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Detection Of Chymase Activity Using A Specific Peptide Probe Conjugated Onto Gold Nanoparticles

Hui-Fang Chang, Hui-Fang Chang, Y. Sun, Fang-Yuan Yeh, I-Hua Tseng, Chia-Chu Chang, Chih-Sheng Lin
Published 2018 · Chemistry

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Gold nanoparticles (AuNPs) can be applied in biosensors using fluorescence resonance energy transfer (FRET) technique. Based on this technique, we have established a sensitive and efficient biosensing method by modifying a peptide-probe onto AuNPs to detect proteinase enzyme activity in this study. This biosensing method was designed for chymase activity detection and applied in kidney disease diagnosis. In this study, 16 nm-AuNPs were used to construct the AuNPs-based fluorescence peptide probe (named AuNPs-peptide probe) for chymase activity determination. The peptide sequence is FITC-Acp-DRVYIHPFHLDDDDDC, which comprises a fluorophore at the N-terminal end, an enzyme (chymase) substrate (DRVYIHPFHL), a spacer (DDDDD) and cysteine (C) to conjugate to AuNPs surface. When the enzyme catalyzes the substrate sequence, the fluorophore drifts away from AuNPs and the fluorescence emitting signal can be excited at 495 nm and detected at 515 nm. The results indicate that the time required for the AuNPs-peptide probe for activity detection of chymase was only 15 min, and a linear correlation from 10 to 100 ng mL−1 of chymase was acquired. The chymase reaction would be significantly inhibited by addition of specific chymase inhibitor chymostatin. The AuNPs-peptide probe was tested for the detection of high concentrations of trypsin and chymotrypsin, but only minor emitted fluorescence intensity was detected. According to these results, sensitivity and specificity of the AuNPs-peptide probe for chymase detection have been confirmed. AuNPs-peptide probe was successfully used for the detection of renal chymase activity; and the results indicate the pathogenically increased chymase activity in kidney tissue of nephropathic mice from aristolochic acid I treatment.
This paper references
10.1038/hr.2009.205
Homocysteine-induced oxidative stress upregulates chymase in mouse mastocytoma cells
K. Fujimi (2010)
10.1016/S0022-5223(03)00697-4
Attenuation of adhesion formation after cardiac surgery with a chymase inhibitor in a hamster model.
Y. Soga (2004)
10.3109/1040841X.2013.879849
Metal nanoparticles: The protective nanoshield against virus infection
M. Rai (2016)
10.1039/C5RA05350A
Coumarin-modified gold nanoprobes for the sensitive detection of caspase-3
Q. Wang (2015)
10.1021/bc8003028
A multidentate peptide for stabilization and facile bioconjugation of gold nanoparticles.
Zeljka Krpetić (2009)
10.1021/JM0700619
Discovery of potent, selective, orally active, nonpeptide inhibitors of human mast cell chymase.
M. N. Greco (2007)
10.1021/JP405265U
Colloidal Stability of Gold Nanoparticles Coated with Multithiol-Poly(ethylene glycol) Ligands: Importance of Structural Constraints of the Sulfur Anchoring Groups
E. Oh (2013)
10.7150/ijbs.13379
Alternative Roles of STAT3 and MAPK Signaling Pathways in the MMPs Activation and Progression of Lung Injury Induced by Cigarette Smoke Exposure in ACE2 Knockout Mice
Yi-Han Hung (2016)
10.1258/ebm.2012.011356
Role of chymase in diabetic nephropathy
P. Cristovam (2012)
10.1109/TNB.2008.2011733
Optical Detection of Human Papillomavirus Type 16 and Type 18 by Sequence Sandwich Hybridization With Oligonucleotide-Functionalized Au Nanoparticles
Sz-Hau Chen (2009)
10.1039/c1cs15280g
Gold nanoparticle-enabled biological and chemical detection and analysis.
H. Jans (2012)
10.1161/HYPERTENSIONAHA.111.202424
Direct Evidence for Intrarenal Chymase-Dependent Angiotensin II Formation on the Diabetic Renal Microvasculature
S. Park (2013)
10.1007/S11468-007-9033-Z
Tunable Assembly of Peptide-coated Gold Nanoparticles
J. Tullman (2007)
10.1021/NL0480969
