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Oriented Immobilization Of Antibodies And Its Applications In Immunoassays And Immunosensors.

B. Lu, M. Smyth, R. O'Kennedy
Published 1996 · Chemistry, Medicine

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The immobilization of antibodies on solid-phase materials has been used in many areas such as purification, diagnostic immunoassays and immunosensors. Problems associated with the loss of biological activity of the antibodies upon immobilization have been noticed in many cases. One of the main reasons for such loss is attributed to the random orientation of the asymmetric macromolecules on support surfaces. In this paper, the approaches for achieving oriented coupling of antibodies to increase the antigen binding capacity are reviewed. Some issues such as steric hindrance caused by neighbouring antibody molecules, the distance between an antibody and the support surface and the use of antibody fragments are dealt with. Some applications of the oriented immobilized antibodies in immunoassays and immunosensors are examined.
This paper references
10.1016/0022-1759(78)90131-X
[125I]protein A: a tracer for general use in immunoassay.
J. Langone (1978)
10.1016/0022-1759(89)90079-3
The influence of adsorption of native and modified antibodies on their activity.
J. N. Lin (1989)
10.1016/S0021-9673(01)93766-5
Enzymic oxidation of monoclonal antibodies by soluble and immobilized bifunctional enzyme complexes.
B. Solomon (1990)
10.1016/S0003-2670(00)80664-9
Fiber-optic-based immunosensors for haptens
T. Betts (1991)
10.1016/0022-1759(88)90041-5
Site-specific immobilization of antibodies by their oligosaccharide moieties to new hydrazide derivatized solid supports.
W. Hoffman (1988)
10.1016/0003-2670(93)85334-G
Detection of human T-lymphocytes with a piezoelectric immunosensor
B. König (1993)
10.1016/S0003-2670(00)85125-9
Evaluation of antibody immobilization technqiues for fiber optic-based fluoroimmunosensing
J. Alarie (1990)
10.1021/AC00075A005
A novel immunosensor for herpes viruses.
B. König (1994)
10.1016/0003-2670(94)80050-2
Heterogeneous fluorescence immunoassays using flow-injection analysis with protein G solid phase reactors
M. Khokhar (1994)
10.1038/366469A0
Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack
P. Tavladoraki (1993)
10.1016/0141-0229(93)90166-Y
Random and site-specific immobilization of catalytic antibodies.
T. M. Spitznagel (1993)
10.1021/AC00209A019
Regenerable fiber-optic-based immunosensor.
F. Bright (1990)
10.1111/j.1749-6632.1990.tb18184.x
The Specific Immobilization of Antibody Fragments on Membrane for the Development of Multifunctional Biosensors
J. L. Boitieux (1990)
10.1042/BJ2900723
Expression, purification and characterization of B72.3 Fv fragments.
D. J. King (1993)
10.1038/NBT0994-899
Guiding the Selection of Human Antibodies from Phage Display Repertoires to a Single Epitope of an Antigen
L. S. Jespers (1994)
Determinants of specificity of a baculovirus-expressed antibody Fab fragment that binds selectively to the activated form of integrin alpha IIb beta 3.
C. Abrams (1994)
10.1016/S0021-9673(00)90554-5
Strategy for the immobilization of monoclonal antibodies on solid-phase supports.
R. S. Matson (1988)
10.1021/AC00097A014
Oriented Immobilization of Fab' Fragments on Silica Surfaces
B. Lu (1995)
10.1016/S0003-2670(00)84617-6
Enzyme immunoelectrode for insulin incorporating a membrane partially treated with water vapour plasma
Shigeki Kaku (1989)
10.1021/AC00105A021
Protein A Langmuir-Blodgett Film for Antibody Immobilization and Its Use in Optical Immunosensing
K. Owaku (1995)
10.1039/AN9941900943
Rapid fluorescence flow injection immunoassay using a novel perfusion chromatographic material
D. Palmer (1994)
10.1038/NBT0793-825
Surface-Density and Orientation Effects on Immobilized Antibodies and Antibody Fragments
T. M. Spitznagel (1993)
10.1080/00032719308021481
Detection of viruses and bacteria with piezoelectric immunosensors
B. König (1993)
10.1021/BI00458A002
A comparison of strategies to stabilize immunoglobulin Fv-fragments.
R. Glockshuber (1990)
10.1039/AN9921701679
Flow injection electrochemical enzyme immunoassay for theophylline using a protein A immunoreactor and p-aminophenyl phosphate-p-aminophenol as the detection system.
D. Palmer (1992)
10.1016/0022-1759(78)90133-3
A rapid, novel method for the solid-phase derivatization of IgG antibodies for immune-affinity chromatography.
D. Gersten (1978)
Differential IgG-binding characteristics of staphylococcal protein A, streptococcal protein G, and a chimeric protein AG.
M. Eliasson (1989)
10.1016/S0021-9673(01)88747-1
Comparison of site-specific coupling chemistry for antibody immobilization on different solid supports
Lin Jinn-Nan (1991)
10.1016/0022-0728(89)85159-9
Alkaline phosphatase as a label for a heterogeneous immunoelectrochemical sensor: An electrochemical study
I. Rosen (1989)
10.1016/0022-2836(88)90214-8
Expression of an antibody Fv fragment in myeloma cells.
L. Riechmann (1988)
10.1002/BIT.260281007
Adsorption equilibrium in immuno‐affinity chromatography with polyclonal and monoclonal antibodies
Eizo Sada (1986)
10.1016/0022-1759(90)90071-3
An improved method for immobilizing IgG antibodies on protein A-agarose.
T. Sisson (1990)
Chimeric IgG-binding receptors engineered from staphylococcal protein A and streptococcal protein G.
M. Eliasson (1988)
A one-step purification of membrane proteins using a high efficiency immunomatrix.
C. Schneider (1982)
Evaluation of CNBr, FMP and hydrazide resins for immunoaffinity purification of factor IX.
F. Highsmith (1992)
10.1016/0003-2697(92)90440-I
Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor.
R. A. Ogert (1992)



