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Enhanced Growth Inhibition Of Metastatic Lung Tumors By Intravenous Injection Of ATRA-cationic Liposome/IL-12 PDNA Complexes In Mice

P. Charoensit, S. Kawakami, Y. Higuchi, F. Yamashita, M. Hashida
Published 2010 · Chemistry, Medicine

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Interleukin 12 (IL-12) is a proinflammatory cytokine with antitumor activity. All-trans–retinoic acid (ATRA) exerts antitumor effects by regulating a variety of gene expressions, including tumor necrosis factor receptor 1 (TNFR1), increases the number of TNFR1 and potentiates TNF-α-induced apoptosis in cancer cells. In this study, ATRA-incorporated cationic liposome (ATRA-cationic liposome)/IL-12 plasmid DNA (pDNA) complexes were prepared to improve therapeutic efficacy of cationic liposome/IL-12 pDNA complexes in a mouse model of metastatic lung tumor after intravenous injection. IL-12 production in lungs by ATRA-cationic liposome/IL-12 pDNA complexes was comparable with that by cationic liposome/IL-12 pDNA complexes. The number of metastatic tumor cells (colon26/Luc) was quantitatively evaluated by measuring luciferase activity. ATRA-cationic liposome/IL-12 pDNA complexes reduced the number of colon26/Luc cells and tumor nodules in lungs. ATRA-cationic liposome/IL-12 pDNA complexes significantly prolonged the survival time of mice, whereas cationic liposome/IL-12 pDNA only slightly prolonged it. ATRA-cationic liposome/IL-12 pDNA complexes increased the TNFR1 mRNA upregulation and the number of apoptotic cells in the lung. Moreover, reduced serum alanine transaminase (ALT) and aspartate transaminase (AST) activities were observed in mice treated with ATRA-cationic liposome/IL-12 pDNA complexes. These results suggest that intravenous injection of ATRA-cationic liposome/IL-12 pDNA complexes is an effective method for the treatment of lung metastasis in mice.
This paper references
The role of tissue macrophages in the induction of proinflammatory cytokine production following intravenous injection of lipoplexes
F. Sakurai (2002)
Intravenous cytokine gene delivery by lipid-DNA complexes controls the growth of established lung metastases.
S. Dow (1999)
Phase 1/2 Clinical Trial of Interferon α2b and Weekly Liposome-encapsulated All-trans Retinoic Acid in Patients With Advanced Renal Cell Carcinoma
S. Boorjian (2007)
Interleukin-12 in anti-tumor immunity and immunotherapy.
M. Colombo (2002)
Doxorubicin Directs the Accumulation of Interleukin-12–Induced IFNγ into Tumors for Enhancing STAT1–Dependent Antitumor Effect
Shiguo Zhu (2007)
All-trans-retinoic acid upregulates TNF receptors and potentiates TNF-induced activation of nuclear factors-κB, activated protein-1 and apoptosis in human lung cancer cells
S. Manna (2000)
Preclinical Toxicity, Toxicokinetics, and Antitumoral Efficacy Studies of DTS-201, a Tumor-Selective Peptidic Prodrug of Doxorubicin
D. Ravel (2008)
Evaluation of proinflammatory cytokine production and liver injury induced by plasmid DNA/cationic liposome complexes with various mixing ratios in mice.
Y. Ito (2009)
Effect of retinoids on LPS-induced COX-2 expression and COX-2 associated PGE(2) release from mouse peritoneal macrophages and TNF-alpha release from rat peripheral blood mononuclear cells.
B. Kim (2004)
Synergistic stimulation of MHC class I and IRF‐1 gene expression by IFN‐γ and TNF‐α in oligodendrocytes
C. Agresti (1998)
Retinoic acid receptors and cancers.
D. Soprano (2004)
The Toxicology of Interleukin-12: A Review
B. Car (1999)
Surface characterization of biomaterials: B.D. Ratner (Ed.), Elsevier, Amsterdam, 1988, 344 pages, Dfl. 230.00, $121.05
G. Llanos (1989)
Differentiation therapy.
