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Effect Of Crystallinity Of Calcium Phosphate Nanoparticles On Adhesion, Proliferation, And Differentiation Of Bone Marrow Mesenchymal Stem Cells
Q. Hu, Zhou Tan, Y. Liu, Jinhui Tao, Yu-rong Cai, M. Zhang, Haihua Pan, Xurong Xu, R. Tang
Published 2007 · Materials Science
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The biomedical materials research community frequently accepts that amorphous calcium phosphate (ACP) can be adsorbed and assimilated more readily by living organisms to produce new bone tissue than crystallized calcium phosphates such as hydroxyapatite (HAP). Previous studies also confirm that ACP has improved bioactivity compared to HAP since more adhesion and proliferation of osteogenic cells are observed on the ACP substrates. However, we note that the different size -effects of calcium phosphates are not taken into account in these studies and the used ACP are always smaller than the HAP. Our recent study reveals that the dimensions of nanoparticles are directly related to the bioactivities of calcium phosphates, e.g. the smaller nanocrystallites have a greater promotion effect on the proliferation of bone marrow mesenchymal stem cells (BMSCs). In order to understand the influence of crystallinity of calcium phosphate on the osteogenic cells correctly, it is critical to use ACP and HAP nanoparticles which have the same size distribution in such comparisons. In the present work, ∼20 nm ACP and HAP particles are synthesized and the effects of crystallinity of calcium phosphates are studied. The adhesion, proliferation, and differentiation of BMSCs are measured on ACP and HAP films, which are compared at the same size scale. It is surprising that more cells adsorb and proliferate on the film of well crystallized HAP than those on the ACP film. Alkaline phosphatase (ALP) activity assay and reverse transcription-polymerase chain reaction (RT-PCR) assay are also used to evaluate the differentiation of BMSCs. The results show that the differentiation of BMSCs to osteoblasts is promoted significantly by NanoHAP. The current experimental phenomena clearly demonstrate that the crystallized phase of calcium phosphate, HAP, provides a better substrate for BMSCs than the amorphous one, ACP, when the factor of size effect is removed. A new view on the relationship between the crystallinity of calcium phosphate and the responses of BMSCs indicates the importance of size and phase controls in the application of biomedical materials.
This paper references
A thermodynamic analysis of the secondary transition in the spontaneous precipitation of calcium phosphate
J. Meyer (2005)
Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses.
K. Anselme (2000)
The structure of bone studied with synchrotron X-ray diffraction, X-ray absorption spectroscopy and thermal analysis
F. Peters (2000)
Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants
W. Suchanek (1998)
Nanocrystalline calcium phosphate ceramics in biomedical engineering
S. Kalita (2007)
Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD.
G. Balasundaram (2006)
Amplification of human beta-actin gene by the reverse transcriptase-polymerase chain reaction: implications for assessment of RNA from formalin-fixed, paraffin-embedded material.
A. Dakhama (1996)
Structural studies of a phosphatidyl serine-amorphous calcium phosphate complex
M. Taylor (1998)
Expression of bone matrix proteins associated with mineralized tissue formation by adult rat bone marrow cells in vitro: Inductive effects of dexamethasone on the osteoblastic phenotype
S. Kasugai (1991)
Autologous mesenchymal stem cell-mediated repair of tendon.
H. Awad (1999)
Characterization of the interface in the plasma-sprayed HA coating/Ti-6Al-4V implant system.
M. Filiaggi (1991)
Comparative biological properties of HA plasma-sprayed coatings having different crystallinities
P. Frayssinet (1994)
Osseointegration of macroporous calcium phosphate ceramics having a different chemical composition.
P. Frayssinet (1993)
Functionally graded tricalcium phosphate/fluoroapatite composites
L. Wong (2002)
In vitro precipitation of calcium phosphate under intracellular conditions: formation of brushite from an amorphous precursor in the absence of ATP
Roy E. Wuthier (2007)
Differences of bone bonding ability and degradation behaviour in vivo between amorphous calcium phosphate and highly crystalline hydroxyapatite coating.