This paper is referenced by
10.1201/9780203911129.CH3
Practical Approaches to Protein Microarrays
Brian B. Haab (2003)
10.1039/C4TB01825G
Thromboresistant and rapid-endothelialization effects of dopamine and staphylococcal protein A mediated anti-CD34 coating on 316L stainless steel for cardiovascular devices.
Jialong Chen (2015)
10.1016/J.SNB.2010.03.039
SPR biosensor by using E. coli outer membrane layer with autodisplayed Z-domains
Min Park (2011)
10.1007/s11814-013-0117-5
A comparative study on antibody immobilization strategies onto solid surface
Ji Eun Lee (2013)
10.1002/anie.201706765
Docking of Antibodies into the Cavities of DNA Origami Structures.
X. Ouyang (2017)
10.1016/j.bmcl.2016.12.025
Recent advances on the encoding and selection methods of DNA-encoded chemical library.
B. Shi (2017)
10.1016/J.CHROMA.2007.05.023
Improving immunobinding using oriented immobilization of an oxidized antibody.
J. H. Kang (2007)
10.1039/C6AN02145J
Antibody purification via affinity membrane chromatography method utilizing nucleotide binding site targeting with a small molecule.
N. Mustafaoglu (2016)
10.1016/J.MSEC.2010.10.015
Densities and orientations of antibodies on nano-textured silicon surfaces
Satyendra Kumar (2011)
10.1201/B14900-16
Non-covalent immobilization of proteins to surfaces
Pascal Jonkheijm (2013)
10.1371/journal.pone.0112070
Bi-Epitope SPR Surfaces: A Solution to Develop Robust Immunoassays
L. Peng (2014)
10.1016/J.SNB.2005.03.018
Site-directed antibody immobilization on gold substrate for surface plasmon resonance sensors
A. H. Schmid (2006)
10.1016/j.tibtech.2011.07.002
Creating permissive microenvironments for stem cell transplantation into the central nervous system.
H. Kim (2012)
10.1016/j.addr.2017.07.011
Strategies to develop endogenous stem cell‐recruiting bioactive materials for tissue repair and regeneration
Settimio Pacelli (2017)
10.1016/J.BIOS.2005.07.002
A micro-immuno supported liquid membrane assay (mu-ISLMA).
M. Tudorache (2006)
10.1021/ac400949t
Oriented covalent immobilization of antibodies for measurement of intermolecular binding forces between zipper-like contact surfaces of split inteins.
Mirco Sorci (2013)
10.1007/978-0-387-98063-8_10
A Fast and Sensitive Integrated Young Interferometer Biosensor
Johannes S. Kanger (2009)
10.1016/S0009-8981(01)00629-5
Immunosensors--principles and applications to clinical chemistry.
P. Luppa (2001)
10.1016/J.CHROMA.2007.03.032
Preparation of novel immunomagnetic cellulose microspheres via cellulose binding domain-protein A linkage and its use for the isolation of interferon alpha-2b.
Yu Cao (2007)
10.1039/C5AY01651G
Development of a reusable protein G based SPR immunosensor for direct human growth hormone detection in real samples
A. Makaraviciute (2015)
10.1016/j.talanta.2017.08.017
Chain-length dependent interfacial immunoreaction kinetics on self-assembled monolayers revealed by surface-enhanced infrared absorption spectroscopy.
Wen-Jing Bao (2018)
Bio-nanocapsules for oriented immobilization of biological sensing molecules
飯嶋 益巳 (2011)
10.1039/c7cc02057k
Improving bioassay sensitivity through immobilization of bio-probes onto reactive micelles.
Gloria Jiménez-Sánchez (2017)
10.1364/OL.30.001021
Miniature fiber-optic multicavity Fabry-Perot interferometric biosensor.
Y. Zhang (2005)
10.1016/J.CCCN.2004.01.009
Advances in recombinant antibody microarrays.
P. Pavlickova (2004)
10.1016/j.ab.2011.10.036
Effect of antibody modifications on its biomolecular binding as determined by surface plasmon resonance.
S. K. Vashist (2012)
10.1002/JSSC.200600062
A comparative evaluation of random and site-specific immobilization techniques for the preparation of antibody-based chiral stationary phases.
E. J. Franco (2006)
Structured antibody surfaces for bio-recognition and a label-free detection of bacteria
C. Wolf (2010)
10.1016/0003-2670(96)00191-2
Immunological activities of IgG antibody on pre-coated Fc receptor surfaces
B. Lu (1996)
10.1016/J.SUSC.2007.08.018
Immobilization of protein molecules on step-controlled sapphire surfaces
Rie Aoki (2007)
10.32657/10356/43311
Studies of surface sciences of protein immobilizations for novel immunosensors.
Weihua Hu (2011)
10.1016/B978-0-12-381373-2.00071-5
3.07 – Clinical Immunoassays and Immunosensing
Jifeng Wu (2012)
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