← Back to Search
Strategies For The Intracellular Delivery Of Nanoparticles.
L. Chou, Kevin Ming, W. Chan
Published 2011 · Chemistry, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
The ability to target contrast agents and therapeutics inside cells is becoming important as we strive to decipher the complex network of events that occur within living cells and design therapies that can modulate these processes. Nanotechnology researchers have generated a growing list of nanoparticles designed for such applications. These particles can be assembled from a variety of materials into desirable geometries and configurations and possess useful properties and functionalities. Undoubtedly, the effective delivery of these nanomaterials into cells will be critical to their applications. In this tutorial review, we discuss the fundamental challenges of delivering nanoparticles into cells and to the targeted organelles, and summarize strategies that have been developed to-date.
This paper references
High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling.
S. Soenen (2010)
Poly(L-lysine)-modified iron oxide nanoparticles for stem cell labeling.
M. Babič (2008)
Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells.
B. Chithrani (2006)
Cellular trajectories of peptide-modified gold particle complexes: comparison of nuclear localization signals and peptide transduction domains.
A. Tkachenko (2004)
Efficient gene delivery targeted to the brain using a transferrin‐conjugated polyethyleneglycol‐modified polyamidoamine dendrimer
Rong-qin Huang (2007)
Cell-penetrating peptides as delivery vehicles for biology and medicine.
Kelly M. Stewart (2008)
Aptamer-conjugated nanoparticles for the collection and detection of multiple cancer cells.
J. Smith (2007)
Lipid-gold-nanoparticle hybrid-based gene delivery.
W. Rhim (2008)
Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery.
M. A. Herrero (2009)
Single chain epidermal growth factor receptor antibody conjugated nanoparticles for in vivo tumor targeting and imaging.
Lily Yang (2009)
Intracellular delivery of quantum dot-protein cargos mediated by cell penetrating peptides.
Igor L. Medintz (2008)
Imaging viral behavior in Mammalian cells with self-assembled capsid-quantum-dot hybrid particles.
F. Li (2009)
Twenty years of cell-penetrating peptides: from molecular mechanisms to therapeutics
Frédéric Heitz (2009)
Understanding biophysicochemical interactions at the nano-bio interface.
A. Nel (2009)
Monoclonal antibody therapy of cancer
G. Adams (2005)
Cellular trafficking of quantum dot-ligand bioconjugates and their induction of changes in normal routing of unconjugated ligands.
C. Tekle (2008)
Proton-sponge coated quantum dots for siRNA delivery and intracellular imaging.
M. Yezhelyev (2008)
Dendrimer-entrapped gold nanoparticles as a platform for cancer-cell targeting and imaging.
X. Shi (2007)
Delivering quantum dots into cells: strategies, progress and remaining issues
J. Delehanty (2009)
Multifunctional magneto-polymeric nanohybrids for targeted detection and synergistic therapeutic effects on breast cancer.
Jaemoon Yang (2007)
F. Laforge (2007)
Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals.
D. Chatterjee (2008)
DNA aptamer–micelle as an efficient detection/delivery vehicle toward cancer cells
Y. Wu (2009)
Endocytic mechanisms for targeted drug delivery.
Lisa Bareford (2007)
Target-specific cellular uptake of PLGA nanoparticles coated with poly(L-lysine)-poly(ethylene glycol)-folate conjugate.
S. H. Kim (2005)
PLGA nanoparticle--peptide conjugate effectively targets intercellular cell-adhesion molecule-1.
N. Zhang (2008)
Gold hybrid nanoparticles for targeted phototherapy and cancer imaging.
D. Kirui (2010)
Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and
F. Chen (2004)
Cytosolic targeting of macromolecules using a pH-dependent fusogenic peptide in combination with cationic liposomes.
S. Kobayashi (2009)
Targeted delivery of anti-CD19 liposomal doxorubicin in B-cell lymphoma: a comparison of whole monoclonal antibody, Fab' fragments and single chain Fv.
W. Cheng (2008)
Biodegradable quantum dot nanocomposites enable live cell labeling and imaging of cytoplasmic targets.
Betty Y. S. Kim (2008)
Synthesis and grafting of thioctic acid-PEG-folate conjugates onto Au nanoparticles for selective targeting of folate receptor-positive tumor cells.
Vivechana Dixit (2006)
Nanoparticles functionalized with recombinant single chain Fv antibody fragments (scFv) for the magnetic resonance imaging of cancer cells.
K. L. Vigor (2010)
Dendrimer-epidermal growth factor conjugate displays superagonist activity.
