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Labeling Of Immune Cells For In Vivo Imaging Using Magnetofluorescent Nanoparticles
M. Pittet, F. Swirski, Frederick D Reynolds, L. Josephson, R. Weissleder
Published 2006 · Medicine, Biology
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Observation of immune and stem cells in their native microenvironments requires the development of imaging agents to allow their in vivo tracking. We describe here the synthesis of magnetofluorescent nanoparticles for cell labeling in vitro and for multimodality imaging of administered cells in vivo. MION-47, a prototype monocrystalline iron oxide nanoparticle, was first converted to an intermediate bearing a fluorochrome and amine groups, then reacted with either HIV-Tat peptide or protamine to yield a nanoparticle with membrane-translocating properties. We describe how to assess optimal cell labeling with tests of cell phenotype and function. Synthesis of magnetofluorescent nanoparticles and cell-labeling optimization can be realized in 48 h, whereas nanoparticle uptakes and retention studies may generally take up to 120 h. Labeled cells can be detected by magnetic resonance imaging, fluorescence reflectance imaging, fluorescence-mediated tomography, confocal microscopy and flow cytometry, and can be purified based on their fluorescent or magnetic properties. The present protocol focuses on T-cell labeling but can be used for labeling a variety of circulating cells.
This paper references
Transfection agent induced nanoparticle cell
K. Montet-Abou (2005)
Serial in vivo imaging of the targeted migration of human HSV-TK-transduced antigen-specific lymphocytes
G. Koehne (2003)
In Vivo Tracking of Stem Cells for Clinical Trials in Cardiovascular Disease
J. V. Frangioni (2004)
Ex Vivo IFN-γ Secretion by Circulating CD8 T Lymphocytes: Implications of a Novel Approach for T Cell Monitoring in Infectious and Malignant Diseases1
M. Pittet (2001)
MYC inactivation uncovers pluripotent differentiation and tumour dormancy in hepatocellular cancer
Catherine M. Shachaf (2004)
In vivo high resolution three-dimensional imaging of antigen-specific cytotoxic T-lymphocyte trafficking to tumors.
M. Kircher (2003)
Shedding light onto live molecular targets
R. Weissleder (2003)
Visualization of a primary anti-tumor immune response by positron emission tomography.
C. Shu (2005)
Ex Vivo Characterization of Allo-MHC-Restricted T Cells Specific for a Single MHC-Peptide Complex1
M. Pittet (2006)
Cell-specific targeting of nanoparticles by multivalent attachment of small molecules
R. Weissleder (2005)
Transfection Agent Induced Nanoparticle Cell Loading
Karin Montet-Abou (2005)
Scaling down imaging: molecular mapping of cancer in mice
R. Weissleder (2002)
Tat-mediated delivery of heterologous proteins into cells.
S. Fawell (1994)
Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells
M. Lewin (2000)
In vivo protein transduction: delivery of a biologically active protein into the mouse.
S. Schwarze (1999)
implications of a novel approach for T cell monitoring in infectious and malignant diseases
Looking and listening to light: the evolution of whole-body photonic imaging
V. Ntziachristos (2005)
T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases
T. Mempel (2004)
Expansion and functional maturation of human tumor antigen-specific CD8+ T cells after vaccination with antigenic peptide.
M. Pittet (2001)
Synergistic Antitumor Effects of Immune Cell-Viral Biotherapy
S. Thorne (2006)
TAT-p27Kip1 induces cell migration
Murine B16 Melanomas Expressing High Levels of the Chemokine Stromal-Derived Factor-1/CXCL12 Induce Tumor-Specific T Cell Chemorepulsion and Escape from Immune Control1
F. Vianello (2006)
Protamine as an efficient membrane-translocating peptide.
F. Reynolds (2005)
Magnetic resonance tracking of dendritic cells in melanoma patients for monitoring of cellular therapy
I. J. Vries (2005)
Immune Evasion by Murine Melanoma Mediated through CC Chemokine Receptor-10
T. Murakami (2003)
Adoptive-cell-transfer therapy for the treatment of patients with cancer
M. Dudley (2003)
Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration
H. Nagahara (1998)
Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI.
A. Arbab (2004)
Cancer Regression and Autoimmunity in Patients After Clonal Repopulation with Antitumor Lymphocytes
M. Dudley (2002)
In vivo tracking of tumor-specific T cells.
