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Single-cell And Multivariate Approaches In Genetic Perturbation Screens

P. Liberali, B. Snijder, L. Pelkmans
Published 2015 · Biology, Medicine

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Large-scale genetic perturbation screens are a classical approach in biology and have been crucial for many discoveries. New technologies can now provide unbiased quantification of multiple molecular and phenotypic changes across tens of thousands of individual cells from large numbers of perturbed cell populations simultaneously. In this Review, we describe how these developments have enabled the discovery of new principles of intracellular and intercellular organization, novel interpretations of genetic perturbation effects and the inference of novel functional genetic interactions. These advances now allow more accurate and comprehensive analyses of gene function in cells using genetic perturbation screens.
This paper references
trajectory detection uncovers progression and regulatory coordination in human B cell development
G. Hinton (2014)
10.1126/SCIENCE.1065810
Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants
A. Tong (2001)
10.1038/nmeth.2069
Single cell systems biology by super-resolution imaging and combinatorial labeling
Eric Lubeck (2012)
10.1038/220266A0
Species Specificity in Growth Regulatory Effects of Cellular Interaction
H. Eagle (1968)
10.1038/nrn3025
From the stochasticity of molecular processes to the variability of synaptic transmission
Claire Ribrault (2011)
10.1038/2131102a0
Growth Regulatory Effects of Cellular Interaction
H. Eagle (1967)
10.1007/978-1-4419-1428-6_5195
Pattern Classification
P. Hart (1973)
10.1073/pnas.0808831105
Determination of cell fate selection during phage lambda infection
François St-Pierre (2008)
10.1038/msb.2010.22
Patterns of basal signaling heterogeneity can distinguish cellular populations with different drug sensitivities
D. Singh (2010)
Visualizing data using t ‐ SNE . J .
L. (2008)
reverse genetics in Drosophila melanogaster
M. D. Adams (2002)
10.1016/S0092-8674(00)00015-5
Functional Discovery via a Compendium of Expression Profiles
T. Hughes (2000)
10.1038/NCHEMBIO.2007.53
Integrating high-content screening and ligand-target prediction to identify mechanism of action.
D. W. Young (2008)
10.1038/nature08779
Systems survey of endocytosis by multiparametric image analysis
Claudio Collinet (2010)
10.1126/science.1232033
RNA-Guided Human Genome Engineering via Cas9
P. Mali (2013)
10.1016/j.cell.2005.08.031
Exploration of the Function and Organization of the Yeast Early Secretory Pathway through an Epistatic Miniarray Profile
M. Schuldiner (2005)
10.1038/nrg2918
Network medicine: a network-based approach to human disease
Albert-László Barabási (2010)
10.1016/j.cell.2014.05.010
Development and Applications of CRISPR-Cas9 for Genome Engineering
Patrick D. Hsu (2014)
10.1038/35038557
Chemical genetics: ligand-based discovery of gene function
B. Stockwell (2000)
10.1371/journal.pbio.0020379
Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets
U. Eggert (2004)
10.1126/SCIENCE.1100709
Multidimensional Drug Profiling By Automated Microscopy
Z. Perlman (2004)
10.1038/nmeth.1581
Mapping of signaling networks through synthetic genetic interaction analysis by RNAi
T. Horn (2011)
10.1007/978-1-60761-580-4_14
Dimension reduction for high-dimensional data.
L. Li (2010)
10.1126/science.1180823
The Genetic Landscape of a Cell
M. Costanzo (2010)
10.1016/j.cell.2011.03.020
Genetic Interactions in Cancer Progression and Treatment
A. Ashworth (2011)
10.1038/ncb2074
Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions
J. Connelly (2010)
10.1016/j.cell.2010.03.034
Decision Making at a Subcellular Level Determines the Outcome of Bacteriophage Infection
Lanying Zeng (2010)
10.1038/nrg886
Treasures and traps in genome-wide data sets: case examples from yeast
B. Gruenenfelder (2002)
10.1038/nrm2766
Systems biology of stem cell fate and cellular reprogramming
B. MacArthur (2009)
10.1146/annurev-biochem-060408-092949
Genomic screening with RNAi: results and challenges.