T. Thomas (2008)
Knocking down barriers: advances in siRNA delivery
K. Whitehead (2009)
Targeted quantum dot conjugates for siRNA delivery.
Austin M. Derfus (2007)
Transferrin-modified liposomes equipped with a pH-sensitive fusogenic peptide: an artificial viral-like delivery system.
Tomoyuki Kakudo (2004)
Uptake and intracellular fate of surface-modified gold nanoparticles.
P. Nativo (2008)
Folate-receptor-mediated delivery of InP quantum dots for bioimaging using confocal and two-photon microscopy.
D. Bharali (2005)
Regulated portals of entry into the cell
S. Conner (2003)
A nonviral transfection approach in vitro: the design of a gold nanoparticle vector joint with microelectromechanical systems.
C. Jen (2004)
Polyplex micelles with cyclic RGD peptide ligands and disulfide cross-links directing to the enhanced transfection via controlled intracellular trafficking.
M. Oba (2008)
Why gold nanoparticles are more precious than pretty gold: noble metal surface plasmon resonance and its enhancement of the radiative and nonradiative properties of nanocrystals of different shapes.
S. Eustis (2006)
Covalently dye-linked, surface-controlled, and bioconjugated organically modified silica nanoparticles as targeted probes for optical imaging.
R. Kumar (2008)
Multifunctional polymeric micelles as cancer-targeted, MRI-ultrasensitive drug delivery systems.
N. Nasongkla (2006)
Targeted delivery of cisplatin to prostate cancer cells by aptamer functionalized Pt(IV) prodrug-PLGA–PEG nanoparticles
Shanta Dhar (2008)
Preparation and characterization of PE38KDEL-loaded anti-HER2 nanoparticles for targeted cancer therapy.
H. Chen (2008)
Nuclear targeting of gold nanoparticles in cancer cells induces DNA damage, causing cytokinesis arrest and apoptosis.
B. Kang (2010)
Nanoparticle interaction with biological membranes: does nanotechnology present a Janus face?
Pascale R. Leroueil (2007)
Cell-penetrating quantum dots based on multivalent and endosome-disrupting surface coatings.
H. Duan (2007)
Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes.
B. Chithrani (2007)
Aptamer-based silver nanoparticles used for intracellular protein imaging and single nanoparticle spectral analysis.
L. Chen (2010)
Intracellular delivery of quantum dots tagged antisense oligodeoxynucleotides by functionalized multiwalled carbon nanotubes.
Nengqin Jia (2007)
Semiconductor nanocrystals: structure, properties, and band gap engineering.
A. M. Smith (2010)
Probing cellular events, one quantum dot at a time
F. Pinaud (2010)
Arming antibodies: prospects and challenges for immunoconjugates
A. Wu (2005)
Fluorescence-modified superparamagnetic nanoparticles: intracellular uptake and use in cellular imaging.
F. Bertorelle (2006)
Conjugation to gold nanoparticles enhances polyethylenimine's transfer of plasmid DNA into mammalian cells
Mini P. Thomas (2003)
LHRH-conjugated Magnetic Iron Oxide Nanoparticles for Detection of Breast Cancer Metastases
C. Leuschner (2006)
Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties
S. Kumar (2008)
Phagocytosis: elegant complexity.
L. Stuart (2005)
Intracellular bioconjugation of targeted proteins with semiconductor quantum dots.
K. Boeneman (2010)
Microinjection as a tool of mechanical delivery.
Y. Zhang (2008)
Carbon nanosyringe array as a platform for intracellular delivery.
Sangjin Park (2009)
Folate receptor-mediated drug targeting: from therapeutics to diagnostics.
A. Hilgenbrink (2005)
Nanoparticle-mediated cellular response is size-dependent.
W. Jiang (2008)
Imaging and tracking of tat peptide-conjugated quantum dots in living cells: new insights into nanoparticle uptake, intracellular transport, and vesicle shedding.
G. Ruan (2007)
Engineered antibody fragments and the rise of single domains
P. Holliger (2005)
Pathways of clathrin-independent endocytosis
S. Mayor (2007)
Multifunctional lipid/quantum dot hybrid nanocontainers for controlled targeting of live cells.
Gopakumar Gopalakrishnan (2006)
Mechanisms of cell death in oxidative stress.
S. Ryter (2007)
Inhibition of tumor-cell invasion with chlorotoxin-bound superparamagnetic nanoparticles.
O. Veiseh (2009)
Magnetic nanoparticles: synthesis, protection, functionalization, and application.
