Mechanistic Studies Of The Reactions Of The Reduced Vitamin B12 Derivatives With The HNO Donor Piloty's Acid: Further Evidence For Oxidation Of Cob(i)alamin By (H)NO

Kinetic data and product analyses support a mechanism in which cob(II)alamin (Cbl(ii)˙) is reduced by (H)NO to give cob(i)alamin (Cbl(i)⁻) and ˙NO. Cbl(i)⁻ is subsequently oxidized back to Cbl(ii)˙ by a second (H)NO molecule, and Cbl(ii)˙ reacts rapidly with ˙NO to form nitroxylcobalamin, NOCbl.