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Dipropylcyclopentylxanthine Triggers Apoptosis In Jurkat T Cells By A Receptor-independent Mechanism

M. Mirabet, J. Mallol, C. Lluis, R. Franco
Published 1997 · Biology, Medicine

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1,3-Dipropyl-8-cyclopentylxanthine (DPCPX), a xanthine analog used as selective antagonist of adenosine receptors, caused apoptosis in a human leukemia T cell line. Jurkat cells treated with DPCPX underwent apoptosis as demonstrated by flow cytometry, by DNA fragmentation and by accumulation of histones, H2A, H2B, H3 and H4, in the nucleoplasm of cells. Cell cycle and cell sorting analyses indicated an arrest of cells in G2/M followed by the appearance of apoptotic cells in G1 and G2/M phases. The mechanism of programmed cell death does not seem to be mediated by signal transduction events at the plasma membrane since it did not involve activation of cell membrane receptors and modification of the intracellular levels of Ca2+ or cAMP. Apoptosis by incorporation into DNA of a derivative of DPCPX is suggested in basis of the presence of radioactivity label in the DNA obtained from cells preincubated with [3H]DPCPX.
This paper references
Cell cycle control of apoptosis in human leukemic T cells.
L. Zhu (1995)
10.1016/0167-5699(90)90048-E
Cellular signalling in programmed cell death (apoptosis).
D. McConkey (1990)
10.1016/0042-6822(91)90554-O
The cytopathic effect of HIV is associated with apoptosis.
A. Laurent-Crawford (1991)
10.1002/CYTO.990170108
A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells.
F. Belloc (1994)
10.1002/CYTO.990140603
Increased membrane permeability of apoptotic thymocytes: a flow cytometric study.
M. Ormerod (1993)
10.1146/ANNUREV.CB.02.110186.001001
Core particle, fiber, and transcriptionally active chromatin structure.
D. S. Pederson (1986)
10.1042/BJ3040877
Adenosine stimulates DNA fragmentation in human thymocytes by Ca(2+)-mediated mechanisms.
Z. Szondy (1994)
10.1016/0167-5699(90)90047-D
Death and transfiguration of cortical thymocytes: a reconsideration.
E. Rothenberg (1990)
10.1016/0167-4889(91)90120-M
CD3-dependent increase in cyclic AMP in human T-cells following stimulation of the CD2 receptor.
A. Kvanta (1991)
10.1182/BLOOD.V81.1.143.BLOODJOURNAL811143
Induction of apoptotic cell death in chronic lymphocytic leukemia by 2-chloro-2′-deoxyadenosine and 9-β-D-arabinosyl-2-fluoroadenine
L. Robertson (1993)
10.1007/978-1-4899-2638-8_1
Biochemical approach to new medications.
G. Hitchings (1992)
10.1007/978-1-4615-2421-2_43
The Involvement of the Cell Cycle in Apoptosis
A. Eastman (1994)
Apoptosis . Immunol
JJ Cohen (1993)
10.1006/EXCR.1994.1196
Apoptosis induced by adenosine in human leukemia HL-60 cells.
Y. Tanaka (1994)
10.1007/978-1-4899-2638-8_3
2-Chlorodeoxyadenosine chemotherapy triggers programmed cell death in normal and malignant lymphocytes.
C. Carrera (1991)
Calcium-dependent killing of immature thymocytes by stimulation via the CD3/T cell receptor complex.
D. McConkey (1989)
10.1111/j.1476-5381.1983.tb10053.x
Inhibition by xanthine derivatives of adenosine receptor‐stimulated cyclic adenosine 3′, 5′‐monophosphate accumulation in rat and guinea‐pig thymocytes
B. Fredholm (1983)
The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents.
W. Gorczyca (1993)
Viability measurement using spectrum shift in Hoechst stained cells
JW Ellwart (1990)
10.1016/0167-5699(86)90152-0
Death and the cell.
E. Duvall (1986)
Template 2-chloro-2'-deoxyadenosine monophosphate inhibits in vitro DNA synthesis.
P. Hentosh (1994)
10.1002/CYTO.990130802
Features of apoptotic cells measured by flow cytometry.
Z. Darzynkiewicz (1992)
10.1016/0167-4889(92)90048-G
Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry.
C. Dive (1992)
10.1016/s0021-9258(19)34118-3
Adenosine receptor-mediated accumulation of cyclic AMP-induced T-lymphocyte death through internucleosomal DNA cleavage.
H. Kizaki (1990)



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