Online citations, reference lists, and bibliographies.
← Back to Search

MicroRNA Genes Are Transcribed By RNA Polymerase II

Yoontae Lee, Minju Kim, Jinju Han, Kyu‐Hyun Yeom, S. Lee, S. H. Baek, V. Kim
Published 2004 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
MicroRNAs (miRNAs) constitute a large family of noncoding RNAs that function as guide molecules in diverse gene silencing pathways. Current efforts are focused on the regulatory function of miRNAs, while little is known about how these unusual genes themselves are regulated. Here we present the first direct evidence that miRNA genes are transcribed by RNA polymerase II (pol II). The primary miRNA transcripts (pri‐miRNAs) contain cap structures as well as poly(A) tails, which are the unique properties of class II gene transcripts. The treatment of human cells with α‐amanitin decreased the level of pri‐miRNAs at a concentration that selectively inhibits pol II activity. Furthermore, chromatin immunoprecipitation analyses show that pol II is physically associated with a miRNA promoter. We also describe, for the first time, the detailed structure of a miRNA gene by determining the promoter and the terminator of mir‐23a∼27a∼24‐2. These data indicate that pol II is the main, if not the only, RNA polymerase for miRNA gene transcription. Our study offers a basis for understanding the structure and regulation of miRNA genes.
This paper references
10.1002/j.1460-2075.1994.tb06369.x
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high‐affinity binding sites in pre‐mRNA splicing.
C. Burd (1994)
10.1101/GAD.862301
RNA interference is mediated by 21- and 22-nucleotide RNAs.
S. Elbashir (2001)
10.1126/SCIENCE.1065329
An Extensive Class of Small RNAs in Caenorhabditis elegans
R. Lee (2001)
10.1126/SCIENCE.1062961
A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA
G. Hutvágner (2001)
10.1126/SCIENCE.1062039
A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegans
S. W. Knight (2001)
10.1101/GAD.927801
Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans.
R. Ketting (2001)
10.1016/S0378-1119(01)00612-6
Identification and characterization of human BIC, a gene on chromosome 21 that encodes a noncoding RNA.
W. Tam (2001)
10.1126/SCIENCE.1065062
An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans
N. Lau (2001)
10.1016/S0092-8674(01)00431-7
Genes and Mechanisms Related to RNA Interference Regulate Expression of the Small Temporal RNAs that Control C. elegans Developmental Timing
A. Grishok (2001)
10.1038/35053110
Role for a bidentate ribonuclease in the initiation step of RNA interference
E. Bernstein (2001)
10.1126/SCIENCE.1064921
Identification of Novel Genes Coding for Small Expressed RNAs
M. Lagos-Quintana (2001)
Identification and characterization of human BIC, a gene on chromosome 21 that encodes a noncoding RNA. Gene
W Tam (2001)
10.1016/S1097-2765(02)00541-5
Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells.
Y. Zeng (2002)
10.1101/GAD.974702
miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.
Z. Mourelatos (2002)
10.1093/emboj/cdf476
MicroRNA maturation: stepwise processing and subcellular localization
Yoontae Lee (2002)
Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappaB and beta-amyloid precursor protein.
S. Baek (2002)
10.1016/S0092-8674(02)00809-7
Exchange of N-CoR Corepressor and Tip60 Coactivator Complexes Links Gene Expression by NF-κB and β-Amyloid Precursor Protein
S. H. Baek (2002)
10.1016/S0092-8674(03)00801-8
Functional siRNAs and miRNAs Exhibit Strand Bias
A. Khvorova (2003)
10.1105/tpc.016238
Regulation of Flowering Time and Floral Organ Identity by a MicroRNA and Its APETALA2-Like Target Genes Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.016238.
M. Aukerman (2003)
10.1146/ANNUREV.BIOCHEM.72.121801.161520
The RNA polymerase II core promoter.
S. Smale (2003)
10.1016/S0012-1606(03)00202-1
The time of appearance of the C. elegans let-7 microRNA is transcriptionally controlled utilizing a temporal regulatory element in its promoter.
Steven M. Johnson (2003)
10.1016/S0092-8674(03)00759-1
Asymmetry in the Assembly of the RNAi Enzyme Complex
D. Schwarz (2003)
10.1016/S0092-8674(03)00231-9
bantam Encodes a Developmentally Regulated microRNA that Controls Cell Proliferation and Regulates the Proapoptotic Gene hid in Drosophila
J. Brennecke (2003)
10.1186/gb-2003-4-7-403
EST analyses predict the existence of a population of chimeric microRNA precursor-mRNA transcripts expressed in normal human and mouse tissues
N. Smalheiser (2003)
10.1016/S0092-8674(03)01018-3
Prediction of Mammalian MicroRNA Targets
B. P. Lewis (2003)
10.1261/RNA.2183803
A uniform system for microRNA annotation.
V. Ambros (2003)
10.1101/GAD.1158803
Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs.
R. Yi (2003)
10.1038/nature01957
The nuclear RNase III Drosha initiates microRNA processing
Yoontae Lee (2003)
10.1016/S0092-8674(04)00045-5
MicroRNAs Genomics, Biogenesis, Mechanism, and Function
D. Bartel (2004)
10.1016/J.TCB.2004.02.006
MicroRNA precursors in motion: exportin-5 mediates their nuclear export.
V. Kim (2004)
10.1261/RNA.5167604
Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs.
M. T. Bohnsack (2004)
10.1126/SCIENCE.1090599
Nuclear Export of MicroRNA Precursors
E. Lund (2004)
10.1126/SCIENCE.1091903
MicroRNAs Modulate Hematopoietic Lineage Differentiation
Chang-zheng Chen (2004)
10.1126/SCIENCE.1096781
Identification of Virus-Encoded MicroRNAs
S. Pfeffer (2004)
10.1016/J.CEB.2004.04.003
miRNAs on the move: miRNA biogenesis and the RNAi machinery.
E. Murchison (2004)