S. Mohr (2010)
10.1038/nmeth.1351
Statistical methods for analysis of high-throughput RNA interference screens
A. Birmingham (2009)
10.1038/nature09326
Functional roles for noise in genetic circuits
Avigdor Eldar (2010)
10.1038/nature08282
Population context determines cell-to-cell variability in endocytosis and virus infection
B. Snijder (2009)
10.1016/j.cell.2010.04.033
Cellular Heterogeneity: Do Differences Make a Difference?
S. Altschuler (2010)
10.1073/PNAS.95.25.14863
Cluster analysis and display of genome-wide expression patterns.
M. Eisen (1998)
10.1038/nature08781
Variability in gene expression underlies incomplete penetrance
A. Raj (2010)
10.1038/nbt.1628
Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe
D. Kim (2010)
10.1038/nmeth.2655
Predicting functional gene interactions with the hierarchical interaction score
B. Snijder (2013)
10.1038/35042517
Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
A. Fraser (2000)
10.1038/nrmicro1460
Microbial cell individuality and the underlying sources of heterogeneity
S. Avery (2006)
10.1038/nrg2085
Exploring genetic interactions and networks with yeast
C. Boone (2007)
10.1016/0092-8674(80)90128-2
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway
P. Novick (1980)
10.1126/science.1158739
Phosphorylation Networks Regulating JNK Activity in Diverse Genetic Backgrounds
C. Bakal (2008)
10.1006/EXCR.1999.4656
Isolation and characterization of a near-haploid human cell line.
M. Kotecki (1999)
10.1038/nprot.2008.211
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
D. Huang (2008)
10.1038/nrg2857
Computational solutions to large-scale data management and analysis
E. Schadt (2010)
10.1038/nmeth.2835
Multiplexed 3D Cellular Super-Resolution Imaging with DNA-PAINT and Exchange-PAINT
R. Jungmann (2014)
10.1038/nmeth.2084
Biological imaging software tools
K. Eliceiri (2012)
10.1038/nature10163
A conditional knockout resource for the genome-wide study of mouse gene function
W. Skarnes (2011)
10.1073/pnas.1018098108
A haploid genetic screen identifies the major facilitator domain containing 2A (MFSD2A) transporter as a key mediator in the response to tunicamycin
J. H. Reiling (2011)
10.1038/ncb1543
Growth factor-induced MAPK network topology shapes Erk response determining PC-12 cell fate
Silvia D. M. Santos (2007)
10.1038/nature11897
Dynamics extracted from fixed cells reveal feedback linking cell growth to cell cycle
R. Kafri (2013)
10.1126/science.1160617
Feedback Loops Shape Cellular Signals in Space and Time
Onn Brandman (2008)
10.1016/j.cell.2010.11.052
Phenotypic Landscape of a Bacterial Cell
R. J. Nichols (2011)
10.1126/science.1178955
Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens
J. Carette (2009)
10.1038/nbt.1644
A mouse knockout library for secreted and transmembrane proteins
T. Tang (2010)
10.1038/nature08460
Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1
D. Barbie (2009)
10.1073/pnas.051013498
A large-scale overexpression screen in Saccharomyces cerevisiae identifies previously uncharacterized cell cycle genes
L. Stevenson (2001)
10.1038/nature01811
Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-κB
T. Brummelkamp (2003)
Systematic mapping of genetic interaction
S. J. Dixon (2009)
10.1093/BIOINFORMATICS/BTI662
Non-transcriptional pathway features reconstructed from secondary effects of RNA interference
F. Markowetz (2005)
10.1038/ncb2764
A Screen for Morphological Complexity Identifies Regulators of Switch-like Transitions between Discrete Cell Shapes
Z. Yin (2013)
10.1101/gad.1645008
Control of cell fate by the formation of an architecturally complex bacterial community.
H. Vlamakis (2008)
10.1038/nmeth.2046
Unsupervised modeling of cell morphology dynamics for time-lapse microscopy
Q. Zhong (2012)
10.1146/annurev.genet.39.073003.114751
Systematic mapping of genetic interaction networks.
