found in ribs. Therefore Myf-5 protein is necessary for cells to respond correctly to positional cues in the embryo and to adopt their myogenic fate. In its absence, muscle progenitors, having activated myf-5, remain multipotent and differentiate into other somitic derivatives according to their local environment.
This paper references
Initial steps of myogenesis in somites are independent of influence from axial structures.
E. Bober (1994)
10.1016/0955-0674(93)90088-8
Extracellular matrix alters epithelial differentiation.
E. Hay (1993)
10.1006/DBIO.1995.0023
Dermo-1: a novel twist-related bHLH protein expressed in the developing dermis.
L. Li (1995)
10.1002/(SICI)1097-0177(199607)206:3<291::AID-AJA6>3.0.CO;2-D
Gene targeting the myf‐5 locus with nlacZ reveals expression of this myogenic factor in mature skeletal muscle fibres as well as early embryonic muscle
S. Tajbakhsh (1996)
Alterations in somite patterning of Myf-5-deficient mice: a possible role for FGF-4 and FGF-6.
S. Grass (1996)
10.1016/0092-8674(93)90621-V
MyoD or Myf-5 is required for the formation of skeletal muscle
M. Rudnicki (1993)
10.1073/PNAS.91.2.747
Mouse limb muscle is determined in the absence of the earliest myogenic factor myf-5.
S. Tajbakhsh (1994)
10.1073/PNAS.87.24.9918
Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection.
T. Yagi (1990)
10.1007/BF00304424
Early stages of chick somite development
B. Christ (2004)
10.1073/PNAS.93.9.4213
Pax3 modulates expression of the c-Met receptor during limb muscle development.
J. Epstein (1996)
10.1073/PNAS.92.6.2254
Myoblast differentiation during mammalian somitogenesis is dependent upon a community effect.
G. Cossu (1995)
10.1006/DBIO.1994.1157
Neural crest cells prefer the myotome's basal lamina over the sclerotome as a substratum.
Kathryn W. Tosney (1994)
10.1016/S0168-9525(00)88994-5
In situ hybridization and beta-galactosidase: a powerful combination for analysing transgenic mice.
S. Tajbakhsh (1995)
Early expression of the myogenic regulatory gene, myf-5, in precursor cells of skeletal muscle in the mouse embryo.
M. Ott (1991)
Scleraxis: a basic helix-loop-helix protein that prefigures skeletal formation during mouse embryogenesis.
P. Cserjesi (1995)
10.1016/0168-9525(96)10025-1
How is myogenesis initiated in the embryo?
G. Cossu (1996)
Lineage restriction of the myogenic conversion factor myf-5 in the brain.
S. Tajbakhsh (1995)
The neural tube/notochord complex is necessary for vertebral but not limb and body wall striated muscle differentiation.
P. M. Rong (1992)
Notochord signals control the transcriptional cascade of myogenic bHLH genes in somites of quail embryos.
M. Pownall (1996)
10.1002/BIES.950160607
Drosophila wingless: A paradigm for the function and mechanism of Wnt signaling
E. Siegfried (1994)
Expression of the met receptor tyrosine kinase in muscle progenitor cells in somites and limbs is absent in Splotch mice.
X. Yang (1996)
10.1038/336772A0
A community effect in animal development
J. Gurdon (1988)
10.1016/0092-8674(92)90508-A
Inactivation of MyoD in mice leads to up-regulation of the myogenic HLH gene Myf-5 and results in apparently normal muscle development
M. Rudnicki (1992)
10.1016/0168-9525(92)90373-C
Making muscle in mammals.
M. Buckingham (1992)
10.1093/NAR/20.14.3795
A new mouse embryonic stem cell line with good germ line contribution and gene targeting frequency.
T. Magin (1992)
10.1016/0092-8674(92)90507-9
Targeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death
T. Braun (1992)
Single cell analysis of mesoderm formation in the Xenopus embryo.
S. Godsave (1991)



This paper is referenced by
MyoD and Myf-5 differentially regulate the development of limb versus trunk skeletal muscle.
B. Kablar (1997)
10.1006/EXCR.2002.5653
Kinetics of myoblast proliferation show that resident satellite cells are competent to fully regenerate skeletal muscle fibers.
