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Induction Of Caspase-3-like Activity In Rice Following Release Of Cytochrome-f From The Chloroplast And Subsequent Interaction With The Ubiquitin-Proteasome System

H. Wang, X. Zhu, Huan Li, J. Cui, C. Liu, X. Chen, Wei Zhang
Published 2014 · Biology, Medicine

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It has been known that the process of leaf senescence is accompanied by programmed cell death (PCD), and the previous study indicated that dark-induced senescence in detached leaves from rice led to the release of cytochrome f (Cyt f) from chloroplast into the cytoplasm. In this study, the effects of Cyt f on PCD were studied both in vitro and in vivo. In a cell-free system, purified Cyt f activated caspase-3-like protease and endonuclease OsNuc37, and induced DNA fragmentation. Furthermore, Cyt f-induced caspase-3-like activity could be inhibited by MG132, which suggests that the activity was attributed to the 26S proteasome. Conditional expression of Cyt f in the cytoplasm could also activate caspase-3-like activity and DNA fragmentation. Fluorescein diacetate staining and annexin V-FITC/PI double staining demonstrated that Cyt f expression in cytoplasm significantly increased the percentage of PCD protoplasts. Yeast two-hybrid screening showed that Cyt f might interact with E3-ubiquitin ligase and RPN9b, the subunits of the ubiquitin proteasome system (UPS), and other PCD-related proteins. Taken together, these results suggest that the released Cyt f from the chloroplast into the cytoplasm might activate or rescue caspase-3-like activity by interacting with the UPS, ultimately leading to the induction of PCD.
This paper references
A novel membrane fusion-mediated plant immunity against bacterial pathogens.
N. Hatsugai (2009)
Signal transduction in response to excess light: getting out of the chloroplast.
P. Mullineaux (2002)
Studies on differential nuclear translocation mechanism and assembly of the three subunits of the Arabidopsis thaliana transcription factor NF-Y.
Dieter Hackenberg (2012)
DEVDase activity is induced in potato leaves during Phytophthora infestans infection.
M. B. Fernández (2012)
A Bacterial Inhibitor of Host Programmed Cell Death Defenses Is an E3 Ubiquitin Ligase
R. Janjusevic (2006)
Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity.
R. B. Abramovitch (2006)
A zinc-dependent nuclear endonuclease is responsible for DNA laddering during salt-induced programmed cell death in root tip cells of rice.
Ai-Liang Jiang (2008)
Smac/Diablo Antagonizes Ubiquitin Ligase Activity of Inhibitor of Apoptosis Proteins*
E. Creagh (2004)
Classes of programmed cell death in plants, compared to those in plants
W. G. van Doorn (2011)
Rice NON-YELLOW COLORING1 Is Involved in Light-Harvesting Complex II and Grana Degradation during Leaf Senescence[W]
M. Kusaba (2007)
STAND, a class of P-loop NTPases including animal and plant regulators of programmed cell death: multiple, complex domain architectures, unusual phyletic patterns, and evolution by horizontal gene transfer.
D. Leipe (2004)
Intracellular Nucleotides Act as Critical Prosurvival Factors by Binding to Cytochrome C and Inhibiting Apoptosome
D. Chandra (2006)
Vacuolar processing enzyme: an executor of plant cell death.
I. Hara-Nishimura (2005)
Ultraviolet-C Overexposure Induces Programmed Cell Death in Arabidopsis, Which Is Mediated by Caspase-like Activities and Which Can Be Suppressed by Caspase Inhibitors, p35 and Defender against Apoptotic Death*
A. Danon (2004)
Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in Nicotiana tabacum.
S. S. Lee (2003)
Subcellular distribution of enzymes of the oxidative pentose phosphate pathway in root and leaf tissues
P. M. Debnam (1999)
A balance between NF-Y and p53 governs the pro- and anti-apoptotic transcriptional response
P. Benatti (2008)
A Nucleotide Phosphatase Activity in the Nucleotide Binding Domain of an Orphan Resistance Protein from Rice*
Stepan Fenyk (2011)
Arabidopsis Type I Metacaspases Control Cell Death
N. S. Coll (2010)
Activation of caspase-like proteases and induction of apoptosis by isopentenyladenosine in tobacco BY-2 cells
P. Mlejnek (2002)
multiple, complex domain architectures, unusual phyletic patterns, and evolution by horizontal gene transfer
D. D. Leipe (2004)
The Authors Declare No Competing Financial Interests. 2 Significance Statement
Pietro Baldelli
Self-incompatibility triggers programmed cell death in Papaver pollen
S. Thomas (2004)
Proteolytic removal of the C-terminal transmembrane region of cytochrome f during extraction from turnip and charlock leaves generates a water-soluble monomeric form of the protein.
J. Gray (1994)
Staphylococcus aureus CidA and LrgA proteins exhibit holin-like properties.
Dev K Ranjit (2011)
getting out of the chloroplast
P. Mullineaux (2002)
Preparation and transformation of monocot protoplasts.
C. Maas (1995)
Cytochrome f: Structure, function and biosynthesis
J. Gray (2004)
structure, function and biosynthesis
Gray (1992)
Phytaspase, a relocalisable cell death promoting plant protease with caspase specificity
N. Chichkova (2010)
Programmed cell death in plants: lessons from bacteria?
J. Wang (2013)
The proteasome is responsible for caspase-3-like activity during xylem development.
Jiajia Han (2012)
Purification and Characterization of Serine Proteases That Exhibit Caspase-Like Activity and Are Associated with Programmed Cell Death in Avena sativa
Warren C. Coffeen (2004)
lessons from bacteria? Trends Plant Sci
J. Wang (2013)
Classes of programmed cell death in plants, compared to those in animals
W. Doorn (2011)
Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures
S. M. Doyle (2010)
High Nuclear Yields from Protoplasts of Several Plants
P. Saxena (1985)
Smac, a Mitochondrial Protein that Promotes Cytochrome c–Dependent Caspase Activation by Eliminating IAP Inhibition
C. Du (2000)
Evidence for cytochrome f involvement in eggplant cell death induced by palmitoleic acid
J. Peters (2005)
IAPs block apoptotic events induced by caspase‐8 and cytochrome c by direct inhibition of distinct caspases
Q. Deveraux (1998)
Ubiquitin-protein ligase activity of X-linked inhibitor of apoptosis protein promotes proteasomal degradation of caspase-3 and enhances its anti-apoptotic effect in Fas-induced cell death
Y. Suzuki (2001)
The presence and subcellular localization of caspase 3‐like proteinases in plant cells
H. Korthout (2000)
Chlorella saccharophila cytochrome f and its involvement in the heat shock response
Anna Zuppini (2009)
nucleotides act as critical prosurvival factors by binding to cytochromeC and inhibiting apoptosome
D. Chandra (2006)
Chloroplasts of Arabidopsis Are the Source and a Primary Target of a Plant-Specific Programmed Cell Death Signaling Pathway[W]
C. Kim (2012)
Activation of the Programmed Cell Death Pathway by Inhibition of Proteasome Function in Plants*
M. Kim (2003)
Acknowledgments This work was support by the National Natural Science Foundation of China 31171326
Classes of programmed cell death in plants, compared to those in plants
W G Van Doorn (2011)

