Bifunctional Polyacrylamide Based Polymers For The Specific Binding Of Hexahistidine Tagged Proteins On Gold Surfaces.
L. Thompson, N. Mack, R. Nuzzo
Published 2010 · Medicine, Chemistry
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We describe a modified bifunctional analogue of polyacrylamide that spontaneously forms self-assembled polymeric thin films on Au surfaces. The film is engineered to specifically bind histidine tagged proteins (6His), while simultaneously remaining inherently resistant to the non-specific adsorption of proteins in solution. The backbone of a polyacrylamide-co-n-acryloxysuccinimide copolymer is functionalized via tandem active ester (NHS) couplings with 3-(methylthio)propylamine (MTP) and nitrilotriacetic acid (NTA). The resulting functionalized polymers form stable and exceptionally hydrophilic thin films that are approximately 2-5 nm thick, a mass coverage that varies with the MTP graft density. These films are characterized using a variety of techniques (X-ray photoelectron spectroscopy (XPS), reflection absorption infrared spectroscopy (RAIRS), ellipsometry, surface plasmon resonance (SPR), and matrix assisted laser desorption ionization (MALDI)) to establish their structure and function. The protein resistance of the films, as demonstrated by their exposure to solutions of bovine serum albumin (BSA), can be modulated by the amount of MTP grafted to the polymer, which in turn, affects their mass coverage. We show that it is possible to specifically capture hexahistidine tagged proteins with low incidences of nonspecific adsorption using these materials, a discrimination quantified using surface plasmon resonance (SPR) at concentrations down to approximately 20 nM. These polymers also bind strongly to the surfaces of Au nanoparticles, stabilizing them against aggregation, providing them with a similar capacity to selectively bind 6His tagged proteins that can then be speciated using MALDI.
This paper references
Surface-enhanced Raman scattering
K. Kneipp (2006)
Biomedical surface science: Foundations to frontiers
D. Castner (2002)
Polyethylene glycol-coated biocompatible surfaces.
N. Alcantar (2000)
A nanoscale optical biosensor: The long range distance dependence of the localized surface plasmon resonance of noble metal nanoparticles
A. J. Haes (2004)
The interaction of proteins with solid surfaces.
J. J. Gray (2004)
Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.
Farid Khan (2006)
Ni-nitrilotriacetic acid-modified quantum dots as a site-specific labeling agent of histidine-tagged proteins in live cells.
Junwon Kim (2008)
Preparation of Mixed Self-Assembled Monolayers (SAMs) That Resist Adsorption of Proteins Using the Reaction of Amines with a SAM That Presents Interchain Carboxylic Anhydride Groups
R. Chapman (2000)
Adsorption of Proteins to Hydrophobic Sites on Mixed Self-Assembled Monolayers†
E. Ostuni (2003)
Systems for the detection and analysis of protein–protein interactions
K. Kuroda (2006)
Formation and Patterning of Self-Assembled Monolayers Derived from Long-Chain Organosilicon Amphiphiles and Their Use as Templates in Materials Microfabrication
Krista R. Finnie (2000)
Quantitative imaging of protein adsorption on patterned organic thin-film arrays using secondary electron emission.
N. Mack (2006)
Molecular-Level Control over Surface Order in Self-Assembled Monolayer Films of Thiols on Gold
C. Bain (1988)
Polymeric Thin Films That Resist the Adsorption of Proteins and the Adhesion of Bacteria
R. Chapman (2001)
Nanoparticle-enhanced diffraction gratings for ultrasensitive surface plasmon biosensing.
A. Wark (2007)
MOLECULAR CONFORMATION AND SOLVATION OF OLIGO(ETHYLENE GLYCOL)-TERMINATED SELF-ASSEMBLED MONOLAYERS AND THEIR RESISTANCE TO PROTEIN ADSORPTION
R. Wang (1997)
Factors that determine the protein resistance of oligoether self-assembled monolayers --internal hydrophilicity, terminal hydrophilicity, and lateral packing density.