S. Dixon (2009)
10.1038/nbt.1991
Extracting a Cellular Hierarchy from High-dimensional Cytometry Data with SPADE
P. Qiu (2011)
10.1016/j.cell.2004.09.008
Robustness of Cellular Functions
J. Stelling (2004)
10.1126/science.1198704
Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum
S. Bendall (2011)
10.1162/153244303322753616
An Introduction to Variable and Feature Selection
I. Guyon (2003)
Visualizing Data using t-SNE
L. V. D. Maaten (2008)
10.1093/nar/gkq973
The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored
Damian Szklarczyk (2011)
10.1016/S0092-8674(03)00315-5
Switch-of-Function Mutants Based on Morphology Classification of Ras Superfamily Small GTPases
W. Heo (2003)
10.1038/nature08869
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
B. Neumann (2010)
10.1038/nmeth1032
Image-based multivariate profiling of drug responses from single cells
Lit-Hsin Loo (2007)
10.1038/nchembio.503
A mammalian functional-genetic approach to characterizing cancer therapeutics.
H. Jiang (2011)
How to understand the cell by breaking it – methods and software for network analysis of gene perturbation screens
F. Markowetz (2010)
10.1186/gb-2006-7-10-r100
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
A. Carpenter (2006)
10.1016/j.cell.2013.02.022
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Lei S. Qi (2013)
10.1038/nbt.2317
Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators
B. Bodenmiller (2012)
10.1016/j.cell.2006.06.044
Matrix Elasticity Directs Stem Cell Lineage Specification
A. Engler (2006)
10.1016/j.cell.2014.04.005
Single-Cell Trajectory Detection Uncovers Progression and Regulatory Coordination in Human B Cell Development
S. Bendall (2014)
10.1126/SCIENCE.1091266
Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells
M. Boutros (2004)
10.1126/science.1246981
Genetic Screens in Human Cells Using the CRISPR-Cas9 System
T. Wang (2014)
10.1038/nrg2364
The art and design of genetic screens: RNA interference
M. Boutros (2008)
10.1016/j.molcel.2012.11.023
Dissection of DNA damage responses using multiconditional genetic interaction maps.
Aude Guénolé (2013)
10.1073/pnas.0807038105
Characterizing heterogeneous cellular responses to perturbations
M. Slack (2008)
10.1039/B515335B
Small molecules in an RNAi world.
U. Eggert (2006)
10.1038/474262a
Mouse library set to be knockout
Elie Dolgin (2011)
10.1016/J.GDE.2004.07.010
Genome-wide high-throughput screens in functional genomics.
Adam A. Friedman (2004)
10.1038/nature00935
Functional profiling of the Saccharomyces cerevisiae genome
G. Giaever (2002)
10.1038/nbt.1755
A TALE nuclease architecture for efficient genome editing
J. C. Miller (2011)
10.1038/nrm3212
Imaging the coordination of multiple signalling activities in living cells
C. M. Welch (2011)
10.1016/j.cell.2014.09.029
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
L. Gilbert (2014)
10.1073/pnas.1101134108
Systems-level analysis of microbial community organization through combinatorial labeling and spectral imaging
A. Valm (2011)
10.1016/S1534-5807(02)00325-8
Sequential activation of signaling pathways during innate immune responses in Drosophila.