R. Lotan (1990)
Inhibition of Metastatic Tumor Growth in Mouse Lung by Repeated Administration of Polyethylene Glycol-Conjugated Catalase
K. Hyoudou (2004)
Phase 1/2 clinical trial of interferon alpha2b and weekly liposome-encapsulated all-trans retinoic acid in patients with advanced renal cell carcinoma.
S. Boorjian (2007)
All‐trans retinoic acid suppresses liver injury induced by Propionibacterium acnes and lipopolysaccharide in rats
K. Motomura (1997)
Role of interferon-gamma in interleukin 12-induced pathology in mice.
B. Car (1995)
Blast crisis accompanied with occurrence of the point-mutational activation of N-ras proto-oncogene and the chromosomal abnormality inv(3q) in chronic myelogenous leukemia presenting thrombocytosis.
M. Okabe (1990)
Management of acute promyelocytic leukemia: recommendations from an expert panel on behalf of the European LeukemiaNet.
M. Sanz (2009)
Tumour necrosis factor receptor I (p55) is upregulated on endothelial cells by exposure to the tumour-derived cytokine endothelial monocyte- activating polypeptide II (EMAP-II).
A. Berger (2000)
Evaluation of Proinflammatory Cytokine Production Induced by Linear and Branched Polyethylenimine/Plasmid DNA Complexes in Mice
S. Kawakami (2006)
Retinoic acids inhibit inducible nitric oxide synthase expression in mesangial cells.
P. Datta (1999)
Retinoic acid
Gonçalo C. Vilhais-Neto (2008)
The induction of differentiation in teratocarcinoma stem cells by retinoic acid
Sidney Strickland (1978)
Induction of apoptosis in A549 human lung cancer cells by all-trans retinoic acid incorporated in DOTAP/cholesterol liposomes.
S. Kawakami (2006)
Pharmacokinetics, tissue distribution, and expression efficiency of plasmid [33P]DNA following intravenous administration of DNA/cationic lipid complexes in mice: use of a novel radionuclide approach.
G. Osaka (1996)
All-trans-retinoic acid improves differentiation of myeloid cells and immune response in cancer patients.
N. Mirza (2006)
Inhibition of metastatic tumor growth by targeted delivery of antioxidant enzymes.
M. Nishikawa (2005)
Is cytarabine useful in the treatment of acute promyelocytic leukemia? Results of a randomized trial from the European Acute Promyelocytic Leukemia Group.
L. Adès (2006)
Incorporation of all‐trans retinoic acid into lipoplexes inhibits nuclear factor κB activation mediated liver injury induced by lipoplexes in mice
P. Charoensit (2008)
Tumor Necrosis Factor-α–Induced Apoptosis in Murine Cytomegalovirus Retinitis
J. Zhou (2007)
Inhibition of pulmonary metastasis in mice by all-trans retinoic acid incorporated in cationic liposomes.
Sachiko Suzuki (2006)
Suppressive effects of all-trans retinoic acid on the lipopolysaccharide-stimulated release of tumor necrosis factor-α and nitric oxide by rat Kupffer cells in vitro
K. Motomura (1996)
Inhibition of peritoneal dissemination of tumor cells by single dosing of phosphodiester CpG oligonucleotide/cationic liposome complex.
Y. Kuramoto (2006)
Combination of local, non-viral IL12 gene therapy and systemic paclitaxel chemotherapy in a syngeneic ID8 mouse model for human ovarian cancer.
M. Janát-Amsbury (2006)
Safety and clinical activity of the combination of 5-azacytidine, valproic acid, and all-trans retinoic acid in acute myeloid leukemia and myelodysplastic syndrome.
A. Soriano (2007)
Differentiation syndrome in patients with acute promyelocytic leukemia treated with all-trans retinoic acid and anthracycline chemotherapy: characteristics, outcome, and prognostic factors.
P. Montesinos (2009)
Management of acute promyelocytic leukemia
M. Tallman (2002)
Physicochemical and pharmacokinetic characteristics of plasmid DNA/cationic liposome complexes.
R. Mahato (1995)
Taxol: a novel investigational antimicrotubule agent.
E. Rowinsky (1990)
Contribution of plasmid DNA to hepatotoxicity after systemic administration of lipoplexes.
