Flexible Nanoporous Tunable Electrical Double Layer Biosensors For Sweat Diagnostics
Rujuta D. Munje, Sriram Muthukumar, Anjan Panneer Selvam, Shalini Prasad
Published 2015 · Medicine, Materials Science
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An ultra-sensitive and highly specific electrical double layer (EDL) modulated biosensor, using nanoporous flexible substrates for wearable diagnostics is demonstrated with the detection of the stress biomarker cortisol in synthetic and human sweat. Zinc oxide thin film was used as active region in contact with the liquid i.e. synthetic and human sweat containing the biomolecules. Cortisol detection in sweat was accomplished by measuring and quantifying impedance changes due to modulation of the double layer capacitance within the electrical double layer through the application of a low orthogonally directed alternating current (AC) electric field. The EDL formed at the liquid-semiconductor interface was amplified in the presence of the nanoporous flexible substrate allowing for measuring the changes in the alternating current impedance signal due to the antibody-hormone interactions at diagnostically relevant concentrations. High sensitivity of detection of 1 pg/mL or 2.75 pmol cortisol in synthetic sweat and 1 ng/mL in human sweat is demonstrated with these novel biosensors. Specificity in synthetic sweat was demonstrated using a cytokine IL-1β. Cortisol detection in human sweat was demonstrated over a concentration range from 10–200 ng/mL.
This paper references
Organic field-effect transistor-based biosensors functionalized with protein receptors
Francesco Maddalena (2010)
Nanosensor electrical immunoassay for quantitative detection of NT-pro brain natriuretic peptide.
Anjan Panneer Selvam (2013)
Functionalizing Zn- and O-terminated ZnO with thiols
Patrick W. Sadik (2007)
Recent advances in cortisol sensing technologies for point-of-care application.
Ajeet Kaushik (2014)
Network single-walled carbon nanotube-field effect transistors (SWNT-FETs) with increased Schottky contact area for highly sensitive biosensor applications.
Hye Ryung Byon (2006)
Electrochemical l-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase
Zafar Hussain Ibupoto (2012)
Optimised pulsed laser deposition of ZnO thin films on transparent conducting substrates
Joseph B. Franklin (2011)
Single molecule unfolding and stretching of protein domains inside a solid-state nanopore by electric field
Kevin J. Freedman (2013)
Unfolding of proteins and long transient conformations detected by single nanopore recording.
Ghani Oukhaled (2007)
High-Density Carrier Accumulation in ZnO Field-Effect Transistors Gated by Electric Double Layers of Ionic Liquids
Hongtao Yuan (2009)
TiCxOy thin films for decorative applications: Tribocorrosion mechanisms and synergism
Mathew T. Mathew (2008)
DOi: 10.1038/srep14586 Author Contributions R.D.M. ran the experiments, carried out data analysis and co-wrote the paper
Increasing Energy Storage in Electrochemical Capacitors with Ionic Liquid Electrolytes and Nanostructured Carbon Electrodes
J. Vatamanu (2013)
Interference testing in clinical chemistry; approved guideline
R. J. McEnroe (2005)
Acknowledgments We sincerely thank Dr. Manuel Quevedo-Lopez (Department of Materials Science and Engineering, UT Dallas) for the equipment related support
Electrochemical analysis based on nanoporous structures.
Sangyun Park (2012)
Structural and morphological properties of thin ZnO films grown by pulsed laser deposition
Mirela Petruta Suchea (2008)
Label-free, chemiresistor immunosensor for stress biomarker cortisol in saliva.
Chaker Tlili (2011)
Zinc oxide nanostructures: from growth to application
Jorge L. Gomez (2012)
Programming the detection limits of biosensors through controlled nanostructuring.
Leyla Soleymani (2009)
Method comparison and bias estimation using patient samples ; approved guideline
J. S. Krouwer (2002)
Method comparison and bias estimation using patient samples; approved guideline. NCCLS document EP9-A2 (ISBN 1-56238-472-4)
The dissolved constituents of human sweat.
A. G. R. Whitehouse (1935)
Accumulation mode field-effect transistors for improved sensitivity in nanowire-based biosensors
David J. Baek (2012)
Label-free protein biosensor based on aptamer-modified carbon nanotube field-effect transistors.
Kenzo Maehashi (2007)
Ultra-sensitive electrical immunoassay biosensors using nanotextured zinc oxide thin films on printed circuit board platforms.
Michael P. Jacobs (2014)
MoS₂ field-effect transistor for next-generation label-free biosensors.