M. Boutros (2002)
10.1093/bioinformatics/btp524
CellClassifier: supervised learning of cellular phenotypes
P. Rämö (2009)
10.1038/nature03876
Predictive models of molecular machines involved in Caenorhabditis elegans early embryogenesis
Kristin C. Gunsalus (2005)
10.1093/bioinformatics/bts634
Inference of gene regulatory networks from genome-wide knockout fitness data
L. Wang (2013)
10.1126/science.1222161
Using Cell-to-Cell Variability—A New Era in Molecular Biology
L. Pelkmans (2012)
10.1038/ncb2510
Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway
J. C. Simpson (2012)
10.1093/BIOINFORMATICS/BTM178
Nested effects models for high-dimensional phenotyping screens
F. Markowetz (2007)
10.1371/journal.pone.0008471
A Direct Phenotypic Comparison of siRNA Pools and Multiple Individual Duplexes in a Functional Assay
B. Parsons (2009)
10.1038/nrg2842
Genome editing with engineered zinc finger nucleases
F. Urnov (2010)
results and challenges
S. Mohr (2010)
10.1126/scitranslmed.3007361
Cancer Cell Profiling by Barcoding Allows Multiplexed Protein Analysis in Fine-Needle Aspirates
Adeeti V. Ullal (2014)
10.1038/nrg752
From sequence to phenotype: reverse genetics in drosophila melanogaster
M. Adams (2002)
10.1126/science.1140324
Quantitative Morphological Signatures Define Local Signaling Networks Regulating Cell Morphology
C. Bakal (2007)
10.1038/msb.2010.27
Automated identification of pathways from quantitative genetic interaction data
A. Battle (2010)
10.1186/gb-2006-7-7-r66
Analysis of cell-based RNAi screens
M. Boutros (2006)
10.1038/nature03998
Regulated cell-to-cell variation in a cell-fate decision system
A. Colman-Lerner (2005)
10.1073/pnas.1300136110
Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue
M. Gerdes (2013)
10.1038/ncomms2635
Quantum dot imaging platform for single-cell molecular profiling
P. Zrazhevskiy (2013)
10.1073/pnas.0808843106
Scoring diverse cellular morphologies in image-based screens with iterative feedback and machine learning
T. Jones (2009)
10.1126/science.1195618
Rewiring of Genetic Networks in Response to DNA Damage
S. Bandyopadhyay (2010)
10.1126/science.1247005
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
O. Shalem (2014)
10.1038/nature06952
Mechanism of shape determination in motile cells
Kinneret Keren (2008)
10.1038/msb.2011.99
Differential network biology
T. Ideker (2012)
insights from imaging
L. Dehmelt (2010)
custom‐designed molecular scissors for genome engineering of plant and mammalian cells
Durai (2005)
10.1093/nar/gkq1039
Pathway Commons, a web resource for biological pathway data
Ethan G. Cerami (2011)
10.1038/nature10448
Derivation of haploid embryonic stem cells from mouse embryos
M. Leeb (2011)
10.1038/nrm3044
Origins of regulated cell-to-cell variability
B. Snijder (2011)
‐ surface variation in yeast
S. V. Avery (2004)
10.1038/nmeth.1375
An approach for extensibly profiling the molecular states of cellular subpopulations
Lit-Hsin Loo (2009)
10.1038/msb.2012.59
RNAi screening reveals a large signaling network controlling the Golgi apparatus in human cells
Joanne Chia (2012)
case examples from yeast
B. Grunenfelder (2002)
technique for real time single cell multitarget immunoassay based on inductively coupled plasma time‐of‐flight mass spectrometry
Bandura (2009)
10.1038/nrm2903
Spatial organization of intracellular communication: insights from imaging
L. Dehmelt (2010)
10.1038/nmeth.2657
Image-based transcriptomics in thousands of single human cells at single-molecule resolution
Nico Battich (2013)
10.1038/35042526
Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
P. Gönczy (2000)
10.1126/science.1149185
Profiling Essential Genes in Human Mammary Cells by Multiplex RNAi Screening
J. M. Silva (2008)
10.1021/ac901049w
Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry.
D. Bandura (2009)
10.1371/journal.pcbi.1000655
How to Understand the Cell by Breaking It: Network Analysis of Gene Perturbation Screens
F. Markowetz (2010)
10.1038/msb.2012.9
Single-cell analysis of population context advances RNAi screening at multiple levels
Berend Snijder (2012)
10.1126/science.1231143
Multiplex Genome Engineering Using CRISPR/Cas Systems
L. Công (2013)
10.1038/nmeth.2436
Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping
C. Laufer (2013)
10.1016/j.molcel.2012.04.001
Cell differentiation within a yeast colony: metabolic and regulatory parallels with a tumor-affected organism.