This paper is referenced by
10.1186/s12935-021-02144-y
Noncoding RNAs involved in the STAT3 pathway in glioma
Zheng Bian (2021)
10.1002/bit.27821
One vector‐based method to verify predicted plant miRNAs, target sequences, and function modes
Hui Zhou (2021)
10.1111/aos.14845
MicroRNAs and the HIF/VEGF axis in ocular neovascular diseases.
F. Plastino (2021)
10.1016/j.mce.2021.111175
MicroRNAs and thyroid hormone action
A. Aranda (2021)
10.1007/s11033-021-06152-z
A review on EBV encoded and EBV-induced host microRNAs expression profile in different lymphoma types.
S. Soltani (2021)
10.3390/cancers13071742
Roles of microRNAs in Regulating Cancer Stemness in Head and Neck Cancers
Melysa Fitriana (2021)
10.1080/14397595.2020.1868674
miRNAs as attractive diagnostic and therapeutic targets for Familial Mediterranean Fever
H. M. Okuyan (2021)
10.1002/cac2.12164
YB1 regulates miR‐205/200b‐ZEB1 axis by inhibiting microRNA maturation in hepatocellular carcinoma
Xiumei Liu (2021)
10.1016/J.PLGENE.2021.100277
Role of microRNAs in mediating biotic and abiotic stress in plants
Madhabendra Mohon Kar (2021)
10.3389/fgene.2021.739849
Profiling of MicroRNAs in Midguts of Plutella xylostella Provides Novel Insights Into the Bacillus thuringiensis Resistance
Jie Yang (2021)
10.1007/S11831-021-09556-Z
A Systematic Review of Applications of Machine Learning in Cancer Prediction and Diagnosis
Aman Sharma (2021)
10.1016/j.biopha.2021.111528
MicroRNA: A signature for cancer progression.
B. M. Hussen (2021)
10.1016/j.biopha.2021.111753
New epigenetic players in stroke pathogenesis: From non-coding RNAs to exosomal non-coding RNAs
Maryam Mahjoubin-Tehran (2021)
10.3390/cancers13071555
Noncoding RNAs in Glioblastoma: Emerging Biological Concepts and Potential Therapeutic Implications
Uswa Shahzad (2021)
10.3390/ijms22062910
The Roles of MicroRNAs in Male Infertility
M. Barbu (2021)
10.1007/s00299-020-02660-7
Regulation of pri-MIRNA processing: mechanistic insights into the miRNA homeostasis in plant.
Jayanti Jodder (2021)
10.1159/000515750
Adverse Maternal Environment Alters MicroRNA-10b-5p Expression and Its Epigenetic Profile Concurrently with Impaired Hippocampal Neurogenesis in Male Mouse Hippocampus
X. Ke (2021)
10.3390/ijms22147716
Non-Coding RNAs: Novel Players in Insulin Resistance and Related Diseases
C. Formichi (2021)
10.3390/cancers13051085
Small Non-Coding-RNA in Gynecological Malignancies
S. Dwivedi (2021)
10.3389/fmicb.2021.714409
microRNAs, the Link Between Dengue Virus and the Host Genome
Yinghua Su (2021)
10.3389/fimmu.2021.623973
Mesenchymal Stromal Cell-Derived Extracellular Vesicles Regulate the Mitochondrial Metabolism via Transfer of miRNAs
C. Loussouarn (2021)
10.1038/s41598-021-89363-5
Application of the targeted sequencing approach reveals the single nucleotide polymorphism (SNP) repertoire in microRNA genes in the pig genome
K. Pawlina-Tyszko (2021)
10.1080/1040841X.2021.1907739
Modulating host gene expression via gut microbiome–microRNA interplay to treat human diseases
D. Mody (2021)
10.1016/j.jbc.2021.100707
CtIP suppresses primary microRNA maturation and promotes metastasis of colon cancer cells in a xenograft mouse model
Jianping Ren (2021)
10.22074/cellj.2021.7109
MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis
Halimeh Rezaei (2021)
10.3390/ijms22137153
Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis
S. M. M. Mehdi (2021)
10.3390/ijms22147526
MicroRNAs in Epithelial–Mesenchymal Transition Process of Cancer: Potential Targets for Chemotherapy
F. Peng (2021)
10.1134/S0006297921070014
MicroRNAs in Cancer: From Gene Expression Regulation to the Metastatic Niche Reprogramming.
E. Semina (2021)
10.3390/ijms22062935
Cyclin-Dependent Kinases (CDK) and Their Role in Diseases Development–Review
Paweł Łukasik (2021)
10.1007/s11033-021-06290-4
The role of miRNA in plant-virus interaction: a review.
Anteneh Ademe Mengistu (2021)
10.1093/bib/bbab075
MicroRNA annotation in plants: current status and challenges.
Yongxin Zhao (2021)
10.1055/a-1478-2105
Do miRNAs Have a Role in Platelet Function Regulation?
A. Garcia (2021)
See more
Semantic Scholar Logo Some data provided by SemanticScholar