Deblina Sarkar (2014)
A Low-Voltage Low-Power Front-End for Wearable EEG Systems
D. C. Yates (2007)
from growth to application
J. L. Gomez (2013)
OPTICAL PROPERTIES OF PULSED LASER DEPOSITED ZnO THIN FILMS
Weisheng Hu (1997)
CMOS-compatible, label-free silicon-nanowire biosensors to detect cardiac troponin I for acute myocardial infarction diagnosis.
Tao Kong (2012)
Limit of blank, limit of detection and limit of quantitation.
David A. Armbruster (2008)
Direct Growth of Vertically-oriented Graphene for Field-Effect Transistor Biosensor
Shun Mao (2013)
This paper is referenced by
SMART Biosensor for Early Diagnostic Detection of Metal Ion Release in Orthopedic Patients: Initial Outcome
Mathew T. Mathew (2018)
Flex-GO (Flexible graphene oxide) sensor for electrochemical monitoring lactate in low-volume passive perspired human sweat.
Kai-Chun Lin (2020)
Wearable sweat sensors
Mallika Bariya (2018)
Portable impedance measurement device for sweat based glucose detection
Athul Asokan Thulasi (2017)
Sensors Based on Bio and Biomimetic Receptors in Medical Diagnostic, Environment, and Food Analysis
Alisa N. Kozitsina (2018)
The design, fabrication, and applications of flexible biosensing devices.
Meng Xu (2019)
Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection
Lu Cao (2018)
Development of ultra-low volume, multi-bio fluid, cortisol sensing platform
Sayali Upasham (2018)
Flexible Molybdenum Electrodes towards Designing Affinity Based Protein Biosensors
Vikramshankar Kamakoti (2016)
Comparison of Colorimetric Analyses to Determine Cortisol in Human Sweat
Ethan Tu (2020)
Energy Autonomous Wearable Sensors for Smart Healthcare: A Review
Abhishek Singh Dahiya (2019)
AWARE: A Wearable Awareness with Real-time Exposure, for monitoring alcohol consumption impact through Ethyl Glucuronide detection.
David Kinnamon (2018)
Chapter Three - New Trends in Antibody-Based Electrochemical Biosensors
Mamas I. Prodromidis (2017)
Label-free detection of allergens in food via surfactant-induced signal amplification using a field effect transistor-based biosensor
Sho Hideshima (2018)
Simultaneous lancet-free monitoring of alcohol and glucose from low-volumes of perspired human sweat
Ashlesha Bhide (2018)
Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor
Reza Hajian (2019)
Salivary Cortisol Determination on Smartphone-Based Differential Pulse Voltammetry System
Jingjing Liu (2020)
Predicting limits of detection in real-time sweat-based human performance monitoring
Melanie Rudolph (2019)
Highly sensitive and non-invasive electrochemical immunosensor for salivary cortisol detection
Nidhi Dhull (2019)
Stress Biomarkers in Biological Fluids and Their Point-of-Use Detection.
Andrew J. Steckl (2018)
Non-invasive monitoring of a circadian relevant biomarker from easily accessible body fluids using hybrid aqueous–ionic buffer interfaces on flexible substrates
Sayali Upasham (2019)
Screen Printed Graphene Oxide Textile Biosensor for Applications in Inexpensive and Wearable Point-of-Exposure Detection of Influenza for At-Risk Populations
David Kinnamon (2018)
Biomarkers in Health Care
Adama Marie Sesay (2017)
Portable biosensor for monitoring cortisol in low-volume perspired human sweat
David Kinnamon (2017)
Flexible plastic, paper and textile lab-on-a chip platforms for electrochemical biosensing.
Anastasios Economou (2018)
Electrical double layer modulation of hybrid room temperature ionic liquid/aqueous buffer interface for enhanced sweat based biosensing.
Badrinath Jagannath (2018)
Fully electronic urine dipstick probe for combinatorial detection of inflammatory biomarkers
Vikramshankar Kamakoti (2018)
Flexible Electrochemical Bioelectronics: The Rise of In Situ Bioanalysis.
You Yu (2019)
Electronic Skin: Recent Progress and Future Prospects for Skin-Attachable Devices for Health Monitoring, Robotics, and Prosthetics.
Jun Yang (2019)
A Four-Channel Electrical Impedance Spectroscopy Module for Cortisol Biosensing in Sweat-Based Wearable Applications
Devangsingh Sankhala (2018)
CMOS Ion Sensitive Field Effect Transistors for Highly Sensitive Detection of DNA Hybridization
Chieh-Feng Chang (2020)
Carbon fiber based electrochemical sensor for sweat cortisol measurement
Michael Sekar (2019)See more