M. Čáp (2012)
10.1038/nature02370
A resource for large-scale RNA-interference-based screens in mammals
P. Paddison (2004)
10.1126/SCIENCE.1081403
Identification of Hedgehog Pathway Components by RNAi in Drosophila Cultured Cells
L. Lum (2003)
10.1038/150563A0
Canalization of Development and the Inheritance of Acquired Characters
C. Waddington (1942)
10.1038/nrg1248
Systematic genome-wide screens of gene function
A. Carpenter (2004)
10.1016/j.cell.2013.01.030
A Systematic Mammalian Genetic Interaction Map Reveals Pathways Underlying Ricin Susceptibility
M. Bassik (2013)
10.1038/nature03571
Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis
L. Pelkmans (2005)
10.1126/SCIENCE.1070919
Stochastic Gene Expression in a Single Cell
M. Elowitz (2002)
10.1073/PNAS.0509861103
Genomewide gain-of-function genetic screen identifies functionally active genes in mouse embryonic stem cells.
Moshe Pritsker (2006)
10.1038/NCHEMBIO1207-739
Recognizing and exploiting differences between RNAi and small-molecule inhibitors.
W. Weiss (2007)
10.1126/SCIENCE.1091317
Global Mapping of the Yeast Genetic Interaction Network
A. Tong (2004)
10.1093/nar/gki912
Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells
S. Durai (2005)
10.3410/f.1020412.232570
Faculty Opinions recommendation of Single cell profiling of potentiated phospho-protein networks in cancer cells.
J. Woodgett (2004)
10.1016/j.cell.2004.06.028
Single Cell Profiling of Potentiated Phospho-Protein Networks in Cancer Cells
J. Irish (2004)
10.1126/science.1216379
Using Gene Expression Noise to Understand Gene Regulation
B. Munsky (2012)
10.1016/S1097-2765(03)00348-4
Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening.
P. Aza-Blanc (2003)
10.1016/S0076-6879(10)70009-4
Quantitative genetic interaction mapping using the E-MAP approach.
S. Collins (2010)
10.1038/nature08012
Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis
S. Spencer (2009)
10.1016/j.ceb.2011.09.005
Robust selection of sensory organ precursors by the Notch-Delta pathway.
Omer Barad (2011)
10.1038/msb.2010.12
Pre-dispositions and epigenetic inheritance in the Escherichia coli lactose operon bistable switch
L. Robert (2010)
10.1038/nbt1250
Analyzing proteome topology and function by automated multidimensional fluorescence microscopy
W. Schubert (2006)
10.1016/j.cub.2010.12.047
Cell-to-Cell Variability in PI3K Protein Level Regulates PI3K-AKT Pathway Activity in Cell Populations
T. Yuan (2011)
10.1016/j.cell.2014.04.029
A Hierarchical Map of Regulatory Genetic Interactions in Membrane Trafficking
P. Liberali (2014)
10.1002/JCP.1040750107
Flattening, movement and control of division of epithelial‐like cells
L. Castor (1970)
10.1038/nmeth.2398
Quantitative genetic-interaction mapping in mammalian cells
Assen Roguev (2013)
10.1016/S0092-8674(04)00118-7
Genetic and Epigenetic Regulation of the FLO Gene Family Generates Cell-Surface Variation in Yeast
Adrian Halme (2004)
10.1007/978-1-60761-588-0
RNA Interference
Wei-Ping Min (2010)
10.1186/1471-2105-9-482
CellProfiler Analyst: data exploration and analysis software for complex image-based screens
T. Jones (2008)
10.1038/nmeth.2609
A reversible gene trap collection empowers haploid genetics in human cells
T. Bürckstümmer (2013)
10.1126/SCIENCE.285.5429.901
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.
E. Winzeler (1999)
10.1016/j.cell.2008.12.039
Functional Organization of the S. cerevisiae Phosphorylation Network
D. Fiedler (2009)



This paper is referenced by
10.1101/761106
KDML: a machine-learning framework for inference of multi-scale gene functions from genetic perturbation screens
H. Sailem (2019)
10.1007/978-1-4939-6993-7_5
Cell Cycle Resolved Measurements of Poly(ADP-Ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry.