P. Zammit (2002)
Functional analysis of insulin-like growth factor binding protein -4 and -6 in transgenic mice
R. Zhou (2003)
10.1101/sqb.2008.73.006
Regulation of skeletal muscle stem cell behavior by Pax3 and Pax7.
M. Lagha (2008)
Régulation de la différenciation du muscle strié squelettique par la voie let-7 – E2F5
J. Kropp (2014)
10.1016/S0072-9752(07)86018-0
Muscle diseases and aging.
P. Serdaroğlu (2007)
10.20381/RUOR-457
Regulation of Satellite Cell Homeostasis by C/EBPβ: Therapeutic Perspectives
N. Lala-Tabbert (2016)
10.1242/dev.00557
Myf5 expression in somites and limb buds of mouse embryos is controlled by two distinct distal enhancer activities
A. Buchberger (2003)
10.1101/GAD.382806
A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb.
L. Bajard (2006)
10.1016/j.ydbio.2009.05.554
Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD.
Onur Kanisicak (2009)
Regulatory Factors that Reveal Three Distinct Adipocytes : The Brown, the White and the Brite
Tomas B. Waldén (2010)
Ccndbp 1 is a novel positive regulator of skeletal myogenesis
Y. Huang (2016)
10.1007/PL00008244
cDermo-1 expression indicates a role in avian skin development
M. Scaal (2001)
10.1016/j.devcel.2015.07.003
A Cranial Mesoderm Origin for Esophagus Striated Muscles.
Swetha Gopalakrishnan (2015)
10.1016/S0070-2153(08)60244-6
Drosophila myogenesis and insights into the role of nautilus.
S. Abmayr (1998)
10.1242/dev.053421
Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis
A. L'honoré (2010)
10.1046/J.1432-0436.2001.680412.X
Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation.
A. Asakura (2001)
10.1096/fj.05-5125fje
Transcriptional profiling of myostatin‐knockout mice implicates Wnt signaling in postnatal skeletal muscle growth and hypertrophy
Carissa A Steelman (2006)
10.1007/s00018-006-6015-9
Skeletal muscle progenitor cells: from embryo to adult
F. Relaix (2006)
10.1016/J.YEXCR.2006.04.012
Myostatin regulation of muscle development: molecular basis, natural mutations, physiopathological aspects.
Joulia-Ekaza Dominique (2006)
10.1097/NEN.0b013e3182084391
Human Neurotrophin Receptor p75NTR Defines Differentiation-Oriented Skeletal Muscle Precursor Cells: Implications for Muscle Regeneration
E. Colombo (2011)
10.1002/dvdy.20589
Presence of neurotrophic factors in skeletal muscle correlates with survival of spinal cord motor neurons
B. Kablar (2005)
Satellite Cell Subpopulations and Environmental Mediators of their Function: Implications for Stem Cell Therapy in Skeletal Muscle
A. Neal (2013)
10.1016/S0925-4773(98)00036-7
The generation and interpretation of positional information within the vertebrate myotome
P. Currie (1998)
10.1016/B978-012505255-9/50042-0
Myogenic Cell Specification during Somitogenesis
M. Buckingham (1999)
10.1369/jhc.2010.956730
Efficient Single Muscle Fiber Isolation from Alcohol-Fixed Adult Muscle following β-Galactosidase Staining for Satellite Cell Detection
Mayank Verma (2011)
In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse skeletal muscle.
R. N. Cooper (1999)
10.1091/MBC.9.7.1891
RhoA GTPase and serum response factor control selectively the expression of MyoD without affecting Myf5 in mouse myoblasts.
G. Carnac (1998)
10.1016/J.YDBIO.2007.06.006
Cellular heterogeneity during vertebrate skeletal muscle development.
S. Biressi (2007)
10.1016/j.ydbio.2010.04.024
Hox genes and regional patterning of the vertebrate body plan.
M. Mallo (2010)
Evaluation of the microsatellite polymorphism in intron I of the myostatin gene (MSTN) in Latvian Blue cattle breed.
J. Mazvērsīte (2008)
10.1002/(SICI)1097-0177(199911)216:3<219::AID-DVDY1>3.0.CO;2-J
Early development of the myotome in the mouse
S. Venters (1999)
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