A. Lu (2007)
Solute and macromolecule diffusion in cellular aqueous compartments.
A. Verkman (2002)
Distinct endocytic pathways regulate TGF-β receptor signalling and turnover
G. D. Guglielmo (2003)
FluidFM: combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond.
A. Meister (2009)
Functionalized-quantum-dot-liposome hybrids as multimodal nanoparticles for cancer.
W. T. Al-Jamal (2008)
Synthesis of cetuximab-immunoliposomes via a cholesterol-based membrane anchor for targeting of EGFR.
Xiaogang Pan (2007)
Assessing the effect of surface chemistry on gold nanorod uptake, toxicity, and gene expression in mammalian cells.
Tanya S. Hauck (2008)
Cys-diabody quantum dot conjugates (immunoQdots) for cancer marker detection.
B. Barat (2009)
Mechanism of active targeting in solid tumors with transferrin-containing gold nanoparticles
C. Choi (2009)
Delivery of macromolecules into living cells: a method that exploits folate receptor endocytosis.
C. P. Leamon (1991)
Intracellular delivery of core-shell fluorescent silica nanoparticles.
Jason E. Fuller (2008)
Lipid Rafts and Caveolae as Portals for Endocytosis: New Insights and Common Mechanisms
R. Parton (2003)
Plasmonic nanosensors for imaging intracellular biomarkers in live cells.
S. Kumar (2007)
Attaching folic acid on gold nanoparticles using noncovalent interaction via different polyethylene glycol backbones and targeting of cancer cells
Resham Bhattacharya (2007)
Tat peptide as an efficient molecule to translocate gold nanoparticles into the cell nucleus.
J. M. de la Fuente (2005)
Intracellular Delivery of Quantum Dots for Live Cell Labeling and Organelle Tracking
Austin M. Derfus (2004)
The endocytic matrix
G. Scita (2010)
Folic acid-PEG conjugated superparamagnetic nanoparticles for targeted cellular uptake and detection by MRI.
C. Sun (2006)
Multifunctional polymeric micelles with folate-mediated cancer cell targeting and pH-triggered drug releasing properties for active intracellular drug delivery.
Y. Bae (2005)
Macrophage receptors and immune recognition.
P. Taylor (2005)
Tat peptide-mediated intracellular delivery of pharmaceutical nanocarriers.
V. Torchilin (2008)
A Nucleolin-Targeted Multimodal Nanoparticle Imaging Probe for Tracking Cancer Cells Using an Aptamer
D. Hwang (2010)
Magnetic nanoparticle-peptide conjugates for in vitro and in vivo targeting and extraction of cancer cells.
K. E. Scarberry (2008)
Nanoparticle-mediated IgE-receptor aggregation and signaling in RBL mast cells.
Y. Huang (2009)
Endocytosis unplugged: multiple ways to enter the cell
Sudha Kumari (2010)
Nanoplatforms for targeted molecular imaging in living subjects.
W. Cai (2007)
One at a time, live tracking of NGF axonal transport using quantum dots
B. Cui (2007)
The many ways to cross the plasma membrane
D. Stephens (2001)
Nanoneedle: a multifunctional tool for biological studies in living cells.
Kyungsuk Yum (2010)
Conscripts of the infinite armada: systemic cancer therapy using nanomaterials
D. Scheinberg (2010)
Cell-specific targeting of nanoparticles by multivalent attachment of small molecules
R. Weissleder (2005)
S. A. Mousavi (2004)
Lipid-quantum dot bilayer vesicles enhance tumor cell uptake and retention in vitro and in vivo.
W. T. Al-Jamal (2008)
Correlative microscopy: bridging the gap between fluorescence light microscopy and cryo-electron tomography.
Anna Sartori (2007)
Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery
I. Khalil (2006)
Mechanisms of pathogen entry through the endosomal compartments
J. Gruenberg (2006)
PEI-PEG-Chitosan Copolymer Coated Iron Oxide Nanoparticles for Safe Gene Delivery: synthesis, complexation, and transfection.
Forrest M Kievit (2009)
Learning from the Viral Journey: How to Enter Cells and How to Overcome Intracellular Barriers to Reach the Nucleus
Diky Mudhakir (2009)
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
B. Dubertret (2002)
Polymers for gene delivery across length scales
D. Putnam (2006)
Clathrin-coated vesicle formation and protein sorting: an integrated process.