M. Nagano (1996)
IN VITRO MINERALIZATION AND IMPLANT CALCIUM PHOSPHATE-HYDROXYAPATITE CRYSTALLINITY
J. Morgan (1996)
Wet synthesis and characterization of modified hydroxyapatite powders
R. Correia (1996)
Conversion of amorphous calcium phosphate into hydroxyapatite investigated by EXAFS spectroscopy
J. Harries (1987)
Osteotransductive bone cements
F. Driessens (1998)
Calcium phosphate ceramics as hard tissue prosthetics.
M. Jarcho (1981)
Role of hydroxyapatite nanoparticle size in bone cell proliferation
Yu-rong Cai (2007)
Analysis of the EXAFS spectrum of hydroxyapatite
J. Harries (1986)
Mineralisation of chitosan scaffolds with nano-apatite formation by double diffusion technique.
I. Manjubala (2006)
Formation of hydroxyapatite in new calcium phosphate cements.
S. Takagi (1998)
Osteoinduction in porous hydroxyapatite implanted in heterotopic sites of different animal models.
U. Ripamonti (1996)
Osteogenesis in extraskeletally implanted porous calcium phosphate ceramics: variability among different kinds of animals.
Z. Yang (1996)
Biologically and chemically optimized composites of carbonated apatite and polyglycolide as bone substitution materials.
W. Linhart (2001)
Relationship between collagen synthesis and expression of the osteoblast phenotype in MC3T3‐E1 cells
R. Franceschi (1992)
Effects of surface undulations of biphasic calcium phosphate tablets on human osteoblast behavior.
E. A. dos Santos (2005)
A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics.
H. Yuan (1999)
Calcium phosphates in oral biology and medicine.
R. Legeros (1991)
Cell-extracellular matrix interaction and physico-chemical characteristics of titanium surfaces depend on the roughness of the material.
R. Lange (2002)
Mechanical and chemical consequences of the residual stresses in plasma sprayed hydroxyapatite coatings.
V. Sergo (1997)
Effects of hydroxylapatite coating crystallinity on biosolubility, cell attachment efficiency and proliferation in vitro.
L. Chou (1999)
Causes of phosphate stone formation and the importance of metaphylaxis by urinary acidification: a review
A. Hesse (1999)
Continuous synthesis of amorphous carbonated apatites.
D. Tadic (2002)
Determination of contact angles and pore sizes of porous media by column and thin layer wicking
C. V. Oss (1992)
Mineralization and the Expression of Matrix Proteins During In Vivo Bone Development
E. A. Cowles (1998)
In vivo study of calcium phosphate cements: implantation of an alpha-tricalcium phosphate/dicalcium phosphate dibasic/tetracalcium phosphate monoxide cement paste.
K. Kurashina (1997)
KONTINUIERLICHE HERSTELLUNG VON KNOCHENMINERALÄHNLICHEN CALCIUMPHOSPHAT -KERAMIKEN
Dusanka Tadic (2001)
Structural arrangements at the interface between plasma sprayed calcium phosphates and bone.
J. D. de Bruijn (1994)
Human osteoclasts, not osteoblasts, deposit osteopontin onto resorption surfaces: An in vitro and ex vivo study of remodeling bone
Robert A. Dodds (1995)
An electron microscopic study of the formation of amorphous calcium phosphate and its transformation to crystalline apatite
E. Eanes (2005)
Mesenchymal stem cells used for rabbit tendon repair can form ectopic bone and express alkaline phosphatase activity in constructs
M. T. Harris (2004)
Expression of Bone Matrix Proteins mRNA During Distraction Osteogenesis
M. Sato (1998)
Bioceramics: From Concept to Clinic
L. Hench (1991)
Comparative chemistry of amorphous and apatitic calcium phosphate preparations
J. Termine (2005)
Differences in intracortical bone remodeling in three aboriginal American populations: Possible dietary factors
E. A. Richman (2006)
Osteoblastic differentiation of cultured rat bone marrow cells on hydroxyapatite with different surface topography.
A. L. Rosa (2003)
Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength.
D. Deligianni (2001)
Bone cell behavior on Matrigel-coated Ca/P coatings of varying crystallinities.