S. Loisel (2001)
Retinoids in cancer therapy and chemoprevention: promise meets resistance
S. Freemantle (2003)
Therapeutic effect of all-trans retinoic acid on rats with anti-GBM antibody glomerulonephritis.
Susumu Oseto (2003)
Management of acute promyelocytic
MS Tallman (1990)
N-acetylcysteine attenuates lipopolysaccharide-induced apoptotic liver damage in D-galactosamine-sensitized mice.
H. Wang (2007)
CpG motifs in bacterial DNA trigger direct B-cell activation
Arthur M. Krieg (1995)

This paper is referenced by
Strategies on the nuclear-targeted delivery of genes
Jing Yao (2013)
Gene therapy for cardiovascular disease mediated by ultrasound and microbubbles
Zhiyi Chen (2013)
Liposomal delivery of proteins and peptides
Janani Swaminathan (2012)
All-trans retinoic acid-incorporated nanoparticles of deoxycholic acid-conjugated dextran for treatment of CT 26 colorectal carcinoma cells
Y. I. Jeong (2013)
Retinoic Acid Receptor α Mediates All-trans-retinoic Acid-induced Klf4 Gene Expression by Regulating Klf4 Promoter Activity in Vascular Smooth Muscle Cells*
J. Shi (2012)
A WKYMVm-Containing Combination Elicits Potent Anti-Tumor Activity in Heterotopic Cancer Animal Model
S. D. Kim (2012)
Application of cationic liposomes for delivery of nucleic acids
G. Shim (2013)
A Novel Self-Assembled Mitochondria-Targeting Protein Nanoparticle Acting as Theranostic Platform for Cancer.
Xin-Jie Zhu (2019)
Effect of amide linkage of PEG-lipid conjugates on the stability and cytotoxic activity of goniodiol loaded in PEGylated liposomes
P. Charoensit (2019)
Supramolecular hydrogels for long-term bioengineered stem cell therapy.
Junseok Yeom (2015)
Non-viral Delivery of Nucleic Acids: Insight Into Mechanisms of Overcoming Intracellular Barriers
M. Durymanov (2018)
Interleukin 12: Stumbling Blocks and Stepping Stones to Effective Anti-Tumor Therapy
H. Pegram (2012)
Advancements in Tumor Immunotherapy and Cancer Vaccines
H. Arnouk (2012)
Localized Interleukin-12 for Cancer Immunotherapy
K. G. Nguyen (2020)
Hepatic Vitamin A Preloading Reduces Colorectal Cancer Metastatic Multiplicity in a Mouse Xenograft Model
E. Park (2012)
Polynucléotides codant pour l'interleukine 12 (il-12) et leurs utilisations
Joshua P. Frederick (2017)
Liposome-targeted delivery for highly potent drugs
Hassan Bardania (2017)
Glycosylation-mediated targeting of carriers.
S. Kawakami (2014)
Interleukin 12: still a promising candidate for tumor immunotherapy?
W. Lasek (2014)
Interleukin 12: Antitumor Activity and Immunotherapeutic Potential in Oncology
W. Lasek (2016)
Emerging biotechnological strategies for non-viral antiangiogenic gene therapy
C. Liu (2012)
Anti-tumor activity of all-trans retinoic acid-incorporated glycol chitosan nanoparticles against HuCC-T1 human cholangiocarcinoma cells.
Kyu-Don Chung (2012)
All-trans retinoic acid-incorporated nanoparticles of deoxycholic acid-conjugated dextran for treatment of CT26 colorectal carcinoma cells
Y. I. Jeong (2013)
Therapeutic Efficacy and Systemic Antitumor T Cell Immunity Induced by RheoSwitch-Regulated IL-12 Expression after Intratumoral Injection of Adenovirus Vector or Vector-Transduced Dendritic Cells
T. Chan (2014)
Nucleic acid delivery : biocompatible yet efficient platforms
M. I. Carlos (2015)
Antitumor Effects of IL-12 in Preclinical Studies
W. Lasek (2016)
HDAC2 phosphorylation-dependent Klf5 deacetylation and RARα acetylation induced by RAR agonist switch the transcription regulatory programs of p21 in VSMCs
B. Zheng (2011)
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