S. Herrwerth (2003)
Soft Lithographic Patterning of Hyaluronic Acid on Hydrophilic Substrates Using Molding and Printing
K. Suh (2004)
Self-Assembled Monolayers of Methyl 1-Thiahexa(ethylene oxide) for the Inhibition of Protein Adsorption
D. Vanderah (2002)
Optically tunable nanoparticle contrast agents for early cancer detection: model-based analysis of gold nanoshells.
A. W. H. Lin (2005)
A study of the nucleation and growth processes in the synthesis of colloidal gold
J. Turkevich (1951)
Synthesis of Nickel−Nitrilotriacetic Acid Coupled Single-Walled Carbon Nanotubes for Directed Self-Assembly with Polyhistidine-Tagged Proteins
R. A. Graff (2008)
Self-assembled monolayers containing terminal mono-, bis-, and tris-nitrilotriacetic acid groups: characterization and application.
R. Valiokas (2008)
Spontaneous Polymer Thin Film Assembly and Organization Using Mutually Immiscible Side Chains
F. Sun (1996)
Tailoring plasmonic substrates for surface enhanced spectroscopies.
Surbhi Lal (2008)
Notizen: Radiative Decay of Non Radiative Surface Plasmons Excited by Light
E. Kretschmann (1968)
SPR Studies of the Nonspecific Adsorption Kinetics of Human IgG and BSA on Gold Surfaces Modified by Self-Assembled Monolayers (SAMs)
Gold nanoparticles in biology: beyond toxicity to cellular imaging.
C. Murphy (2008)
Theoretical analysis of adsorption thermodynamics for hydrophobic peptide residues on SAM surfaces of varying functionality.
R. Latour (2002)
Size and temperature dependence of the plasmon absorption of colloidal gold nanoparticles
S. Link (1999)
High molecular weight hydrophilic functional copolymers by free‐radical copolymerization of acrylamide and of N‐acryloylmorpholine with N‐acryloxysuccinimide: Application to the synthesis of a graft copolymer
F. D'Agosto (2003)
XPS, TOF-SIMS, NEXAFS, and SPR characterization of nitrilotriacetic acid-terminated self-assembled monolayers for controllable immobilization of proteins.
F. Cheng (2008)
A highly stable nonbiofouling surface with well-packed grafted zwitterionic polysulfobetaine for plasma protein repulsion.
Yung Chang (2008)
Nanosensors based on responsive polymer brushes and gold nanoparticle enhanced transmission surface plasmon resonance spectroscopy.
I. Tokareva (2004)
Fabrication of histidine-tagged fusion protein arrays for surface plasmon resonance imaging studies of protein-protein and protein-DNA interactions.
G. Wegner (2003)
Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells.
M. Mrksich (1996)
Textural guidance cues for controlling process outgrowth of mammalian neurons.
J. N. Hanson (2009)
Magnetic nanoparticle design for medical diagnosis and therapy
S. Mornet (2004)
Resistance to nonspecific protein adsorption by poly(vinyl alcohol) thin films adsorbed to a poly(styrene) support matrix studied using surface plasmon resonance.
D. Barrett (2001)
Self-assembled monolayers of an oligo(ethylene oxide) disulfide and its corresponding thiol assembled from water: characterization and protein resistance.
D. Vanderah (2008)
Functionalizing Surfaces with Nickel Ions for the Grafting of Proteins
O. D. Roure (2003)
Assembly and Characterization of SAMs Formed by the Adsorption of Alkanethiols on Zinc Selenide Substrates
Alison R. Noble-Luginbuhl and (2001)
The interaction of proteins and cells with self-assembled monolayers of alkanethiolates on gold and silver
E. Ostuni (1999)
A self-assembled monolayer for the binding and study of histidine-tagged proteins by surface plasmon resonance.
G. Sigal (1996)
Kinase-catalyzed modification of gold nanoparticles: a new approach to colorimetric kinase activity screening.