S. Maxian (1998)
A thermodynamic analysis of the amorphous to crystalline calcium phosphate transformation
J. Meyer (2005)
This paper is referenced by
Preparation, characterization and in vitro osteoblast growth of waste-derived biomaterials
E. S. Rojo (2014)
Surface functionalization of halloysite nanotubes with supermagnetic iron oxide, chitosan and 2-D calcium-phosphate nanoflakes for synergistic osteoconduction enhancement of human adipose tissue-derived mesenchymal stem cells.
Y. Lee (2019)
Development Of In Vitro Models To Examine Cell-Mineral Interaction And Cell-Mediated Mineral Formation
D. Lin (2013)
Phosphorylated dendronized poly(amido amine)s as protein analogues for directing hydroxylapatite biomineralization.
Jianyu Xin (2014)
Strontium eluting graphene hybrid nanoparticles augment osteogenesis in a 3D tissue scaffold.
S. Kumar (2015)
Structure of polymer and particle aggregates in hydrogel composites
Ida Berts (2013)
Facile fabrication of bioactive ultra-small protein-hydroxyapatite nanoconjugates via liquid-phase laser ablation and their enhanced osteogenic differentiation activity.
M. Rodio (2017)
Nano-hydroxyapatite/polyacrylamide composite hydrogels with high mechanical strengths and cell adhesion properties.
Z. Li (2014)
The role played by modified bioinspired surfaces in interfacial properties of biomaterials
T. T. Paterlini (2017)
New Nanocomposite System with Nanocrystalline Apatite Embedded into Mesoporous Bioactive Glass
M. Cicuéndez (2012)
In vitro osteoblast-like cell proliferation on nano-hydroxyapatite coatings with different morphologies on a titanium-niobium shape memory alloy.
J. Xiong (2010)
Effect of Roughness on in Situ Biomineralized CaP-Collagen Coating on the Osteogenesis of Mesenchymal Stem Cells.
X. Zan (2016)
Surface Modification of Hydroxyapatite Nanocrystallite by a Small Amount of Terbium Provides a Biocompatible Fluorescent Probe
L. Li (2008)
Calcium Phosphate Biomaterials for Bone Tissue Engineering: Properties and Relevance in Bone Repair
K. Maji (2020)
Radio Frequency Magnetron Sputter Deposition as a Tool for Surface Modification of Medical Implants
R. Surmenev (2017)
Role of fetal bovine serum in the prevention of calcification in biological fluids
P. Liu (2008)
Towards understanding biomineralization: calcium phosphate in a biomimetic mineralization process
Yu-rong Cai (2009)
Structure and properties of hydroxyapatite for biomedical applications
K. Lin (2015)
Cellular shellization: Surface engineering gives cells an exterior
B. Wang (2010)
The effect of pre-coating human bone marrow stromal cells with hydroxyapatite/amino acid nanoconjugates on osteogenesis.
Jodie C. Babister (2009)
Dextran-coated fluorapatite nanorods doped with lanthanides in labelling and directing osteogenic differentiation of bone marrow mesenchymal stem cells.
H. Zeng (2014)
Emerging translational research on magnetic nanoparticles for regenerative medicine.
Y. Gao (2015)
Biomineralization of a self-assembled extracellular matrix for bone tissue engineering.
Y. Meng (2009)
Biomimetic calcium-silicate cements aged in simulated body solutions. Osteoblast response and analyses of apatite coating.
M. Gandolfi (2009)
The importance of being amorphous: calcium and magnesium phosphates in the human body.
Rita Gelli (2019)
Advanced biocomposites of poly(glycerol sebacate) and β-tricalcium phosphate by in situ microwave synthesis for bioapplication
C. C. Lau (2020)
Nanosized and nanocrystalline calcium orthophosphates.
S. Dorozhkin (2010)
Biomimetic Calcium-Silicate Cements Aged in Simulated Body Solutions. Osteoblast Response and Analyses of Apatite Coating
M. Gandolfi (2009)
Design of Nanocomposite Materials Using Molecular Modeling
Jonáš Tokarský (2010)
Chemical and physical properties of regenerative medicine materials controlling stem cell fate
E. Kaivosoja (2012)
Differential effect of hydroxyapatite nano-particle versus nano-rod decorated titanium micro-surface on osseointegration.
Long Bai (2018)
The formulation of novel calcium phosphate containing culture beads for cell therapy
Parastoo Jamshidi (2015)See more