Gold nanoparticles quench fluorescence by phase induced radiative rate suppression.
E. Dulkeith (2005)
10.1016/j.bios.2014.05.007
Gold nanoparticles conjugates-amplified aptamer immunosensing screen-printed carbon electrode strips for thrombin detection.
Fang-Yuan Yeh (2014)
10.1021/ja711330f
Folding induced assembly of polypeptide decorated gold nanoparticles.
D. Aili (2008)
10.3109/1040841X.2015.1018131
Strategic role of selected noble metal nanoparticles in medicine
M. Rai (2016)
10.3906/KIM-1311-6
Linear assembly and 3D networks of peptide modified gold nanoparticles
Şaban Kalay (2014)
10.1039/C3RA46493H
A general colorimetric method for detecting protease activity based on peptide-induced gold nanoparticle aggregation
Ganchao Chen (2014)
10.1016/j.bios.2008.02.030
Disposable amperometric immunosensing strips fabricated by Au nanoparticles-modified screen-printed carbon electrodes for the detection of foodborne pathogen Escherichia coli O157:H7.
Y. Lin (2008)
10.1039/C4RA00258J
Spacer-enhanced chymotrypsin-activated peptide-functionalized gold nanoparticle probes: a rapid assay for the diagnosis of pancreatitis
Fang-Yuan Yeh (2014)
10.1021/BI00054A021
Quantitative analysis of liposome-cell interactions in vitro: rate constants of binding and endocytosis with suspension and adherent J774 cells and human monocytes.
K. Lee (1993)
10.1002/biot.201300201
FRET‐based and other fluorescent proteinase probes
H. Hu (2014)
10.1097/01.ASN.0000071512.93927.4E
Chymase is upregulated in diabetic nephropathy: implications for an alternative pathway of angiotensin II-mediated diabetic renal and vascular disease.
X. Huang (2003)
10.1007/s00216-012-6418-4
Quantifying dithiothreitol displacement of functional ligands from gold nanoparticles
De-Hao Tsai (2012)
10.1161/CIRCRESAHA.117.310978
Multifunctional Role of Chymase in Acute and Chronic Tissue Injury and Remodeling.
L. Dell'Italia (2018)
10.2174/157340212800505007
Impact of Mast Cell Chymase on Renal Disease Progression.
H. Wasse (2012)
10.2174/1389450115666140804124808
Safety of Nanoparticles in Medicine.
Joy Wolfram (2015)
10.1016/J.SNB.2018.06.021
A sensitive ratiometric fluorescence probe for chymotrypsin activity and inhibitor screening
Y. Chen (2018)
10.1016/j.bios.2014.09.059
A fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of mecA gene sequence of Staphylococcus aureus.
J. Shi (2015)
10.1291/hypres.31.1517
Evidence for Abundant Presence of Chymase-Positive Mast Cells in the Kidneys of Patients with Immunoglobulin A Nephropathy: Effect of Combination Therapy with Prednisolone and Angiotensin II Receptor Blocker Valsartan
Y. Konishi (2008)
10.1016/j.aca.2018.01.053
A label-free fluorescent biosensor for the detection of protein kinase activity based on gold nanoclusters/graphene oxide hybrid materials.
Q. Liu (2018)
10.1016/j.bios.2011.11.002
Aqueous zymography screening of matrix metalloproteinase activity and inhibition based on colorimetric gold nanoparticles.
Yao-Chen Chuang (2012)
10.1016/j.bios.2016.08.029
Label-free fluorometric detection of chymotrypsin activity using graphene oxide/nucleic-acid-stabilized silver nanoclusters hybrid materials.
Shuangqin Li (2017)
10.1159/000317102
Mast Cell Infiltration Is Involved in Renal Interstitial Fibrosis in a Rat Model of Protein-Overload Nephropathy
Y. Li (2010)
10.1016/0079-6816(93)90013-L
Theory of the stability of strongly charged lyophobic sols and of the adhesion of strongly charged particles in solutions of electrolytes
B. Derjaguin (1941)
10.1016/j.biomaterials.2010.04.026
An optical biosensing platform for proteinase activity using gold nanoparticles.
Yao-Chen Chuang (2010)
10.1016/j.bios.2016.01.041
Biosensors based on modularly designed synthetic peptides for recognition, detection and live/dead differentiation of pathogenic bacteria.
X. Liu (2016)
10.1166/JBN.2014.1987
Applications of gold nanoparticles in optical biosensors.
L. Nie (2014)



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