J. Michelena (2017)
Probing insecticide biology using Drosophila melanogaster
S. Denecke (2017)
METHOD Open Access
L. Maretty (2014)
10.1038/s41467-018-05031-9
Analysis of PARP inhibitor toxicity by multidimensional fluorescence microscopy reveals mechanisms of sensitivity and resistance
J. Michelena (2018)
10.1091/mbc.E15-07-0466
Single-cell phenomics in budding yeast
Y. Ohya (2015)
10.1101/139048
Identifying parameters of host cell vulnerability during Salmonella infection by quantitative image analysis and modeling
J. Voznica (2017)
10.1101/361410
Unsupervised phenotypic analysis of cellular images with multi-scale convolutional neural networks
William J. Godinez (2018)
10.1101/545335
Morphological deconvolution of beta-lactam polyspecificity in E. coli
William J. Godinez (2019)
10.1016/j.cell.2015.11.007
Microscopy-Based High-Content Screening
M. Boutros (2015)
10.14288/1.0348261
A systems biology study of alternative splicing regulations and functions
Seyed Alborz Mazloomian (2017)
10.1111/febs.13251
High‐throughput screens in mammalian cells using the CRISPR‐Cas9 system
Jingyu Peng (2015)
10.15252/msb.20199083
KCML: a machine‐learning framework for inference of multi‐scale gene functions from genetic perturbation screens
H. Sailem (2020)
10.1016/j.tibs.2018.10.010
A Primer on Data Analytics in Functional Genomics: How to Move from Data to Insight?
P. Grabowski (2019)
10.21769/BioProtoc.3017
High-throughput Microscopic Analysis of Salmonella Invasion of Host Cells.
J. Voznica (2018)
10.1016/B978-0-12-800781-5.00019-0
Synopsis and Epilogue: Neural Surface Antigen Studies in Biology and Biomedicine—What We Have Learned and What the Future May Hold
J. Pruszak (2015)
10.15252/msb.20177573
Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events in Escherichia coli
M. Campos (2018)
Dosages utilisés pour le profilage de perturbation massivement combinatoire et la reconstruction de circuit cellulaire
A. Regev (2016)
10.1016/J.CHEMPR.2018.06.011
High-Speed Imaging Meets Single-Cell Analysis
H. Mikami (2018)
10.1093/bioinformatics/btw252
Classifying and segmenting microscopy images with deep multiple instance learning
Oren Z. Kraus (2016)
Robotic Manipulation and Characterization of Cells for Drug Screen and Clinical IVF Applications
Jun Liu (2016)
10.1002/cphc.201500987
Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging.
Jérôme Querard (2016)
10.1038/s41467-019-09234-6
Metascape provides a biologist-oriented resource for the analysis of systems-level datasets
Yingyao Zhou (2019)
10.1016/j.ymeth.2015.10.007
A metric and workflow for quality control in the analysis of heterogeneity in phenotypic profiles and screens.
A. Gough (2016)
10.1038/ncomms9390
Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method
Jia-Ren Lin (2015)
10.1177/1087057115583037
A Multivariate Computational Method to Analyze High-Content RNAi Screening Data
Jonathan Rameseder (2015)
10.1039/c6mb00701e
Proteomics profiling of interactome dynamics by colocalisation analysis (COLA)† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6mb00701e Click here for additional data file. Click here for additional data file.
Faraz K Mardakheh (2016)
10.1101/155036
Proposal for minimum information guidelines to report and reproduce results of particle tracking and motion analysis
A. Rigano (2017)
10.1038/s41571-019-0273-6
A systems approach to clinical oncology uses deep phenotyping to deliver personalized care
James T. Yurkovich (2019)
10.1007/978-3-030-41862-5_86
A Deep Learning Approach for Segmenting Time-Lapse Phase Contrast Images of NIH 3T3 Fibroblast Cells
Aruna Kumari Kakumani (2020)
10.3109/10409238.2015.1135868
Computer vision for high content screening
Oren Z. Kraus (2016)
10.1039/c8cp03953d
Light-assisted dynamic titration: from theory to an experimental protocol.
A. Pellissier-Tanon (2018)
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