S. Schmid (1997)
In vivo quantum dot labeling of mammalian stem and progenitor cells
Jonathan R. Slotkin (2007)
In vitro selection of RNA molecules that bind specific ligands
A. Ellington (1990)
Cholera toxin B conjugated quantum dots for live cell labeling.
S. Chakraborty (2007)
This paper is referenced by
A Systems Toxicology Approach to Investigating the Cardiovascular Effects of Cigarette Smoke and Environmental Pollutants in ApoE -Deficient Mice
Hector Ramirez de Leon (2015)
Human and eco-toxicological impacts of organometallic halide perovskites
Ryma Iness Benmessaoud (2017)
Design of an acid labile traceless-cleavable click linker for use in a novel protein transduction shuttle
Kevin Maier (2012)
Intracellular delivery of nanocarriers and targeting to subcellular organelles
Aditi M. Jhaveri (2016)
Ternary hybrid nanocomposites for gene delivery and magnetic resonance imaging of hepatocellular carcinoma cells.
K. Leung (2013)
Strategies for the Intracellular Delivery of Nanoparticles
L. Chou (2011)
Quantitative measurement of quantum dot uptake at the cell population level using microfluidic evanescent-wave-based flow cytometry.
J. Wang (2012)
Primary ammonium monocarboxylate synthon in designing supramolecular gels: a new series of chiral low-molecular-weight gelators derived from simple organic salts that are capable of generating and stabilizing gold nanoparticles.
U. Das (2013)
pH‐responsive near‐infrared emitting conjugated polymer nanoparticles for cellular imaging and controlled‐drug delivery
Jousheed Pennakalathil (2015)
Nanostructured porous Si-based nanoparticles for targeted drug delivery
M. Shahbazi (2012)
Applications of Nanomaterials for Immunosensing
Sandra Lara (2018)
Megakaryocyte-membrane wrapped nanoparticles for targeted delivery to hematopoietic stem cells
E. Winter (2018)
Bone Regeneration from PLGA Micro-Nanoparticles
I. Ortega-Oller (2015)
Efficient Condensation of DNA into Environmentally Responsive Polyplexes Produced from Block Catiomers Carrying Amine or Diamine Groups.
Lindomar J C Albuquerque (2016)
Toxicological profile of lipid-based nanostructures: are they considered as completely safe nanocarriers?
Asaad Azarnezhad (2020)
The Role of the Shape in the Design of New Nanoparticles
G. M. Estrada-Villegas (2014)
In Vivo Behavior of Nanoparticles : Impact of Surface Modifications on the Spatiotemporal Microdistribution of Quantum Dots
U. Pohl (2017)
Uptake of engineered gold nanoparticles into mammalian cells.
L. Dykman (2014)
Synthesis of 68Ga-Labeled Biopolymer-based Nanoparticle Imaging Agents for Positron-emission Tomography
Zoltán Körhegyi (2019)
Uptake of poly(2-hydroxypropylmethacrylamide)-coated gold nanoparticles in microvascular endothelial cells and transport across the blood-brain barrier.
C. Freese (2013)
Single cell optical imaging and spectroscopy.
Anthony S Stender (2013)
Inorganic Nanoparticle-Based Smart Drug Delivery Systems
Jutaek Nam (2016)
Detection of DNA Damage Induced by Cerium Dioxide Nanoparticles: From Models to Molecular Mechanism Activated.
T. A. J. de Souza (2018)
Pharmacological Behavior of Systemically Administered Nanoparticles of Defined Properties: Mechanistic Investigations at the Organ, Tissue, and Cellular Levels
C. Choi (2011)
Smart Self-Assembled Nanosystem Based on Water-Soluble Pillararene and Rare-Earth-Doped Upconversion Nanoparticles for pH-Responsive Drug Delivery.
H. Li (2018)
Temperature-sensitive polypeptide nanogels for intracellular delivery of a biomacromolecular drug.
Du Young Ko (2015)
Molecular analysis of interactions between dendrimers and asymmetric membranes at different transport stages.
X. He (2014)
Development of analytical methods for functional analysis of intracellular protein using signal-responsive silica or organic nanoparticles.
Masaru Kato (2016)
Present and Future of Surface Enhanced Raman Scattering.
J. Langer (2019)
Intracellular cargo delivery by virus capsid protein-based vehicles: From nano to micro.
D. Gao (2016)
Nanoparticle opsonization: forces involved and protection by long chain polymers
T. U. Wani (2019)
N-acetyl-D-glucosamine-conjugated PAMAM Dendrimers as Dual Receptor-Targeting Nanocarriers for Anticancer Drug Delivery.
D. Pooja (2020)See more