Zhenxin Wang (2006)
Self-assembled monolayers and polymer brushes in biotechnology: current applications and future perspectives.
W. Senaratne (2005)
Dual-mode fluorophore-doped nickel nitrilotriacetic acid-modified silica nanoparticles combine histidine-tagged protein purification with site-specific fluorophore labeling.
S. Kim (2007)
A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein
E. Ostuni (2001)
Affinity purification of histidine-tagged proteins
J. Schmitt (2005)
Optical and surface‐analytical methods for the characterization of ultrathin organic films
C. Bubeck (1991)
Ni-NTA-gold clusters target His-tagged proteins.
J. Hainfeld (1999)
Nitrilotriacetic acid-coated magnetic nanoparticles as affinity probes for enrichment of histidine-tagged proteins and phosphorylated peptides.
Yi-cheng Li (2007)
Distance-dependent fluorescence quenching on gold nanoparticles ensheathed with layer-by-layer assembled polyelectrolytes.
G. Schneider (2006)
Surface adsorption and transfer of organomercaptans to colloidal gold and direct identification by matrix assisted laser desorption/ionization mass spectrometry.
J. S. Kirk (2004)
Quantitative interpretation of the response of surface plasmon resonance sensors to adsorbed films
Linda S. Jung (1998)
D. Davies (1990)
Enzyme immobilization by condensation copolymerization into crosslinked polyacrylamide gels
A. Pollak (1980)
Functionalized gold nanoparticles: synthesis, structure and colloid stability.
Jingfang Zhou (2009)
Self-assembled monolayers of thiolates on metals as a form of nanotechnology.
J. Love (2005)
Nitrilotriacetic Acid Functionalized Graft Copolymers: A Polymeric Interface for Selective and Reversible Binding of Histidine-Tagged Proteins†
Guoliang Zhen (2006)
Anisotropic metal nanoparticles: Synthesis, assembly, and optical applications.
C. Murphy (2005)
Multifunctional Monolayer Assemblies for Reversible Direct Fluorescence Transduction of Protein−Ligand Interactions at Surfaces
M. M. A. Sekar (1999)
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Alkylsulfanylphenyl derivatives of cytosine and 7-deazaadenine nucleosides, nucleotides and nucleoside triphosphates: synthesis, polymerase incorporation to DNA and electrochemical study.
Hana Macíčková-Cahová (2011)
Investigating organic multilayers by spectroscopic ellipsometry: specific and non-specific interactions of polyhistidine with NTA self-assembled monolayers
I. Solano (2016)
Freestanding Gold/Graphene-Oxide/Manganese Oxide Microsupercapacitor Displaying High Areal Energy Density.
Ahiud Morag (2017)
Protein coverage on polymer nanolayers leading to mesenchymal stem cell patterning.
Jungmok You (2011)
Chemical Strategies for the Synthesis of Protein–Polymer Conjugates
Björn Jung (2012)
Modern surface plasmon resonance for bioanalytics and biophysics.
M. Couture (2013)
Surface modification of luminescent lanthanide phosphate nanorods with cationic "Quat-primer" polymers.
Rajesh Komban (2011)
Transfer printing techniques for materials assembly and micro/nanodevice fabrication.
A. Carlson (2012)
Atomic Force Microscopy and Spectroscopic Ellipsometry combined analysis of Small Ubiquitin-like Modifier adsorption on functional monolayers
I. Solano (2017)
Activated Ester Containing Polymers: Opportunities and Challenges for the Design of Functional Macromolecules.
A. Das (2016)
Towards proteomics-on-chip: the role of the surface.
Malinda Salim (2011)
Building a sensitive immunosensing platform based on oriented immobilization of histidine-tagged antibody on NiO-decorated SWNTs
Hongxia Chen (2013)
Immobilization of bio-macromolecules on self-assembled monolayers: methods and sensor applications.
Debasis Samanta (2011)