C. Yee (2001)
Regulatory T cells suppress tumor-specific CD8 T cell cytotoxicity through TGF-β signals in vivo
M. Chen (2005)
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In vivo imaging in cancer.
J. Condeelis (2010)
Modulation of T2 relaxation time by light-induced, reversible aggregation of magnetic nanoparticles.
E. Osborne (2010)
Recyclable and stable silver deposited magnetic nanoparticles with poly (vinyl pyrrolidone)-catechol coated iron oxide for antimicrobial activity.
Tamim Mosaiab (2013)
Theranostic Magnetic Nanostructures (MNS) for Cancer.
V. Nandwana (2015)
Intracellular transduction using cell-penetrating peptides.
R. Sawant (2010)
Synthesis and high performance of magnetofluorescent polyelectrolyte nanocomposites as MR/near-infrared multimodal cellular imaging nanoprobes.
H. Kim (2011)
Stabilization and Characterization of Iron Oxide Superparamagnetic Core-Shell Nanoparticles for Biomedical Applications
E. Reimhult (2014)
Cell-targeting and cell-penetrating peptides for delivery of therapeutic and imaging agents.
R. Juliano (2009)
Development of Antibody-Tagged Nanoparticles for Detection of Transplant Rejection Using Biomagnetic Sensors
K. Butler (2013)
A screening paradigm for the design of improved polymer-coated superparamagnetic iron oxide nanoparticles
Shu Han Chen (2009)
Uptake and Intracellular Fate of Peptide Surface‐Functionalized Silica Hybrid Magnetic Nanoparticles In Vitro
R. Digigow (2015)
Covalent assembly of nanoparticles as a peptidase-degradable platform for molecular MRI
F. Pérez-Balderas (2017)
Dual imaging and photoactivated nanoprobe for controlled cell tracking.
Sarit S Agasti (2013)
IRM cellulaire de lymphocytes marqués par des nanoparticules d'oxydes de fer : application au diagnostic en cancérologie
P. Mowat (2007)
Imaging in the era of molecular oncology
R. Weissleder (2008)
Efficient γ-amino-proline-derived cell penetrating peptide-superparamagnetic iron oxide nanoparticle conjugates via aniline-catalyzed oxime chemistry as bimodal imaging nanoagents.
S. Cavalli (2012)
Towards polymetallic lanthanide complexes as dual contrast agents for magnetic resonance and optical imaging.
E. Debroye (2014)
Optimization of molecularly targeted MRI in the brain: empirical comparison of sequences and particles
N. Zarghami (2018)
In situ synthesis of Eu(Tp)3 complex inside the pores of mesoporous silica nanoparticles
Chandrashekhar Malba (2013)
Biomedical Applications of Nanoparticles
G. L. Prasad (2009)
Toxicology of wear particles of cobalt-chromium alloy metal-on-metal hip implants Part II: Importance of physicochemical properties and dose in animal and in vitro studies as a basis for risk assessment.
A. Madl (2015)
In vivo and ex vivo applications of gold nanoparticles for biomedical SERS imagingi.
M. V. Yigit (2012)
Z. Medarova (2016)
Superparamagnetic iron oxide nanoparticles for MR imaging and therapy: design considerations and clinical applications.
Rongrong Jin (2014)
Hybrid magnetic nanostructures (MNS) for magnetic resonance imaging applications.
M. De (2011)
Identification of gold nanoparticle in lymphocytes: a confirmation of direct intracellular penetration effect.
V. Wiwanitkit (2009)
In situ simultaneous monitoring of ATP and GTP using a graphene oxide nanosheet–based sensing platform in living cells
Y. Wang (2014)
Tatp-mediated intracellular delivery of pharmaceutical nanocarriers.
V. Torchilin (2007)
Metal‐Based Chelates and Nanosystems as MRI Contrast Agents
S. Figueiredo (2013)
Fixed bed reactor for solid-phase surface derivatization of superparamagnetic nanoparticles.
B. Steitz (2007)
Thrombospondin-1 and disease progression in dysferlinopathy
N. Urao (2017)
Binding and Internalization of Iron Oxide Nanoparticles Targeted to Nuclear Oncoprotein.
L. Knight (2010)See more