This paper is referenced by
Pulsed electric field (PEF)-assisted protein recovery from microalgae biomass for food and feed applications
D. Scherer (2018)
Licensed to Kill: Mitochondria, Chloroplasts, and Cell Death
O. V. Aken (2015)
Spermidine sprays alleviate the water deficit-induced oxidative stress in finger millet (Eleusine coracana L. Gaertn.) plants
L. Satish (2018)
Chapter 2 Mitochondrion and Chloroplast Regulation of Plant Programmed Cell Death
Theresa J. Reape (2018)
Glucosidase II β-subunit, a novel substrate for caspase-3-like activity in rice, plays as a molecular switch between autophagy and programmed cell death
J. Cui (2016)
Flavonoids and darkness lower PCD in senescing Vitis vinifera suspension cell cultures
A. Bertolini (2016)
Programmed cell death in plants: A chloroplastic connection
Vivek Ambastha (2015)
Caspase-like proteases regulate aluminum-induced programmed cell death in peanut
Shaochang Yao (2016)
Effect of an MG132-Sustained Drug Delivery Capsular Ring on the Inhibition of Posterior Capsule Opacification in a Rabbit Model
BaoXuan (2017)
Redox State as a Central Regulator of Plant-Cell Stress Responses
D. Gupta (2016)
Plant Death: Short and Long Life Span to Immortality
S. S. Pandey (2019)
Cytochrome c, a hub linking energy, redox, stress and signaling pathways in mitochondria and other cell compartments.
Elina Welchen (2016)
Identification of Differentially Expressed Genes during Lace Plant Leaf Development
Gaolathe Rantong (2016)
Abiotic Stress-Induced Redox Changes and Programmed Cell Death in Plants—A Path to Survival or Death?
S. R. Kumar (2016)
Mitochondrion and Chloroplast Regulation of Plant Programmed Cell Death
Theresa J. Reape (2015)
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